RchiOBHm_Chr2g0142201

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0022652
Physical Location & Seq
Forward (+)
59661226 .. 59668663
7438 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51238

Sequence Viewer

Length: 2967 bp
ATGGCTGAAACACTCATTAATCTACTCCTGGAGCGTTTGGCTTCGGTCACCGTTAACAGGATCGGAAGAGAGCTGCAGCTGGTGATAGGTGTTGATGATGAAGTCGCAAACCTGTCCCGCAATTTGAGACTCATCACAAATGTGCTTCAGGATGCGGAGAGACAAGTCACGGAGGCCAGCATGGGAGAAGTGACGGGGGCCAGCGTGGGAGATTGGCTGAATGAACTGAACCAAGTTTCTCATGAGATGGACGACGTGTTGGATGAGTGGTGCACAGAAATTCTTAAGCATGAACAAGAAGGTGTGAACCCAACGGAGGCCTTGGAGATTCCAGAGGGTTCAAACAAAGGGGGAATTATTGAAACTGCTCCTCGTACTAAGGAGAAGGTATGTCTCCCCCTTCCTTCTTGCTTTTCTTGCTGCCGAGTCAAACAAGTAGTTCGTCTTCCAGTCAATATAGTCCGTCGAGTAGTTCATCGTCGCGACATTGCGGTCAAAATACAGAGTCTAAACGAAAAGTTAACTCGGATTGCTAATGAAAGACAACGTTATAACTTTCAAGATACAAAAGAAGCACCTAAACCACCTCCACGACAACTAACTTCTTCTCTTGTCATTGACAAATCCTTTGGTCGAAAAACCGAAAAGGACCTTTTAGTAGGCAAGTTGCTAGATGAGAGTAGTAGTCATAAGAGGGGGGGTCCTCTTATCATCCCTATAATAGGATTGGGAGGGATAGGGAGAACAACTCTTTGTCAACTAGTCTATAATGATGACATGGTCAACACCTATTTTGACAAGAAGATATGGGTCTGTGTCTCAGATCCTTTTGAAGAGATTAAGATTGCTAAAGCCATCATAGAAGGTCTTGATAATGTGAACAATACTCCAAACTCAAATCAGTTAGACCCTTTTATGGTACGCATAAAAGAATTAATCAAGGGAAAAAAATTTCTCCTCGTCCTAGATGATGTGTGGAACCCAAATTCTAGTCAATGGAATGAATTAATCAAACCCTTGCTTGAGGGTGCCACTGGCAGTAGAATATTGGTAACCACACGAAAAGAGGAGGTTGCTCAAGTAATGGGAGCGACCAATCAAATGATTCACTTGAAGGCATTAAGTGAAGAGTGCACTCGGGAGCTGTTCAATCACATTACCTTCCTTGATAGGGAAGATGAGTTTGAAAAGTTTGAAATGTTTGTCCAAGAGTTTGTAGAGAAGTGTAAAGGTTTACCTCTTGTTGCGAAGACTTTAGGTAGTCTCATGCGCCATAAGAAAACAAAAAAAGAATGGCAAGATGTATTACAAAGTCCAATATGGGAATTAAAAAAAATTCCGGAGGAGGTTTTTCAACCATTATTGTTAAGTTATTATGATTTGTCCCCAACCATTAGACGTTGTCTTTTGTATTGCGTTACTTTTCCGAAAGATCATGTGATTGATAAAGAAGTTTTGATTGAGTTGTGGATGTCACAAGACTATTTGTACAAGAAGCAAAAGAAAGAAGACGAGGTTGGTCAAACATATTTTGAGGAGTTAGCAATGAGGTCTTTCTTCCAAGATTTTGAGAGAGACGATGATGGGAATGTTACAAAATGCAAAATGCACGATATTATGCATGACCTTTTGCAGTTCTTGACCAAGGAAGAATGTTTTAGTATGGAGGTGAAGGGTGCTGATGAGATGTTGGAGCTTCCCAGCAGTAACAGTTTTCGTCACTTCACGCTGATGTTTGCATCTCAGGGTTTATCTAGCATCCTTTCTTCTCAAAATTGTGGAAATTTGCGTACCTTCACTACTTTTGCTTCAACAATTTATAGTATTGAGCCAGAGTTTATAACTTCACTGAAACGTGTTAGGACTTTAAATTTGAGTAGTACTGCCCTCGGAGTAGTTCCAAAAGAGGTTGGTGAATTGCTACATTTGAGGTATCTTGATCTATCAGATAATGGCTTTATGGAATTACCTGACAAAATAGGTGATTTGTACAATCTACAAACCTTGCGGCTTGTTCGGTGCTATAATCTGCTAAAAGGGCCTGATCTGAGGAAGTTAATCAATTTGAAGCATCTTTACGTCCAGGGTAGTCTGCGTTTAGAGTCAAAAGGTATTAGTAGATTGAAATCGCTGCAAACACTAGATGAGTTTCACGTGAGCGAAGATACCAGAGAAGAAGGATTGTTGGAGGATCTGGAAAACTTGGACAAGCTTCAAGGGGCTCTTTCCATGCAACACTTGGCATCCCCATTGCAAACTGTGAGTGGGGCCAAGAGAGCACGGTTGAATACGAAACGCCTCCTCCACTTGGAACTTAATTTCCGCTGTGACTGTAGAAGAAGAATACGGAGGGGCGAAGCCGAAGGAGCGATACTGAATGCTTTAAAACCACATCCAGATTTGGAATCATTAAGCATCCTTGGGTGTCGTGGGGCTACCTCGACTCCCACTTGGCTCATGTCTTTACCAAACTTGAAGAGGCTTACTCTTTACATCAGGTATTTGGAGGATTTACCTGCTTTAGGGACACTGTCGTTCCTTGAATCGCTGGATATAAAGATGACTGATAAACTGCAAAAGGTGGGTGTTGAGTTTCTGGGGATAAATCGAATATCATCATCGTCGTCATCGGAGGTTATTTCATTCCCAAAGTTAAAACACCTCACATTCAGTTTCATGGATGAATGGAAAGAGTGGGAAGAAGTGGATTCTCAGAATATCAGGATAATGCCATGCCTTTCATCCTTAGAAGTGAGGAACTGTCGGAAACTAAAAGCTCTGCCGGAGTTCCTATGGAAGACACCACTGAAGAGTTTGACCATTACGAGTTGTCCAATTCTGCATTCTGTGTACTACGGAAGCGAACGTTGTCATTGGACCACTTATGGTGAGAAGCCAGCCCCGATTAATGAGGGGTTTAACAAGATATCTCACATCCCAGAAATCAGAATAGGTTATGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

988

Amino Acids

113.09

Weight (kDa)

6.24

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 100 1.3e-15 Rx N-terminal domain
NB-ARC PF00931 216 - 388 7e-33 NB-ARC domain
WHD_DRP PF23559 475 - 543 7.8e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 596 - 919 4.8e-29 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 729 - 853 2.7e-20 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022652)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0142201
rosa_multiflora Rmu_sc0001211.1_g000061
rosa_roxburghii Rroxscaffold_2G00103610
rosa_rugosa Rorug02G0371100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 552, 1841
AasI GACNNNNNNGTC 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 2524
AccB1I GGYRCC 1 cut(s) 1028
AccII CGCG 1 cut(s) 483
AccIII TCCGGA 1 cut(s) 1339
AciI CCGC 5 cut(s) 118, 155, 491, 2008, 2323
AclI AACGTT 2 cut(s) 547, 2866
AclWI GGATC 3 cut(s) 68, 818, 2199
AcsI RAATTY 7 cut(s) 279, 950, 985, 1335, 1783, 1870, 2960
AcuI CTGAAG 2 cut(s) 131, 2828
AcvI CACGTG 1 cut(s) 2155
AfaI GTAC 7 cut(s) 376, 921, 1490, 1792, 1882, 1991, 2852
AfiI CCNNNNNNNGG 7 cut(s) 57, 316, 722, 916, 1171, 2308, 2522
AflII CTTAAG 1 cut(s) 284
AflIII ACRYGT 2 cut(s) 255, 1855
AhlI ACTAGT 1 cut(s) 760
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 2 cut(s) 27, 2082
AjuI GAANNNNNNNTTGG 4 cut(s) 1500, 1532, 2827, 2859
AleI CACNNNNGTG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 2304, 2336
AluBI AGCT 6 cut(s) 73, 79, 1144, 1696, 2212, 2777
AluI AGCT 6 cut(s) 73, 79, 1144, 1696, 2212, 2777
