Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G10160 |
| fragaria_vesca | FvH4_6g24860 FvH4_6g24860 FvH4_6g24860 FvH4_6g24860 FvH4_6g24860 FvH4_6g24860 FvH4_6g24860 FvH4_6g24860 FvH4_6g24860 |
| malus_domestica | MD14G1081100.v1.1 |
| prunus_persica | Prupe.7G049800_v2.0.a1 Prupe.7G049800_v2.0.a1 Prupe.7G049800_v2.0.a1 |
| pyrus_communis | pycom14g07040 |
| rosa_chinensis | RchiOBHm_Chr3g0484551 |
| rosa_laevigata | RLG00000023221 |
| rosa_multiflora | Rmu_sc0000295.1_g000036 |
| rosa_roxburghii | Rroxscaffold_6G00397650 |
| rosa_rugosa | Rorug03G0211600 Rorug03G0211700 Rorug03G0211700 |
| rosa_samantha | Rh3AG262300 Rh3BG297600 Rh3CG294600 Rh3DG290900 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 287 |
| Acc36I | ACCTGC | 1 cut(s) | 1342 |
| AccB1I | GGYRCC | 2 cut(s) | 871, 946 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 910 |
| AccI | GTMKAC | 1 cut(s) | 1403 |
| AciI | CCGC | 1 cut(s) | 1563 |
| AclWI | GGATC | 1 cut(s) | 316 |
| AcuI | CTGAAG | 2 cut(s) | 906, 1482 |
| AfaI | GTAC | 3 cut(s) | 572, 1486, 1893 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 793, 910, 1596, 1729 |
| AflIII | ACRYGT | 1 cut(s) | 1487 |
| AgsI | TTSAA | 9 cut(s) | 51, 435, 664, 704, 732, 847, 1049, 1134, 1435 |
| AjiI | CACGTC | 2 cut(s) | 299, 1372 |
| AjnI | CCWGG | 4 cut(s) | 903, 1531, 1602, 1851 |
| AjuI | GAANNNNNNNTTGG | 8 cut(s) | 696, 728, 968, 1000, 1045, 1059, 1077, 1091 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 912, 944 |
| Alw21I | GWGCWC | 3 cut(s) | 104, 222, 1252 |
| Alw26I | GTCTC | 2 cut(s) | 1446, 1621 |
| AlwI | GGATC | 1 cut(s) | 316 |
| Ama87I | CYCGRG | 1 cut(s) | 103 |
| AoxI | GGCC | 2 cut(s) | 1126, 1518 |
| ApeKI | GCWGC | 3 cut(s) | 1565, 1697, 1700 |
| AseI | ATTAAT | 1 cut(s) | 1008 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 430, 525, 1187 |
| AspS9I | GGNCC | 5 cut(s) | 229, 784, 1126, 1519, 1600 |
| AsuHPI | GGTGA | 3 cut(s) | 14, 494, 1004 |
| AsuII | TTCGAA | 1 cut(s) | 1846 |
| AvaI | CYCGRG | 1 cut(s) | 103 |
| AvaII | GGWCC | 3 cut(s) | 229, 784, 1600 |
| BanI | GGYRCC | 2 cut(s) | 871, 946 |
| BanII | GRGCYC | 2 cut(s) | 104, 1252 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 444, 476, 1089, 1121, 1477, 1509 |
| BauI | CACGAG | 1 cut(s) | 915 |
| BbsI | GAAGAC | 1 cut(s) | 621 |
| Bbv12I | GWGCWC | 3 cut(s) | 104, 222, 1252 |
| BbvCI | CCTCAGC | 1 cut(s) | 1570 |
| BbvI | GCAGC | 3 cut(s) | 1552, 1684, 1712 |
| BccI | CCATC | 7 cut(s) | 66, 299, 961, 965, 1278, 1307, 1684 |
