RchiOBHm_Chr4g0413271

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0019409
Physical Location & Seq
Reverse (-)
37524977 .. 37525975
999 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38380

Sequence Viewer

Length: 999 bp
ATGAACCGATGCCGGTTCGTGTGCAAGTCTTGGCTCCGCTTGTTCTCAGACCCCAGTTTCACCAAAACCCTATGTTGCAGAACACCCACCAGCCTTGTTGTTGTGTCGTCTACCCCATCTAGGGACTACTACTTGGTCGGCCTGGACGAGGCCTCCAGACCAAACGGAGATGTGTTGGAGCTGTGTAGTTATAGCAGGGAGTGCCTTGACATAATCGGTTCTTTCAATGGTTTCCTCTTCCTATATGATTCCGCGCTTATCGATGATCATCGTCTTTACTTATCCAATCCCATCACCAGAGAGCGTTTGGCCTTGCCCAAGTATGATGGTCTTAATATAGACACTAACTTGGGGTTTGGGTTTAGTCCAGCCAGCAATGTCTACAAAGTATTTGTGTTTAAAAGTGAAGAAGAAAATGGAGACTTCCAAGACGAATATAATGAAGAGGAGGTGGTGGTTTTGACGGTTGGCTCTTCGGTTTGGAGAAACATTGGGAAGTATTCAGTCTATTTTGGGAATACAGAATATGGATTTCCTTTTAATGGCTTTCTTCATTGGGTTGGGAGAGCCAATAATTTGGTATGGGGATTTGATGTAGAAAGGGAGCTTTTCCAACAGTTTCCACCACTCCCATTGACATCTGAGGATACTATTCTTCTTGACAGTAGTGATTCGAGACTTGCAGCTTTGAGAGCTTCGCTCTGCCTTATAGTTACTAGGGGCCAAAAAGTCAGTGTTTGGGTGATGAAGGATCATGGTGTTAAGGGGTCTTGGAATAAGGAGTTTGAAATTCTGAATGAGTCTTTCGATCGATATATTCCTCAGCTACTGAAATTTACAGAGGGGCAGGTCTTGTTGTTACGTGACGCTCAATTGGAAACTCATAGTCCTGGAAAAAAAGGCTTTTCCATGGTTGAGGTTGATGGGATACCAAAAAAGCTTTATAGTGGATATGTCCATATTCCAAGCTTTATTTCGCTTGCGGATATCAGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

332

Amino Acids

37.76

Weight (kDa)

