RchiOBHm_Chr1g0350871

Fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014373
Physical Location & Seq
Reverse (-)
44027740 .. 44030096
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1275 bp
ATGGCCTCCTCCCGCTGCCACCTCCTCCTCCTCACTCTCCTCTCCCTCGCCGCCGCCATCTCCGCCCACAACATCACCGCCATTCTCGAAGCCAACCCCGAGTACAGCCAGTACAACTCCTTCTTAACCCAAACAAGACTAGCCGACGAGATCAACACCCGCACCACCGTCACCGTCCTCGTCCTCACCAATGGCGTCATGTCATCCCTCGCCGCCAAGCACCCCCTCTCCGTCATCAAGAACGCTCTCAGCCTCCACATCCTTCTCGACTACTACGACCCCGCCAAGCTCCACAAAATCTCCGACGGCTCCGTCCTCACCACCACCCTCTACCAGACCACCGGCCACGCCCCCGGCAACCTCGGCTTCGTCAACATCACCGATCTCTCCGGCGGCAAAGTCGGCTTCGGCTCCGCCGCCCCCGGATCCAAGCTCGACTCCAGCTACACTAAATCCGTCAAGCAGATTCCCTACAACATCTCCGTCCTCGAGATCTCCGCCCCGGTCATCGCCCCCGGCATCTTGACCGCTCCGGCCCCGTCGGCCTCGGATTTGAACATCACCGCGCTTCTAGAAAAAGCCGGGTGCAAAACCTTCGCTTCTTTAATCGTCTCCAGCGGGGTGCTCAAGACTTTCCAGACCACCGCCGATAAGGGATTGACTCTGTTTGTGCCGAACGACGAGGCGTTTAAGGCCAAGGGCGTGCCGGATCTGACCAAGCTCACCAATGCCGAGGTCGTTTCGCTCTTGCAGTACCACGCCGTCCCTAAATACACTCCCAAAGGTGCCCTCAAGACCACCAAGGATCCAATCAGCACTCTCGCCACCAATGGCGCCGGAAAGTACGACCTGACCGTCACCACCTCCGGTGACCAGGTCACACTCCACACCGGAGTCGTCTCCTCCAGAGTCGCCGACACTGTCCTCGACTCTACTCCGTTTTCAGTCTTCACTGTCGACAGTGTTCTTCTCCCAACTGAGCTCTTCGGCAAATCTCCATCGCCGGCGCCGTCGATGGAGCCGGTGGCTGCTCCTTCACCGACACCAGTCGTTGCTCCCAGCCCAGCTTCCGTAGAAGCGCCGTCGCCGTCGGCCGCTTCACCGCCGGCTCCTCCTTCCGAGACACCGGAGGGAGCTCCGTCGGATGCACCGTCGGCAGCGGCGGCGGACAGTACCTCGGCGAACGGAGCTGGTCACGTGACTTCACCTGCATTGTTGATGACAGTGCTCACCGTCGCCTCTGTGACTGTCATTTCCTTCGTTTTCTTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0005886 GO:0009505 GO:0016020 GO:0030312 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fasciclin

Protein Analysis

424

Amino Acids

43.53

Weight (kDa)

5.97

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 195 - 325 1.8e-19 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014373)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45470 AT3G60900
fragaria_vesca FvH4_7g12120
malus_domestica MD02G1201100.v1.1
prunus_persica Prupe.2G147700_v2.0.a1
pyrus_communis pycom02g16450
rosa_chinensis RchiOBHm_Chr1g0350871
rosa_laevigata RLG00000028447
rosa_multiflora Rmu_co8189458.1_g000001 Rmu_co8311065.1_g000001
rosa_roxburghii Rroxscaffold_4G00304450
rosa_rugosa Rorug01G0214800
