RchiOBHm_Chr5g0037381

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0022780
Physical Location & Seq
Reverse (-)
31799206 .. 31800578
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31609

Sequence Viewer

Length: 906 bp
ATGTTTCTCATTGTTACCCTATTTTTCTTGCCATTGTTTGCTGAAGCCACACCACGCATGCCCCCAACAAAACCGAGTTCCATTAATACCACCACCACCTTATCATCAAACTCATCTTTTAATTTTAGGTACAAAAGCTTGAACCTTAACGATCTTCCTACAGAAATCGATTGGGTAAAAGAAAGACTAGTTACCCCAGTAAAGGACCAACTAGGGTGTGGTAGTTGCTGGGCGTTTGCAGCTGTAGCTGCTGTCGAAGGAATTGCCCAAAGAGCGACTGGCGAATTGAGAGTATTGTCGGAGCAACAACTATTGGACTGCAGTAGAGACTTTGGTACCAATGGCTGCAGGGGAAGTGGTCACAGATTGAAGGCCTTTAATTACATTAAAGAACATGGAGTCGCTTATAGTGACAAGTATCCATACAAAGGTACGGACACGCAAGCATGTGACAACACCACCGTCAGTTATCCAGCAGCCACAATAACTGGCTATGAACGGGTCCCTAATAATGAAATGGATCTGATAAGAGCTGTGGCCAATCAACCAGTCACTGCCTCAGTTTTCACATGTGACGAATTTAATTATTACACAGGTGGTTATATATTTGAGGACCGAGAGGGGAAATGTAATTTTGATATAGATAAGGCGAACCATGCCATTACTATAGTTGGGTACGGGACTGAAAACGGTGTAGATTATTGGAAAATAAAAAATTCATGGGGTGAGGATTGGGGTGACAAGGGTTATATGAAAATGCGAAGGAATGTTGCGACTAAGCCGGAAGGTATTTGCGGAATTGCTAGTGCTCCTATATACCCAACCGATGATATTGCTCCGTCCCGGTCAAACCCCGAAGGTCCAAGTAAGATAGTTGGGGGTCCAGGTCAAACCCCGGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

301

Amino Acids

33.12

Weight (kDa)

5.38

Isoelectric Point (pI)

