RLG00000033778

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0022780
Physical Location & Seq
Reverse (-)
32028491 .. 32029870
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033778

Sequence Viewer

Length: 927 bp
ATGGATCAGAAAATGTTTCTCATTGTTACCCTATTTTTCTTGTCATTGTTTGCTGAAGCCACTTCACGCATGCCCCCAACAAAACCGAGTTCCATTAATACCACCACCACCTTATCATCAAACTCATCTTTTAATTTTAGGTACAAAAGCTTGAACCTTAACGATCTTCCCACAGAAATCGACTGGGTAAAAGAAGGACTAGTTACCCCTGTAAAGGACCAACTAGAGTGTGGTAGTTGCTGGGCGTTTGCAGCAGTAGCTGCTGTCGAAGGAATTGCCCAAAGAGCGACTGGCGAATTGAGAGTATTGTCGGAGCAACAACTATTGGACTGCAGTAGAGACTTTGGTACCAATGGCTGCAGCGGAAGTGGTCACAGATTGAAGGCCTTTAATTACATTAAAGAACATGGAGTCGCTTATAGTGACAAATATCCATACAAAGGTACGGACCAAGCATGTGACAACACCACCGTCAGTTATCCAGCAGCCACAATAACTGGCTATGAACGGGTCCCTAATAATGAAATGGATCTGATGAGAGCTGTTGCCAATCAACCAGTCACTGCCTCAGTTTTCACATGTGACGAATTTAATTATTACGCAGGTGGTTATATATTTGAGGACCGAGAGGGGAAATGTAATTTTGATATAGATAAGGAGAACCATGCCATTACTATAGTTGGGTACGGGACTGAAAACGGTGTAGATTATTGGAAAATAAAAAATTCATGGGGTGAGGAATGGGGTGATAAGGGTTACATGAAAATGCGTAGGAATGTTGCGACCAGGCCGGAAGGTATTTGCGGAATTGCTAGTGCTCCTATATACCCAACCGATGATATTGCTCCGTCCCGGTCAAACCCCGAAGGTCCAAGTAAAATAGTTGGGGGTCCAGGTCAAATCCCGGAAGGACAGACATACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

309

Amino Acids

34.01

Weight (kDa)