rosa_samantha Rh1AG229200 Rh1BG199200 Rh1CG214000 Rh1DG227100
rosa_wichuraiana Rw1G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1216
AasI GACNNNNNNGTC 2 cut(s) 311, 854
Acc36I ACCTGC 1 cut(s) 1216
AccB1I GGYRCC 3 cut(s) 785, 833, 1006
AccBSI CCGCTC 1 cut(s) 530
AccI GTMKAC 1 cut(s) 957
AccII CGCG 1 cut(s) 566
AclWI GGATC 5 cut(s) 420, 433, 717, 800, 813
AcoI YGGCCR 2 cut(s) 343, 1092
AcvI CACGTG 1 cut(s) 1198
AcyI GRCGYC 3 cut(s) 195, 834, 1007
AfaI GTAC 5 cut(s) 104, 113, 755, 845, 1174
AgsI TTSAA 1 cut(s) 556
AjnI CCWGG 1 cut(s) 873
AluBI AGCT 8 cut(s) 289, 433, 444, 721, 982, 1067, 1136, 1190
AluI AGCT 8 cut(s) 289, 433, 444, 721, 982, 1067, 1136, 1190
Alw21I GWGCWC 4 cut(s) 627, 984, 1138, 1230
Alw26I GTCTC 3 cut(s) 616, 904, 1115
AlwI GGATC 5 cut(s) 420, 433, 717, 800, 813
Ama87I CYCGRG 2 cut(s) 98, 488
AoxI GGCC 6 cut(s) 3, 343, 534, 543, 693, 1092
ApeKI GCWGC 3 cut(s) 15, 1028, 1157
AspLEI GCGC 4 cut(s) 568, 836, 1009, 1081
AspS9I GGNCC 1 cut(s) 535
AsuC2I CCSGG 5 cut(s) 354, 423, 503, 516, 583
AvaI CYCGRG 2 cut(s) 98, 488
BaeGI GKGCMC 1 cut(s) 790
BamHI GGATCC 2 cut(s) 425, 805
BanI GGYRCC 3 cut(s) 785, 833, 1006
BanII GRGCYC 2 cut(s) 984, 1138
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BbrPI CACGTG 1 cut(s) 1198
BbsI GAAGAC 1 cut(s) 940
Bbv12I GWGCWC 4 cut(s) 627, 984, 1138, 1230
BbvI GCAGC 3 cut(s) 2, 1015, 1169
BccI CCATC 3 cut(s) 65, 1006, 1009
BceAI ACGGC 5 cut(s) 322, 746, 994, 1066, 1072
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 1 cut(s) 875
BcnI CCSGG 5 cut(s) 354, 423, 503, 516, 583
BcoDI GTCTC 3 cut(s) 616, 904, 1115
BfaI CTAG 2 cut(s) 140, 572
BfoI RGCGCY 3 cut(s) 837, 1010, 1082
BfuAI ACCTGC 1 cut(s) 1216
BglI GCCNNNNNGGC 1 cut(s) 542
BglII AGATCT 1 cut(s) 492
Bme1390I CCNGG 6 cut(s) 354, 423, 503, 516, 583, 875
BmeT110I CYCGRG 2 cut(s) 98, 488
BmgT120I GGNCC 1 cut(s) 535
BmrFI CCNGG 6 cut(s) 354, 423, 503, 516, 583, 875
BmsI GCATC 2 cut(s) 528, 1135
BoxI GACNNNNGTC 1 cut(s) 1046
BpiI GAAGAC 1 cut(s) 940
BpmI CTGGAG 3 cut(s) 424, 598, 889
BpuEI CTTGAG 2 cut(s) 611, 776
BpuMI CCSGG 5 cut(s) 354, 423, 503, 516, 583
BsaAI YACGTR 1 cut(s) 1198
BsaHI GRCGYC 3 cut(s) 195, 834, 1007
BsaWI WCCGGW 3 cut(s) 866, 890, 1126
