Rorug02G0371100

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006255
Physical Location & Seq
Reverse (-)
47350041 .. 47360433
10393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0371100.1

Sequence Viewer

Length: 4959 bp
ATGGCCCCAAAATCAGGCAGAGGCAAAAACAACAAGGCCAAGTCTGACAAGAAGAAGAAGGAAGATAAGGTTCCTAGTGTTCTTGACATCACTGTCATTACTCCATATGACACACAAGTTATTCTTAAGGGTATCTCCACTGACAAGATACTAGATGTTAAAAGACTACTAGCTGTGAATGTAGAGACATGTCATCTTACAAACTATTCCTTGTCTCATGAAGTGAAAGGGAAGAGACTGAATGAAAGGGTGGAGGTGGTTAGTCTGAAACCCTGTCTTCTGAAAATGGTTGAAGAGGACTATAGCGATGAAGCCGAAGCTGTTGCCCACGTGCGGCGTCTGCTGGACCTGGTGGCCTGCACGACCCGATTCGCCAAGCCCAAACGGTCCGCTGCCGCCCCGGACTCCAAGTCCAAGAAGAGCGGCACCAAACCCGTCAACAAGAACTCCGGCCCATCCTCACCGTCCGTTTCGGCTATCTCAGAGAGCCTCGGGATGGTCGCCATTCACCCGACCCCGAAGCTCTCCGACTTCTACGACTTCTTCTCTTTCTCACACCTCGCCCCTCCCATTCTCCATTTACGGAGGTGTGACGTGGACGACGCACAGGAGAGGCGCGACGGTGACTATTTTCACATGCAGATTAAGATTTGCAATGGGAAGCAAGTACAAGTGGTTGCATCAGTAAAAGGGTTCTACACAGTGGGAAAGCAGTTTTTACAAAGCCACTCATTGGTGGATCTTCTGCAACAGCTCAGTAGAGCTTTTGCTAATGCATATGAATCCTTGATGAAAGCTTTTGTAGAGCATAACAAGTTTGGTGACCTTCCATATGGTTTTCGAGCAAACACATGGCTTGTTCCTCCATCTGTTGCCGAGTCTCCATCAAATTTTCCATCCCTGCCGACTGAAGATGAAAATTGGGGAGGAAATGGTGGAGGCCAGGGAAGAAATGGTGAATATGATCGTCGACCATGGGCTACTGACTTTGCAATTTTGGCTTGCCTGCCTTGCAAAACTGAGGAGGAGAGGGTTGTTCGAGACCGAAAAGCATTTTTGCTTCATAGTAAATTCATTGATGTATCGGTCTTTAAAGCTGCTGCTGCAATACGTGGTATAATAGATTCTACTGTGAATGGAAAGGAGACAACCAATTGTTCTTCGGGCTCTGTTCTTTATGAGGAGCGTGTTGGAGACTTGTCCATTGTTGTAAAACGTGATATAACAGATCCATTGTCAAAGTCCGAAGTAATAGTTAGTGGCGATCATCCATGCAGTACCCGGGAAGTTGCTCAGAGGTGTTTACTCAAGGGTTTAACTTCTGATGAGAGTGTGGTTGTTCATGATACACCCTCCTTGGGTGTGGTCAATGTAAGGCACTGTGGATACATTGCAACAGTTAAAGTTGTTGGTAAAATAAAGAAGGGAAGCTGTGAGGCTAAAGATATTGACATTGAAGATCAACCAGATGGAGGAGCCAATTCTCTTAATGTGAACAGCCTGAGGGTTCTGCTTCAAAAGTTCAACACTGAATCAGTGGATCACTCTAATTCAGATGGTTTAGAAACTTCTAGGTGTCTGGTTCAAAGAGTATTAAAAGAGAGCTTGACGAAGTTAGAGGATGAGCCTGCCAATTCTGAGAGGTCTATCAGATGGGAACTTGGTTCTTGCTGGTTGCAACATCTACAAAAGCAGGAAACACCAGTAAACAATTCTGATAGTCCCGAGGATGATATTGAGGCGGTTGAACCAGTTGTTAAAGGTCTAGGGAAGCAATTTAAATTTTTGAAGAAGAGGGAAAAGAAAACAAGTACTGAAGCTGGAACATATGATGAGGAAGACATTGATGCTAATTCTTTGAGTCTAAATGGGGGATCAGATAAATTAGAGGTAAACAATGGCGAGAGTCATGAGATCAGCAATGAGGCAGAATTGAAGACTTTAATTTCAGAAGAAGCCTATTTGCGCCTGAAAGAAACTGGAACTGGTCTTCATCTAAAGTCAGCAGATGAGCTTATCAAGATGGCACACAAATATTATGATGAAGTTGCCTTGCCAAAGCTGGTAACAGACTTTGGATCACTTGAACTCTCTCCAGTTGATGGCCGTACGCTGACAGATTTCATGCATTTAAGGGGGCTGAAAATGCGCTCTTTGGGTCGAGTGGTTGAACTGTCGGAGAAACTTCCTCATATACAGTCTCTTTGTATTCATGAGATGATTACTCGAGCTTTCAAGCATGTGCTTGAAGCAGTCATTGCTTCTGTTGGCAAAATAACTGACTTGTCTGCGGCAATAGCTGCAACCCTAAATTTTCTACTTGGAGGCTCTGCGATGGATGATGATGTACTCAAATTGCAATGGCTACGAATATTCCTGGCCCGAAAATTTGGTTGGGCACTGAAAGATGAATTTCAGCACTTGAGGAAGTTATCAATTCTCCGAGGACTTTGCCATAAGGTTGGGCTGGAGTTGGCCCCAAGAGATTATGACATGGAATGCCATAATCCTTTCAGCATATACGACATTATTAGCATGGTTCCAGTGTGTAAGCATGTAGCATGTTCTTCAGCTGATGGACGAAATTTGTTGGAGTCATCTAAAATTGCTCTTGATAAAGGAAAGTTAGAGGATGCTGTACATTTCGGAACAAAGGCACTAGCAAAGATGATAGCTGTCTGTGGCCCCTATCATCGAGTTACTGCAAGTGCTTATAGCCTTTTAGCAGTAGTCCTTTACCACACGGGTGACTTTAATCAGGCAACCATTTATCAGCAGAAGGCTTTAGCTATTAATGAAAGGGAACTTGGGCTGGACCATCCAGATACCATGAAAAGCTATGGGGATCTTTCCGTTTTTTACTACCGTCTTCAATACATCGAATTGGCTTTGAAGTATGTGAATCGTGCGTTGTTTCTTCTTCATTTTACATGTGGACTATCTCACCCTAACACAGCTGCGACATACATAAATGTGGCTATGATGGAGGAGGGCATGGGAAATGTCCATGTTGCTCTCAGATACCTACATGAAGCTTTGAAGTGTAACCAGAGACTATTAGGAGCAGATCACATACAGACTGCTGCAAGCTATCATGCCATAGCCATAGCTCTTTCATTGATGGAAGCATATTCTCTAAGTGTGCAACATGAGCAAACAACACTTAGGATACTTCAAGATAAACTTGGACCTGAGGATCTTCGTACTCAGGATGCAGCAGCATGGCTTGAATATTTTGAATCTAAATCTCTAGAGCAGCAAGAAGCAGCACGAAATGGATCTCCAAAACCTGATGCCTTGATTGCAAGCAAAGGTCACCTTAGTGTGTCAGATCTCCTTGATTACATTAGCCCTGATCAAGATTCAAAAGTAAATGATGCACATAGGAAGCAGCGACGTGCAAAGGTTTATCAGTCGAGTGATACCATCTATGAGGCACATCAAGATGTAATAGCTGACGATGACTTGCCAAGTGATGGCCTTGAAAACCCTATGATTTTGATAGATGATAATACTGAAGTAGTTGAGGAAAGATGGGTTCATCAGGGACTGGAGGAAAAAGATGATGTCTCTAGAAATGGTTTGTCAGTTGCAAGTGTAACTGCCGAAGAAACAACTTCAGATGAAGGATGGCAAGAAGCCAATTCAAAGGGGAGGTCTGGGAATACCACTACTGGGCGGAGGTTCAGTAGAAGGAGGCCAGACAGTGAATCTTCACACTTTAGAGAAAGCAAATACTCGAGGGAAGTCAAATCCTCATCACAGACTGCAGCTGTGAAGAGCTTTCTAAATGATTCATCTTCATCAAAGCAATCAAAGGTTCGTACTGTGAGTACTGGAGAGGATTCAGTTAGACTTCAGTCCAAAACTACTGTTTCTAAAGTCTTCTCTACTCCAGCCACTCTTACGAACTTAACTTCAAAATCTGTATCTTACAAACAAGTAGCTTTGGCACCTCCAGGTACTGTTCTAAAGTCATTGCTGGATAAAGTTGACGACCTGAATGTGGAAGAATCTGAAACAAAGGAGTGTAACCCTCCACCTGAGACATTGAAAACTGAAGAAAGCATAGGTGTAGTTGAACTCATTCCAAATGATGAGAACGAAGGAACACATGCAAGTTCAACCCAGTTGGAAGAAACAGGCCCTGAAACTGTGGAAGAAAGGTCTGCAGAGAAAAATGGAAGTAAGCTGTCAGCTGCAGCAGAACCATTCAATCCAAGACCACTCACCATGACTCGCCCTCTAAATCCAGTTGCTGTTACAAGTGTATATGACGTGAGAGCCAGCCAAGCAATGCTTTCTGCGCCTGCGCTCCCCCCAGTAGCTGCTAGAGTTCCTTGTGGTCCTCGGTCACCTTTGTATTATAAAACCAATTATTCTTTTCGCTTGAGGCAAGGTGTTCAAAAATTTCGAAGACCCCTTTCAGAAAGAAGTGGATCAGGACCTCCAAGAATCATGAATCCACATGCTCCAGAGTTTGTTCCTGGGAGAACTTTGCAAGCAGATTACATGAATGAATATGCAGAATTTCCAACTGAAGCAAATTCTTCATTTGAAATGAATAGGGCTGAAGAGACGGATGAAAAATCGAATGGTAAAGCTGAGAGAAAGAGCATCTCTGAGTCTGAGAAGTCAGAGCTTGCAAGGCAGATATTGCTGAGCTTCATAGTAAAATCTGTCCAGCACAACAAGGATTCTGAAAGTGAAAGTAAGCAGGAAAATCATTTAGATGCCGTAGAGAAGGACAGTGCAATTATAAAGATTCATTATGGAAATGAAGGAAAGGACTTAGTTTCTCAATCCAGTGACTGTGATCAATCAAAAACAACGGATGTAGATACAAATGAAGTTGGTGATAGTGAAGGATTTACAGTTGTGACAAAGAGGAGAAGAAGCCGGCAGCAGTTCAGAAGTGAGTTGTACAATCAGCAATCCATCTCGGCTTCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1652

Amino Acids

183.11

Weight (kDa)

6.03

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 47 - 118 1.4e-08 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 697 - 779 1.5e-18 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 870 - 932 2.5e-08 Tetratricopeptide repeat
TPR_NPHP3 PF24885 872 - 971 2.4e-06 Nephrocystin-3 TPR domain
TPR_12 PF13424 901 - 971 6.8e-11 Tetratricopeptide repeat
TPR_10 PF13374 901 - 942 2.5e-10 Tetratricopeptide repeat
TPR_12 PF13424 985 - 1059 3.3e-11 Tetratricopeptide repeat
TPR_10 PF13374 985 - 1025 2.9e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4368, 4760
AasI GACNNNNNNGTC 1 cut(s) 92
AccB1I GGYRCC 2 cut(s) 425, 3952
AccB7I CCANNNNNTGG 3 cut(s) 733, 2102, 3476
AccBSI CCGCTC 1 cut(s) 423
AccI GTMKAC 1 cut(s) 970
AccII CGCG 1 cut(s) 618
AciI CCGC 7 cut(s) 334, 390, 396, 423, 1742, 2291, 3679
AclWI GGATC 9 cut(s) 747, 1223, 1548, 1882, 2086, 2853, 3204, 3286, 4447
AcoI YGGCCR 1 cut(s) 2104
AcuI CTGAAG 9 cut(s) 930, 1836, 2553, 3537, 3603, 3842, 4080, 4560, 4593
AcvI CACGTG 1 cut(s) 331
AcyI GRCGYC 1 cut(s) 337
AflII CTTAAG 1 cut(s) 125
AflIII ACRYGT 2 cut(s) 188, 2930
AhdI GACNNNNNGTC 1 cut(s) 409
AjiI CACGTC 3 cut(s) 595, 3398, 4279
AjnI CCWGG 5 cut(s) 348, 942, 2376, 3958, 4486
AjuI GAANNNNNNNTTGG 4 cut(s) 2377, 2409, 2790, 2822
AleI CACNNNNGTG 2 cut(s) 2745, 3321
AlwI GGATC 9 cut(s) 747, 1223, 1548, 1882, 2086, 2853, 3204, 3286, 4447
AlwNI CAGNNNCTG 6 cut(s) 3550, 3965, 4148, 4259, 4328, 4812
Ama87I CYCGRG 5 cut(s) 491, 1281, 1724, 2226, 3739
ArsI GACNNNNNNTTYG 2 cut(s) 3641, 3673
AseI ATTAAT 1 cut(s) 2793
Asp700I GAANNNNTTC 1 cut(s) 4086
AspLEI GCGC 5 cut(s) 618, 1968, 2151, 4309, 4315
AsuC2I CCSGG 3 cut(s) 401, 1282, 1283
AsuII TTCGAA 1 cut(s) 4414
AvaI CYCGRG 5 cut(s) 491, 1281, 1724, 2226, 3739
AvaII GGWCC 6 cut(s) 346, 387, 2815, 3188, 4346, 4445