| BceAI | ACGGC | 2 cut(s) | 479, 1490 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 94, 128 |
| BciT130I | CCWGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| BclI | TGATCA | 1 cut(s) | 741 |
| BcoDI | GTCTC | 2 cut(s) | 1446, 1621 |
| BfaI | CTAG | 5 cut(s) | 150, 368, 596, 1218, 1332 |
| BfmI | CTRYAG | 4 cut(s) | 566, 1581, 1698, 1752 |
| BfuAI | ACCTGC | 1 cut(s) | 1342 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1304 |
| BglII | AGATCT | 1 cut(s) | 1225 |
| BisI | GCNGC | 3 cut(s) | 1566, 1698, 1701 |
| BlsI | GCNGC | 3 cut(s) | 1567, 1699, 1702 |
| BmcAI | AGTACT | 1 cut(s) | 572 |
| Bme1390I | CCNGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| Bme18I | GGWCC | 3 cut(s) | 229, 784, 1600 |
| BmeT110I | CYCGRG | 1 cut(s) | 103 |
| BmgBI | CACGTC | 2 cut(s) | 299, 1372 |
| BmgT120I | GGNCC | 5 cut(s) | 229, 784, 1126, 1519, 1600 |
| BmiI | GGNNCC | 6 cut(s) | 418, 873, 930, 948, 1127, 1601 |
| BmrFI | CCNGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| BmrI | ACTGGG | 1 cut(s) | 1875 |
| BmsI | GCATC | 3 cut(s) | 1635, 1684, 1723 |
| BmuI | ACTGGG | 1 cut(s) | 1875 |
| BpiI | GAAGAC | 1 cut(s) | 621 |
| BpmI | CTGGAG | 1 cut(s) | 1607 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1570 |
| Bpu14I | TTCGAA | 1 cut(s) | 1846 |
| BpuEI | CTTGAG | 4 cut(s) | 11, 162, 344, 1443 |
| BsaI | GGTCTC | 1 cut(s) | 1446 |
| BsaJI | CCNNGG | 4 cut(s) | 261, 420, 1472, 1531 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1215, 1245, 1351, 1381 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 793, 910, 1596, 1729 |
| Bse1I | ACTGG | 2 cut(s) | 1000, 1870 |
| Bse3DI | GCAATG | 2 cut(s) | 208, 897 |
| BseBI | CCWGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| BseDI | CCNNGG | 4 cut(s) | 261, 420, 1472, 1531 |
| BseGI | GGATG | 7 cut(s) | 351, 901, 976, 1669, 1699, 1738, 1894 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 793, 910, 1596, 1729 |
| BseMI | GCAATG | 2 cut(s) | 208, 897 |
| BseMII | CTCAG | 6 cut(s) | 237, 409, 994, 1251, 1584, 1828 |
| BseNI | ACTGG | 2 cut(s) | 1000, 1870 |
| BseRI | GAGGAG | 1 cut(s) | 489 |
| BseXI | GCAGC | 3 cut(s) | 1552, 1684, 1712 |
| BsgI | GTGCAG | 1 cut(s) | 1797 |
| BshFI | GGCC | 2 cut(s) | 1128, 1520 |
| BshNI | GGYRCC | 2 cut(s) | 871, 946 |
| BsiHKAI | GWGCWC | 3 cut(s) | 104, 222, 1252 |
| BsiHKCI | CYCGRG | 1 cut(s) | 103 |
| BslFI | GGGAC | 2 cut(s) | 1514, 1613 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 793, 910, 1596, 1729 |
| BsmAI | GTCTC | 2 cut(s) | 1446, 1621 |
| BsmFI | GGGAC | 2 cut(s) | 1514, 1613 |
| BsnI | GGCC | 2 cut(s) | 1128, 1520 |
| Bso31I | GGTCTC | 1 cut(s) | 1446 |
| BsoBI | CYCGRG | 1 cut(s) | 103 |
| Bsp119I | TTCGAA | 1 cut(s) | 1846 |
| Bsp1286I | GDGCHC | 3 cut(s) | 104, 222, 1252 |
| Bsp143I | GATC | 3 cut(s) | 308, 741, 1225 |
| Bsp19I | CCATGG | 1 cut(s) | 261 |
| BspACI | CCGC | 1 cut(s) | 1563 |
| BspANI | GGCC | 2 cut(s) | 1128, 1520 |
| BspCNI | CTCAG | 6 cut(s) | 236, 408, 993, 1252, 1583, 1827 |
| BspHI | TCATGA | 1 cut(s) | 1714 |
| BspLI | GGNNCC | 6 cut(s) | 418, 873, 930, 948, 1127, 1601 |
| BspMAI | CTGCAG | 3 cut(s) | 570, 1585, 1702 |
| BspMI | ACCTGC | 1 cut(s) | 1342 |
| BspPI | GGATC | 1 cut(s) | 316 |
| BspT104I | TTCGAA | 1 cut(s) | 1846 |
| BspT107I | GGYRCC | 2 cut(s) | 871, 946 |
| BspTNI | GGTCTC | 1 cut(s) | 1446 |
| BsrDI | GCAATG | 2 cut(s) | 208, 897 |
| BsrI | ACTGG | 2 cut(s) | 1000, 1870 |
| BssECI | CCNNGG | 4 cut(s) | 261, 420, 1472, 1531 |
| BssMI | GATC | 3 cut(s) | 308, 741, 1225 |
| BssSI | CACGAG | 1 cut(s) | 915 |
| BssT1I | CCWWGG | 2 cut(s) | 261, 420 |
| Bst2BI | CACGAG | 1 cut(s) | 915 |
| Bst2UI | CCWGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| Bst4CI | ACNGT | 2 cut(s) | 489, 959 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1838 |
| BstBI | TTCGAA | 1 cut(s) | 1846 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 117 |
| BstDEI | CTNAG | 7 cut(s) | 41, 223, 395, 980, 1260, 1570, 1814 |
| BstDSI | CCRYGG | 2 cut(s) | 261, 1472 |
| BstEII | GGTNACC | 1 cut(s) | 992 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1594, 1727 |
| BstF5I | GGATG | 7 cut(s) | 351, 901, 976, 1669, 1699, 1738, 1894 |
| BstKTI | GATC | 3 cut(s) | 311, 744, 1228 |
| BstMAI | GTCTC | 2 cut(s) | 1446, 1621 |
| BstMBI | GATC | 3 cut(s) | 308, 741, 1225 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 266, 869, 1019, 1304, 1474, 1571, 1580, 1838 |
| BstNI | CCWGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| BstNSI | RCATGY | 2 cut(s) | 441, 1491 |
| BstPI | GGTNACC | 1 cut(s) | 992 |
| BstSCI | CCNGG | 4 cut(s) | 903, 1531, 1602, 1851 |
| BstSFI | CTRYAG | 4 cut(s) | 566, 1581, 1698, 1752 |
| BstV1I | GCAGC | 3 cut(s) | 1552, 1684, 1712 |
| BstV2I | GAAGAC | 1 cut(s) | 621 |
| BstX2I | RGATCY | 1 cut(s) | 1225 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 836 |
| BstYI | RGATCY | 1 cut(s) | 1225 |
| BsuRI | GGCC | 2 cut(s) | 1128, 1520 |
| BtgI | CCRYGG | 2 cut(s) | 261, 1472 |
| BtrI | CACGTC | 2 cut(s) | 299, 1372 |
| BtsCI | GGATG | 7 cut(s) | 351, 901, 976, 1669, 1699, 1738, 1894 |
