RchiOBHm_Chr4g0417771

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0021394
Physical Location & Seq
Reverse (-)
43068955 .. 43074055
5101 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38783

Sequence Viewer

Length: 1542 bp
ATGGAGGAGTTGGGTGGGTTGAAGCATTTGAGAGGCCACTTAATTATCTATAGGCTAGAACGTGCAAGGGATGGAGAAGAAGCAAAGAGTGCTTGCTTAATGGAGAAGAGTCAGTTGAGCGAGTTAACATGTACATGGACATACGATGAGCCACCTAATGACAATGAGACGGATGTACTAGATGGCTTGAAACCTCATCTTAATTTGCAAAGCTTGAACATTGAGTATTTCAAGGGTGCTAGACTTCCGTCATGGATAACGGGTCTCCAAAATTTGAAGAAGATTGGATTAGACAACTGCAACAATTGTGAAGGAATCCCAACACTCGGCCATCTACCTAATTTAAGATCTCTTTATATTGAGATGAATAAGCTGATACGTCTGGGAGCTGAGTTTTATGGTACTGGTACAACTTCATTTCGAGCACTGAAAACATTATACATCAACAAGTGCCAAGAGCTCATTGAATGGATATCAGCTGAAGGAGAAGTTGTGGTGGTATTTCCTCGCCTCGATGAGTTGCATCTCAGTGACTGTCCTAAATTGAGAAATGCTCCGAGTCGTTTTCCACGTCTCAAGAGGTTGGTGATGTATGGATTGGGAAACAGCATGCCAGTAGAAAATATAAGCGCTCAACTTACCACTCTCACTAATCTCAAAATAAGTGGTGTAAAGGGACTCACTTGTCTACCAGAAGCGATACTGAACAGTAACAATAGTCTCACACATTTAGAGGTTAGTGATTGTGATGAGTTGACGTGTATTGCTCTTGATGTATCTGACACGCTCCCTCTTCTTGAGGAGTTGAGGATAGAAGATTGTCTCAGCCTAGAGTCGATTCGGATCACACAGGGCATCGCATCTCTCCGTCAATTGAACATCGATGGTTGTGAGGCATTATCAAGCCTAGAGGTTGGGCTCGATTACTGCACCTCTCTCCAGGAGTTGGATATACATCATTGTCCTAATCTGATGTCAATTCCAACCGCACAGGGCATGCCATCTCTCCAGATATTGAAGATCTGGGCTTGTGAGGGATTATCAAGCATAGGAAGTGGGCTCAACTACTGCACCTCTCTTCAGGAATTGGAGATTGGGCATTGTCATAATCTAACATCAATTCCAATCACACAGGGCATCCCATCTCTCCGGAGCTGGAAGGTTAGTCATTGTCAGAGATTATTGTGCCTACCGAGTGTGCTCCAATTTTGCACCTCTCTTCAGTATCTGAATATTGGGCCGTTGTGGGAGGAACTGGACGCCTTCCCTGATTTTCAGCTTGCACCACCACATGATTCACAAAATTTGATCGGACTAGAAGCTCTTCCTGAGTGGTTGGGAAATCTTACATCTCTTGAGTCTCTGTGGCTCTGGGAGTGTGAGAATCTCAAGTATCTACCTACACCAAATGCTATGAAAAACCTCACCAAATTGAAGTCTCTCGTTGGTATAAGCTGTCCCCTGCTCAAAGAAACATGCACCAAGGAGACTGGCTCAGAATGGTCCAAGATTTCTCACATCCCAGATGTTCGGTTGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

513

Amino Acids

57.31

Weight (kDa)