24.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 52 - 275 1.5e-66 Papain family cysteine protease
Peptidase_C1_2 PF03051 209 - 255 1.5e-06 Peptidase C1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022780)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0037381
rosa_laevigata RLG00000033778
rosa_rugosa Rorug05G0164900
rosa_samantha Rh5AG254000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AciI CCGC 1 cut(s) 795
AclWI GGATC 1 cut(s) 528
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 578, 715
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 4 cut(s) 131, 337, 433, 677
AfiI CCNNNNNNNGG 2 cut(s) 202, 428
AflIII ACRYGT 1 cut(s) 569
AgsI TTSAA 2 cut(s) 142, 370
AhlI ACTAGT 1 cut(s) 187
AjnI CCWGG 1 cut(s) 883
AluBI AGCT 4 cut(s) 138, 242, 248, 533
AluI AGCT 4 cut(s) 138, 242, 248, 533
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 528
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 2 cut(s) 372, 537
ApeKI GCWGC 4 cut(s) 239, 248, 345, 476
ApoI RAATTY 2 cut(s) 578, 715
AseI ATTAAT 1 cut(s) 84
Asp718I GGTACC 1 cut(s) 335
AspS9I GGNCC 5 cut(s) 205, 502, 613, 860, 881
AsuC2I CCSGG 2 cut(s) 844, 896
AsuHPI GGTGA 2 cut(s) 737, 749
AvaII GGWCC 5 cut(s) 205, 502, 613, 860, 881
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BalI TGGCCA 1 cut(s) 539
BanI GGYRCC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 4 cut(s) 235, 251, 332, 488
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 815, 849
BciT130I CCWGG 1 cut(s) 885
BciVI GTATCC 1 cut(s) 429
BcnI CCSGG 2 cut(s) 844, 896
BcoDI GTCTC 1 cut(s) 321
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 3 cut(s) 188, 212, 804
BfmI CTRYAG 5 cut(s) 159, 243, 319, 346, 666
BfuI GTATCC 1 cut(s) 429
BisI GCNGC 4 cut(s) 240, 249, 346, 477
BlsI GCNGC 4 cut(s) 241, 250, 347, 478
Bme1390I CCNGG 3 cut(s) 844, 885, 896
Bme18I GGWCC 5 cut(s) 205, 502, 613, 860, 881
BmgT120I GGNCC 5 cut(s) 205, 502, 613, 860, 881
BmiI GGNNCC 4 cut(s) 337, 503, 504, 882
BmrFI CCNGG 3 cut(s) 844, 885, 896
BmrI ACTGGG 1 cut(s) 191
BmuI ACTGGG 1 cut(s) 191
BpuMI CCSGG 2 cut(s) 844, 896
Bsa29I ATCGAT 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 894
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 428
Bse1I ACTGG 4 cut(s) 197, 283, 493, 548
BseBI CCWGG 1 cut(s) 885
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 894
BseLI CCNNNNNNNGG 2 cut(s) 202, 428
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 4 cut(s) 197, 283, 493, 548
BseXI GCAGC 4 cut(s) 235, 251, 332, 488
BseYI CCCAGC 1 cut(s) 228
BshFI GGCC 2 cut(s) 374, 539
BshNI GGYRCC 1 cut(s) 335
BshVI ATCGAT 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 3 cut(s) 782, 844, 896
BslFI GGGAC 3 cut(s) 488, 694, 826
BslI CCNNNNNNNGG 2 cut(s) 202, 428
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 3 cut(s) 488, 694, 826
BsnI GGCC 2 cut(s) 374, 539
Bsp1286I GDGCHC 1 cut(s) 811
Bsp143I GATC 2 cut(s) 151, 520
BspACI CCGC 1 cut(s) 795
BspANI GGCC 2 cut(s) 374, 539
BspCNI CTCAG 1 cut(s) 572
BspDI ATCGAT 1 cut(s) 168
BspLI GGNNCC 4 cut(s) 337, 503, 504, 882
BspMAI CTGCAG 2 cut(s) 323, 350
BspPI GGATC 1 cut(s) 528
BspT107I GGYRCC 1 cut(s) 335
BsrI ACTGG 4 cut(s) 197, 283, 493, 548
BssECI CCNNGG 1 cut(s) 894
BssMI GATC 2 cut(s) 151, 520
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 2 cut(s) 463, 692
BstC8I GCNNGC 2 cut(s) 59, 444
BstDEI CTNAG 2 cut(s) 559, 777
BstKTI GATC 2 cut(s) 154, 523
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 2 cut(s) 151, 520
BstMWI GCNNNNNNNGC 5 cut(s) 239, 245, 248, 272, 656
BstNI CCWGG 1 cut(s) 885
BstNSI RCATGY 3 cut(s) 61, 450, 573
BstSCI CCNGG 3 cut(s) 842, 883, 894
BstSFI CTRYAG 5 cut(s) 159, 243, 319, 346, 666
BstV1I GCAGC 4 cut(s) 235, 251, 332, 488
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 168
BsuI GTATCC 1 cut(s) 429
BsuRI GGCC 2 cut(s) 374, 539
BsuTUI ATCGAT 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 2 cut(s) 59, 444
CaiI CAGNNNCTG 1 cut(s) 554
Cfr13I GGNCC 5 cut(s) 205, 502, 613, 860, 881
ClaI ATCGAT 1 cut(s) 168
Csp6I GTAC 4 cut(s) 130, 336, 432, 676
CviAII CATG 6 cut(s) 58, 395, 447, 570, 656, 720
CviQI GTAC 4 cut(s) 130, 336, 432, 676
DdeI CTNAG 2 cut(s) 559, 777
DpnI GATC 2 cut(s) 153, 522
DpnII GATC 2 cut(s) 151, 520
EaeI YGGCCR 1 cut(s) 537
