Rroxscaffold_2G00103610

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0022652
Physical Location & Seq
Reverse (-)
26593811 .. 26596798
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00103610.1

Sequence Viewer

Length: 2988 bp
ATGGCTGAAACAATCGTTAATCTACTCCTGGATCGTTTGGCTTCGGTCACCACTGACAAGATCGGAAGAGAGCTGAAGCTGGTGATGGGTGTTGATGATGAAGTCGCAAATCTGTCCCGCAATTTGAAACTTATCAGAGCTGTGCTTCAGGACGCAGAGGAGAAACATGTCATCGAGGCCAGCATGGGAGAAGTGACAGGGGCCAGCGTGGGACACTGGCTGAATGAACTGAACCAAGTTTCTCATGAGATGGACGACGTGTTGGATGAGTGGAGCACAGAGATTCTTAAGCAGCAATTACTGGCGGATGAACAAGAAGGTTTGAACTCAACGGAGGCCTTGGAGATTCCGGAGGGTTCAAACAAAGGGGAAATTATTGAAACTGCTCCTCGTACTAAGGAGAAGGTATGTCTCCCCCTTCCTTATTGCTTTTCTTGCTGCCGGGTCAAACAAGTAGTTCAGCGTCCAGTCAATGCAATCATTCGAGTAGTTCATCGTCGCGACATTGCTGTCAAAATACAACGTCTAGACGAAAGGTTAACTCAGATTGCTAATGAAAGACAGCGTTATAGCTTTCAAGATACAAAAGAAGCACCTAAACCACCTCCACGACAACAAACTTCTTCTCTTGTCATTGACAAATCCTTTGGTCGAAAAACCGAAAAGGACCTTTTAGTAGTCAAGTTGCTAGATGAGAGTAGCAGTCATAAGAGGAGGGGTCCTCTTATCATCCCTATAATAGGGTTGGGAGGGATAGGGAAAACAACTCTCTGTCAACTAGTCTATAATGATGACAAGGTCAAGGCCCATTTTGAGAAGAGGATATGGGTCTGTGTCTCGGACCCTTTTGAAGTGATTACGATTGCTAAAGCCATCATAGAAAGTCTTGGTAATGTGAGCAATACTCCAAACTCAAATCAGTTAGACCCTTTAATGGTACGCATAGAAGAATTAATCATGGGAGAAAAATTTCTCCTCGTCCTAGATGATGTGTGGAACCCAAATTCTAGTCAATGGAATGAATTAATCAAACCCTTGCTTAAGGGTGCCACTGGCAGTAGAATATTGGTAACCACACGAAAAGAGGAGGTTGCTCAAGTAATGGGAGCGACCAATCAAATGACTCACTTGAAGGCATTAAGTGAAGAGTGCACTCGGTTGCTGTTCAATCACATTACCTTCCTTGACAGGGAAGATGAGTTTGAAAAGTTTGAAATGTTTGTTCAAGAGTTTGTAGAGAAGTGTAAAGGTTTACCTCTTGTTGCGAAGACTTTAGGTAGTCTCATGCGCCATAAGAAAACAAAAAAAGAATGGCAAGATGTATTAAAAAGTCCCATATGGGAATTAAAAAGATTTCCCGAGGAGGTTTTTCAACCATTATTGTTAAGTTATTATGATTTGCCTCCAACCATAAGGCGTTGTCTTTTATATTGCGTTACTTTTCCGAAAGATTATGTGATTGATAAAGATGTTTTGATTGAGTTATGGATGTCACAAAACTATTTGTACAAGAAGCAAAAGAAAGAAGAAGAGGTTGGTCAAACATATTTTGAGGATTTAGCAATGAGGTCTTTCTTCCAAGATTTTGTGATAGATGATGATGGGAATGTTACAAAATGCAAAATGCACGATATTATGCATGACCTTTTGCAGTACTTGACTGCGGAAGAATGTTTTAGTATGGAGGTGAAGGGTGCTGATGAGATGATGGAGCTTCCCAACGGTAACAGTTTTCGTCACTTAACCCTGATGTTTGCATCTCAGGGTTTATCTAGCATCCCTTCTTCTCGAAATTGTGGAAATTTGCGTACCTTCACTACTTTTGCTTCAAGAATTTATAGAATTGAGCCAAAGTTTGTAACTTCGCTGAAACGTGTTAGGACTTTGAATTTGAGTCAGACCCTCCTCGAAGTAGTTCCAAAAGAGGTTGGTGAATTGCTACATTTGAGGTATCTTGATCTATCACATAATTTGTTTAAGGAATTACCAGACGAAATAGGTGATTTGTACAATCTACAAACTTTGCGGCTTGTTCGGTGCCGTAATCTGGTAAAATTGCCTGATATGAGAAAGTTAATCAATTTGAAGCATCTTTACGTTAAGGATTGTTTTCAGCATCGAAAGTCAAAAGCTATTGGTAGATTGAAATGGCTGCAAACACTAGATGAGTTTCACGTGAGCGAAGAGACCAGAAAAGAAGGATTGCAGTTGGAGGATCTGGAAAACTTGGACAAGCTTCAAGGGGCTCTTTCCGTGCAACACGTGGCATCGTCATTGCAAACTGTGAGTGGGGCCGTGAGAGCACGGTTGGATAAGAAACGCCTCCTTCACTTGGAACTTAATTTCGATTGTTCCTGTGGAAGCAGCACCATGCGGCCGGCCAAAGCCGAAGGAGCGATACTGAATGCTTTAAAACCACATCCAGATTTGGAATCATTAAGCATCCTTGGGTGTTTTGGGGTTACCTCGACTCCCACTTGGCTCATGTCTTTACCAAACTTGAAGAGGCTTAATCTTTCCCTCGTGCATCTTGAGGTTTTACCTGCTTTGGGGACACTGTCGTTCCTTGAATCGCTGGATATATATAAGAATTATACACTGCAAAAGGTGGGGGTTGAGTTTCTGGGGATAAATCGAATATCATCATCATCGTCATCGGAGGTTATTTCATTCCCAAAGTTAAAACACCTCAGATTCGGTTCCATCTATGAATGGGAAGAGTGGGAAGAAGTTGATTCTCAGAATATCAGGATAATGCCATGCCTTTCATCCTTAGAAGTGAAGCATTGTCAAAAACTAAAAGCTCTGCCGGAGTTCCTATGGAAGACACCACTGAAGAGTTTGACCATTGCGGATAGTCCAATTCTGCATTCTGTGTACTATGGAGGCAACAATATTCGGTGGACCACTTATGGTGAGGAGACAGCCCCGACAAATGAGGGGTTTAACAAGATATCTCACATCCAAAACATCATATTTGATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

995

Amino Acids

113.87

Weight (kDa)

6.38

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 103 4e-17 Rx N-terminal domain
NB-ARC PF00931 236 - 394 1.6e-32 NB-ARC domain
WHD_DRP PF23559 481 - 549 8.8e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 602 - 928 6.7e-29 Leucine-rich repeat region
LRR_8 PF13855 610 - 658 3.1e-06 Leucine rich repeat
LRR_8 PF13855 625 - 680 3.2e-07 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 737 - 862 4e-19 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022652)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0142201
rosa_multiflora Rmu_sc0001211.1_g000061
rosa_roxburghii Rroxscaffold_2G00103610
rosa_rugosa Rorug02G0371100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 2551
AccB1I GGYRCC 2 cut(s) 1046, 2037
AccII CGCG 1 cut(s) 501
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 6 cut(s) 118, 305, 1664, 2026, 2374, 2852
AclWI GGATC 2 cut(s) 39, 2223
AcoI YGGCCR 2 cut(s) 2375, 2379
AcsI RAATTY 5 cut(s) 968, 1003, 1801, 1833, 1888
AcuI CTGAAG 3 cut(s) 95, 131, 2855
AcvI CACGTG 2 cut(s) 2176, 2263
AfaI GTAC 7 cut(s) 394, 939, 1508, 1655, 1810, 2009, 2879
AfiI CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2332, 2549
AflII CTTAAG 2 cut(s) 287, 1040
AflIII ACRYGT 4 cut(s) 166, 258, 1873, 2260
AhlI ACTAGT 1 cut(s) 778
