RchiOBHm_Chr1g0331311

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0020436
Physical Location & Seq
Forward (+)
21145183 .. 21148437
3255 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
ATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTATTCGAGCCCTAGGCAGGAACACTAGGGTTTCTATAGCCATGCGACTTAAAGAAGGCGATAAGATGGCAAGCGTAGAGATTATACCAGCAGCTATGCGGAAAGACTTGGAGAGGGTGTCAGAAGCACCAGAAAGCACGGCTAGAAGTCTGAAAGGCCCATGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCATCTGAACAGGGTGGGTCTGATAGGTTACAAGTTTTCCGCGGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGGTGGTGAAAGTGATGAACAAGTGGTCCTAGTAAGCCAAAGTGGTACTGTGAATAGAATTAAGGTGCGGGATATCTCAATACAGTCTCGCTATGCAAGCACATTTTCCAGGGGTATTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCCGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAATCTGCAATTCATGGTTAA

Protein Analysis

195

Amino Acids

21.16

Weight (kDa)

6.85

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_gyraseA_C PF03989 69 - 114 1.4e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020436)

Species Orthologous Gene IDs
pyrus_communis pycom07g04210
rosa_chinensis RchiOBHm_Chr1g0331311
rosa_multiflora Rmu_sc0005956.1_g000014 Rmu_sc0005956.1_g000017 Rmu_sc0011261.1_g000004
rosa_samantha Rh1AG117400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AccII CGCG 1 cut(s) 311
AciI CCGC 4 cut(s) 142, 309, 311, 427
AfaI GTAC 1 cut(s) 406
AfiI CCNNNNNNNGG 1 cut(s) 60
AgsI TTSAA 1 cut(s) 557
AjnI CCWGG 1 cut(s) 467
AloI GAACNNNNNNTCC 4 cut(s) 228, 260, 369, 401
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 450
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 2 cut(s) 134, 326
AseI ATTAAT 1 cut(s) 524
AspA2I CCTAGG 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 200, 316, 385
AsuHPI GGTGA 1 cut(s) 377
AvaII GGWCC 2 cut(s) 316, 385
AvrII CCTAGG 1 cut(s) 55
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 55
BbvI GCAGC 2 cut(s) 146, 338
BccI CCATC 2 cut(s) 103, 279
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 4 cut(s) 56, 69, 186, 389
BfmI CTRYAG 1 cut(s) 78
BisI GCNGC 2 cut(s) 135, 327
BlnI CCTAGG 1 cut(s) 55
BlsI GCNGC 2 cut(s) 136, 328
Bme1390I CCNGG 1 cut(s) 469
Bme18I GGWCC 2 cut(s) 316, 385
BmgT120I GGNCC 3 cut(s) 200, 316, 385
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 1 cut(s) 469
BmsI GCATC 2 cut(s) 14, 474
BpuEI CTTGAG 1 cut(s) 271
BsaJI CCNNGG 4 cut(s) 55, 203, 309, 468
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 4 cut(s) 55, 203, 309, 468
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 213
BseXI GCAGC 2 cut(s) 146, 338
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 544
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 450
BsnI GGCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 1 cut(s) 493
Bsp19I CCATGG 1 cut(s) 203
BspACI CCGC 4 cut(s) 142, 309, 311, 427
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 1 cut(s) 546
BspT107I GGYRCC 1 cut(s) 544
BssECI CCNNGG 4 cut(s) 55, 203, 309, 468
BssMI GATC 1 cut(s) 493
BssT1I CCWWGG 2 cut(s) 55, 203
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 2 cut(s) 409, 444
Bst6I CTCTTC 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 115, 457
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 2 cut(s) 203, 309
BstF5I GGATG 1 cut(s) 5
BstFNI CGCG 1 cut(s) 311
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 1 cut(s) 493
BstMWI GCNNNNNNNGC 1 cut(s) 456
BstNI CCWGG 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 467
BstSFI CTRYAG 1 cut(s) 78
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 2 cut(s) 146, 338
BsuRI GGCC 1 cut(s) 201