5.0

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 56 - 278 5.1e-67 Papain family cysteine protease
Peptidase_C1_2 PF03051 214 - 258 7.5e-06 Peptidase C1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022780)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0037381
rosa_laevigata RLG00000033778
rosa_rugosa Rorug05G0164900
rosa_samantha Rh5AG254000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 593
Acc36I ACCTGC 1 cut(s) 593
Acc65I GGTACC 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 347
AciI CCGC 2 cut(s) 363, 804
AclWI GGATC 2 cut(s) 12, 537
AcsI RAATTY 2 cut(s) 587, 724
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 4 cut(s) 143, 349, 445, 686
AfiI CCNNNNNNNGG 2 cut(s) 214, 440
AflIII ACRYGT 1 cut(s) 578
AgsI TTSAA 2 cut(s) 154, 382
AhlI ACTAGT 1 cut(s) 199
AjnI CCWGG 2 cut(s) 785, 892
AluBI AGCT 3 cut(s) 150, 260, 542
AluI AGCT 3 cut(s) 150, 260, 542
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 1 cut(s) 333
AlwI GGATC 2 cut(s) 12, 537
AlwNI CAGNNNCTG 2 cut(s) 260, 563
AoxI GGCC 2 cut(s) 384, 788
ApeKI GCWGC 5 cut(s) 251, 260, 357, 360, 485
ApoI RAATTY 2 cut(s) 587, 724
AseI ATTAAT 1 cut(s) 96
Asp718I GGTACC 1 cut(s) 347
AspS9I GGNCC 6 cut(s) 217, 448, 511, 622, 869, 890
AsuC2I CCSGG 2 cut(s) 853, 905
AsuHPI GGTGA 2 cut(s) 746, 758
AvaII GGWCC 6 cut(s) 217, 448, 511, 622, 869, 890
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BanI GGYRCC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 820
BbvI GCAGC 5 cut(s) 247, 263, 344, 372, 497
BcgI CGANNNNNNTGC 4 cut(s) 257, 291, 824, 858
BciT130I CCWGG 2 cut(s) 787, 894
BcnI CCSGG 2 cut(s) 853, 905
BcoDI GTCTC 1 cut(s) 333
BcuI ACTAGT 1 cut(s) 199
BfaI CTAG 3 cut(s) 200, 224, 813
BfmI CTRYAG 3 cut(s) 331, 358, 675
BfuAI ACCTGC 1 cut(s) 593
BisI GCNGC 5 cut(s) 252, 261, 358, 361, 486
BlsI GCNGC 5 cut(s) 253, 262, 359, 362, 487
Bme1390I CCNGG 4 cut(s) 787, 853, 894, 905
Bme18I GGWCC 6 cut(s) 217, 448, 511, 622, 869, 890
BmgT120I GGNCC 6 cut(s) 217, 448, 511, 622, 869, 890
BmiI GGNNCC 4 cut(s) 349, 512, 513, 891
BmrFI CCNGG 4 cut(s) 787, 853, 894, 905
BmrI ACTGGG 1 cut(s) 193
BmuI ACTGGG 1 cut(s) 193
BpuMI CCSGG 2 cut(s) 853, 905
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 440
Bse1I ACTGG 4 cut(s) 188, 295, 502, 557
BseBI CCWGG 2 cut(s) 787, 894
BseLI CCNNNNNNNGG 2 cut(s) 214, 440
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 4 cut(s) 188, 295, 502, 557
BseXI GCAGC 5 cut(s) 247, 263, 344, 372, 497
BseYI CCCAGC 1 cut(s) 240
BshFI GGCC 2 cut(s) 386, 790
BshNI GGYRCC 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 820
BsiSI CCGG 3 cut(s) 791, 853, 905
BslFI GGGAC 3 cut(s) 497, 703, 835
BslI CCNNNNNNNGG 2 cut(s) 214, 440
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 3 cut(s) 497, 703, 835
BsnI GGCC 2 cut(s) 386, 790
Bsp1286I GDGCHC 1 cut(s) 820
Bsp143I GATC 3 cut(s) 4, 163, 529
BspACI CCGC 2 cut(s) 363, 804
BspANI GGCC 2 cut(s) 386, 790
BspCNI CTCAG 1 cut(s) 581
BspLI GGNNCC 4 cut(s) 349, 512, 513, 891
BspMAI CTGCAG 2 cut(s) 335, 362
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 2 cut(s) 12, 537
BspT107I GGYRCC 1 cut(s) 347
BsrI ACTGG 4 cut(s) 188, 295, 502, 557
BssMI GATC 3 cut(s) 4, 163, 529
Bst2UI CCWGG 2 cut(s) 787, 894
Bst4CI ACNGT 2 cut(s) 472, 701
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 568
BstKTI GATC 3 cut(s) 7, 166, 532
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 3 cut(s) 4, 163, 529
BstMWI GCNNNNNNNGC 4 cut(s) 251, 257, 260, 284
BstNI CCWGG 2 cut(s) 787, 894
BstNSI RCATGY 3 cut(s) 73, 459, 582
BstSCI CCNGG 4 cut(s) 785, 851, 892, 903
BstSFI CTRYAG 3 cut(s) 331, 358, 675
BstV1I GCAGC 5 cut(s) 247, 263, 344, 372, 497
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 2 cut(s) 386, 790
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 561
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 1 cut(s) 71
CaiI CAGNNNCTG 2 cut(s) 260, 563
Cfr13I GGNCC 6 cut(s) 217, 448, 511, 622, 869, 890
Csp6I GTAC 4 cut(s) 142, 348, 444, 685
CviAII CATG 7 cut(s) 70, 407, 456, 579, 665, 729, 760
CviJI RGCY 9 cut(s) 59, 150, 260, 357, 386, 488, 501, 542, 790
CviKI_1 RGCY 9 cut(s) 59, 150, 260, 357, 386, 488, 501, 542, 790
CviQI GTAC 4 cut(s) 142, 348, 444, 685
DdeI CTNAG 1 cut(s) 568
DpnI GATC 3 cut(s) 6, 165, 531
