RchiOBHm_Chr2g0105331

Multidrug and

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016734
Physical Location & Seq
Reverse (-)
16640548 .. 16641930
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47955

Sequence Viewer

Length: 1383 bp
ATGATCTTATCCGGCCTCTTAAACTACTCAAAGGCAGCCATCTCCATGTTTTTCATGGGGAAGCTTGGGAAATCTGCTCTGGCAGGAGGAAGCCTCGCCATAGGTGTTGCAAACATCACCGGATACTCTTTCATCTCCGGTCTCGCTACAGGGATGGATGGAATCGCTTCACAAGCATTTGGTGCTCGTAACGGCTGTCTTCTGTCACACACTCTACAGAGGACAATAGTTCTCCTACTCATCCTTTGCATGCCCATCACTTTGTTGTGGTATAACTTCGAATCTGTCCTTATCCACTCTGGCCAAAACCCATCCATCTCATCCATAGCCATTACCTATCTCAGATTCTCCATCCCAACCCTCTTGTTTCAGTCCCTCATCCACCCCATTAAAATTTACCTAAGGAGCCAGAAAGTCACCAGCCCTTTTACGCTCAGCACTGCCTTTTCGCTAGCCGTTCACATCCCAGCCAACTACGTCGCCGTTTACTGTCTTGACCTTGGCCTGGAAGGGATTGCACTCTCCGGTTGCTTGGCCGACTTCAATATCATGATGTCTCTTCTGGTTTATCTCTTTTTTTCTGGCCATCTTACTGTGAATTCTCTGTGGTTCTGGAGAGACTGTGCGTCGTTTAGACACTATGAAGATTGGAGCCCCATTTTCAGTCTGGCGCTTCCGAGTTGCGCGTCGGTGTGTTTAGAGTGGTGGTGGTATGAGATCATGATTGTTTTCTCTGGACTTCTTGTCAATGCACCCGAGGCTGTGGCCACCATGGGAATACTGATCCAGATGACCGCATTGATCTATCAGTTTCCTATCTCGTTGAACCAGGCCGTGTCGACTAGGGTGGGGAACGAGTTGGGGGCCGACAGACCGAAGCAAGCTCTGATGGCGTCTGTAGTTGCACTGGCATGTGCTATTTTCACAGGCATTGTCGCCATGAGTTTCATGATCGGAATGCGCAACGTTTGGGGGGGAATGTTCACGTCTGATCAGGCCATTATATCGCTGGTGGCTTCGGTCTTGCCGGTGGTAGGGCTTTGTGAGCTCGGGAACTGCCCCCAGACCACCGTCTGTGGAGTTTTGAGAGGCTGTGCACGGCCAACTTTGGCTGCTTACATAAACTTCGGCTCGTTTTATGGTGTGGGATTGCCTGCGGCCCTTATGTTGGGCTTTGGGGTGGAGTTGGGCTTATTGGGCCTGTGGTTGGGCTTACTTGCGGCCCAAATGGTTTGCTTTGGGATCATGGTAGTGGCATTACTTCGAACGGACTGGGAGGAACAAGCAAATCGAGCATATGTACTCACATGTAGCTATCTAGGGGAGGAAGAAAATGAAACAAGCAAGGAAGAACCAGCACCTCCGGTTGTGGTTGTGAACTAG

Protein Analysis

460

Amino Acids

49.69

Weight (kDa)