BsaXI ACNNNNNCTCC 6 cut(s) 212, 242, 284, 314, 464, 494
Bse118I RCCGGY 4 cut(s) 341, 1003, 1021, 1105
Bse1I ACTGG 2 cut(s) 109, 1046
BseBI CCWGG 1 cut(s) 875
BseGI GGATG 3 cut(s) 203, 258, 1150
BseMII CTCAG 2 cut(s) 262, 969
BseNI ACTGG 2 cut(s) 109, 1046
BseRI GAGGAG 6 cut(s) 14, 17, 20, 29, 892, 1101
BseSI GKGCMC 1 cut(s) 790
BseX3I CGGCCG 1 cut(s) 1092
BseXI GCAGC 3 cut(s) 2, 1015, 1169
BseYI CCCAGC 2 cut(s) 1058, 1063
Bsh1236I CGCG 1 cut(s) 566
Bsh1285I CGRYCG 1 cut(s) 1095
BshFI GGCC 6 cut(s) 5, 345, 536, 545, 695, 1094
BshNI GGYRCC 3 cut(s) 785, 833, 1006
BsiEI CGRYCG 1 cut(s) 1095
BsiHKAI GWGCWC 4 cut(s) 627, 984, 1138, 1230
BsiHKCI CYCGRG 2 cut(s) 98, 488
BslFI GGGAC 1 cut(s) 749
BsmAI GTCTC 3 cut(s) 616, 904, 1115
BsmBI CGTCTC 2 cut(s) 616, 904
BsmFI GGGAC 1 cut(s) 749
BsnI GGCC 6 cut(s) 5, 345, 536, 545, 695, 1094
BsoBI CYCGRG 2 cut(s) 98, 488
Bsp1286I GDGCHC 5 cut(s) 627, 790, 984, 1138, 1230
Bsp143I GATC 6 cut(s) 150, 382, 425, 492, 709, 805
BspANI GGCC 6 cut(s) 5, 345, 536, 545, 695, 1094
BspCNI CTCAG 2 cut(s) 261, 970
BspFNI CGCG 1 cut(s) 566
BspMI ACCTGC 1 cut(s) 1216
BspPI GGATC 5 cut(s) 420, 433, 717, 800, 813
BspQI GCTCTTC 1 cut(s) 989
BspT107I GGYRCC 3 cut(s) 785, 833, 1006
BsrBI CCGCTC 1 cut(s) 530
BsrFI RCCGGY 4 cut(s) 341, 1003, 1021, 1105
BsrI ACTGG 2 cut(s) 109, 1046
BssAI RCCGGY 4 cut(s) 341, 1003, 1021, 1105
BssMI GATC 6 cut(s) 150, 382, 425, 492, 709, 805
BssNI GRCGYC 3 cut(s) 195, 834, 1007
BssT1I CCWWGG 2 cut(s) 696, 801
Bst2UI CCWGG 1 cut(s) 875
Bst6I CTCTTC 1 cut(s) 989
BstACI GRCGYC 3 cut(s) 195, 834, 1007
BstBAI YACGTR 1 cut(s) 1198
BstC8I GCNNGC 3 cut(s) 704, 1005, 1107
BstDEI CTNAG 2 cut(s) 248, 978
BstEII GGTNACC 1 cut(s) 869
BstF5I GGATG 3 cut(s) 203, 258, 1150
BstFNI CGCG 1 cut(s) 566
BstH2I RGCGCY 3 cut(s) 837, 1010, 1082
BstHHI GCGC 4 cut(s) 568, 836, 1009, 1081
BstKTI GATC 6 cut(s) 153, 385, 428, 495, 712, 808
BstMAI GTCTC 3 cut(s) 616, 904, 1115
BstMBI GATC 6 cut(s) 150, 382, 425, 492, 709, 805
BstMCI CGRYCG 1 cut(s) 1095
BstMWI GCNNNNNNNGC 8 cut(s) 62, 363, 402, 542, 692, 1154, 1163, 1187
BstNI CCWGG 1 cut(s) 875
BstPAI GACNNNNGTC 1 cut(s) 1046
BstPI GGTNACC 1 cut(s) 869
BstSCI CCNGG 6 cut(s) 352, 421, 501, 514, 581, 873
BstSLI GKGCMC 1 cut(s) 790