| BtsI | GCAGTG | 4 cut(s) | 473, 1780, 1790, 1871 |
| BtsIMutI | CAGTG | 4 cut(s) | 473, 1780, 1790, 1871 |
| BveI | ACCTGC | 1 cut(s) | 1342 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 117 |
| CciI | TCATGA | 1 cut(s) | 1714 |
| Cfr13I | GGNCC | 5 cut(s) | 229, 784, 1126, 1519, 1600 |
| Csp6I | GTAC | 3 cut(s) | 571, 1485, 1892 |
| CviQI | GTAC | 3 cut(s) | 571, 1485, 1892 |
| DdeI | CTNAG | 7 cut(s) | 41, 223, 395, 980, 1260, 1570, 1814 |
| DpnI | GATC | 3 cut(s) | 310, 743, 1227 |
| DpnII | GATC | 3 cut(s) | 308, 741, 1225 |
| Ecl136II | GAGCTC | 2 cut(s) | 102, 1250 |
| Eco130I | CCWWGG | 2 cut(s) | 261, 420 |
| Eco24I | GRGCYC | 2 cut(s) | 104, 1252 |
| Eco31I | GGTCTC | 1 cut(s) | 1446 |
| Eco47I | GGWCC | 3 cut(s) | 229, 784, 1600 |
| Eco53kI | GAGCTC | 2 cut(s) | 102, 1250 |
| Eco57I | CTGAAG | 2 cut(s) | 906, 1482 |
| Eco88I | CYCGRG | 1 cut(s) | 103 |
| Eco91I | GGTNACC | 1 cut(s) | 992 |
| EcoICRI | GAGCTC | 2 cut(s) | 102, 1250 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1594, 1727 |
| EcoO109I | RGGNCCY | 4 cut(s) | 229, 1126, 1519, 1600 |
| EcoO65I | GGTNACC | 1 cut(s) | 992 |
| EcoRII | CCWGG | 4 cut(s) | 903, 1531, 1602, 1851 |
| EcoT14I | CCWWGG | 2 cut(s) | 261, 420 |
| EcoT22I | ATGCAT | 1 cut(s) | 1650 |
| EcoT38I | GRGCYC | 2 cut(s) | 104, 1252 |
| ErhI | CCWWGG | 2 cut(s) | 261, 420 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 696, 728, 1452, 1484 |
| FaqI | GGGAC | 2 cut(s) | 1514, 1613 |
| FbaI | TGATCA | 1 cut(s) | 741 |
| FblI | GTMKAC | 1 cut(s) | 1403 |
| Fnu4HI | GCNGC | 3 cut(s) | 1566, 1698, 1701 |
| FokI | GGATG | 7 cut(s) | 358, 908, 983, 1676, 1706, 1745, 1901 |
| FriOI | GRGCYC | 2 cut(s) | 104, 1252 |
| Fsp4HI | GCNGC | 3 cut(s) | 1566, 1698, 1701 |
| FspBI | CTAG | 5 cut(s) | 150, 368, 596, 1218, 1332 |
| GluI | GCNGC | 3 cut(s) | 1566, 1698, 1701 |
| GsuI | CTGGAG | 1 cut(s) | 1607 |
| HaeIII | GGCC | 2 cut(s) | 1128, 1520 |
| HincII | GTYRAC | 1 cut(s) | 1542 |
| HindII | GTYRAC | 1 cut(s) | 1542 |
| HindIII | AAGCTT | 1 cut(s) | 52 |
| HphI | GGTGA | 3 cut(s) | 14, 494, 1004 |
| Hpy166II | GTNNAC | 6 cut(s) | 492, 680, 920, 962, 1404, 1542 |
| Hpy188I | TCNGA | 7 cut(s) | 164, 226, 398, 480, 886, 1210, 1817 |
| Hpy188III | TCNNGA | 6 cut(s) | 361, 1259, 1332, 1513, 1624, 1715 |
| Hpy8I | GTNNAC | 6 cut(s) | 492, 680, 920, 962, 1404, 1542 |
| HpyAV | CCTTC | 4 cut(s) | 1074, 1510, 1648, 1797 |
| HpyCH4III | ACNGT | 2 cut(s) | 489, 959 |
| HpyCH4IV | ACGT | 2 cut(s) | 298, 1371 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 266, 869, 1019, 1304, 1474, 1571, 1580, 1838 |