5.69

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 2 - 160 3.3e-28 MatE
MatE PF01554 226 - 387 2.1e-29 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016734)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15940 FvH4_1g15940
malus_domestica MD15G1282700.v1.1
prunus_persica Prupe.7G134400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105331
rosa_laevigata RLG00000017417
rosa_multiflora Rmu_sc0001407.1_g000017
rosa_roxburghii Rroxscaffold_2G00138280
rosa_rugosa Rorug02G0127700
rosa_samantha Rh2AG178300 Rh2BG186800 Rh2CG182900 Rh2DG184300
rosa_wichuraiana Rw2G014000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 962
AccI GTMKAC 1 cut(s) 839
AccII CGCG 1 cut(s) 686
AciI CCGC 3 cut(s) 795, 1157, 1220
AclI AACGTT 1 cut(s) 966
AclWI GGATC 2 cut(s) 778, 1250
AcoI YGGCCR 5 cut(s) 301, 534, 583, 765, 1100
AcsI RAATTY 2 cut(s) 393, 598
AcyI GRCGYC 1 cut(s) 893
AfaI GTAC 1 cut(s) 1302
AfiI CCNNNNNNNGG 4 cut(s) 505, 1034, 1168, 1207
AflIII ACRYGT 1 cut(s) 1307
AgsI TTSAA 2 cut(s) 544, 826
AhdI GACNNNNNGTC 2 cut(s) 625, 743
AjiI CACGTC 1 cut(s) 987
AjnI CCWGG 2 cut(s) 504, 828
AluBI AGCT 4 cut(s) 64, 884, 1048, 1314
AluI AGCT 4 cut(s) 64, 884, 1048, 1314
Alw21I GWGCWC 3 cut(s) 187, 1050, 1099
Alw26I GTCTC 3 cut(s) 146, 561, 612
Alw44I GTGCAC 1 cut(s) 1095
AlwI GGATC 2 cut(s) 778, 1250
Ama87I CYCGRG 2 cut(s) 755, 1049
ApaLI GTGCAC 1 cut(s) 1095
ApeKI GCWGC 2 cut(s) 35, 1112
ApoI RAATTY 2 cut(s) 393, 598
Asp700I GAANNNNTTC 1 cut(s) 166
AspLEI GCGC 3 cut(s) 673, 686, 963
AspS9I GGNCC 4 cut(s) 864, 1159, 1198, 1222
AsuHPI GGTGA 2 cut(s) 109, 409
AsuII TTCGAA 2 cut(s) 279, 1264
AsuNHI GCTAGC 1 cut(s) 451
AvaI CYCGRG 2 cut(s) 755, 1049
AxyI CCTNAGG 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 1099
BalI TGGCCA 3 cut(s) 303, 585, 767
BanII GRGCYC 2 cut(s) 656, 1050
BbsI GAAGAC 1 cut(s) 191
Bbv12I GWGCWC 3 cut(s) 187, 1050, 1099
BbvI GCAGC 2 cut(s) 47, 1099
BccI CCATC 9 cut(s) 47, 148, 152, 263, 319, 323, 359, 594, 883
BceAI ACGGC 5 cut(s) 208, 440, 467, 818, 1115
BciT130I CCWGG 2 cut(s) 506, 830
BciVI GTATCC 1 cut(s) 116
BclI TGATCA 1 cut(s) 991
BcoDI GTCTC 3 cut(s) 146, 561, 612
BfaI CTAG 4 cut(s) 452, 843, 1319, 1381
BfmI CTRYAG 3 cut(s) 147, 215, 897
BfoI RGCGCY 1 cut(s) 674
BfuI GTATCC 1 cut(s) 116
BisI GCNGC 4 cut(s) 36, 1113, 1158, 1221
BlpI GCTNAGC 1 cut(s) 434
BlsI GCNGC 4 cut(s) 37, 1114, 1159, 1222
Bme1390I CCNGG 2 cut(s) 506, 830
BmeRI GACNNNNNGTC 2 cut(s) 625, 743
BmeT110I CYCGRG 2 cut(s) 755, 1049
BmgBI CACGTC 1 cut(s) 987
BmgT120I GGNCC 4 cut(s) 864, 1159, 1198, 1222
BmiI GGNNCC 3 cut(s) 407, 653, 865
BmrFI CCNGG 2 cut(s) 506, 830
BmrI ACTGGG 1 cut(s) 1282
BmtI GCTAGC 1 cut(s) 455
BmuI ACTGGG 1 cut(s) 1282
BoxI GACNNNNGTC 1 cut(s) 1070
BpiI GAAGAC 1 cut(s) 191
BplI GAGNNNNNCTC 2 cut(s) 78, 110
BpmI CTGGAG 1 cut(s) 634
Bpu1102I GCTNAGC 1 cut(s) 434
Bpu14I TTCGAA 2 cut(s) 279, 1264
BsaHI GRCGYC 1 cut(s) 893
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 499, 756, 771