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 4 cut(s) 1518, 1550, 2854, 2886
AluBI AGCT 8 cut(s) 73, 79, 140, 573, 1714, 2132, 2236, 2804
AluI AGCT 8 cut(s) 73, 79, 140, 573, 1714, 2132, 2236, 2804
Alw21I GWGCWC 3 cut(s) 278, 1154, 2305
Alw26I GTCTC 5 cut(s) 416, 841, 1286, 2180, 2915
Alw44I GTGCAC 1 cut(s) 1150
AlwI GGATC 2 cut(s) 39, 2223
Ama87I CYCGRG 1 cut(s) 1358
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 7 cut(s) 177, 201, 336, 804, 2292, 2375, 2379
ApaLI GTGCAC 1 cut(s) 1150
ApeKI GCWGC 4 cut(s) 292, 438, 2152, 2364
ApoI RAATTY 5 cut(s) 968, 1003, 1801, 1833, 1888
ArsI GACNNNNNNTTYG 4 cut(s) 629, 661, 1873, 1905
AseI ATTAAT 2 cut(s) 953, 1025
Asp700I GAANNNNTTC 2 cut(s) 969, 1914
AspLEI GCGC 1 cut(s) 1290
AspS9I GGNCC 7 cut(s) 201, 667, 719, 805, 841, 2292, 2904
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 6 cut(s) 40, 94, 1699, 1943, 2012, 2927
AvaI CYCGRG 1 cut(s) 1358
AvaII GGWCC 4 cut(s) 667, 719, 841, 2904
BaeGI GKGCMC 1 cut(s) 1154
BanI GGYRCC 2 cut(s) 1046, 2037
BanII GRGCYC 1 cut(s) 2248
BarI GAAGNNNNNNTAC 4 cut(s) 2078, 2110, 2382, 2414
BauI CACGAG 1 cut(s) 2522
BbrPI CACGTG 2 cut(s) 2176, 2263
BbsI GAAGAC 2 cut(s) 1274, 2831
Bbv12I GWGCWC 3 cut(s) 278, 1154, 2305
BbvI GCAGC 4 cut(s) 304, 425, 2139, 2376
BccI CCATC 6 cut(s) 79, 244, 881, 1595, 1702, 2711
BceAI ACGGC 2 cut(s) 2025, 2279
BciT130I CCWGG 1 cut(s) 29
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 5 cut(s) 416, 841, 1286, 2180, 2915
BcuI ACTAGT 1 cut(s) 778
BfaI CTAG 7 cut(s) 527, 689, 779, 983, 1008, 1773, 2162
BfrI CTTAAG 2 cut(s) 287, 1040
BfuAI ACCTGC 1 cut(s) 2551
BisI GCNGC 6 cut(s) 293, 439, 2027, 2153, 2365, 2375
BlsI GCNGC 6 cut(s) 294, 440, 2028, 2154, 2366, 2376
BmcAI AGTACT 1 cut(s) 1655
Bme1390I CCNGG 2 cut(s) 29, 443
Bme18I GGWCC 4 cut(s) 667, 719, 841, 2904
BmeT110I CYCGRG 1 cut(s) 1358
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 7 cut(s) 201, 667, 719, 805, 841, 2292, 2904
BmiI GGNNCC 8 cut(s) 202, 720, 843, 998, 1048, 2039, 2293, 2701
BmrFI CCNGG 2 cut(s) 29, 443
BmsI GCATC 7 cut(s) 1766, 1785, 2098, 2125, 2276, 2451, 2536
BpiI GAAGAC 2 cut(s) 1274, 2831
BplI GAGNNNNNCTC 4 cut(s) 706, 738, 889, 921
BpuEI CTTGAG 2 cut(s) 1080, 2552
BpuMI CCSGG 1 cut(s) 443
BsaAI YACGTR 2 cut(s) 2176, 2263
BsaI GGTCTC 1 cut(s) 2180
BsaJI CCNNGG 3 cut(s) 339, 1359, 2446