BtgI CCRYGG 2 cut(s) 203, 309
BtsCI GGATG 1 cut(s) 5
Cac8I GCNNGC 2 cut(s) 115, 457
Cfr13I GGNCC 3 cut(s) 200, 316, 385
Cfr42I CCGCGG 1 cut(s) 312
Csp6I GTAC 1 cut(s) 405
CviAII CATG 3 cut(s) 85, 204, 581
CviJI RGCY 6 cut(s) 53, 83, 137, 185, 201, 396
CviKI_1 RGCY 6 cut(s) 53, 83, 137, 185, 201, 396
CviQI GTAC 1 cut(s) 405
DdeI CTNAG 1 cut(s) 222
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 55, 203
Eco24I GRGCYC 1 cut(s) 55
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 2 cut(s) 316, 385
EcoRII CCWGG 1 cut(s) 467
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 55, 203
EcoT38I GRGCYC 1 cut(s) 55
ErhI CCWWGG 2 cut(s) 55, 203
FaeI CATG 3 cut(s) 88, 207, 584
FaiI YATR 9 cut(s) 31, 80, 86, 128, 140, 205, 234, 453, 582
FatI CATG 3 cut(s) 84, 203, 580
FauI CCCGC 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 135, 327
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 2 cut(s) 135, 327
FspBI CTAG 4 cut(s) 56, 69, 186, 389
GluI GCNGC 2 cut(s) 135, 327
HaeIII GGCC 1 cut(s) 201
Hin1II CATG 3 cut(s) 88, 207, 584
HinfI GANTC 4 cut(s) 345, 489, 508, 569
HphI GGTGA 1 cut(s) 377
Hpy188I TCNGA 6 cut(s) 40, 166, 195, 223, 276, 291
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 2 cut(s) 409, 444
HpyCH4V TGCA 2 cut(s) 455, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 456
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 3 cut(s) 88, 207, 584
KspI CCGCGG 1 cut(s) 312
Kzo9I GATC 1 cut(s) 493
LpnPI CCDG 8 cut(s) 46, 144, 186, 265, 454, 481, 486, 561
Lsp1109I GCAGC 2 cut(s) 146, 338
LweI GCATC 2 cut(s) 14, 474
MaeI CTAG 4 cut(s) 56, 69, 186, 389
MaeIII GTNAC 1 cut(s) 296
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 1 cut(s) 258
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 2 cut(s) 417, 576
MlyI GAGTC 1 cut(s) 483
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 4 cut(s) 150, 307, 352, 535
MseI TTAA 4 cut(s) 93, 420, 524, 586
MspA1I CMGCKG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 469
MvaI CCWGG 1 cut(s) 469
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 456
NcoI CCATGG 1 cut(s) 203
NdeII GATC 1 cut(s) 493
NlaIII CATG 3 cut(s) 88, 207, 584
NlaIV GGNNCC 1 cut(s) 546
PfeI GAWTC 3 cut(s) 345, 508, 569
PkrI GCNGC 2 cut(s) 136, 328
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
PshBI ATTAAT 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 546
PspPI GGNCC 3 cut(s) 200, 316, 385
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
SacII CCGCGG 1 cut(s) 312
SaqAI TTAA 4 cut(s) 93, 420, 524, 586
SatI GCNGC 2 cut(s) 135, 327
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 3 cut(s) 200, 316, 385
SchI GAGTC 1 cut(s) 483
ScrFI CCNGG 1 cut(s) 469
SduI GDGCHC 1 cut(s) 55
SetI ASST 4 cut(s) 139, 298, 426, 546
SfaNI GCATC 2 cut(s) 14, 474
SfcI CTRYAG 1 cut(s) 78
Sfr303I CCGCGG 1 cut(s) 312
SgrBI CCGCGG 1 cut(s) 312
SinI GGWCC 2 cut(s) 316, 385
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 2 cut(s) 417, 576
SsiI CCGC 4 cut(s) 142, 309, 311, 427
SspMI CTAG 4 cut(s) 56, 69, 186, 389
StyD4I CCNGG 1 cut(s) 467
StyI CCWWGG 2 cut(s) 55, 203
TaaI ACNGT 2 cut(s) 409, 444
TaqI TCGA 2 cut(s) 49, 492
TaqII GACCGA 1 cut(s) 333
TasI AATT 2 cut(s) 417, 576
TfiI GAWTC 3 cut(s) 345, 508, 569
Tru1I TTAA 4 cut(s) 93, 420, 524, 586
Tru9I TTAA 4 cut(s) 93, 420, 524, 586
TseI GCWGC 2 cut(s) 134, 326
TspDTI ATGAA 4 cut(s) 390, 510, 569, 582
TspGWI ACGGA 2 cut(s) 30, 501
VpaK11BI GGWCC 2 cut(s) 316, 385
VspI ATTAAT 1 cut(s) 524
XmaJI CCTAGG 1 cut(s) 55
XspI CTAG 4 cut(s) 56, 69, 186, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.