5.19

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_R13L1-DRL21 PF25019 1 - 121 4.1e-34 R13L1/DRL21 LRRs
LRR_14 PF23598 1 - 159 3.3e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 236 - 350 1.3e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021394)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0417001 RchiOBHm_Chr4g0417771
rosa_laevigata RLG00000007931
rosa_rugosa Rorug04G0145800 Rorug04G0180600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 684
AccB7I CCANNNNNTGG 1 cut(s) 946
AccI GTMKAC 1 cut(s) 688
AccIII TCCGGA 1 cut(s) 1149
AciI CCGC 1 cut(s) 987
AclWI GGATC 1 cut(s) 851
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 271, 1303
AcuI CTGAAG 3 cut(s) 501, 1064, 1205
AcyI GRCGYC 1 cut(s) 1260
AfaI GTAC 4 cut(s) 133, 177, 403, 409
AfeI AGCGCT 1 cut(s) 631
AfiI CCNNNNNNNGG 2 cut(s) 326, 946
AflIII ACRYGT 2 cut(s) 128, 758
AgsI TTSAA 9 cut(s) 22, 190, 217, 232, 277, 467, 877, 1018, 1435
AjiI CACGTC 2 cut(s) 572, 759
AjnI CCWGG 1 cut(s) 939
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AluBI AGCT 9 cut(s) 213, 373, 389, 460, 479, 1155, 1279, 1322, 1455
AluI AGCT 9 cut(s) 213, 373, 389, 460, 479, 1155, 1279, 1322, 1455
Alw21I GWGCWC 3 cut(s) 427, 462, 1203
Alw26I GTCTC 8 cut(s) 161, 269, 578, 725, 827, 1365, 1443, 1481
AlwI GGATC 1 cut(s) 851
AlwNI CAGNNNCTG 2 cut(s) 534, 1228
Aor13HI TCCGGA 1 cut(s) 1149
Aor51HI AGCGCT 1 cut(s) 631
AoxI GGCC 3 cut(s) 34, 328, 1238
ApoI RAATTY 2 cut(s) 271, 1303
Asp700I GAANNNNTTC 1 cut(s) 1323
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 2 cut(s) 1238, 1503
AsuHPI GGTGA 2 cut(s) 598, 1417
AvaII GGWCC 1 cut(s) 1503
BanII GRGCYC 3 cut(s) 462, 921, 1062
Bbv12I GWGCWC 3 cut(s) 427, 462, 1203
BccI CCATC 6 cut(s) 65, 176, 339, 878, 1009, 1150
BceAI ACGGC 1 cut(s) 1225
BciT130I CCWGG 1 cut(s) 941
BcoDI GTCTC 8 cut(s) 161, 269, 578, 725, 827, 1365, 1443, 1481
BfaI CTAG 6 cut(s) 56, 179, 240, 830, 908, 1316
BfmI CTRYAG 1 cut(s) 49
BfoI RGCGCY 1 cut(s) 633
BglII AGATCT 2 cut(s) 347, 1020
Bme1390I CCNGG 1 cut(s) 941
Bme18I GGWCC 1 cut(s) 1503
BmgBI CACGTC 2 cut(s) 572, 759
BmgT120I GGNCC 2 cut(s) 1238, 1503
BmrFI CCNGG 1 cut(s) 941
BmsI GCATC 4 cut(s) 532, 864, 869, 1146
BoxI GACNNNNGTC 1 cut(s) 247
BplI GAGNNNNNCTC 4 cut(s) 538, 570, 1478, 1510
BpmI CTGGAG 2 cut(s) 923, 992
BpuEI CTTGAG 4 cut(s) 560, 818, 1373, 1376
Bsa29I ATCGAT 1 cut(s) 882
BsaBI GATNNNNATC 2 cut(s) 842, 954
BsaHI GRCGYC 1 cut(s) 1260
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 1482
BsaWI WCCGGW 1 cut(s) 1149
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 946
Bse1I ACTGG 4 cut(s) 409, 614, 1260, 1495
Bse8I GATNNNNATC 2 cut(s) 842, 954
BseAI TCCGGA 1 cut(s) 1149
BseBI CCWGG 1 cut(s) 941
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 1 cut(s) 1482