BsaWI WCCGGW 1 cut(s) 349
BsaXI ACNNNNNCTCC 2 cut(s) 2455, 2485
Bsc4I CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2332, 2549
Bse118I RCCGGY 1 cut(s) 2377
Bse1I ACTGG 4 cut(s) 221, 306, 467, 1057
Bse3DI GCAATG 4 cut(s) 504, 1569, 2273, 2847
BseAI TCCGGA 1 cut(s) 349
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 339, 1359, 2446
BseGI GGATG 9 cut(s) 271, 313, 729, 1494, 1776, 2419, 2442, 2768, 2961
BseLI CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2332, 2549
BseMI GCAATG 4 cut(s) 504, 1569, 2273, 2847
BseMII CTCAG 4 cut(s) 557, 1775, 2704, 2753
BseNI ACTGG 4 cut(s) 221, 306, 467, 1057
BseRI GAGGAG 8 cut(s) 173, 378, 727, 965, 1100, 1376, 1895, 2933
BseSI GKGCMC 1 cut(s) 1154
BseX3I CGGCCG 1 cut(s) 2375
BseXI GCAGC 4 cut(s) 304, 425, 2139, 2376
Bsh1236I CGCG 1 cut(s) 501
Bsh1285I CGRYCG 1 cut(s) 2378
BshFI GGCC 7 cut(s) 179, 203, 338, 806, 2294, 2377, 2381
BshNI GGYRCC 2 cut(s) 1046, 2037
BsiEI CGRYCG 1 cut(s) 2378
BsiHKAI GWGCWC 3 cut(s) 278, 1154, 2305
BsiHKCI CYCGRG 1 cut(s) 1358
BsiSI CCGG 4 cut(s) 350, 442, 2378, 2810
BslFI GGGAC 4 cut(s) 100, 225, 1317, 2566
BslI CCNNNNNNNGG 6 cut(s) 740, 934, 1189, 2047, 2332, 2549
BsmAI GTCTC 5 cut(s) 416, 841, 1286, 2180, 2915
BsmFI GGGAC 4 cut(s) 100, 225, 1317, 2566
BsmI GAATGC 2 cut(s) 2410, 2869
BsnI GGCC 7 cut(s) 179, 203, 338, 806, 2294, 2377, 2381
Bso31I GGTCTC 1 cut(s) 2180
BsoBI CYCGRG 1 cut(s) 1358
Bsp1286I GDGCHC 4 cut(s) 278, 1154, 2248, 2305
Bsp13I TCCGGA 1 cut(s) 349
Bsp1407I TGTACA 2 cut(s) 1506, 2007
Bsp143I GATC 4 cut(s) 31, 60, 1957, 2215
Bsp68I TCGCGA 1 cut(s) 501
BspACI CCGC 6 cut(s) 118, 305, 1664, 2026, 2374, 2852
BspANI GGCC 7 cut(s) 179, 203, 338, 806, 2294, 2377, 2381
BspCNI CTCAG 4 cut(s) 556, 1774, 2703, 2752
BspEI TCCGGA 1 cut(s) 349
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 8 cut(s) 202, 720, 843, 998, 1048, 2039, 2293, 2701
BspMI ACCTGC 1 cut(s) 2551
BspPI GGATC 2 cut(s) 39, 2223
BspT107I GGYRCC 2 cut(s) 1046, 2037
BspTI CTTAAG 2 cut(s) 287, 1040
BspTNI GGTCTC 1 cut(s) 2180
BsrDI GCAATG 4 cut(s) 504, 1569, 2273, 2847
BsrFI RCCGGY 1 cut(s) 2377
BsrGI TGTACA 2 cut(s) 1506, 2007
BsrI ACTGG 4 cut(s) 221, 306, 467, 1057
BssAI RCCGGY 1 cut(s) 2377
BssECI CCNNGG 3 cut(s) 339, 1359, 2446
BssMI GATC 4 cut(s) 31, 60, 1957, 2215
BssSI CACGAG 1 cut(s) 2522