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 3 cut(s) 2, 1015, 1169
BstV2I GAAGAC 1 cut(s) 940
BstX2I RGATCY 4 cut(s) 425, 492, 709, 805
BstYI RGATCY 4 cut(s) 425, 492, 709, 805
BstZI CGGCCG 1 cut(s) 1092
BsuRI GGCC 6 cut(s) 5, 345, 536, 545, 695, 1094
BtgZI GCGATG 2 cut(s) 493, 984
BtsCI GGATG 3 cut(s) 203, 258, 1150
BtsIMutI CAGTG 4 cut(s) 918, 951, 967, 1230
BveI ACCTGC 1 cut(s) 1216
Cac8I GCNNGC 3 cut(s) 704, 1005, 1107
CfoI GCGC 4 cut(s) 568, 836, 1009, 1081
Cfr10I RCCGGY 4 cut(s) 341, 1003, 1021, 1105
Cfr13I GGNCC 1 cut(s) 535
CseI GACGC 1 cut(s) 184
CsiI ACCWGGT 1 cut(s) 873
Csp6I GTAC 5 cut(s) 103, 112, 754, 844, 1173
CviAII CATG 1 cut(s) 199
CviQI GTAC 5 cut(s) 103, 112, 754, 844, 1173
DdeI CTNAG 2 cut(s) 248, 978
DinI GGCGCC 2 cut(s) 835, 1008
DpnI GATC 6 cut(s) 152, 384, 427, 494, 711, 807
DpnII GATC 6 cut(s) 150, 382, 425, 492, 709, 805
DrdI GACNNNNNNGTC 2 cut(s) 311, 854
DseDI GACNNNNNNGTC 2 cut(s) 311, 854
EaeI YGGCCR 2 cut(s) 343, 1092
EagI CGGCCG 1 cut(s) 1092
Eam1104I CTCTTC 1 cut(s) 989
EarI CTCTTC 1 cut(s) 989
EciI GGCGGA 4 cut(s) 52, 403, 487, 1181
Ecl136II GAGCTC 2 cut(s) 982, 1136
EclXI CGGCCG 1 cut(s) 1092
Eco130I CCWWGG 2 cut(s) 696, 801
Eco24I GRGCYC 2 cut(s) 984, 1138
Eco52I CGGCCG 1 cut(s) 1092
Eco53kI GAGCTC 2 cut(s) 982, 1136
Eco72I CACGTG 1 cut(s) 1198
Eco88I CYCGRG 2 cut(s) 98, 488
Eco91I GGTNACC 1 cut(s) 869
EcoICRI GAGCTC 2 cut(s) 982, 1136
EcoO65I GGTNACC 1 cut(s) 869
EcoRII CCWGG 1 cut(s) 873
EcoT14I CCWWGG 2 cut(s) 696, 801
EcoT38I GRGCYC 2 cut(s) 984, 1138
EgeI GGCGCC 2 cut(s) 835, 1008
EheI GGCGCC 2 cut(s) 835, 1008
ErhI CCWWGG 2 cut(s) 696, 801
Esp3I CGTCTC 2 cut(s) 616, 904
FaeI CATG 1 cut(s) 202
FaiI YATR 1 cut(s) 200
FaqI GGGAC 1 cut(s) 749
FatI CATG 1 cut(s) 198
FauI CCCGC 4 cut(s) 20, 167, 289, 611
FblI GTMKAC 1 cut(s) 957
FokI GGATG 3 cut(s) 190, 245, 1157
FriOI GRGCYC 2 cut(s) 984, 1138
FspBI CTAG 2 cut(s) 140, 572
GlaI GCGC 4 cut(s) 567, 835, 1008, 1080
GsaI CCCAGC 2 cut(s) 1062, 1067
GsuI CTGGAG 3 cut(s) 424, 598, 889
HaeII RGCGCY 3 cut(s) 837, 1010, 1082
HaeIII GGCC 6 cut(s) 5, 345, 536, 545, 695, 1094
HgaI GACGC 1 cut(s) 184
HhaI GCGC 4 cut(s) 568, 836, 1009, 1081
Hin1I GRCGYC 3 cut(s) 195, 834, 1007
