RchiOBHm_Chr5g0043021

Belongs to the complex I subunit 6 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020902
Physical Location & Seq
Forward (+)
37821719 .. 37822249
531 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32135

Sequence Viewer

Length: 531 bp
ATGGATTTGGCTGGACCAATACATGATTTTCTTTTAGTCTTTCTGGGATCGGGTCTTATACTAGGAGGTATAGGTGTGGTATTATTTACCAACCCCATTTATTCTGCCTTTTCATTGGGACTTGTTCTTGTTTGTATATCCTTATTCTATATTCTATTAAACTCCTATTTTGTAGCCGCTGCACAACTTCTTATTTACGTGGGAGCTATAAATGTTTTAATTGTATTTGCTGTGATGTTCCTGAATGGTTCAGAGTATTACAAAGATTTTAATCTTTGGACTGTTGGGGATGGGATTACTTTGCCTGTTTGTACAAGTATTTTTGTTTTACTAATGATTACTATTACGGATACGTCATGGTACGGGATTATTTGGACTACAAGATCAAACCAGATTATAGAGCAAGATTTGATAAGTAATAGTCAACAAATTGGAATTCATTTATCAACCGATTTTTTTCTTTCATTTGAATTCATTTCGATAATTCTTTTAGTCGCTTTGATAGGCGCAATTGCCGTAGCGCGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0009987 GO:0015979 GO:0044237
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Oxidored_q3

Protein Analysis

176

Amino Acids

19.33

Weight (kDa)

4.2

Isoelectric Point (pI)

23.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q3 PF00499 23 - 172 8.8e-34 NADH-ubiquinone/plastoquinone oxidoreductase chain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020902)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01080
pyrus_communis pycom16g17780
rosa_chinensis RchiOBHm_Chr5g0017201 RchiOBHm_Chr5g0043021 RchiOBHm_CPg0502761