AxyI CCTNAGG 2 cut(s) 1502, 3192
BaeGI GKGCMC 1 cut(s) 2401
BanI GGYRCC 2 cut(s) 425, 3952
BanII GRGCYC 1 cut(s) 1169
BbrPI CACGTG 1 cut(s) 331
BbsI GAAGAC 7 cut(s) 269, 1845, 1943, 1982, 2861, 3877, 4423
BceAI ACGGC 2 cut(s) 2091, 4721
BcgI CGANNNNNNTGC 4 cut(s) 305, 339, 2433, 2467
BciT130I CCWGG 5 cut(s) 350, 944, 2378, 3960, 4488
BciVI GTATCC 2 cut(s) 1379, 3162
BclI TGATCA 2 cut(s) 3355, 4816
BcnI CCSGG 3 cut(s) 401, 1282, 1283
BfaI CTAG 9 cut(s) 75, 152, 170, 1572, 1766, 2660, 3251, 3573, 4332
BfmI CTRYAG 4 cut(s) 301, 3768, 4170, 4200
BfrI CTTAAG 1 cut(s) 125
BfuI GTATCC 2 cut(s) 1379, 3162
BglII AGATCT 1 cut(s) 3331
BlpI GCTNAGC 1 cut(s) 4661
BmcAI AGTACT 2 cut(s) 1813, 3835
Bme1390I CCNGG 8 cut(s) 350, 401, 944, 1282, 1283, 2378, 3960, 4488
Bme18I GGWCC 6 cut(s) 346, 387, 2815, 3188, 4346, 4445
BmeRI GACNNNNNGTC 1 cut(s) 409
BmeT110I CYCGRG 5 cut(s) 491, 1281, 1724, 2226, 3739
BmgBI CACGTC 3 cut(s) 595, 3398, 4279
BmiI GGNNCC 8 cut(s) 6, 72, 427, 1477, 2478, 2541, 2686, 3954
BmrFI CCNGG 8 cut(s) 350, 401, 944, 1282, 1283, 2378, 3960, 4488
BmrI ACTGGG 3 cut(s) 3684, 4123, 4316
BmsI GCATC 8 cut(s) 689, 1837, 2623, 3202, 3283, 3367, 4626, 4723
BmuI ACTGGG 3 cut(s) 3684, 4123, 4316
BoxI GACNNNNGTC 1 cut(s) 3859
BpiI GAAGAC 7 cut(s) 269, 1845, 1943, 1982, 2861, 3877, 4423
BpmI CTGGAG 7 cut(s) 2079, 2489, 3572, 3858, 3879, 3942, 4458
Bpu1102I GCTNAGC 1 cut(s) 4661
Bpu14I TTCGAA 1 cut(s) 4414
BpuEI CTTGAG 3 cut(s) 1292, 2443, 4411
BpuMI CCSGG 3 cut(s) 401, 1282, 1283
BsaAI YACGTR 2 cut(s) 331, 1112
BsaHI GRCGYC 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 1035
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 3673, 3703
Bse118I RCCGGY 1 cut(s) 4899
Bse21I CCTNAGG 2 cut(s) 1502, 3192
Bse3DI GCAATG 7 cut(s) 661, 1389, 1927, 2256, 2366, 3977, 4302
BseBI CCWGG 5 cut(s) 350, 944, 2378, 3960, 4488
BseMI GCAATG 7 cut(s) 661, 1389, 1927, 2256, 2366, 3977, 4302
BseRI GAGGAG 6 cut(s) 1037, 1040, 1196, 1488, 3002, 4903
BseSI GKGCMC 1 cut(s) 2401
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 618
BshNI GGYRCC 2 cut(s) 425, 3952
BsiHKCI CYCGRG 5 cut(s) 491, 1281, 1724, 2226, 3739
BsiSI CCGG 4 cut(s) 401, 450, 1282, 4900
BsiWI CGTACG 1 cut(s) 2108
BslFI GGGAC 2 cut(s) 1707, 3561
BsmBI CGTCTC 1 cut(s) 4571
BsmFI GGGAC 2 cut(s) 1707, 3561
BsmI GAATGC 1 cut(s) 2504
Bso31I GGTCTC 1 cut(s) 1035