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 4 cut(s) 566, 834, 1007, 1079
HinP1I GCGC 4 cut(s) 566, 834, 1007, 1079
HincII GTYRAC 2 cut(s) 373, 958
HindII GTYRAC 2 cut(s) 373, 958
HinfI GANTC 6 cut(s) 437, 466, 661, 894, 909, 929
Hpy166II GTNNAC 2 cut(s) 373, 958
Hpy188I TCNGA 5 cut(s) 304, 550, 714, 1120, 1144
Hpy8I GTNNAC 2 cut(s) 373, 958
HpyAV CCTTC 6 cut(s) 130, 272, 604, 1044, 1125, 1267
HpyCH4IV ACGT 1 cut(s) 1197
HpyCH4V TGCA 4 cut(s) 588, 751, 1148, 1211
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 363, 402, 542, 692, 1154, 1163, 1187
HpyF3I CTNAG 2 cut(s) 248, 978
HpySE526I ACGT 1 cut(s) 1197
Hsp92I GRCGYC 3 cut(s) 195, 834, 1007
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 4 cut(s) 566, 834, 1007, 1079
KasI GGCGCC 2 cut(s) 833, 1006
KroI GCCGGC 2 cut(s) 1003, 1105
KroNI GCCGGC 2 cut(s) 1005, 1107
Kzo9I GATC 6 cut(s) 150, 382, 425, 492, 709, 805
LguI GCTCTTC 1 cut(s) 989
Lsp1109I GCAGC 3 cut(s) 2, 1015, 1169
LweI GCATC 2 cut(s) 528, 1135
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 2 cut(s) 140, 572
MaeII ACGT 1 cut(s) 1197
MaeIII GTNAC 7 cut(s) 169, 856, 869, 877, 1193, 1198, 1243
MalI GATC 6 cut(s) 152, 384, 427, 494, 711, 807
MbiI CCGCTC 1 cut(s) 530
MboI GATC 6 cut(s) 150, 382, 425, 492, 709, 805
MboII GAAGA 3 cut(s) 940, 959, 976
MflI RGATCY 4 cut(s) 425, 492, 709, 805
MhlI GDGCHC 5 cut(s) 627, 790, 984, 1138, 1230
Mly113I GGCGCC 2 cut(s) 834, 1007
MlyI GAGTC 5 cut(s) 431, 655, 903, 918, 923
MmeI TCCRAC 2 cut(s) 327, 1122
MreI CGCCGGCG 1 cut(s) 1003
MroNI GCCGGC 2 cut(s) 1003, 1105
MseI TTAA 3 cut(s) 125, 605, 690
MspA1I CMGCKG 3 cut(s) 15, 618, 1160
MspR9I CCNGG 6 cut(s) 354, 423, 503, 516, 583, 875
MvaI CCWGG 1 cut(s) 875
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 8 cut(s) 62, 363, 402, 542, 692, 1154, 1163, 1187
NaeI GCCGGC 2 cut(s) 1005, 1107
NarI GGCGCC 2 cut(s) 834, 1007
NciI CCSGG 5 cut(s) 354, 423, 503, 516, 583
NdeII GATC 6 cut(s) 150, 382, 425, 492, 709, 805
NgoMIV GCCGGC 2 cut(s) 1003, 1105
NlaIII CATG 1 cut(s) 202
NmeAIII GCCGAG 3 cut(s) 342, 757, 1157
NmuCI GTSAC 7 cut(s) 169, 856, 869, 877, 1193, 1198, 1243
PaeR7I CTCGAG 1 cut(s) 488
PaqCI CACCTGC 1 cut(s) 1216
PciSI GCTCTTC 1 cut(s) 989
PcsI WCGNNNNNNNCGW 4 cut(s) 273, 852, 1007, 1148