DpnII GATC 3 cut(s) 4, 163, 529
Eco147I AGGCCT 1 cut(s) 386
Eco47I GGWCC 6 cut(s) 217, 448, 511, 622, 869, 890
Eco57I CTGAAG 1 cut(s) 75
EcoO109I RGGNCCY 1 cut(s) 511
EcoRII CCWGG 2 cut(s) 785, 892
FaeI CATG 7 cut(s) 73, 410, 459, 582, 668, 732, 763
FaqI GGGAC 3 cut(s) 497, 703, 835
FatI CATG 7 cut(s) 69, 406, 455, 578, 664, 728, 759
Fnu4HI GCNGC 5 cut(s) 252, 261, 358, 361, 486
Fsp4HI GCNGC 5 cut(s) 252, 261, 358, 361, 486
FspBI CTAG 3 cut(s) 200, 224, 813
GluI GCNGC 5 cut(s) 252, 261, 358, 361, 486
GsaI CCCAGC 1 cut(s) 244
HaeIII GGCC 2 cut(s) 386, 790
HapII CCGG 3 cut(s) 791, 853, 905
Hin1II CATG 7 cut(s) 73, 410, 459, 582, 668, 732, 763
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 1 cut(s) 411
HpaII CCGG 3 cut(s) 791, 853, 905
HphI GGTGA 2 cut(s) 746, 758
Hpy188I TCNGA 3 cut(s) 9, 313, 534
HpyAV CCTTC 6 cut(s) 188, 263, 376, 788, 860, 902
HpyCH4III ACNGT 2 cut(s) 472, 701
HpyCH4V TGCA 3 cut(s) 251, 333, 360
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 257, 260, 284
HpyF3I CTNAG 1 cut(s) 568
Hsp92II CATG 7 cut(s) 73, 410, 459, 582, 668, 732, 763
KflI GGGWCCC 1 cut(s) 511
KpnI GGTACC 1 cut(s) 351
Kzo9I GATC 3 cut(s) 4, 163, 529
LmnI GCTCC 3 cut(s) 313, 823, 850
Lsp1109I GCAGC 5 cut(s) 247, 263, 344, 372, 497
MaeI CTAG 3 cut(s) 200, 224, 813
MaeIII GTNAC 8 cut(s) 25, 202, 371, 422, 458, 559, 581, 755
MalI GATC 3 cut(s) 6, 165, 531
MboI GATC 3 cut(s) 4, 163, 529
MboII GAAGA 1 cut(s) 158
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 1 cut(s) 820
MlyI GAGTC 1 cut(s) 420
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 4 cut(s) 577, 613, 622, 730
MseI TTAA 7 cut(s) 96, 132, 159, 390, 399, 591, 925
MslI CAYNNNNRTG 1 cut(s) 764
MspA1I CMGCKG 1 cut(s) 363
MspI CCGG 3 cut(s) 791, 853, 905
MspR9I CCNGG 4 cut(s) 787, 853, 894, 905
MvaI CCWGG 2 cut(s) 787, 894
MwoI GCNNNNNNNGC 4 cut(s) 251, 257, 260, 284
NciI CCSGG 2 cut(s) 853, 905
NdeII GATC 3 cut(s) 4, 163, 529
NlaIII CATG 7 cut(s) 73, 410, 459, 582, 668, 732, 763
NlaIV GGNNCC 4 cut(s) 349, 512, 513, 891
NmuCI GTSAC 5 cut(s) 371, 422, 458, 559, 581
NspI RCATGY 3 cut(s) 73, 459, 582
PaeI GCATGC 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 593
PceI AGGCCT 1 cut(s) 386
PciI ACATGT 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 903
PkrI GCNGC 5 cut(s) 253, 262, 359, 362, 487
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 511
PscI ACATGT 1 cut(s) 578
PshBI ATTAAT 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 511
Psp6I CCWGG 2 cut(s) 785, 892
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 2 cut(s) 785, 892
PspN4I GGNNCC 4 cut(s) 349, 512, 513, 891
PspPI GGNCC 6 cut(s) 217, 448, 511, 622, 869, 890
PspPPI RGGWCCY 1 cut(s) 511
PstI CTGCAG 2 cut(s) 335, 362
PstNI CAGNNNCTG 2 cut(s) 260, 563
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 4 cut(s) 143, 349, 445, 686
RsaNI GTAC 4 cut(s) 142, 348, 444, 685
RseI CAYNNNNRTG 1 cut(s) 764
SaqAI TTAA 7 cut(s) 96, 132, 159, 390, 399, 591, 925
SatI GCNGC 5 cut(s) 252, 261, 358, 361, 486
Sau3AI GATC 3 cut(s) 4, 163, 529
Sau96I GGNCC 6 cut(s) 217, 448, 511, 622, 869, 890
SchI GAGTC 1 cut(s) 420
ScrFI CCNGG 4 cut(s) 787, 853, 894, 905
SduI GDGCHC 1 cut(s) 820
SfcI CTRYAG 3 cut(s) 331, 358, 675
SinI GGWCC 6 cut(s) 217, 448, 511, 622, 869, 890
SmiMI CAYNNNNRTG 1 cut(s) 764
SpeI ACTAGT 1 cut(s) 199
SphI GCATGC 1 cut(s) 73
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 2 cut(s) 363, 804
SspMI CTAG 3 cut(s) 200, 224, 813
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 4 cut(s) 785, 851, 892, 903
TaaI ACNGT 2 cut(s) 472, 701
TaqI TCGA 2 cut(s) 180, 267
TaqII GACCGA 1 cut(s) 639
Tru1I TTAA 7 cut(s) 96, 132, 159, 390, 399, 591, 925
Tru9I TTAA 7 cut(s) 96, 132, 159, 390, 399, 591, 925
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 5 cut(s) 371, 422, 458, 559, 581
TseI GCWGC 5 cut(s) 251, 260, 357, 360, 485
Tsp45I GTSAC 5 cut(s) 371, 422, 458, 559, 581
TspDTI ATGAA 4 cut(s) 519, 537, 717, 776
TspGWI ACGGA 2 cut(s) 461, 837
TspRI CASTG 1 cut(s) 568
VpaK11BI GGWCC 6 cut(s) 217, 448, 511, 622, 869, 890
VspI ATTAAT 1 cut(s) 96
XapI RAATTY 2 cut(s) 587, 724
XceI RCATGY 3 cut(s) 73, 459, 582
XcmI CCANNNNNNNNNTGG 2 cut(s) 227, 287
XspI CTAG 3 cut(s) 200, 224, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.