BssT1I CCWWGG 2 cut(s) 339, 2446
Bst2BI CACGAG 1 cut(s) 2522
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 5 cut(s) 1724, 1730, 2284, 2307, 2559
Bst6I CTCTTC 8 cut(s) 61, 812, 1140, 1524, 2178, 2498, 2712, 2831
BstAFI CTTAAG 2 cut(s) 287, 1040
BstAUI TGTACA 2 cut(s) 1506, 2007
BstBAI YACGTR 2 cut(s) 2176, 2263
BstC8I GCNNGC 3 cut(s) 181, 205, 2379
BstDEI CTNAG 6 cut(s) 396, 543, 1761, 2690, 2739, 2773
BstEII GGTNACC 3 cut(s) 46, 1069, 2461
BstENI CCTNNNNNAGG 1 cut(s) 738
BstF5I GGATG 9 cut(s) 271, 313, 729, 1494, 1776, 2419, 2442, 2768, 2961
BstFNI CGCG 1 cut(s) 501
BstHHI GCGC 1 cut(s) 1290
BstKTI GATC 4 cut(s) 34, 63, 1960, 2218
BstMAI GTCTC 5 cut(s) 416, 841, 1286, 2180, 2915
BstMBI GATC 4 cut(s) 31, 60, 1957, 2215
BstMCI CGRYCG 1 cut(s) 2378
BstMWI GCNNNNNNNGC 3 cut(s) 435, 2300, 2393
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 170
BstPI GGTNACC 3 cut(s) 46, 1069, 2461
BstSCI CCNGG 2 cut(s) 27, 441
BstSLI GKGCMC 1 cut(s) 1154
BstUI CGCG 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 304, 425, 2139, 2376
BstV2I GAAGAC 2 cut(s) 1274, 2831
BstX2I RGATCY 1 cut(s) 2215
BstYI RGATCY 1 cut(s) 2215
BstZI CGGCCG 1 cut(s) 2375
BsuRI GGCC 7 cut(s) 179, 203, 338, 806, 2294, 2377, 2381
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 9 cut(s) 271, 313, 729, 1494, 1776, 2419, 2442, 2768, 2961
BtsI GCAGTG 1 cut(s) 2597
BtsIMutI CAGTG 6 cut(s) 51, 214, 1050, 2555, 2597, 2831
BtuMI TCGCGA 1 cut(s) 501
BveI ACCTGC 1 cut(s) 2551
Cac8I GCNNGC 3 cut(s) 181, 205, 2379
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 1290
Cfr10I RCCGGY 1 cut(s) 2377
Cfr13I GGNCC 7 cut(s) 201, 667, 719, 805, 841, 2292, 2904
CseI GACGC 2 cut(s) 161, 452
Csp6I GTAC 7 cut(s) 393, 938, 1507, 1654, 1809, 2008, 2878
CviAII CATG 9 cut(s) 167, 184, 245, 958, 1285, 1640, 2371, 2485, 2760
CviQI GTAC 7 cut(s) 393, 938, 1507, 1654, 1809, 2008, 2878
DdeI CTNAG 6 cut(s) 396, 543, 1761, 2690, 2739, 2773
DpnI GATC 4 cut(s) 33, 62, 1959, 2217
DpnII GATC 4 cut(s) 31, 60, 1957, 2215
DraI TTTAAA 1 cut(s) 2412
DrdI GACNNNNNNGTC 1 cut(s) 509
DseDI GACNNNNNNGTC 1 cut(s) 509
EaeI YGGCCR 2 cut(s) 2375, 2379
EagI CGGCCG 1 cut(s) 2375
Eam1104I CTCTTC 8 cut(s) 61, 812, 1140, 1524, 2178, 2498, 2712, 2831
EarI CTCTTC 8 cut(s) 61, 812, 1140, 1524, 2178, 2498, 2712, 2831
EciI GGCGGA 1 cut(s) 320