| HpyF3I | CTNAG | 7 cut(s) | 41, 223, 395, 980, 1260, 1570, 1814 |
| HpySE526I | ACGT | 2 cut(s) | 298, 1371 |
| Ksp22I | TGATCA | 1 cut(s) | 741 |
| Kzo9I | GATC | 3 cut(s) | 308, 741, 1225 |
| LmnI | GCTCC | 2 cut(s) | 1247, 1348 |
| Lsp1109I | GCAGC | 3 cut(s) | 1552, 1684, 1712 |
| LweI | GCATC | 3 cut(s) | 1635, 1684, 1723 |
| MaeI | CTAG | 5 cut(s) | 150, 368, 596, 1218, 1332 |
| MaeII | ACGT | 2 cut(s) | 298, 1371 |
| MaeIII | GTNAC | 5 cut(s) | 992, 1367, 1555, 1861, 1869 |
| MalI | GATC | 3 cut(s) | 310, 743, 1227 |
| MboI | GATC | 3 cut(s) | 308, 741, 1225 |
| MboII | GAAGA | 4 cut(s) | 566, 626, 1109, 1488 |
| MfeI | CAATTG | 1 cut(s) | 1435 |
| MflI | RGATCY | 1 cut(s) | 1225 |
| MhlI | GDGCHC | 3 cut(s) | 104, 222, 1252 |
| MlyI | GAGTC | 3 cut(s) | 115, 387, 1409 |
| MmeI | TCCRAC | 1 cut(s) | 522 |
| Mph1103I | ATGCAT | 1 cut(s) | 1650 |
| MroXI | GAANNNNTTC | 3 cut(s) | 430, 525, 1187 |
| MseI | TTAA | 3 cut(s) | 141, 1008, 1338 |
| MspA1I | CMGCKG | 1 cut(s) | 1565 |
| MspR9I | CCNGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| MunI | CAATTG | 1 cut(s) | 1435 |
| MvaI | CCWGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 266, 869, 1019, 1304, 1474, 1571, 1580, 1838 |
| NcoI | CCATGG | 1 cut(s) | 261 |
| NdeII | GATC | 3 cut(s) | 308, 741, 1225 |
| NlaIV | GGNNCC | 6 cut(s) | 418, 873, 930, 948, 1127, 1601 |
| NmuCI | GTSAC | 3 cut(s) | 992, 1367, 1869 |
| NsiI | ATGCAT | 1 cut(s) | 1650 |
| NspI | RCATGY | 2 cut(s) | 441, 1491 |
| NspV | TTCGAA | 1 cut(s) | 1846 |
| PaeR7I | CTCGAG | 1 cut(s) | 103 |
| PagI | TCATGA | 1 cut(s) | 1714 |
| PciI | ACATGT | 1 cut(s) | 1487 |
| PdmI | GAANNNNTTC | 3 cut(s) | 430, 525, 1187 |
| PfeI | GAWTC | 7 cut(s) | 159, 254, 728, 1423, 1586, 1682, 1848 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 910 |
| PfoI | TCCNGGA | 1 cut(s) | 903 |
| PkrI | GCNGC | 3 cut(s) | 1567, 1699, 1702 |
| PleI | GAGTC | 3 cut(s) | 114, 387, 1408 |
| PpsI | GAGTC | 3 cut(s) | 114, 387, 1408 |
| PpuMI | RGGWCCY | 2 cut(s) | 229, 1600 |
| PscI | ACATGT | 1 cut(s) | 1487 |
| PshBI | ATTAAT | 1 cut(s) | 1008 |
| PsiI | TTATAA | 1 cut(s) | 287 |
| Psp124BI | GAGCTC | 2 cut(s) | 104, 1252 |
| Psp5II | RGGWCCY | 2 cut(s) | 229, 1600 |
| Psp6I | CCWGG | 4 cut(s) | 903, 1531, 1602, 1851 |
| PspEI | GGTNACC | 1 cut(s) | 992 |
| PspGI | CCWGG | 4 cut(s) | 903, 1531, 1602, 1851 |
| PspN4I | GGNNCC | 6 cut(s) | 418, 873, 930, 948, 1127, 1601 |
| PspPI | GGNCC | 5 cut(s) | 229, 784, 1126, 1519, 1600 |