Alw21I GWGCWC 3 cut(s) 275, 1136, 2281
Alw26I GTCTC 6 cut(s) 121, 154, 398, 823, 1268, 1569
Alw44I GTGCAC 2 cut(s) 271, 1132
AlwI GGATC 3 cut(s) 68, 818, 2199
Ama87I CYCGRG 1 cut(s) 1137
Aor13HI TCCGGA 1 cut(s) 1339
AoxI GGCC 5 cut(s) 174, 198, 318, 2039, 2268
ApaLI GTGCAC 2 cut(s) 271, 1132
ApeKI GCWGC 4 cut(s) 73, 76, 420, 2131
ApoI RAATTY 7 cut(s) 279, 950, 985, 1335, 1783, 1870, 2960
ArsI GACNNNNNNTTYG 4 cut(s) 611, 643, 1855, 1887
AseI ATTAAT 4 cut(s) 18, 935, 1007, 2907
AspLEI GCGC 1 cut(s) 1272
AspS9I GGNCC 6 cut(s) 198, 649, 701, 2039, 2268, 2877
AsuHPI GGTGA 6 cut(s) 40, 94, 1681, 1925, 1994, 2900
AvaI CYCGRG 1 cut(s) 1137
AvaII GGWCC 3 cut(s) 649, 701, 2877
BaeGI GKGCMC 2 cut(s) 275, 1136
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 2224
BarI GAAGNNNNNNTAC 6 cut(s) 429, 461, 2060, 2092, 2355, 2387
BbrPI CACGTG 1 cut(s) 2155
BbsI GAAGAC 4 cut(s) 437, 1256, 1515, 2804
Bbv12I GWGCWC 3 cut(s) 275, 1136, 2281
BbvI GCAGC 4 cut(s) 60, 88, 407, 2118
BccI CCATC 3 cut(s) 241, 863, 1577
BciT130I CCWGG 2 cut(s) 29, 2084
BcoDI GTCTC 6 cut(s) 121, 154, 398, 823, 1268, 1569
BcuI ACTAGT 1 cut(s) 760
BfaI CTAG 6 cut(s) 671, 761, 965, 990, 1755, 2141
BfmI CTRYAG 2 cut(s) 74, 2332
BfrI CTTAAG 1 cut(s) 284
BfuAI ACCTGC 1 cut(s) 2524
BisI GCNGC 5 cut(s) 74, 77, 421, 2009, 2132
BlsI GCNGC 5 cut(s) 75, 78, 422, 2010, 2133
BmcAI AGTACT 1 cut(s) 1882
Bme1390I CCNGG 2 cut(s) 29, 2084
Bme18I GGWCC 3 cut(s) 649, 701, 2877
BmeT110I CYCGRG 1 cut(s) 1137
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 6 cut(s) 198, 649, 701, 2039, 2268, 2877
BmiI GGNNCC 5 cut(s) 199, 702, 980, 1030, 2269
BmrFI CCNGG 2 cut(s) 29, 2084
BmsI GCATC 6 cut(s) 142, 1748, 1767, 2080, 2252, 2424
BpiI GAAGAC 4 cut(s) 437, 1256, 1515, 2804
BplI GAGNNNNNCTC 4 cut(s) 733, 765, 2470, 2502
BpmI CTGGAG 1 cut(s) 50
BpuEI CTTGAG 2 cut(s) 1043, 1062
BsaAI YACGTR 1 cut(s) 2155
BsaBI GATNNNNATC 1 cut(s) 2125
BsaJI CCNNGG 5 cut(s) 321, 1644, 1888, 2083, 2419
BsaWI WCCGGW 1 cut(s) 1339
BsaXI ACNNNNNCTCC 4 cut(s) 2286, 2316, 2428, 2458
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 316, 722, 916, 1171, 2308, 2522
Bse1I ACTGG 2 cut(s) 449, 1039
Bse3DI GCAATG 3 cut(s) 486, 1551, 2249
Bse8I GATNNNNATC 1 cut(s) 2125
BseAI TCCGGA 1 cut(s) 1339
BseBI CCWGG 2 cut(s) 29, 2084
BseDI CCNNGG 5 cut(s) 321, 1644, 1888, 2083, 2419
BseJI GATNNNNATC 1 cut(s) 2125