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 523
AciI CCGC 1 cut(s) 177
AclWI GGATC 1 cut(s) 55
AcsI RAATTY 2 cut(s) 435, 470
AfaI GTAC 2 cut(s) 313, 362
AgsI TTSAA 1 cut(s) 470
AluBI AGCT 1 cut(s) 206
AluI AGCT 1 cut(s) 206
AlwI GGATC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 2 cut(s) 435, 470
AspLEI GCGC 3 cut(s) 509, 523, 525
AspS9I GGNCC 1 cut(s) 14
AvaII GGWCC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 500
BciVI GTATCC 1 cut(s) 343
BfaI CTAG 1 cut(s) 62
BfuI GTATCC 1 cut(s) 343
BisI GCNGC 2 cut(s) 177, 180
BlsI GCNGC 2 cut(s) 178, 181
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 526
Bse8I GATNNNNATC 1 cut(s) 270
BseGI GGATG 1 cut(s) 295
BseJI GATNNNNATC 1 cut(s) 270
BseNI ACTGG 1 cut(s) 526
BsePI GCGCGC 1 cut(s) 521
BseXI GCAGC 1 cut(s) 166
BsgI GTGCAG 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 523
BslFI GGGAC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 132
Bsp1407I TGTACA 1 cut(s) 311
Bsp143I GATC 2 cut(s) 47, 383
BspACI CCGC 1 cut(s) 177
BspFNI CGCG 1 cut(s) 523
BspPI GGATC 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 311
BsrI ACTGG 1 cut(s) 526
BssHII GCGCGC 1 cut(s) 521
BssMI GATC 2 cut(s) 47, 383
Bst4CI ACNGT 1 cut(s) 283
BstAUI TGTACA 1 cut(s) 311
BstBAI YACGTR 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 523
BstF5I GGATG 1 cut(s) 295
BstFNI CGCG 1 cut(s) 523
BstHHI GCGC 3 cut(s) 509, 523, 525
BstKTI GATC 2 cut(s) 50, 386
BstMBI GATC 2 cut(s) 47, 383
BstUI CGCG 1 cut(s) 523
BstV1I GCAGC 1 cut(s) 166
BsuI GTATCC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 523
CfoI GCGC 3 cut(s) 509, 523, 525
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 312, 361
CviAII CATG 2 cut(s) 23, 357
CviJI RGCY 3 cut(s) 11, 176, 206
CviKI_1 RGCY 3 cut(s) 11, 176, 206
CviQI GTAC 2 cut(s) 312, 361
DpnI GATC 2 cut(s) 49, 385
DpnII GATC 2 cut(s) 47, 383
Eco47I GGWCC 1 cut(s) 14
EcoRI GAATTC 2 cut(s) 435, 470
FaeI CATG 2 cut(s) 26, 360
FaiI YATR 8 cut(s) 24, 59, 71, 137, 150, 209, 358, 398
FaqI GGGAC 1 cut(s) 132
FatI CATG 2 cut(s) 22, 356
Fnu4HI GCNGC 2 cut(s) 177, 180
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 2 cut(s) 177, 180
FspBI CTAG 1 cut(s) 62
GlaI GCGC 3 cut(s) 508, 522, 524
GluI GCNGC 2 cut(s) 177, 180
HhaI GCGC 3 cut(s) 509, 523, 525
Hin1II CATG 2 cut(s) 26, 360
Hin6I GCGC 3 cut(s) 507, 521, 523
HinP1I GCGC 3 cut(s) 507, 521, 523
HincII GTYRAC 1 cut(s) 425
HindII GTYRAC 1 cut(s) 425
Hpy166II GTNNAC 1 cut(s) 425
Hpy188I TCNGA 1 cut(s) 253
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 425
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 2 cut(s) 198, 353
HpyCH4V TGCA 1 cut(s) 182
HpySE526I ACGT 2 cut(s) 198, 353
Hsp92II CATG 2 cut(s) 26, 360
HspAI GCGC 3 cut(s) 507, 521, 523
Kzo9I GATC 2 cut(s) 47, 383
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 4 cut(s) 29, 254, 318, 404
Lsp1109I GCAGC 1 cut(s) 166
MaeI CTAG 1 cut(s) 62
MaeII ACGT 2 cut(s) 198, 353
MalI GATC 2 cut(s) 49, 385
MboI GATC 2 cut(s) 47, 383
MfeI CAATTG 1 cut(s) 510
MluCI AATT 6 cut(s) 219, 429, 435, 470, 483, 510
MnlI CCTC 1 cut(s) 59
MseI TTAA 3 cut(s) 158, 218, 270
MspA1I CMGCKG 1 cut(s) 179
MunI CAATTG 1 cut(s) 510
MvnI CGCG 1 cut(s) 523
NdeII GATC 2 cut(s) 47, 383
NlaIII CATG 2 cut(s) 26, 360
PauI GCGCGC 1 cut(s) 521
PkrI GCNGC 2 cut(s) 178, 181
Ppu21I YACGTR 1 cut(s) 199
PspPI GGNCC 1 cut(s) 14
PteI GCGCGC 1 cut(s) 521
RsaI GTAC 2 cut(s) 313, 362
RsaNI GTAC 2 cut(s) 312, 361
SaqAI TTAA 3 cut(s) 158, 218, 270
SatI GCNGC 2 cut(s) 177, 180
Sau3AI GATC 2 cut(s) 47, 383
Sau96I GGNCC 1 cut(s) 14
SetI ASST 5 cut(s) 70, 76, 201, 208, 356
SinI GGWCC 1 cut(s) 14
Sse9I AATT 6 cut(s) 219, 429, 435, 470, 483, 510
SsiI CCGC 1 cut(s) 177
SspMI CTAG 1 cut(s) 62
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 2 cut(s) 201, 356
TaqI TCGA 1 cut(s) 479
TasI AATT 6 cut(s) 219, 429, 435, 470, 483, 510
TatI WGTACW 1 cut(s) 311
TauI GCSGC 1 cut(s) 179
Tru1I TTAA 3 cut(s) 158, 218, 270
Tru9I TTAA 3 cut(s) 158, 218, 270
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 4 cut(s) 102, 428, 453, 463
TspGWI ACGGA 1 cut(s) 362
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 2 cut(s) 435, 470
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.