5.28

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 65 - 271 2.6e-17 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 70 - 259 2.2e-07 KIB1-4 beta-propeller
FBA_1 PF07734 76 - 277 4.5e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019409)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0413271
rosa_laevigata RLG00000008226
rosa_multiflora Rmu_sc0000717.1_g000002
rosa_rugosa Rorug04G0119100
rosa_samantha Rh4AG179000 Rh4CG189800 Rh4DG174200
rosa_wichuraiana Rw4G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 838
AccI GTMKAC 2 cut(s) 110, 381
AccII CGCG 1 cut(s) 254
AciI CCGC 3 cut(s) 37, 252, 983
AclWI GGATC 1 cut(s) 759
AcsI RAATTY 2 cut(s) 789, 833
AfiI CCNNNNNNNGG 4 cut(s) 120, 121, 148, 542
AgsI TTSAA 2 cut(s) 226, 788
AjnI CCWGG 2 cut(s) 141, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 958, 990
AluBI AGCT 7 cut(s) 181, 607, 686, 695, 826, 940, 969
AluI AGCT 7 cut(s) 181, 607, 686, 695, 826, 940, 969
Alw26I GTCTC 2 cut(s) 414, 670
AlwI GGATC 1 cut(s) 759
AlwNI CAGNNNCTG 1 cut(s) 829
AoxI GGCC 4 cut(s) 139, 150, 309, 721
ApeKI GCWGC 1 cut(s) 683
ApoI RAATTY 2 cut(s) 789, 833
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 721
AsuHPI GGTGA 3 cut(s) 52, 286, 754
BbvCI CCTCAGC 1 cut(s) 822
BbvI GCAGC 1 cut(s) 695
BccI CCATC 4 cut(s) 124, 299, 320, 917
BciT130I CCWGG 2 cut(s) 143, 891
BciVI GTATCC 2 cut(s) 640, 921
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 414, 670
BfaI CTAG 2 cut(s) 120, 717
BfuAI ACCTGC 1 cut(s) 838
BfuI GTATCC 2 cut(s) 640, 921
BisI GCNGC 1 cut(s) 684
BlsI GCNGC 1 cut(s) 685
Bme1390I CCNGG 2 cut(s) 143, 891
BmgT120I GGNCC 1 cut(s) 721
BmiI GGNNCC 2 cut(s) 35, 722
BmrFI CCNGG 2 cut(s) 143, 891
BmrI ACTGGG 1 cut(s) 48
BmuI ACTGGG 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 684, 716
BpmI CTGGAG 1 cut(s) 139
Bpu10I CCTNAGC 1 cut(s) 822
Bsa29I ATCGAT 2 cut(s) 261, 811
BsaAI YACGTR 1 cut(s) 863
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 909
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 121, 148, 542
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 382
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 143, 891
BseCI ATCGAT 2 cut(s) 261, 811
BseDI CCNNGG 1 cut(s) 909
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 4 cut(s) 120, 121, 148, 542
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 3 cut(s) 60, 633, 836
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 1 cut(s) 461
BseXI GCAGC 1 cut(s) 695
Bsh1236I CGCG 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 811
BshFI GGCC 4 cut(s) 141, 152, 311, 723
BshVI ATCGAT 2 cut(s) 261, 811
BsiEI CGRYCG 1 cut(s) 811
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 4 cut(s) 120, 121, 148, 542
BsmAI GTCTC 2 cut(s) 414, 670
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 4 cut(s) 141, 152, 311, 723
Bsp143I GATC 3 cut(s) 265, 751, 808
Bsp19I CCATGG 1 cut(s) 909
BspACI CCGC 3 cut(s) 37, 252, 983
BspANI GGCC 4 cut(s) 141, 152, 311, 723
BspCNI CTCAG 3 cut(s) 59, 634, 835
BspDI ATCGAT 2 cut(s) 261, 811
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 2 cut(s) 35, 722
BspMI ACCTGC 1 cut(s) 838
BspPI GGATC 1 cut(s) 759
BspQI GCTCTTC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 12
BsrI ACTGG 1 cut(s) 54
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 909
BssMI GATC 3 cut(s) 265, 751, 808
BssT1I CCWWGG 1 cut(s) 909
Bst2UI CCWGG 2 cut(s) 143, 891
Bst4CI ACNGT 3 cut(s) 466, 618, 665
Bst6I CTCTTC 3 cut(s) 242, 438, 478
BstAPI GCANNNNNTGC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 863
BstC8I GCNNGC 2 cut(s) 373, 981
BstDEI CTNAG 3 cut(s) 46, 642, 822
BstDSI CCRYGG 1 cut(s) 909
BstENI CCTNNNNNAGG 1 cut(s) 146
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 3 cut(s) 268, 754, 811
BstMAI GTCTC 2 cut(s) 414, 670
BstMBI GATC 3 cut(s) 265, 751, 808
BstMCI CGRYCG 1 cut(s) 811
BstMWI GCNNNNNNNGC 2 cut(s) 201, 692
BstNI CCWGG 2 cut(s) 143, 891
BstSCI CCNGG 2 cut(s) 141, 889
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 695
BstXI CCANNNNNNTGG 1 cut(s) 577
Bsu15I ATCGAT 2 cut(s) 261, 811
BsuI GTATCC 2 cut(s) 640, 921
BsuRI GGCC 4 cut(s) 141, 152, 311, 723
BsuTUI ATCGAT 2 cut(s) 261, 811
BtgI CCRYGG 1 cut(s) 909
BtsIMutI CAGTG 1 cut(s) 739
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 2 cut(s) 373, 981
