RchiOBHm_Chr2g0162451
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0024801
Physical Location & Seq
Reverse (-)
78183577 .. 78184082
506 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53073

Sequence Viewer

Length: 378 bp
ATGGCTTGCGCCGCTAAAATCACAATAAAAGCGATACTAGTGGGATATAAGGGTGGGTTTGATAACGTGGCGACACTGGTGGATGTTGGTGGTGGGACTGGAGGCGCAGTGGCGGAGATTGTGAAGTTGTATCCCAGCATTAAGGGCATCAACTTTGATCTACCACATGTAGTTGCAACAGCGCCGGTATACCATGGGGTGTCACATGTTGGATCGAGTGATTGCGTCAAGATATTGAAGAATTGTCGAAAGGCAATACTGGAGAGCAGTGGCAAGGTGATTATTTTGGACGTTGTTCTAGAGCCAAATGGTGATGGCATGTTCGACGACACAGGCTTGACAAAGGACAATGGTGATGGCCCTATATTTTCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

125

Amino Acids

12.87

Weight (kDa)

5.82

Isoelectric Point (pI)

21.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 5 - 64 2e-16 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0024801)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0162451
rosa_rugosa Rorug02G0498000
rosa_samantha Rh2BG576500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 189
AciI CCGC 2 cut(s) 12, 113
AclWI GGATC 1 cut(s) 220
AflIII ACRYGT 2 cut(s) 166, 205
AgsI TTSAA 2 cut(s) 238, 372
AhlI ACTAGT 1 cut(s) 37
AlwI GGATC 1 cut(s) 220
AoxI GGCC 1 cut(s) 358
AspLEI GCGC 3 cut(s) 11, 107, 184
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 3 cut(s) 289, 323, 365
BccI CCATC 2 cut(s) 308, 350
BciVI GTATCC 1 cut(s) 141
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 2 cut(s) 38, 299
BfoI RGCGCY 1 cut(s) 185
BfuI GTATCC 1 cut(s) 141
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 359
BmsI GCATC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 120, 281
BsaJI CCNNGG 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 3 cut(s) 81, 103, 264
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 1 cut(s) 88
BseNI ACTGG 3 cut(s) 81, 103, 264
BseYI CCCAGC 1 cut(s) 134
BshFI GGCC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 1 cut(s) 360
Bsp143I GATC 2 cut(s) 157, 212
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 2 cut(s) 12, 113
BspANI GGCC 1 cut(s) 360
BspPI GGATC 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 3 cut(s) 81, 103, 264
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 2 cut(s) 157, 212
BssNAI GTATAC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 193
Bst1107I GTATAC 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 7
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 3 cut(s) 11, 107, 184
BstKTI GATC 2 cut(s) 160, 215
BstMBI GATC 2 cut(s) 157, 212
BstMWI GCNNNNNNNGC 2 cut(s) 11, 144
BstNSI RCATGY 3 cut(s) 170, 209, 322
BstZ17I GTATAC 1 cut(s) 190
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 88
BtsI GCAGTG 2 cut(s) 114, 274
BtsIMutI CAGTG 3 cut(s) 74, 114, 274
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 3 cut(s) 11, 107, 184
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 1 cut(s) 359
CseI GACGC 1 cut(s) 214
CviAII CATG 4 cut(s) 167, 194, 206, 319
CviJI RGCY 4 cut(s) 5, 304, 336, 360
CviKI_1 RGCY 4 cut(s) 5, 304, 336, 360
DpnI GATC 2 cut(s) 159, 214
DpnII GATC 2 cut(s) 157, 212
EciI GGCGGA 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 4 cut(s) 170, 197, 209, 322
FaiI YATR 8 cut(s) 48, 168, 190, 195, 207, 320, 365, 376
FaqI GGGAC 1 cut(s) 109
FatI CATG 4 cut(s) 166, 193, 205, 318
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 2 cut(s) 38, 299
GlaI GCGC 3 cut(s) 10, 106, 183
GluI GCNGC 1 cut(s) 12
GsaI CCCAGC 1 cut(s) 138
GsuI CTGGAG 2 cut(s) 120, 281
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 1 cut(s) 360
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 214
HhaI GCGC 3 cut(s) 11, 107, 184
Hin1II CATG 4 cut(s) 170, 197, 209, 322
Hin6I GCGC 3 cut(s) 9, 105, 182
HinP1I GCGC 3 cut(s) 9, 105, 182
HpaII CCGG 1 cut(s) 185
HphI GGTGA 3 cut(s) 289, 323, 365
Hpy166II GTNNAC 1 cut(s) 190
Hpy188III TCNNGA 2 cut(s) 229, 299
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 1 cut(s) 329
HpyCH4IV ACGT 2 cut(s) 66, 291
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 144
HpySE526I ACGT 2 cut(s) 66, 291
Hsp92II CATG 4 cut(s) 170, 197, 209, 322
HspAI GCGC 3 cut(s) 9, 105, 182
Kzo9I GATC 2 cut(s) 157, 212
LpnPI CCDG 6 cut(s) 62, 84, 148, 198, 245, 318
LweI GCATC 1 cut(s) 156
MaeI CTAG 2 cut(s) 38, 299
MaeII ACGT 2 cut(s) 66, 291
MaeIII GTNAC 1 cut(s) 201
MalI GATC 2 cut(s) 159, 214
MboI GATC 2 cut(s) 157, 212
MboII GAAGA 1 cut(s) 250
MluCI AATT 1 cut(s) 241
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 1 cut(s) 95
MseI TTAA 1 cut(s) 141
MspI CCGG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 11, 144
NcoI CCATGG 1 cut(s) 193
NdeII GATC 2 cut(s) 157, 212
NlaIII CATG 4 cut(s) 170, 197, 209, 322
NmuCI GTSAC 1 cut(s) 201
NspI RCATGY 3 cut(s) 170, 209, 322
PciI ACATGT 2 cut(s) 166, 205
PkrI GCNGC 1 cut(s) 13
PscI ACATGT 2 cut(s) 166, 205
PspFI CCCAGC 1 cut(s) 134
PspPI GGNCC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 2 cut(s) 157, 212
Sau96I GGNCC 1 cut(s) 359
SetI ASST 3 cut(s) 69, 279, 294
SfaNI GCATC 1 cut(s) 156
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 1 cut(s) 241
SsiI CCGC 2 cut(s) 12, 113
SspMI CTAG 2 cut(s) 38, 299
StyI CCWWGG 1 cut(s) 193
TaiI ACGT 2 cut(s) 69, 294
TaqI TCGA 3 cut(s) 215, 247, 324
TasI AATT 1 cut(s) 241
TauI GCSGC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 3 cut(s) 81, 114, 274
TseFI GTSAC 1 cut(s) 201
Tsp45I GTSAC 1 cut(s) 201
TspRI CASTG 3 cut(s) 81, 114, 274
XbaI TCTAGA 1 cut(s) 298
XceI RCATGY 3 cut(s) 170, 209, 322
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 2 cut(s) 38, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.