PdiI GCCGGC 2 cut(s) 1005, 1107
PfeI GAWTC 1 cut(s) 466
PflFI GACNNNGTC 2 cut(s) 875, 920
PleI GAGTC 5 cut(s) 431, 655, 902, 917, 923
PluTI GGCGCC 2 cut(s) 837, 1010
PmaCI CACGTG 1 cut(s) 1198
PmlI CACGTG 1 cut(s) 1198
PpsI GAGTC 5 cut(s) 431, 655, 902, 917, 923
Ppu21I YACGTR 1 cut(s) 1198
PshAI GACNNNNGTC 1 cut(s) 1046
Psp124BI GAGCTC 2 cut(s) 984, 1138
Psp6I CCWGG 1 cut(s) 873
PspCI CACGTG 1 cut(s) 1198
PspEI GGTNACC 1 cut(s) 869
PspFI CCCAGC 2 cut(s) 1058, 1063
PspGI CCWGG 1 cut(s) 873
PspPI GGNCC 1 cut(s) 535
PsuI RGATCY 4 cut(s) 425, 492, 709, 805
PsyI GACNNNGTC 2 cut(s) 875, 920
RsaI GTAC 5 cut(s) 104, 113, 755, 845, 1174
RsaNI GTAC 5 cut(s) 103, 112, 754, 844, 1173
SacI GAGCTC 2 cut(s) 984, 1138
SalI GTCGAC 1 cut(s) 956
SapI GCTCTTC 1 cut(s) 989
SaqAI TTAA 3 cut(s) 125, 605, 690
Sau3AI GATC 6 cut(s) 150, 382, 425, 492, 709, 805
Sau96I GGNCC 1 cut(s) 535
SchI GAGTC 5 cut(s) 431, 655, 903, 918, 923
ScrFI CCNGG 6 cut(s) 354, 423, 503, 516, 583, 875
SduI GDGCHC 5 cut(s) 627, 790, 984, 1138, 1230
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 2 cut(s) 528, 1135
SfiI GGCCNNNNNGGCC 1 cut(s) 542
SfoI GGCGCC 2 cut(s) 835, 1008
Sfr274I CTCGAG 1 cut(s) 488
SgrAI CRCCGGYG 1 cut(s) 1003
SlaI CTCGAG 1 cut(s) 488
SmlI CTYRAG 3 cut(s) 488, 626, 791
SmoI CTYRAG 3 cut(s) 488, 626, 791
SspDI GGCGCC 2 cut(s) 833, 1006
SspMI CTAG 2 cut(s) 140, 572
SstI GAGCTC 2 cut(s) 984, 1138
StyD4I CCNGG 6 cut(s) 352, 421, 501, 514, 581, 873
StyI CCWWGG 2 cut(s) 696, 801
TaiI ACGT 1 cut(s) 1200
TaqI TCGA 7 cut(s) 87, 267, 435, 489, 927, 957, 1013
TatI WGTACW 2 cut(s) 102, 111
TauI GCSGC 8 cut(s) 53, 56, 215, 396, 419, 1097, 1163, 1166
TfiI GAWTC 1 cut(s) 466
Tru1I TTAA 3 cut(s) 125, 605, 690
Tru9I TTAA 3 cut(s) 125, 605, 690
TscAI CASTG 4 cut(s) 925, 958, 967, 1230
TseFI GTSAC 7 cut(s) 169, 856, 869, 877, 1193, 1198, 1243
TseI GCWGC 3 cut(s) 15, 1028, 1157
Tsp45I GTSAC 7 cut(s) 169, 856, 869, 877, 1193, 1198, 1243
TspGWI ACGGA 8 cut(s) 220, 301, 445, 472, 927, 1060, 1128, 1200
TspRI CASTG 4 cut(s) 925, 958, 967, 1230
Tth111I GACNNNGTC 2 cut(s) 875, 920
XbaI TCTAGA 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 957
XspI CTAG 2 cut(s) 140, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.