CaiI CAGNNNCTG 1 cut(s) 829
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 12
Cfr13I GGNCC 1 cut(s) 721
ClaI ATCGAT 2 cut(s) 261, 811
CseI GACGC 1 cut(s) 875
CspCI CAANNNNNGTGG 4 cut(s) 76, 111, 615, 650
CviAII CATG 2 cut(s) 755, 910
DdeI CTNAG 3 cut(s) 46, 642, 822
DpnI GATC 3 cut(s) 267, 753, 810
DpnII GATC 3 cut(s) 265, 751, 808
DraI TTTAAA 1 cut(s) 400
Eam1104I CTCTTC 3 cut(s) 242, 438, 478
EarI CTCTTC 3 cut(s) 242, 438, 478
Eco130I CCWWGG 1 cut(s) 909
Eco147I AGGCCT 1 cut(s) 152
Eco32I GATATC 1 cut(s) 988
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 141, 889
EcoRV GATATC 1 cut(s) 988
EcoT14I CCWWGG 1 cut(s) 909
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 2 cut(s) 758, 913
FaqI GGGAC 1 cut(s) 137
FatI CATG 2 cut(s) 754, 909
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 2 cut(s) 110, 381
Fnu4HI GCNGC 1 cut(s) 684
Fsp4HI GCNGC 1 cut(s) 684
FspBI CTAG 2 cut(s) 120, 717
GlaI GCGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 684
GsuI CTGGAG 1 cut(s) 139
HaeIII GGCC 4 cut(s) 141, 152, 311, 723
HapII CCGG 1 cut(s) 13
HgaI GACGC 1 cut(s) 875
HhaI GCGC 1 cut(s) 256
Hin1II CATG 2 cut(s) 758, 913
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HindIII AAGCTT 2 cut(s) 938, 967
HinfI GANTC 3 cut(s) 248, 671, 800
HpaII CCGG 1 cut(s) 13
HphI GGTGA 3 cut(s) 52, 286, 754
Hpy166II GTNNAC 2 cut(s) 111, 382
Hpy188I TCNGA 3 cut(s) 49, 643, 795
Hpy188III TCNNGA 3 cut(s) 156, 659, 675
Hpy8I GTNNAC 2 cut(s) 111, 382
HpyAV CCTTC 1 cut(s) 742
HpyCH4III ACNGT 3 cut(s) 466, 618, 665
HpyCH4IV ACGT 1 cut(s) 862
HpyCH4V TGCA 3 cut(s) 24, 78, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 692
HpyF3I CTNAG 3 cut(s) 46, 642, 822
HpySE526I ACGT 1 cut(s) 862
Hsp92II CATG 2 cut(s) 758, 913
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 3 cut(s) 265, 751, 808
LguI GCTCTTC 1 cut(s) 478
LmnI GCTCC 3 cut(s) 39, 178, 604
Lsp1109I GCAGC 1 cut(s) 695
MaeI CTAG 2 cut(s) 120, 717
MaeII ACGT 1 cut(s) 862
MaeIII GTNAC 3 cut(s) 712, 858, 863
MalI GATC 3 cut(s) 267, 753, 810
MboI GATC 3 cut(s) 265, 751, 808
MboII GAAGA 7 cut(s) 229, 419, 422, 455, 465, 542, 647
MfeI CAATTG 1 cut(s) 872
MluCI AATT 4 cut(s) 574, 789, 833, 872
MlyI GAGTC 1 cut(s) 809
MmeI TCCRAC 2 cut(s) 156, 637
MnlI CCTC 9 cut(s) 142, 163, 245, 439, 442, 637, 831, 835, 910
MseI TTAA 5 cut(s) 333, 399, 540, 762, 997
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 2 cut(s) 143, 891
MunI CAATTG 1 cut(s) 872
MvaI CCWGG 2 cut(s) 143, 891
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 201, 692
NcoI CCATGG 1 cut(s) 909
NdeII GATC 3 cut(s) 265, 751, 808
NlaIII CATG 2 cut(s) 758, 913
NlaIV GGNNCC 2 cut(s) 35, 722
NmuCI GTSAC 1 cut(s) 863
PceI AGGCCT 1 cut(s) 152
PciSI GCTCTTC 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PfeI GAWTC 2 cut(s) 248, 671
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 1 cut(s) 685
Ple19I CGATCG 1 cut(s) 811
PleI GAGTC 1 cut(s) 808
PpsI GAGTC 1 cut(s) 808
Ppu21I YACGTR 1 cut(s) 863
Psp6I CCWGG 2 cut(s) 141, 889
PspGI CCWGG 2 cut(s) 141, 889
PspN4I GGNNCC 2 cut(s) 35, 722
PspPI GGNCC 1 cut(s) 721
PstNI CAGNNNCTG 1 cut(s) 829
PvuI CGATCG 1 cut(s) 811
SapI GCTCTTC 1 cut(s) 478
SaqAI TTAA 5 cut(s) 333, 399, 540, 762, 997
SatI GCNGC 1 cut(s) 684
Sau3AI GATC 3 cut(s) 265, 751, 808
Sau96I GGNCC 1 cut(s) 721
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 2 cut(s) 143, 891
Sse9I AATT 4 cut(s) 574, 789, 833, 872
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 3 cut(s) 37, 252, 983
SspMI CTAG 2 cut(s) 120, 717
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 141, 889
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 3 cut(s) 466, 618, 665
TaiI ACGT 1 cut(s) 865
TaqI TCGA 4 cut(s) 261, 674, 807, 811
TasI AATT 4 cut(s) 574, 789, 833, 872
TfiI GAWTC 2 cut(s) 248, 671
Tru1I TTAA 5 cut(s) 333, 399, 540, 762, 997
Tru9I TTAA 5 cut(s) 333, 399, 540, 762, 997
TscAI CASTG 1 cut(s) 739
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 1 cut(s) 683
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 4 cut(s) 17, 456, 542, 761
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 739
XagI CCTNNNNNAGG 1 cut(s) 146
XapI RAATTY 2 cut(s) 789, 833
XcmI CCANNNNNNNNNTGG 1 cut(s) 304
XmiI GTMKAC 2 cut(s) 110, 381
XspI CTAG 2 cut(s) 120, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.