EclXI CGGCCG 1 cut(s) 2375
Eco130I CCWWGG 2 cut(s) 339, 2446
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 2248
Eco31I GGTCTC 1 cut(s) 2180
Eco32I GATATC 1 cut(s) 2955
Eco47I GGWCC 4 cut(s) 667, 719, 841, 2904
Eco52I CGGCCG 1 cut(s) 2375
Eco57I CTGAAG 3 cut(s) 95, 131, 2855
Eco72I CACGTG 2 cut(s) 2176, 2263
Eco88I CYCGRG 1 cut(s) 1358
Eco91I GGTNACC 3 cut(s) 46, 1069, 2461
EcoNI CCTNNNNNAGG 1 cut(s) 738
EcoO109I RGGNCCY 2 cut(s) 667, 719
EcoO65I GGTNACC 3 cut(s) 46, 1069, 2461
EcoRII CCWGG 1 cut(s) 27
EcoRV GATATC 1 cut(s) 2955
EcoT14I CCWWGG 2 cut(s) 339, 2446
EcoT22I ATGCAT 1 cut(s) 1641
EcoT38I GRGCYC 1 cut(s) 2248
ErhI CCWWGG 2 cut(s) 339, 2446
FaeI CATG 9 cut(s) 170, 187, 248, 961, 1288, 1643, 2374, 2488, 2763
FalI AAGNNNNNCTT 2 cut(s) 2232, 2264
FaqI GGGAC 4 cut(s) 100, 225, 1317, 2566
FatI CATG 9 cut(s) 166, 183, 244, 957, 1284, 1639, 2370, 2484, 2759
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 1337
Fnu4HI GCNGC 6 cut(s) 293, 439, 2027, 2153, 2365, 2375
FokI GGATG 9 cut(s) 278, 320, 716, 1501, 1763, 2406, 2429, 2755, 2948
FriOI GRGCYC 1 cut(s) 2248
FseI GGCCGGCC 1 cut(s) 2381
Fsp4HI GCNGC 6 cut(s) 293, 439, 2027, 2153, 2365, 2375
FspBI CTAG 7 cut(s) 527, 689, 779, 983, 1008, 1773, 2162
GlaI GCGC 1 cut(s) 1289
GluI GCNGC 6 cut(s) 293, 439, 2027, 2153, 2365, 2375
HaeIII GGCC 7 cut(s) 179, 203, 338, 806, 2294, 2377, 2381
HapII CCGG 4 cut(s) 350, 442, 2378, 2810
HgaI GACGC 2 cut(s) 161, 452
HhaI GCGC 1 cut(s) 1290
Hin1II CATG 9 cut(s) 170, 187, 248, 961, 1288, 1643, 2374, 2488, 2763
Hin6I GCGC 1 cut(s) 1288
HinP1I GCGC 1 cut(s) 1288
HincII GTYRAC 2 cut(s) 540, 776
HindII GTYRAC 2 cut(s) 540, 776
HindIII AAGCTT 1 cut(s) 2234
HinfI GANTC 9 cut(s) 283, 346, 1123, 1894, 2432, 2470, 2570, 2694, 2735
HpaI GTTAAC 1 cut(s) 540
HpaII CCGG 4 cut(s) 350, 442, 2378, 2810
HphI GGTGA 6 cut(s) 40, 94, 1699, 1943, 2012, 2927
Hpy166II GTNNAC 6 cut(s) 540, 776, 1152, 1253, 2878, 2904
Hpy188I TCNGA 9 cut(s) 65, 137, 546, 841, 1446, 1899, 2659, 2693, 2742
Hpy8I GTNNAC 6 cut(s) 540, 776, 1152, 1253, 2878, 2904
Hpy99I CGWCG 2 cut(s) 260, 501
HpyCH4III ACNGT 5 cut(s) 1724, 1730, 2284, 2307, 2559
HpyCH4IV ACGT 6 cut(s) 258, 523, 1873, 2097, 2175, 2262
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 2300, 2393
HpyF3I CTNAG 6 cut(s) 396, 543, 1761, 2690, 2739, 2773