BseGI GGATG 4 cut(s) 76, 178, 1137, 1518
BseJI GATNNNNATC 2 cut(s) 842, 954
BseLI CCNNNNNNNGG 2 cut(s) 326, 946
BseMII CTCAG 5 cut(s) 381, 541, 838, 1320, 1509
BseNI ACTGG 4 cut(s) 409, 614, 1260, 1495
BseRI GAGGAG 2 cut(s) 20, 815
BsgI GTGCAG 2 cut(s) 913, 1054
BshFI GGCC 3 cut(s) 36, 330, 1240
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 3 cut(s) 427, 462, 1203
BsiSI CCGG 1 cut(s) 1150
BslFI GGGAC 2 cut(s) 690, 1443
BslI CCNNNNNNNGG 2 cut(s) 326, 946
BsmAI GTCTC 8 cut(s) 161, 269, 578, 725, 827, 1365, 1443, 1481
BsmBI CGTCTC 2 cut(s) 161, 578
BsmFI GGGAC 2 cut(s) 690, 1443
BsnI GGCC 3 cut(s) 36, 330, 1240
Bso31I GGTCTC 1 cut(s) 269
Bsp1286I GDGCHC 5 cut(s) 427, 462, 921, 1062, 1203
Bsp13I TCCGGA 1 cut(s) 1149
Bsp1407I TGTACA 1 cut(s) 131
Bsp143I GATC 4 cut(s) 347, 843, 1020, 1308
BspACI CCGC 1 cut(s) 987
BspANI GGCC 3 cut(s) 36, 330, 1240
BspCNI CTCAG 5 cut(s) 382, 540, 837, 1321, 1508
BspDI ATCGAT 1 cut(s) 882
BspEI TCCGGA 1 cut(s) 1149
BspPI GGATC 1 cut(s) 851
BspQI GCTCTTC 1 cut(s) 1329
BspTNI GGTCTC 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 131
BsrI ACTGG 4 cut(s) 409, 614, 1260, 1495
BssECI CCNNGG 1 cut(s) 1482
BssMI GATC 4 cut(s) 347, 843, 1020, 1308
BssNI GRCGYC 1 cut(s) 1260
BssT1I CCWWGG 1 cut(s) 1482
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 2 cut(s) 536, 710
Bst6I CTCTTC 5 cut(s) 101, 798, 1083, 1224, 1329
BstACI GRCGYC 1 cut(s) 1260
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 131
BstC8I GCNNGC 4 cut(s) 94, 611, 998, 1281
BstDEI CTNAG 5 cut(s) 390, 527, 824, 1329, 1495
BstF5I GGATG 4 cut(s) 76, 178, 1137, 1518
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 4 cut(s) 350, 846, 1023, 1311
BstMAI GTCTC 8 cut(s) 161, 269, 578, 725, 827, 1365, 1443, 1481
BstMBI GATC 4 cut(s) 347, 843, 1020, 1308
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 1 cut(s) 941
BstNSI RCATGY 4 cut(s) 132, 613, 1000, 1479
BstPAI GACNNNNGTC 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 939
BstSFI CTRYAG 1 cut(s) 49
BstX2I RGATCY 2 cut(s) 347, 1020
BstYI RGATCY 2 cut(s) 347, 1020
Bsu15I ATCGAT 1 cut(s) 882
BsuRI GGCC 3 cut(s) 36, 330, 1240
BsuTUI ATCGAT 1 cut(s) 882
BtgZI GCGATG 1 cut(s) 841
BtrI CACGTC 2 cut(s) 572, 759
BtsCI GGATG 4 cut(s) 76, 178, 1137, 1518
BtsIMutI CAGTG 2 cut(s) 425, 535
Cac8I GCNNGC 4 cut(s) 94, 611, 998, 1281
CaiI CAGNNNCTG 2 cut(s) 534, 1228
CfoI GCGC 1 cut(s) 632
Cfr13I GGNCC 2 cut(s) 1238, 1503
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 1 cut(s) 1268
Csp6I GTAC 4 cut(s) 132, 176, 402, 408
CspCI CAANNNNNGTGG 2 cut(s) 646, 681
CviAII CATG 7 cut(s) 129, 135, 252, 610, 997, 1292, 1476
CviQI GTAC 4 cut(s) 132, 176, 402, 408
DdeI CTNAG 5 cut(s) 390, 527, 824, 1329, 1495
DpnI GATC 4 cut(s) 349, 845, 1022, 1310
DpnII GATC 4 cut(s) 347, 843, 1020, 1308