BseLI CCNNNNNNNGG 7 cut(s) 57, 316, 722, 916, 1171, 2308, 2522
BseMI GCAATG 3 cut(s) 486, 1551, 2249
BseMII CTCAG 4 cut(s) 834, 1757, 2039, 2726
BseNI ACTGG 2 cut(s) 449, 1039
BseRI GAGGAG 6 cut(s) 360, 947, 1082, 1358, 1550, 2291
BseSI GKGCMC 2 cut(s) 275, 1136
BseXI GCAGC 4 cut(s) 60, 88, 407, 2118
BseYI CCCAGC 1 cut(s) 1700
Bsh1236I CGCG 1 cut(s) 483
BshFI GGCC 5 cut(s) 176, 200, 320, 2041, 2270
BshNI GGYRCC 1 cut(s) 1028
BsiHKAI GWGCWC 3 cut(s) 275, 1136, 2281
BsiHKCI CYCGRG 1 cut(s) 1137
BsiSI CCGG 2 cut(s) 1340, 2783
BslFI GGGAC 3 cut(s) 100, 1369, 2539
BslI CCNNNNNNNGG 7 cut(s) 57, 316, 722, 916, 1171, 2308, 2522
BsmAI GTCTC 6 cut(s) 121, 154, 398, 823, 1268, 1569
BsmBI CGTCTC 1 cut(s) 1569
BsmFI GGGAC 3 cut(s) 100, 1369, 2539
BsmI GAATGC 2 cut(s) 2383, 2842
BsnI GGCC 5 cut(s) 176, 200, 320, 2041, 2270
BsoBI CYCGRG 1 cut(s) 1137
Bsp1286I GDGCHC 4 cut(s) 275, 1136, 2224, 2281
Bsp13I TCCGGA 1 cut(s) 1339
Bsp1407I TGTACA 2 cut(s) 1488, 1989
Bsp143I GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
Bsp68I TCGCGA 1 cut(s) 483
BspACI CCGC 5 cut(s) 118, 155, 491, 2008, 2323
BspANI GGCC 5 cut(s) 176, 200, 320, 2041, 2270
BspCNI CTCAG 4 cut(s) 833, 1756, 2040, 2725
BspEI TCCGGA 1 cut(s) 1339
BspFNI CGCG 1 cut(s) 483
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 5 cut(s) 199, 702, 980, 1030, 2269
BspMAI CTGCAG 1 cut(s) 78
BspMI ACCTGC 1 cut(s) 2524
BspPI GGATC 3 cut(s) 68, 818, 2199
BspT107I GGYRCC 1 cut(s) 1028
BspTI CTTAAG 1 cut(s) 284
BsrDI GCAATG 3 cut(s) 486, 1551, 2249
BsrGI TGTACA 2 cut(s) 1488, 1989
BsrI ACTGG 2 cut(s) 449, 1039
BssECI CCNNGG 5 cut(s) 321, 1644, 1888, 2083, 2419
BssMI GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
BssT1I CCWWGG 3 cut(s) 321, 1644, 2419
Bst2UI CCWGG 2 cut(s) 29, 2084
Bst4CI ACNGT 7 cut(s) 52, 1712, 2260, 2283, 2333, 2532, 2762
Bst6I CTCTTC 5 cut(s) 61, 828, 1122, 2471, 2804
BstAFI CTTAAG 1 cut(s) 284
BstAUI TGTACA 2 cut(s) 1488, 1989
BstBAI YACGTR 1 cut(s) 2155
BstC8I GCNNGC 3 cut(s) 178, 202, 2898
BstDEI CTNAG 6 cut(s) 378, 820, 1743, 2048, 2712, 2746
BstEII GGTNACC 2 cut(s) 46, 1051
BstENI CCTNNNNNAGG 2 cut(s) 720, 2520
BstFNI CGCG 1 cut(s) 483
BstHHI GCGC 1 cut(s) 1272
BstKTI GATC 6 cut(s) 63, 826, 1435, 1942, 2047, 2194
BstMAI GTCTC 6 cut(s) 121, 154, 398, 823, 1268, 1569
BstMBI GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
BstMWI GCNNNNNNNGC 4 cut(s) 417, 2038, 2276, 2366
BstNI CCWGG 2 cut(s) 29, 2084