HpySE526I ACGT 6 cut(s) 258, 523, 1873, 2097, 2175, 2262
Hsp92II CATG 9 cut(s) 170, 187, 248, 961, 1288, 1643, 2374, 2488, 2763
HspAI GCGC 1 cut(s) 1288
Kpn2I TCCGGA 1 cut(s) 349
KroI GCCGGC 1 cut(s) 2377
KroNI GCCGGC 1 cut(s) 2379
KspAI GTTAAC 1 cut(s) 540
Kzo9I GATC 4 cut(s) 31, 60, 1957, 2215
LmnI GCTCC 5 cut(s) 273, 391, 1106, 1711, 2393
Lsp1109I GCAGC 4 cut(s) 304, 425, 2139, 2376
LweI GCATC 7 cut(s) 1766, 1785, 2098, 2125, 2276, 2451, 2536
MaeI CTAG 7 cut(s) 527, 689, 779, 983, 1008, 1773, 2162
MaeII ACGT 6 cut(s) 258, 523, 1873, 2097, 2175, 2262
MalI GATC 4 cut(s) 33, 62, 1959, 2217
MboI GATC 4 cut(s) 31, 60, 1957, 2215
MflI RGATCY 1 cut(s) 2215
MhlI GDGCHC 4 cut(s) 278, 1154, 2248, 2305
MlyI GAGTC 3 cut(s) 1117, 1903, 2464
MmeI TCCRAC 4 cut(s) 243, 1430, 2190, 2289
Mph1103I ATGCAT 1 cut(s) 1641
MroI TCCGGA 1 cut(s) 349
MroNI GCCGGC 1 cut(s) 2377
MroXI GAANNNNTTC 2 cut(s) 969, 1914
MspCI CTTAAG 2 cut(s) 287, 1040
MspI CCGG 4 cut(s) 350, 442, 2378, 2810
MspR9I CCNGG 2 cut(s) 29, 443
Mva1269I GAATGC 2 cut(s) 2410, 2869
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 3 cut(s) 435, 2300, 2393
NaeI GCCGGC 1 cut(s) 2379
NciI CCSGG 1 cut(s) 443
NdeI CATATG 1 cut(s) 1337
NdeII GATC 4 cut(s) 31, 60, 1957, 2215
NgoMIV GCCGGC 1 cut(s) 2377
NlaIII CATG 9 cut(s) 170, 187, 248, 961, 1288, 1643, 2374, 2488, 2763
NlaIV GGNNCC 8 cut(s) 202, 720, 843, 998, 1048, 2039, 2293, 2701
NmuCI GTSAC 4 cut(s) 46, 193, 1491, 1736
NruI TCGCGA 1 cut(s) 501
NsiI ATGCAT 1 cut(s) 1641
NspI RCATGY 1 cut(s) 170
PagI TCATGA 1 cut(s) 244
PceI AGGCCT 1 cut(s) 338
PciI ACATGT 1 cut(s) 166
PctI GAATGC 2 cut(s) 2410, 2869
PdiI GCCGGC 1 cut(s) 2379
PdmI GAANNNNTTC 2 cut(s) 969, 1914
PfeI GAWTC 6 cut(s) 283, 346, 2432, 2570, 2694, 2735
PflFI GACNNNGTC 2 cut(s) 797, 2557
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 6 cut(s) 294, 440, 2028, 2154, 2366, 2376
PleI GAGTC 3 cut(s) 1117, 1902, 2464
PmaCI CACGTG 2 cut(s) 2176, 2263
PmlI CACGTG 2 cut(s) 2176, 2263
PpsI GAGTC 3 cut(s) 1117, 1902, 2464
Ppu21I YACGTR 2 cut(s) 2176, 2263
PpuMI RGGWCCY 2 cut(s) 667, 719
PscI ACATGT 1 cut(s) 166
PshBI ATTAAT 2 cut(s) 953, 1025
Psp5II RGGWCCY 2 cut(s) 667, 719
Psp6I CCWGG 1 cut(s) 27
PspCI CACGTG 2 cut(s) 2176, 2263
PspEI GGTNACC 3 cut(s) 46, 1069, 2461
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 8 cut(s) 202, 720, 843, 998, 1048, 2039, 2293, 2701