Eco147I AGGCCT 1 cut(s) 374
Eco47I GGWCC 5 cut(s) 205, 502, 613, 860, 881
Eco57I CTGAAG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 1 cut(s) 883
FaeI CATG 6 cut(s) 61, 398, 450, 573, 659, 723
FaqI GGGAC 3 cut(s) 488, 694, 826
FatI CATG 6 cut(s) 57, 394, 446, 569, 655, 719
Fnu4HI GCNGC 4 cut(s) 240, 249, 346, 477
Fsp4HI GCNGC 4 cut(s) 240, 249, 346, 477
FspBI CTAG 3 cut(s) 188, 212, 804
GluI GCNGC 4 cut(s) 240, 249, 346, 477
GsaI CCCAGC 1 cut(s) 232
HaeIII GGCC 2 cut(s) 374, 539
HapII CCGG 3 cut(s) 782, 844, 896
Hin1II CATG 6 cut(s) 61, 398, 450, 573, 659, 723
HindIII AAGCTT 1 cut(s) 136
HinfI GANTC 1 cut(s) 399
HpaII CCGG 3 cut(s) 782, 844, 896
HphI GGTGA 2 cut(s) 737, 749
Hpy188I TCNGA 2 cut(s) 301, 525
HpyAV CCTTC 6 cut(s) 251, 364, 756, 779, 851, 893
HpyCH4III ACNGT 2 cut(s) 463, 692
HpyCH4V TGCA 3 cut(s) 239, 321, 348
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 245, 248, 272, 656
HpyF3I CTNAG 2 cut(s) 559, 777
Hsp92II CATG 6 cut(s) 61, 398, 450, 573, 659, 723
KflI GGGWCCC 1 cut(s) 502
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 2 cut(s) 151, 520
LmnI GCTCC 3 cut(s) 301, 814, 841
Lsp1109I GCAGC 4 cut(s) 235, 251, 332, 488
MaeI CTAG 3 cut(s) 188, 212, 804
MaeIII GTNAC 8 cut(s) 13, 190, 359, 410, 449, 550, 572, 737
MalI GATC 2 cut(s) 153, 522
MboI GATC 2 cut(s) 151, 520
MboII GAAGA 1 cut(s) 146
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 811
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 9 cut(s) 121, 261, 284, 379, 578, 583, 631, 715, 798
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 1 cut(s) 408
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 4 cut(s) 568, 604, 613, 721
Mox20I TGGCCA 1 cut(s) 539
MscI TGGCCA 1 cut(s) 539
MseI TTAA 6 cut(s) 84, 120, 147, 378, 387, 582
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 3 cut(s) 782, 844, 896
MspR9I CCNGG 3 cut(s) 844, 885, 896
MvaI CCWGG 1 cut(s) 885
MwoI GCNNNNNNNGC 5 cut(s) 239, 245, 248, 272, 656
NciI CCSGG 2 cut(s) 844, 896
NdeII GATC 2 cut(s) 151, 520
NlaIII CATG 6 cut(s) 61, 398, 450, 573, 659, 723
NlaIV GGNNCC 4 cut(s) 337, 503, 504, 882
NmuCI GTSAC 6 cut(s) 359, 410, 449, 550, 572, 737
NspI RCATGY 3 cut(s) 61, 450, 573
PaeI GCATGC 1 cut(s) 61
PceI AGGCCT 1 cut(s) 374
PciI ACATGT 1 cut(s) 569
PkrI GCNGC 4 cut(s) 241, 250, 347, 478
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PpuMI RGGWCCY 1 cut(s) 502
PscI ACATGT 1 cut(s) 569
PshBI ATTAAT 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 228
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 4 cut(s) 337, 503, 504, 882
PspPI GGNCC 5 cut(s) 205, 502, 613, 860, 881
PspPPI RGGWCCY 1 cut(s) 502
PstI CTGCAG 2 cut(s) 323, 350
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 520
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 4 cut(s) 131, 337, 433, 677
RsaNI GTAC 4 cut(s) 130, 336, 432, 676
SaqAI TTAA 6 cut(s) 84, 120, 147, 378, 387, 582
SatI GCNGC 4 cut(s) 240, 249, 346, 477
Sau3AI GATC 2 cut(s) 151, 520
Sau96I GGNCC 5 cut(s) 205, 502, 613, 860, 881
SchI GAGTC 1 cut(s) 408
ScrFI CCNGG 3 cut(s) 844, 885, 896
SduI GDGCHC 1 cut(s) 811
SfcI CTRYAG 5 cut(s) 159, 243, 319, 346, 666
SinI GGWCC 5 cut(s) 205, 502, 613, 860, 881
SpeI ACTAGT 1 cut(s) 187
SphI GCATGC 1 cut(s) 61
Sse9I AATT 9 cut(s) 121, 261, 284, 379, 578, 583, 631, 715, 798
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 1 cut(s) 795
SspMI CTAG 3 cut(s) 188, 212, 804
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 3 cut(s) 842, 883, 894
TaaI ACNGT 2 cut(s) 463, 692
TaqI TCGA 2 cut(s) 168, 255
TaqII GACCGA 1 cut(s) 630
TasI AATT 9 cut(s) 121, 261, 284, 379, 578, 583, 631, 715, 798
Tru1I TTAA 6 cut(s) 84, 120, 147, 378, 387, 582
Tru9I TTAA 6 cut(s) 84, 120, 147, 378, 387, 582
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 6 cut(s) 359, 410, 449, 550, 572, 737
TseI GCWGC 4 cut(s) 239, 248, 345, 476
Tsp45I GTSAC 6 cut(s) 359, 410, 449, 550, 572, 737
TspDTI ATGAA 4 cut(s) 510, 528, 708, 767
TspGWI ACGGA 2 cut(s) 449, 828
TspRI CASTG 1 cut(s) 559
VpaK11BI GGWCC 5 cut(s) 205, 502, 613, 860, 881
VspI ATTAAT 1 cut(s) 84
XapI RAATTY 2 cut(s) 578, 715
XceI RCATGY 3 cut(s) 61, 450, 573
XcmI CCANNNNNNNNNTGG 2 cut(s) 215, 275
XspI CTAG 3 cut(s) 188, 212, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.