BstPI GGTNACC 2 cut(s) 46, 1051
BstSCI CCNGG 2 cut(s) 27, 2082
BstSFI CTRYAG 2 cut(s) 74, 2332
BstSLI GKGCMC 2 cut(s) 275, 1136
BstUI CGCG 1 cut(s) 483
BstV1I GCAGC 4 cut(s) 60, 88, 407, 2118
BstV2I GAAGAC 4 cut(s) 437, 1256, 1515, 2804
BstX2I RGATCY 2 cut(s) 823, 2191
BstYI RGATCY 2 cut(s) 823, 2191
BsuRI GGCC 5 cut(s) 176, 200, 320, 2041, 2270
BtrI CACGTC 1 cut(s) 256
BtsIMutI CAGTG 4 cut(s) 1032, 1847, 2528, 2804
BtuMI TCGCGA 1 cut(s) 483
BveI ACCTGC 1 cut(s) 2524
Cac8I GCNNGC 3 cut(s) 178, 202, 2898
CciI TCATGA 1 cut(s) 241
CfoI GCGC 1 cut(s) 1272
Cfr13I GGNCC 6 cut(s) 198, 649, 701, 2039, 2268, 2877
Csp6I GTAC 7 cut(s) 375, 920, 1489, 1791, 1881, 1990, 2851
CviQI GTAC 7 cut(s) 375, 920, 1489, 1791, 1881, 1990, 2851
DdeI CTNAG 6 cut(s) 378, 820, 1743, 2048, 2712, 2746
DpnI GATC 6 cut(s) 62, 825, 1434, 1941, 2046, 2193
DpnII GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
DraI TTTAAA 2 cut(s) 1869, 2385
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 5 cut(s) 61, 828, 1122, 2471, 2804
EarI CTCTTC 5 cut(s) 61, 828, 1122, 2471, 2804
Eco130I CCWWGG 3 cut(s) 321, 1644, 2419
Eco147I AGGCCT 1 cut(s) 320
Eco24I GRGCYC 1 cut(s) 2224
Eco32I GATATC 1 cut(s) 2928
Eco47I GGWCC 3 cut(s) 649, 701, 2877
Eco57I CTGAAG 2 cut(s) 131, 2828
Eco72I CACGTG 1 cut(s) 2155
Eco88I CYCGRG 1 cut(s) 1137
Eco91I GGTNACC 2 cut(s) 46, 1051
EcoNI CCTNNNNNAGG 2 cut(s) 720, 2520
EcoO109I RGGNCCY 3 cut(s) 649, 701, 2039
EcoO65I GGTNACC 2 cut(s) 46, 1051
EcoRII CCWGG 2 cut(s) 27, 2082
EcoRV GATATC 1 cut(s) 2928
EcoT14I CCWWGG 3 cut(s) 321, 1644, 2419
EcoT22I ATGCAT 1 cut(s) 1623
EcoT38I GRGCYC 1 cut(s) 2224
ErhI CCWWGG 3 cut(s) 321, 1644, 2419
Esp3I CGTCTC 1 cut(s) 1569
FalI AAGNNNNNCTT 2 cut(s) 2208, 2240
FaqI GGGAC 3 cut(s) 100, 1369, 2539
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 5 cut(s) 74, 77, 421, 2009, 2132
FriOI GRGCYC 1 cut(s) 2224
Fsp4HI GCNGC 5 cut(s) 74, 77, 421, 2009, 2132
FspBI CTAG 6 cut(s) 671, 761, 965, 990, 1755, 2141
GlaI GCGC 1 cut(s) 1271
GluI GCNGC 5 cut(s) 74, 77, 421, 2009, 2132
GsaI CCCAGC 1 cut(s) 1704
GsuI CTGGAG 1 cut(s) 50
HaeIII GGCC 5 cut(s) 176, 200, 320, 2041, 2270
HapII CCGG 2 cut(s) 1340, 2783
HhaI GCGC 1 cut(s) 1272
Hin6I GCGC 1 cut(s) 1270
HinP1I GCGC 1 cut(s) 1270
HincII GTYRAC 4 cut(s) 55, 522, 758, 784
HindII GTYRAC 4 cut(s) 55, 522, 758, 784
HindIII AAGCTT 1 cut(s) 2210
HpaI GTTAAC 2 cut(s) 55, 522
HpaII CCGG 2 cut(s) 1340, 2783