PspPI GGNCC 7 cut(s) 201, 667, 719, 805, 841, 2292, 2904
PspPPI RGGWCCY 2 cut(s) 667, 719
PsuI RGATCY 1 cut(s) 2215
PsyI GACNNNGTC 2 cut(s) 797, 2557
RigI GGCCGGCC 1 cut(s) 2381
RruI TCGCGA 1 cut(s) 501
RsaI GTAC 7 cut(s) 394, 939, 1508, 1655, 1810, 2009, 2879
RsaNI GTAC 7 cut(s) 393, 938, 1507, 1654, 1809, 2008, 2878
SatI GCNGC 6 cut(s) 293, 439, 2027, 2153, 2365, 2375
Sau3AI GATC 4 cut(s) 31, 60, 1957, 2215
Sau96I GGNCC 7 cut(s) 201, 667, 719, 805, 841, 2292, 2904
ScaI AGTACT 1 cut(s) 1655
SchI GAGTC 3 cut(s) 1117, 1903, 2464
ScrFI CCNGG 2 cut(s) 29, 443
SduI GDGCHC 4 cut(s) 278, 1154, 2248, 2305
SfaNI GCATC 7 cut(s) 1766, 1785, 2098, 2125, 2276, 2451, 2536
SinI GGWCC 4 cut(s) 667, 719, 841, 2904
SmlI CTYRAG 4 cut(s) 287, 1040, 1095, 2531
SmoI CTYRAG 4 cut(s) 287, 1040, 1095, 2531
SpeI ACTAGT 1 cut(s) 778
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 6 cut(s) 118, 305, 1664, 2026, 2374, 2852
SspI AATATT 2 cut(s) 1065, 2896
SspMI CTAG 7 cut(s) 527, 689, 779, 983, 1008, 1773, 2162
StuI AGGCCT 1 cut(s) 338
StyD4I CCNGG 2 cut(s) 27, 441
StyI CCWWGG 2 cut(s) 339, 2446
TaaI ACNGT 5 cut(s) 1724, 1730, 2284, 2307, 2559
TaiI ACGT 6 cut(s) 261, 526, 1876, 2100, 2178, 2265
TaqI TCGA 9 cut(s) 174, 484, 652, 1789, 1908, 2119, 2346, 2468, 2635
TaqII GACCGA 1 cut(s) 34
TatI WGTACW 4 cut(s) 1506, 1653, 2007, 2877
TauI GCSGC 2 cut(s) 2029, 2377
TfiI GAWTC 6 cut(s) 283, 346, 2432, 2570, 2694, 2735
TscAI CASTG 6 cut(s) 58, 221, 1057, 2562, 2604, 2838
TseFI GTSAC 4 cut(s) 46, 193, 1491, 1736
TseI GCWGC 4 cut(s) 292, 438, 2152, 2364
Tsp45I GTSAC 4 cut(s) 46, 193, 1491, 1736
TspDTI ATGAA 9 cut(s) 114, 240, 324, 482, 570, 1035, 2658, 2724, 2757
TspGWI ACGGA 2 cut(s) 347, 2242
TspRI CASTG 6 cut(s) 58, 221, 1057, 2562, 2604, 2838
Tth111I GACNNNGTC 2 cut(s) 797, 2557
Vha464I CTTAAG 2 cut(s) 287, 1040
VneI GTGCAC 1 cut(s) 1150
VpaK11BI GGWCC 4 cut(s) 667, 719, 841, 2904
VspI ATTAAT 2 cut(s) 953, 1025
XagI CCTNNNNNAGG 1 cut(s) 738
XapI RAATTY 5 cut(s) 968, 1003, 1801, 1833, 1888
XbaI TCTAGA 1 cut(s) 526
XceI RCATGY 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 969, 1914
XspI CTAG 7 cut(s) 527, 689, 779, 983, 1008, 1773, 2162
ZrmI AGTACT 1 cut(s) 1655
Zsp2I ATGCAT 1 cut(s) 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.