BsaWI WCCGGW 4 cut(s) 119, 137, 524, 1363
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bsc4I CCNNNNNNNGG 4 cut(s) 505, 1034, 1168, 1207
Bse118I RCCGGY 1 cut(s) 1027
Bse1I ACTGG 2 cut(s) 912, 1277
Bse21I CCTNAGG 1 cut(s) 401
BseBI CCWGG 2 cut(s) 506, 830
BseDI CCNNGG 3 cut(s) 499, 756, 771
BseGI GGATG 8 cut(s) 159, 163, 240, 311, 320, 351, 378, 462
BseLI CCNNNNNNNGG 4 cut(s) 505, 1034, 1168, 1207
BseMII CTCAG 2 cut(s) 355, 448
BseNI ACTGG 2 cut(s) 912, 1277
BseSI GKGCMC 1 cut(s) 1099
BseXI GCAGC 2 cut(s) 47, 1099
BseYI CCCAGC 1 cut(s) 466
Bsh1236I CGCG 1 cut(s) 686
BsiHKAI GWGCWC 3 cut(s) 187, 1050, 1099
BsiHKCI CYCGRG 2 cut(s) 755, 1049
BsiSI CCGG 6 cut(s) 12, 120, 138, 525, 1028, 1364
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 4 cut(s) 505, 1034, 1168, 1207
BsmAI GTCTC 3 cut(s) 146, 561, 612
BsmFI GGGAC 1 cut(s) 358
BsmI GAATGC 1 cut(s) 963
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 2 cut(s) 755, 1049
Bsp119I TTCGAA 2 cut(s) 279, 1264
Bsp1286I GDGCHC 4 cut(s) 187, 656, 1050, 1099
Bsp143I GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
Bsp1720I GCTNAGC 1 cut(s) 434
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 3 cut(s) 795, 1157, 1220
BspCNI CTCAG 2 cut(s) 354, 447
BspFNI CGCG 1 cut(s) 686
BspHI TCATGA 3 cut(s) 549, 720, 948
BspLI GGNNCC 3 cut(s) 407, 653, 865
BspOI GCTAGC 1 cut(s) 455
BspPI GGATC 2 cut(s) 778, 1250
BspT104I TTCGAA 2 cut(s) 279, 1264
BspTNI GGTCTC 1 cut(s) 146
BsrFI RCCGGY 1 cut(s) 1027
BsrI ACTGG 2 cut(s) 912, 1277
BssAI RCCGGY 1 cut(s) 1027
BssECI CCNNGG 3 cut(s) 499, 756, 771
BssMI GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
BssNI GRCGYC 1 cut(s) 893
BssT1I CCWWGG 2 cut(s) 499, 771
Bst2UI CCWGG 2 cut(s) 506, 830
Bst4CI ACNGT 4 cut(s) 491, 595, 623, 1072
Bst6I CTCTTC 1 cut(s) 564
BstACI GRCGYC 1 cut(s) 893
BstAPI GCANNNNNTGC 1 cut(s) 182
BstBI TTCGAA 2 cut(s) 279, 1264
BstC8I GCNNGC 4 cut(s) 251, 453, 882, 1155
BstDEI CTNAG 3 cut(s) 341, 401, 434
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 8 cut(s) 159, 163, 240, 311, 320, 351, 378, 462
BstFNI CGCG 1 cut(s) 686
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 3 cut(s) 673, 686, 963
BstKTI GATC 7 cut(s) 6, 720, 786, 804, 954, 994, 1245
BstMAI GTCTC 3 cut(s) 146, 561, 612
BstMBI GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
BstMWI GCNNNNNNNGC 7 cut(s) 173, 182, 758, 890, 1045, 1197, 1292
BstNI CCWGG 2 cut(s) 506, 830
BstNSI RCATGY 3 cut(s) 253, 915, 1311
BstPAI GACNNNNGTC 1 cut(s) 1070
BstSCI CCNGG 2 cut(s) 504, 828
BstSFI CTRYAG 3 cut(s) 147, 215, 897
BstSLI GKGCMC 1 cut(s) 1099
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 2 cut(s) 47, 1099
BstV2I GAAGAC 1 cut(s) 191
Bsu36I CCTNAGG 1 cut(s) 401
BsuI GTATCC 1 cut(s) 116
BtgI CCRYGG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 987
BtsCI GGATG 8 cut(s) 159, 163, 240, 311, 320, 351, 378, 462
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 2 cut(s) 438, 905