DrdI GACNNNNNNGTC 1 cut(s) 684
DseDI GACNNNNNNGTC 1 cut(s) 684
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 5 cut(s) 101, 798, 1083, 1224, 1329
EarI CTCTTC 5 cut(s) 101, 798, 1083, 1224, 1329
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 1482
Eco24I GRGCYC 3 cut(s) 462, 921, 1062
Eco31I GGTCTC 1 cut(s) 269
Eco32I GATATC 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 1503
Eco47III AGCGCT 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 460
Eco57I CTGAAG 3 cut(s) 501, 1064, 1205
EcoICRI GAGCTC 1 cut(s) 460
EcoRII CCWGG 1 cut(s) 939
EcoRV GATATC 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 1482
EcoT38I GRGCYC 3 cut(s) 462, 921, 1062
ErhI CCWWGG 1 cut(s) 1482
Esp3I CGTCTC 2 cut(s) 161, 578
FaeI CATG 7 cut(s) 132, 138, 255, 613, 1000, 1295, 1479
FaqI GGGAC 2 cut(s) 690, 1443
FatI CATG 7 cut(s) 128, 134, 251, 609, 996, 1291, 1475
FblI GTMKAC 1 cut(s) 688
FokI GGATG 4 cut(s) 83, 185, 1124, 1505
FriOI GRGCYC 3 cut(s) 462, 921, 1062
FspBI CTAG 6 cut(s) 56, 179, 240, 830, 908, 1316
GlaI GCGC 1 cut(s) 631
GsuI CTGGAG 2 cut(s) 923, 992
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 3 cut(s) 36, 330, 1240
HapII CCGG 1 cut(s) 1150
HgaI GACGC 1 cut(s) 1268
HhaI GCGC 1 cut(s) 632
Hin1I GRCGYC 1 cut(s) 1260
Hin1II CATG 7 cut(s) 132, 138, 255, 613, 1000, 1295, 1479
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 2 cut(s) 126, 756
HindII GTYRAC 2 cut(s) 126, 756
HindIII AAGCTT 1 cut(s) 211
HinfI GANTC 9 cut(s) 109, 315, 559, 678, 833, 838, 1295, 1358, 1384
HpaI GTTAAC 1 cut(s) 126
HpaII CCGG 1 cut(s) 1150
HphI GGTGA 2 cut(s) 598, 1417
Hpy166II GTNNAC 3 cut(s) 126, 689, 756
Hpy188I TCNGA 8 cut(s) 558, 781, 843, 972, 1176, 1230, 1313, 1498
Hpy188III TCNNGA 8 cut(s) 577, 770, 797, 1009, 1082, 1150, 1328, 1355
Hpy8I GTNNAC 3 cut(s) 126, 689, 756
HpyAV CCTTC 4 cut(s) 305, 476, 1153, 1273
HpyCH4III ACNGT 2 cut(s) 536, 710
HpyCH4IV ACGT 4 cut(s) 61, 379, 571, 758
HpyCH4V TGCA 9 cut(s) 65, 208, 300, 523, 930, 1071, 1212, 1283, 1479
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 5 cut(s) 390, 527, 824, 1329, 1495
HpySE526I ACGT 4 cut(s) 61, 379, 571, 758
Hsp92I GRCGYC 1 cut(s) 1260
Hsp92II CATG 7 cut(s) 132, 138, 255, 613, 1000, 1295, 1479
HspAI GCGC 1 cut(s) 630
Kpn2I TCCGGA 1 cut(s) 1149
KspAI GTTAAC 1 cut(s) 126
Kzo9I GATC 4 cut(s) 347, 843, 1020, 1308
LguI GCTCTTC 1 cut(s) 1329
LmnI GCTCC 5 cut(s) 386, 559, 792, 1152, 1206
LweI GCATC 4 cut(s) 532, 864, 869, 1146
MaeI CTAG 6 cut(s) 56, 179, 240, 830, 908, 1316
MaeII ACGT 4 cut(s) 61, 379, 571, 758
MaeIII GTNAC 2 cut(s) 530, 710
MalI GATC 4 cut(s) 349, 845, 1022, 1310
MboI GATC 4 cut(s) 347, 843, 1020, 1308
MfeI CAATTG 2 cut(s) 304, 872
MflI RGATCY 2 cut(s) 347, 1020
MhlI GDGCHC 5 cut(s) 427, 462, 921, 1062, 1203
MlyI GAGTC 5 cut(s) 118, 568, 672, 842, 1367