HphI GGTGA 6 cut(s) 40, 94, 1681, 1925, 1994, 2900
Hpy99I CGWCG 4 cut(s) 257, 468, 483, 2626
HpyCH4III ACNGT 7 cut(s) 52, 1712, 2260, 2283, 2333, 2532, 2762
HpyCH4IV ACGT 7 cut(s) 255, 547, 1399, 1855, 2079, 2154, 2866
HpyF10VI GCNNNNNNNGC 4 cut(s) 417, 2038, 2276, 2366
HpyF3I CTNAG 6 cut(s) 378, 820, 1743, 2048, 2712, 2746
HpySE526I ACGT 7 cut(s) 255, 547, 1399, 1855, 2079, 2154, 2866
HspAI GCGC 1 cut(s) 1270
Kpn2I TCCGGA 1 cut(s) 1339
KspAI GTTAAC 2 cut(s) 55, 522
Kzo9I GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
LmnI GCTCC 6 cut(s) 31, 373, 1088, 1141, 1693, 2366
Lsp1109I GCAGC 4 cut(s) 60, 88, 407, 2118
LweI GCATC 6 cut(s) 142, 1748, 1767, 2080, 2252, 2424
MaeI CTAG 6 cut(s) 671, 761, 965, 990, 1755, 2141
MaeII ACGT 7 cut(s) 255, 547, 1399, 1855, 2079, 2154, 2866
MalI GATC 6 cut(s) 62, 825, 1434, 1941, 2046, 2193
MboI GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
MflI RGATCY 2 cut(s) 823, 2191
MhlI GDGCHC 4 cut(s) 275, 1136, 2224, 2281
MlyI GAGTC 5 cut(s) 123, 435, 514, 2111, 2437
MmeI TCCRAC 4 cut(s) 240, 1671, 2166, 2744
Mph1103I ATGCAT 1 cut(s) 1623
MroI TCCGGA 1 cut(s) 1339
MslI CAYNNNNRTG 2 cut(s) 140, 1730
MspA1I CMGCKG 2 cut(s) 79, 2325
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 2 cut(s) 1340, 2783
MspR9I CCNGG 2 cut(s) 29, 2084
Mva1269I GAATGC 2 cut(s) 2383, 2842
MvaI CCWGG 2 cut(s) 29, 2084
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 4 cut(s) 417, 2038, 2276, 2366
NdeII GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
NlaIV GGNNCC 5 cut(s) 199, 702, 980, 1030, 2269
NmeAIII GCCGAG 1 cut(s) 449
NmuCI GTSAC 6 cut(s) 46, 166, 190, 1473, 1718, 2327
NruI TCGCGA 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 1623
OliI CACNNNNGTG 1 cut(s) 140
PagI TCATGA 1 cut(s) 241
PceI AGGCCT 1 cut(s) 320
PctI GAATGC 2 cut(s) 2383, 2842
PfeI GAWTC 5 cut(s) 328, 1105, 2405, 2543, 2708
PflFI GACNNNGTC 3 cut(s) 779, 1401, 2530
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 5 cut(s) 75, 78, 422, 2010, 2133
PleI GAGTC 5 cut(s) 123, 434, 513, 2110, 2437
PmaCI CACGTG 1 cut(s) 2155
PmlI CACGTG 1 cut(s) 2155
PpsI GAGTC 5 cut(s) 123, 434, 513, 2110, 2437
Ppu21I YACGTR 1 cut(s) 2155
PpuMI RGGWCCY 2 cut(s) 649, 701
PshBI ATTAAT 4 cut(s) 18, 935, 1007, 2907
PsiI TTATAA 2 cut(s) 552, 1841
Psp1406I AACGTT 2 cut(s) 547, 2866
Psp5II RGGWCCY 2 cut(s) 649, 701
Psp6I CCWGG 2 cut(s) 27, 2082
PspCI CACGTG 1 cut(s) 2155