Cac8I GCNNGC 4 cut(s) 251, 453, 882, 1155
CciI TCATGA 3 cut(s) 549, 720, 948
CfoI GCGC 3 cut(s) 673, 686, 963
Cfr10I RCCGGY 1 cut(s) 1027
Cfr13I GGNCC 4 cut(s) 864, 1159, 1198, 1222
CseI GACGC 3 cut(s) 615, 675, 882
Csp6I GTAC 1 cut(s) 1301
CviQI GTAC 1 cut(s) 1301
DdeI CTNAG 3 cut(s) 341, 401, 434
DpnI GATC 7 cut(s) 5, 719, 785, 803, 953, 993, 1244
DpnII GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
DriI GACNNNNNGTC 2 cut(s) 625, 743
EaeI YGGCCR 5 cut(s) 301, 534, 583, 765, 1100
Eam1104I CTCTTC 1 cut(s) 564
Eam1105I GACNNNNNGTC 2 cut(s) 625, 743
EarI CTCTTC 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 1048
Eco130I CCWWGG 2 cut(s) 499, 771
Eco24I GRGCYC 2 cut(s) 656, 1050
Eco31I GGTCTC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 1048
Eco81I CCTNAGG 1 cut(s) 401
Eco88I CYCGRG 2 cut(s) 755, 1049
EcoICRI GAGCTC 1 cut(s) 1048
EcoRI GAATTC 1 cut(s) 598
EcoRII CCWGG 2 cut(s) 504, 828
EcoT14I CCWWGG 2 cut(s) 499, 771
EcoT38I GRGCYC 2 cut(s) 656, 1050
ErhI CCWWGG 2 cut(s) 499, 771
FaqI GGGAC 1 cut(s) 358
FauNDI CATATG 1 cut(s) 1297
FbaI TGATCA 1 cut(s) 991
FblI GTMKAC 1 cut(s) 839
Fnu4HI GCNGC 4 cut(s) 36, 1113, 1158, 1221
FokI GGATG 8 cut(s) 166, 170, 227, 298, 307, 338, 365, 449
FriOI GRGCYC 2 cut(s) 656, 1050
Fsp4HI GCNGC 4 cut(s) 36, 1113, 1158, 1221
FspBI CTAG 4 cut(s) 452, 843, 1319, 1381
FspI TGCGCA 1 cut(s) 962
GlaI GCGC 3 cut(s) 672, 685, 962
GluI GCNGC 4 cut(s) 36, 1113, 1158, 1221
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 1 cut(s) 674
HapII CCGG 6 cut(s) 12, 120, 138, 525, 1028, 1364
HgaI GACGC 3 cut(s) 615, 675, 882
HhaI GCGC 3 cut(s) 673, 686, 963
Hin1I GRCGYC 1 cut(s) 893
Hin6I GCGC 3 cut(s) 671, 684, 961
HinP1I GCGC 3 cut(s) 671, 684, 961
HincII GTYRAC 1 cut(s) 840
HindII GTYRAC 1 cut(s) 840
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 3 cut(s) 162, 281, 345
HpaII CCGG 6 cut(s) 12, 120, 138, 525, 1028, 1364
HphI GGTGA 2 cut(s) 109, 409
Hpy166II GTNNAC 6 cut(s) 460, 487, 840, 984, 1097, 1378
Hpy188I TCNGA 5 cut(s) 344, 678, 888, 956, 991
Hpy188III TCNNGA 8 cut(s) 494, 550, 613, 721, 735, 787, 949, 1051
Hpy8I GTNNAC 6 cut(s) 460, 487, 840, 984, 1097, 1378
Hpy99I CGWCG 3 cut(s) 482, 631, 691
HpyAV CCTTC 1 cut(s) 503
HpyCH4III ACNGT 4 cut(s) 491, 595, 623, 1072
HpyCH4IV ACGT 3 cut(s) 477, 966, 986
HpyCH4V TGCA 6 cut(s) 110, 249, 518, 752, 905, 1097
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 182, 758, 890, 1045, 1197, 1292
HpyF3I CTNAG 3 cut(s) 341, 401, 434
HpySE526I ACGT 3 cut(s) 477, 966, 986
Hsp92I GRCGYC 1 cut(s) 893
HspAI GCGC 3 cut(s) 671, 684, 961
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
LmnI GCTCC 2 cut(s) 405, 651
Lsp1109I GCAGC 2 cut(s) 47, 1099
MaeI CTAG 4 cut(s) 452, 843, 1319, 1381
MaeII ACGT 3 cut(s) 477, 966, 986
MaeIII GTNAC 3 cut(s) 188, 204, 415
MalI GATC 7 cut(s) 5, 719, 785, 803, 953, 993, 1244
MboI GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