BsoBI CYCGRG 5 cut(s) 491, 1281, 1724, 2226, 3739
Bsp119I TTCGAA 1 cut(s) 4414
Bsp1286I GDGCHC 2 cut(s) 1169, 2401
Bsp1407I TGTACA 2 cut(s) 2638, 4923
Bsp1720I GCTNAGC 1 cut(s) 4661
Bsp19I CCATGG 1 cut(s) 974
BspACI CCGC 7 cut(s) 334, 390, 396, 423, 1742, 2291, 3679
BspFNI CGCG 1 cut(s) 618
BspHI TCATGA 5 cut(s) 217, 1342, 1909, 2212, 4458
BspLI GGNNCC 8 cut(s) 6, 72, 427, 1477, 2478, 2541, 2686, 3954
BspMAI CTGCAG 3 cut(s) 3772, 4174, 4204
BspPI GGATC 9 cut(s) 747, 1223, 1548, 1882, 2086, 2853, 3204, 3286, 4447
BspQI GCTCTTC 2 cut(s) 413, 3773
BspT104I TTCGAA 1 cut(s) 4414
BspT107I GGYRCC 2 cut(s) 425, 3952
BspTI CTTAAG 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 1035
BsrBI CCGCTC 1 cut(s) 423
BsrDI GCAATG 7 cut(s) 661, 1389, 1927, 2256, 2366, 3977, 4302
BsrFI RCCGGY 1 cut(s) 4899
BsrGI TGTACA 2 cut(s) 2638, 4923
BssAI RCCGGY 1 cut(s) 4899
BssNI GRCGYC 1 cut(s) 337
BssT1I CCWWGG 2 cut(s) 974, 1356
Bst2UI CCWGG 5 cut(s) 350, 944, 2378, 3960, 4488
Bst6I CTCTTC 6 cut(s) 227, 288, 413, 1787, 3773, 4569
BstACI GRCGYC 1 cut(s) 337
BstAFI CTTAAG 1 cut(s) 125
BstAPI GCANNNNNTGC 3 cut(s) 2258, 2300, 4657
BstAUI TGTACA 2 cut(s) 2638, 4923
BstBAI YACGTR 2 cut(s) 331, 1112
BstBI TTCGAA 1 cut(s) 4414
BstDSI CCRYGG 1 cut(s) 974
BstEII GGTNACC 3 cut(s) 821, 3314, 4353
BstFNI CGCG 1 cut(s) 618
BstHHI GCGC 5 cut(s) 618, 1968, 2151, 4309, 4315
BstNI CCWGG 5 cut(s) 350, 944, 2378, 3960, 4488
BstNSI RCATGY 8 cut(s) 192, 640, 2243, 2558, 2565, 2934, 4118, 4472
BstPAI GACNNNNGTC 1 cut(s) 3859
BstPI GGTNACC 3 cut(s) 821, 3314, 4353
BstSCI CCNGG 8 cut(s) 348, 399, 942, 1280, 1281, 2376, 3958, 4486
BstSFI CTRYAG 4 cut(s) 301, 3768, 4170, 4200
BstSLI GKGCMC 1 cut(s) 2401
BstUI CGCG 1 cut(s) 618
BstV2I GAAGAC 7 cut(s) 269, 1845, 1943, 1982, 2861, 3877, 4423
BstX2I RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
BstXI CCANNNNNNTGG 1 cut(s) 2462
BstYI RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
Bsu36I CCTNAGG 2 cut(s) 1502, 3192
BsuI GTATCC 2 cut(s) 1379, 3162
BtgI CCRYGG 1 cut(s) 974
BtgZI GCGATG 2 cut(s) 321, 2348
BtrI CACGTC 3 cut(s) 595, 3398, 4279
CaiI CAGNNNCTG 6 cut(s) 3550, 3965, 4148, 4259, 4328, 4812
CciI TCATGA 5 cut(s) 217, 1342, 1909, 2212, 4458
CfoI GCGC 5 cut(s) 618, 1968, 2151, 4309, 4315
Cfr10I RCCGGY 1 cut(s) 4899
Cfr9I CCCGGG 1 cut(s) 1281
CpoI CGGWCCG 1 cut(s) 387
CseI GACGC 2 cut(s) 326, 611
CsiI ACCWGGT 1 cut(s) 348