| PspPPI | RGGWCCY | 2 cut(s) | 229, 1600 |
| PspXI | VCTCGAGB | 1 cut(s) | 103 |
| PstI | CTGCAG | 3 cut(s) | 570, 1585, 1702 |
| PsuI | RGATCY | 1 cut(s) | 1225 |
| RsaI | GTAC | 3 cut(s) | 572, 1486, 1893 |
| RsaNI | GTAC | 3 cut(s) | 571, 1485, 1892 |
| SacI | GAGCTC | 2 cut(s) | 104, 1252 |
| SaqAI | TTAA | 3 cut(s) | 141, 1008, 1338 |
| SatI | GCNGC | 3 cut(s) | 1566, 1698, 1701 |
| Sau3AI | GATC | 3 cut(s) | 308, 741, 1225 |
| Sau96I | GGNCC | 5 cut(s) | 229, 784, 1126, 1519, 1600 |
| ScaI | AGTACT | 1 cut(s) | 572 |
| SchI | GAGTC | 3 cut(s) | 115, 387, 1409 |
| ScrFI | CCNGG | 4 cut(s) | 905, 1533, 1604, 1853 |
| SduI | GDGCHC | 3 cut(s) | 104, 222, 1252 |
| SfaNI | GCATC | 3 cut(s) | 1635, 1684, 1723 |
| SfcI | CTRYAG | 4 cut(s) | 566, 1581, 1698, 1752 |
| Sfr274I | CTCGAG | 1 cut(s) | 103 |
| SfuI | TTCGAA | 1 cut(s) | 1846 |
| SinI | GGWCC | 3 cut(s) | 229, 784, 1600 |
| SlaI | CTCGAG | 1 cut(s) | 103 |
| SmlI | CTYRAG | 5 cut(s) | 26, 103, 177, 359, 1458 |
| SmoI | CTYRAG | 5 cut(s) | 26, 103, 177, 359, 1458 |
| SsiI | CCGC | 1 cut(s) | 1563 |
| SspI | AATATT | 1 cut(s) | 843 |
| SspMI | CTAG | 5 cut(s) | 150, 368, 596, 1218, 1332 |
| SstI | GAGCTC | 2 cut(s) | 104, 1252 |
| StyD4I | CCNGG | 4 cut(s) | 903, 1531, 1602, 1851 |
| StyI | CCWWGG | 2 cut(s) | 261, 420 |
| TaaI | ACNGT | 2 cut(s) | 489, 959 |
| TaiI | ACGT | 2 cut(s) | 301, 1374 |
| TaqI | TCGA | 5 cut(s) | 104, 1181, 1514, 1617, 1846 |
| TatI | WGTACW | 3 cut(s) | 570, 1484, 1891 |
| TfiI | GAWTC | 7 cut(s) | 159, 254, 728, 1423, 1586, 1682, 1848 |
| Tru1I | TTAA | 3 cut(s) | 141, 1008, 1338 |
| Tru9I | TTAA | 3 cut(s) | 141, 1008, 1338 |
| TscAI | CASTG | 4 cut(s) | 473, 1780, 1797, 1878 |
| TseFI | GTSAC | 3 cut(s) | 992, 1367, 1869 |
| TseI | GCWGC | 3 cut(s) | 1565, 1697, 1700 |
| Tsp45I | GTSAC | 3 cut(s) | 992, 1367, 1869 |
| TspDTI | ATGAA | 5 cut(s) | 267, 345, 395, 994, 1185 |
| TspGWI | ACGGA | 2 cut(s) | 626, 1260 |
| TspRI | CASTG | 4 cut(s) | 473, 1780, 1797, 1878 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 910 |
| VpaK11BI | GGWCC | 3 cut(s) | 229, 784, 1600 |
| VspI | ATTAAT | 1 cut(s) | 1008 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1594, 1727 |
| XbaI | TCTAGA | 1 cut(s) | 1331 |
| XceI | RCATGY | 2 cut(s) | 441, 1491 |
| XhoI | CTCGAG | 1 cut(s) | 103 |
| XmiI | GTMKAC | 1 cut(s) | 1403 |
| XmnI | GAANNNNTTC | 3 cut(s) | 430, 525, 1187 |
| XspI | CTAG | 5 cut(s) | 150, 368, 596, 1218, 1332 |
| ZrmI | AGTACT | 1 cut(s) | 572 |
| Zsp2I | ATGCAT | 1 cut(s) | 1650 |