PspEI GGTNACC 2 cut(s) 46, 1051
PspFI CCCAGC 1 cut(s) 1700
PspGI CCWGG 2 cut(s) 27, 2082
PspN4I GGNNCC 5 cut(s) 199, 702, 980, 1030, 2269
PspPI GGNCC 6 cut(s) 198, 649, 701, 2039, 2268, 2877
PspPPI RGGWCCY 2 cut(s) 649, 701
PstI CTGCAG 1 cut(s) 78
PsuI RGATCY 2 cut(s) 823, 2191
PsyI GACNNNGTC 3 cut(s) 779, 1401, 2530
PvuII CAGCTG 1 cut(s) 79
RruI TCGCGA 1 cut(s) 483
RsaI GTAC 7 cut(s) 376, 921, 1490, 1792, 1882, 1991, 2852
RsaNI GTAC 7 cut(s) 375, 920, 1489, 1791, 1881, 1990, 2851
RseI CAYNNNNRTG 2 cut(s) 140, 1730
SatI GCNGC 5 cut(s) 74, 77, 421, 2009, 2132
Sau3AI GATC 6 cut(s) 60, 823, 1432, 1939, 2044, 2191
Sau96I GGNCC 6 cut(s) 198, 649, 701, 2039, 2268, 2877
ScaI AGTACT 1 cut(s) 1882
SchI GAGTC 5 cut(s) 123, 435, 514, 2111, 2437
ScrFI CCNGG 2 cut(s) 29, 2084
SduI GDGCHC 4 cut(s) 275, 1136, 2224, 2281
SfaNI GCATC 6 cut(s) 142, 1748, 1767, 2080, 2252, 2424
SfcI CTRYAG 2 cut(s) 74, 2332
SinI GGWCC 3 cut(s) 649, 701, 2877
SmiMI CAYNNNNRTG 2 cut(s) 140, 1730
SmlI CTYRAG 3 cut(s) 284, 1022, 1077
SmoI CTYRAG 3 cut(s) 284, 1022, 1077
SpeI ACTAGT 1 cut(s) 760
SseBI AGGCCT 1 cut(s) 320
SsiI CCGC 5 cut(s) 118, 155, 491, 2008, 2323
SspI AATATT 1 cut(s) 1047
SspMI CTAG 6 cut(s) 671, 761, 965, 990, 1755, 2141
StuI AGGCCT 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 27, 2082
StyI CCWWGG 3 cut(s) 321, 1644, 2419
TaaI ACNGT 7 cut(s) 52, 1712, 2260, 2283, 2333, 2532, 2762
TaiI ACGT 7 cut(s) 258, 550, 1402, 1858, 2082, 2157, 2869
TaqI TCGA 4 cut(s) 466, 634, 2441, 2608
TaqII GACCGA 1 cut(s) 34
TatI WGTACW 4 cut(s) 1488, 1880, 1989, 2850
TauI GCSGC 1 cut(s) 2011
TfiI GAWTC 5 cut(s) 328, 1105, 2405, 2543, 2708
TscAI CASTG 4 cut(s) 1039, 1854, 2535, 2811
TseFI GTSAC 6 cut(s) 46, 166, 190, 1473, 1718, 2327
TseI GCWGC 4 cut(s) 73, 76, 420, 2131
Tsp45I GTSAC 6 cut(s) 46, 166, 190, 1473, 1718, 2327
TspGWI ACGGA 5 cut(s) 185, 329, 452, 2362, 2871
TspRI CASTG 4 cut(s) 1039, 1854, 2535, 2811
Tth111I GACNNNGTC 3 cut(s) 779, 1401, 2530
Vha464I CTTAAG 1 cut(s) 284
VneI GTGCAC 2 cut(s) 271, 1132
VpaK11BI GGWCC 3 cut(s) 649, 701, 2877
VspI ATTAAT 4 cut(s) 18, 935, 1007, 2907
XagI CCTNNNNNAGG 2 cut(s) 720, 2520
XapI RAATTY 7 cut(s) 279, 950, 985, 1335, 1783, 1870, 2960
XcmI CCANNNNNNNNNTGG 1 cut(s) 2236
XspI CTAG 6 cut(s) 671, 761, 965, 990, 1755, 2141
ZrmI AGTACT 1 cut(s) 1882
Zsp2I ATGCAT 1 cut(s) 1623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.