CspI CGGWCCG 1 cut(s) 387
DraI TTTAAA 2 cut(s) 1093, 1780
DrdI GACNNNNNNGTC 1 cut(s) 92
DriI GACNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 2104
Eam1104I CTCTTC 6 cut(s) 227, 288, 413, 1787, 3773, 4569
Eam1105I GACNNNNNGTC 1 cut(s) 409
EarI CTCTTC 6 cut(s) 227, 288, 413, 1787, 3773, 4569
EciI GGCGGA 1 cut(s) 3694
Eco130I CCWWGG 2 cut(s) 974, 1356
Eco24I GRGCYC 1 cut(s) 1169
Eco31I GGTCTC 1 cut(s) 1035
Eco47I GGWCC 6 cut(s) 346, 387, 2815, 3188, 4346, 4445
Eco57I CTGAAG 9 cut(s) 930, 1836, 2553, 3537, 3603, 3842, 4080, 4560, 4593
Eco72I CACGTG 1 cut(s) 331
Eco81I CCTNAGG 2 cut(s) 1502, 3192
Eco88I CYCGRG 5 cut(s) 491, 1281, 1724, 2226, 3739
Eco91I GGTNACC 3 cut(s) 821, 3314, 4353
EcoO109I RGGNCCY 2 cut(s) 4145, 4445
EcoO65I GGTNACC 3 cut(s) 821, 3314, 4353
EcoRII CCWGG 5 cut(s) 348, 942, 2376, 3958, 4486
EcoT14I CCWWGG 2 cut(s) 974, 1356
EcoT22I ATGCAT 2 cut(s) 778, 2130
EcoT38I GRGCYC 1 cut(s) 1169
ErhI CCWWGG 2 cut(s) 974, 1356
Esp3I CGTCTC 1 cut(s) 4571
FalI AAGNNNNNCTT 8 cut(s) 108, 140, 3168, 3200, 3303, 3335, 4284, 4316
FaqI GGGAC 2 cut(s) 1707, 3561
FauNDI CATATG 4 cut(s) 106, 778, 832, 1828
FbaI TGATCA 2 cut(s) 3355, 4816
FblI GTMKAC 1 cut(s) 970
FriOI GRGCYC 1 cut(s) 1169
FspBI CTAG 9 cut(s) 75, 152, 170, 1572, 1766, 2660, 3251, 3573, 4332
GlaI GCGC 5 cut(s) 617, 1967, 2150, 4308, 4314
GsuI CTGGAG 7 cut(s) 2079, 2489, 3572, 3858, 3879, 3942, 4458
HapII CCGG 4 cut(s) 401, 450, 1282, 4900
HgaI GACGC 2 cut(s) 326, 611
HhaI GCGC 5 cut(s) 618, 1968, 2151, 4309, 4315
Hin1I GRCGYC 1 cut(s) 337
Hin6I GCGC 5 cut(s) 616, 1966, 2149, 4307, 4313
HinP1I GCGC 5 cut(s) 616, 1966, 2149, 4307, 4313
HincII GTYRAC 3 cut(s) 439, 971, 3994
HindII GTYRAC 3 cut(s) 439, 971, 3994
HindIII AAGCTT 2 cut(s) 795, 3033
HpaII CCGG 4 cut(s) 401, 450, 1282, 4900
Hpy166II GTNNAC 9 cut(s) 439, 598, 971, 1304, 1495, 1708, 1894, 2936, 3994
Hpy8I GTNNAC 9 cut(s) 439, 598, 971, 1304, 1495, 1708, 1894, 2936, 3994
Hpy99I CGWCG 4 cut(s) 605, 623, 972, 3399
HpyCH4IV ACGT 6 cut(s) 330, 594, 1111, 1216, 3397, 4278
HpySE526I ACGT 6 cut(s) 330, 594, 1111, 1216, 3397, 4278
Hsp92I GRCGYC 1 cut(s) 337
HspAI GCGC 5 cut(s) 616, 1966, 2149, 4307, 4313
KroI GCCGGC 1 cut(s) 4899
KroNI GCCGGC 1 cut(s) 4901
Ksp22I TGATCA 2 cut(s) 3355, 4816
LguI GCTCTTC 2 cut(s) 413, 3773
LmnI GCTCC 5 cut(s) 1183, 1475, 3062, 4320, 4477