MmeI TCCRAC 2 cut(s) 927, 1007
MroI TCCGGA 1 cut(s) 1149
MroXI GAANNNNTTC 1 cut(s) 1323
MseI TTAA 5 cut(s) 41, 98, 125, 201, 344
MslI CAYNNNNRTG 2 cut(s) 133, 528
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 1 cut(s) 1150
MspR9I CCNGG 1 cut(s) 941
MunI CAATTG 2 cut(s) 304, 872
MvaI CCWGG 1 cut(s) 941
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeII GATC 4 cut(s) 347, 843, 1020, 1308
NlaIII CATG 7 cut(s) 132, 138, 255, 613, 1000, 1295, 1479
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 1 cut(s) 530
NspI RCATGY 4 cut(s) 132, 613, 1000, 1479
PaeI GCATGC 2 cut(s) 613, 1000
PciI ACATGT 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 1329
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 1323
PfeI GAWTC 4 cut(s) 315, 838, 1295, 1384
PflMI CCANNNNNTGG 1 cut(s) 946
PfoI TCCNGGA 1 cut(s) 939
PleI GAGTC 5 cut(s) 117, 567, 672, 841, 1366
PpsI GAGTC 5 cut(s) 117, 567, 672, 841, 1366
PscI ACATGT 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 462
Psp6I CCWGG 1 cut(s) 939
PspGI CCWGG 1 cut(s) 939
PspPI GGNCC 2 cut(s) 1238, 1503
PstNI CAGNNNCTG 2 cut(s) 534, 1228
PsuI RGATCY 2 cut(s) 347, 1020
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 4 cut(s) 133, 177, 403, 409
RsaNI GTAC 4 cut(s) 132, 176, 402, 408
RseI CAYNNNNRTG 2 cut(s) 133, 528
SacI GAGCTC 1 cut(s) 462
SapI GCTCTTC 1 cut(s) 1329
SaqAI TTAA 5 cut(s) 41, 98, 125, 201, 344
Sau3AI GATC 4 cut(s) 347, 843, 1020, 1308
Sau96I GGNCC 2 cut(s) 1238, 1503
SchI GAGTC 5 cut(s) 118, 568, 672, 842, 1367
ScrFI CCNGG 1 cut(s) 941
SduI GDGCHC 5 cut(s) 427, 462, 921, 1062, 1203
SfaNI GCATC 4 cut(s) 532, 864, 869, 1146
SfcI CTRYAG 1 cut(s) 49
SinI GGWCC 1 cut(s) 1503
SmiMI CAYNNNNRTG 2 cut(s) 133, 528
SmlI CTYRAG 4 cut(s) 575, 797, 1355, 1388
SmoI CTYRAG 4 cut(s) 575, 797, 1355, 1388
SphI GCATGC 2 cut(s) 613, 1000
SsiI CCGC 1 cut(s) 987
SspI AATATT 1 cut(s) 1234
SspMI CTAG 6 cut(s) 56, 179, 240, 830, 908, 1316
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 939
StyI CCWWGG 1 cut(s) 1482
TaaI ACNGT 2 cut(s) 536, 710
TaiI ACGT 4 cut(s) 64, 382, 574, 761
TaqI TCGA 5 cut(s) 421, 513, 836, 882, 921
TatI WGTACW 2 cut(s) 131, 175
TfiI GAWTC 4 cut(s) 315, 838, 1295, 1384
Tru1I TTAA 5 cut(s) 41, 98, 125, 201, 344
Tru9I TTAA 5 cut(s) 41, 98, 125, 201, 344
TscAI CASTG 2 cut(s) 432, 535
TseFI GTSAC 1 cut(s) 530
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 3 cut(s) 380, 405, 1430
TspGWI ACGGA 3 cut(s) 185, 237, 857
TspRI CASTG 2 cut(s) 432, 535
Van91I CCANNNNNTGG 1 cut(s) 946
VpaK11BI GGWCC 1 cut(s) 1503
XapI RAATTY 2 cut(s) 271, 1303
XceI RCATGY 4 cut(s) 132, 613, 1000, 1479
XmiI GTMKAC 1 cut(s) 688
XmnI GAANNNNTTC 1 cut(s) 1323
XspI CTAG 6 cut(s) 56, 179, 240, 830, 908, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.