MboII GAAGA 5 cut(s) 191, 551, 656, 1340, 1361
MhlI GDGCHC 4 cut(s) 187, 656, 1050, 1099
MlsI TGGCCA 3 cut(s) 303, 585, 767
MluCI AATT 2 cut(s) 393, 598
MluNI TGGCCA 3 cut(s) 303, 585, 767
Mox20I TGGCCA 3 cut(s) 303, 585, 767
MroXI GAANNNNTTC 1 cut(s) 166
MscI TGGCCA 3 cut(s) 303, 585, 767
MseI TTAA 2 cut(s) 20, 390
MslI CAYNNNNRTG 3 cut(s) 44, 910, 1250
Msp20I TGGCCA 3 cut(s) 303, 585, 767
MspI CCGG 6 cut(s) 12, 120, 138, 525, 1028, 1364
MspR9I CCNGG 2 cut(s) 506, 830
Mva1269I GAATGC 1 cut(s) 963
MvaI CCWGG 2 cut(s) 506, 830
MvnI CGCG 1 cut(s) 686
MwoI GCNNNNNNNGC 7 cut(s) 173, 182, 758, 890, 1045, 1197, 1292
NcoI CCATGG 1 cut(s) 771
NdeI CATATG 1 cut(s) 1297
NdeII GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
NheI GCTAGC 1 cut(s) 451
NlaIV GGNNCC 3 cut(s) 407, 653, 865
NmuCI GTSAC 2 cut(s) 204, 415
NsbI TGCGCA 1 cut(s) 962
NspI RCATGY 3 cut(s) 253, 915, 1311
NspV TTCGAA 2 cut(s) 279, 1264
PaeI GCATGC 1 cut(s) 253
PagI TCATGA 3 cut(s) 549, 720, 948
PciI ACATGT 1 cut(s) 1307
PctI GAATGC 1 cut(s) 963
PdmI GAANNNNTTC 1 cut(s) 166
PfeI GAWTC 3 cut(s) 162, 281, 345
PkrI GCNGC 4 cut(s) 37, 1114, 1159, 1222
PscI ACATGT 1 cut(s) 1307
PshAI GACNNNNGTC 1 cut(s) 1070
Psp124BI GAGCTC 1 cut(s) 1050
Psp1406I AACGTT 1 cut(s) 966
Psp6I CCWGG 2 cut(s) 504, 828
PspFI CCCAGC 1 cut(s) 466
PspGI CCWGG 2 cut(s) 504, 828
PspN4I GGNNCC 3 cut(s) 407, 653, 865
PspPI GGNCC 4 cut(s) 864, 1159, 1198, 1222
RsaI GTAC 1 cut(s) 1302
RsaNI GTAC 1 cut(s) 1301
RseI CAYNNNNRTG 3 cut(s) 44, 910, 1250
SacI GAGCTC 1 cut(s) 1050
SalI GTCGAC 1 cut(s) 838
SaqAI TTAA 2 cut(s) 20, 390
SatI GCNGC 4 cut(s) 36, 1113, 1158, 1221
Sau3AI GATC 7 cut(s) 3, 717, 783, 801, 951, 991, 1242
Sau96I GGNCC 4 cut(s) 864, 1159, 1198, 1222
ScrFI CCNGG 2 cut(s) 506, 830
SduI GDGCHC 4 cut(s) 187, 656, 1050, 1099
SfcI CTRYAG 3 cut(s) 147, 215, 897
SfuI TTCGAA 2 cut(s) 279, 1264
SmiMI CAYNNNNRTG 3 cut(s) 44, 910, 1250
SphI GCATGC 1 cut(s) 253
Sse9I AATT 2 cut(s) 393, 598
SsiI CCGC 3 cut(s) 795, 1157, 1220
SspMI CTAG 4 cut(s) 452, 843, 1319, 1381
SstI GAGCTC 1 cut(s) 1050
StyD4I CCNGG 2 cut(s) 504, 828
StyI CCWWGG 2 cut(s) 499, 771
TaaI ACNGT 4 cut(s) 491, 595, 623, 1072
TaiI ACGT 3 cut(s) 480, 969, 989
TaqI TCGA 4 cut(s) 279, 839, 1264, 1291
TaqII GACCGA 2 cut(s) 889, 1009
TasI AATT 2 cut(s) 393, 598
TatI WGTACW 1 cut(s) 1300
TauI GCSGC 2 cut(s) 1160, 1223
TfiI GAWTC 3 cut(s) 162, 281, 345
Tru1I TTAA 2 cut(s) 20, 390
Tru9I TTAA 2 cut(s) 20, 390
TscAI CASTG 2 cut(s) 445, 912
TseFI GTSAC 2 cut(s) 204, 415
TseI GCWGC 2 cut(s) 35, 1112
Tsp45I GTSAC 2 cut(s) 204, 415
TspDTI ATGAA 5 cut(s) 43, 121, 657, 937, 1350
TspGWI ACGGA 1 cut(s) 1283
TspRI CASTG 2 cut(s) 445, 912
VneI GTGCAC 1 cut(s) 1095
XapI RAATTY 2 cut(s) 393, 598
XceI RCATGY 3 cut(s) 253, 915, 1311
XcmI CCANNNNNNNNNTGG 3 cut(s) 52, 664, 1006
XmiI GTMKAC 1 cut(s) 839
XmnI GAANNNNTTC 1 cut(s) 166
XspI CTAG 4 cut(s) 452, 843, 1319, 1381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.