LweI GCATC 8 cut(s) 689, 1837, 2623, 3202, 3283, 3367, 4626, 4723
MabI ACCWGGT 1 cut(s) 348
MaeI CTAG 9 cut(s) 75, 152, 170, 1572, 1766, 2660, 3251, 3573, 4332
MaeII ACGT 6 cut(s) 330, 594, 1111, 1216, 3397, 4278
MbiI CCGCTC 1 cut(s) 423
MfeI CAATTG 1 cut(s) 1153
MflI RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
MhlI GDGCHC 2 cut(s) 1169, 2401
MlyI GAGTC 7 cut(s) 398, 887, 1870, 1915, 2603, 4231, 4634
MmeI TCCRAC 6 cut(s) 552, 1171, 2157, 2571, 4113, 4559
Mph1103I ATGCAT 2 cut(s) 778, 2130
MroNI GCCGGC 1 cut(s) 4899
MroXI GAANNNNTTC 1 cut(s) 4086
MslI CAYNNNNRTG 5 cut(s) 2745, 2972, 3321, 3444, 4731
MspA1I CMGCKG 5 cut(s) 392, 2573, 2957, 3773, 4199
MspCI CTTAAG 1 cut(s) 125
MspI CCGG 4 cut(s) 401, 450, 1282, 4900
MspR9I CCNGG 8 cut(s) 350, 401, 944, 1282, 1283, 2378, 3960, 4488
MunI CAATTG 1 cut(s) 1153
Mva1269I GAATGC 1 cut(s) 2504
MvaI CCWGG 5 cut(s) 350, 944, 2378, 3960, 4488
MvnI CGCG 1 cut(s) 618
NaeI GCCGGC 1 cut(s) 4901
NciI CCSGG 3 cut(s) 401, 1282, 1283
NcoI CCATGG 1 cut(s) 974
NdeI CATATG 4 cut(s) 106, 778, 832, 1828
NgoMIV GCCGGC 1 cut(s) 4899
NlaIV GGNNCC 8 cut(s) 6, 72, 427, 1477, 2478, 2541, 2686, 3954
NmeAIII GCCGAG 2 cut(s) 901, 4922
NmuCI GTSAC 8 cut(s) 590, 623, 821, 2747, 3314, 4353, 4808, 4879
NsiI ATGCAT 2 cut(s) 778, 2130
NspI RCATGY 8 cut(s) 192, 640, 2243, 2558, 2565, 2934, 4118, 4472
NspV TTCGAA 1 cut(s) 4414
OliI CACNNNNGTG 2 cut(s) 2745, 3321
PaeR7I CTCGAG 2 cut(s) 2226, 3739
PagI TCATGA 5 cut(s) 217, 1342, 1909, 2212, 4458
PciI ACATGT 2 cut(s) 188, 2930
PciSI GCTCTTC 2 cut(s) 413, 3773
PctI GAATGC 1 cut(s) 2504
PdiI GCCGGC 1 cut(s) 4901
PdmI GAANNNNTTC 1 cut(s) 4086
Pfl23II CGTACG 1 cut(s) 2108
PflMI CCANNNNNTGG 3 cut(s) 733, 2102, 3476
PleI GAGTC 7 cut(s) 398, 886, 1869, 1914, 2602, 4231, 4633
PmaCI CACGTG 1 cut(s) 331
PmlI CACGTG 1 cut(s) 331
PpsI GAGTC 7 cut(s) 398, 886, 1869, 1914, 2602, 4231, 4633
Ppu21I YACGTR 2 cut(s) 331, 1112
PpuMI RGGWCCY 1 cut(s) 4445
PscI ACATGT 2 cut(s) 188, 2930
PshAI GACNNNNGTC 1 cut(s) 3859
PshBI ATTAAT 1 cut(s) 2793
PsiI TTATAA 2 cut(s) 4368, 4760
Psp5II RGGWCCY 1 cut(s) 4445
Psp6I CCWGG 5 cut(s) 348, 942, 2376, 3958, 4486
PspCI CACGTG 1 cut(s) 331
PspEI GGTNACC 3 cut(s) 821, 3314, 4353
PspGI CCWGG 5 cut(s) 348, 942, 2376, 3958, 4486
PspLI CGTACG 1 cut(s) 2108
PspN4I GGNNCC 8 cut(s) 6, 72, 427, 1477, 2478, 2541, 2686, 3954
PspPPI RGGWCCY 1 cut(s) 4445
PspXI VCTCGAGB 2 cut(s) 2226, 3739
PstI CTGCAG 3 cut(s) 3772, 4174, 4204
PstNI CAGNNNCTG 6 cut(s) 3550, 3965, 4148, 4259, 4328, 4812
PsuI RGATCY 6 cut(s) 739, 1228, 2845, 3196, 3278, 3331
PvuII CAGCTG 4 cut(s) 2573, 2957, 3773, 4199
RseI CAYNNNNRTG 5 cut(s) 2745, 2972, 3321, 3444, 4731
Rsr2I CGGWCCG 1 cut(s) 387
RsrII CGGWCCG 1 cut(s) 387
SalI GTCGAC 1 cut(s) 969
SapI GCTCTTC 2 cut(s) 413, 3773
ScaI AGTACT 2 cut(s) 1813, 3835
SchI GAGTC 7 cut(s) 398, 887, 1870, 1915, 2603, 4231, 4634
ScrFI CCNGG 8 cut(s) 350, 401, 944, 1282, 1283, 2378, 3960, 4488
SduI GDGCHC 2 cut(s) 1169, 2401
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 8 cut(s) 689, 1837, 2623, 3202, 3283, 3367, 4626, 4723
SfcI CTRYAG 4 cut(s) 301, 3768, 4170, 4200
Sfr274I CTCGAG 2 cut(s) 2226, 3739
SfuI TTCGAA 1 cut(s) 4414
SinI GGWCC 6 cut(s) 346, 387, 2815, 3188, 4346, 4445
SlaI CTCGAG 2 cut(s) 2226, 3739
SmaI CCCGGG 1 cut(s) 1283
SmiI ATTTAAAT 1 cut(s) 1780
SmiMI CAYNNNNRTG 5 cut(s) 2745, 2972, 3321, 3444, 4731
SmlI CTYRAG 6 cut(s) 125, 1307, 2226, 2422, 3739, 4390
SmoI CTYRAG 6 cut(s) 125, 1307, 2226, 2422, 3739, 4390
SsiI CCGC 7 cut(s) 334, 390, 396, 423, 1742, 2291, 3679
SspI AATATT 3 cut(s) 2036, 2373, 3233
SspMI CTAG 9 cut(s) 75, 152, 170, 1572, 1766, 2660, 3251, 3573, 4332
StyD4I CCNGG 8 cut(s) 348, 399, 942, 1280, 1281, 2376, 3958, 4486
StyI CCWWGG 2 cut(s) 974, 1356
SwaI ATTTAAAT 1 cut(s) 1780
TaiI ACGT 6 cut(s) 333, 597, 1114, 1219, 3400, 4281
TaqII GACCGA 3 cut(s) 1059, 1075, 4341
TatI WGTACW 6 cut(s) 667, 1811, 2347, 2638, 3833, 4923
TauI GCSGC 4 cut(s) 337, 398, 426, 2294
TseFI GTSAC 8 cut(s) 590, 623, 821, 2747, 3314, 4353, 4808, 4879
Tsp45I GTSAC 8 cut(s) 590, 623, 821, 2747, 3314, 4353, 4808, 4879
TspGWI ACGGA 5 cut(s) 457, 598, 2842, 4595, 4847
TspMI CCCGGG 1 cut(s) 1281
Van91I CCANNNNNTGG 3 cut(s) 733, 2102, 3476
Vha464I CTTAAG 1 cut(s) 125
VpaK11BI GGWCC 6 cut(s) 346, 387, 2815, 3188, 4346, 4445
VspI ATTAAT 1 cut(s) 2793
XbaI TCTAGA 2 cut(s) 3250, 3572
XceI RCATGY 8 cut(s) 192, 640, 2243, 2558, 2565, 2934, 4118, 4472
XcmI CCANNNNNNNNNTGG 1 cut(s) 950
XhoI CTCGAG 2 cut(s) 2226, 3739
XmaI CCCGGG 1 cut(s) 1281
XmiI GTMKAC 1 cut(s) 970
XmnI GAANNNNTTC 1 cut(s) 4086
XspI CTAG 9 cut(s) 75, 152, 170, 1572, 1766, 2660, 3251, 3573, 4332
ZrmI AGTACT 2 cut(s) 1813, 3835
Zsp2I ATGCAT 2 cut(s) 778, 2130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.