RchiOBHm_Chr5g0045911

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0022779
Physical Location & Seq
Reverse (-)
41954559 .. 41962561
8003 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32396

Sequence Viewer

Length: 360 bp
ATGTTATTAGGTTCTCATCTTTCATTTTTCTCTGTAACGAAGAGATCATTGCCATGGAAGTACCAGAAGATTACCCCTCGGCTGCTCCAGAAGTTGAAGATGAAGCTGTCATCAGTTAATGCATTACTTGAGGATGCAGTGGAGAAGCAACTTAAAAGATCGAGAGTGTGGGAATGGCTTGACGAGCTTAAAGATGCTCTCTATCATGCTGAGAACCTGCTGGATGACAACAATACTGAAGCTCTACGCCGCAAGCTGGAAGAAGATTCTGGAACCAGCAGAACAAGTCAGATGTTCAAGAAATTCACCAAAACAAAAGGATCTTTTCTCCTTGTTTTCATGCGTTTGAGAAAGCAGTGA

Protein Analysis

119

Amino Acids

14.11

Weight (kDa)

10.04

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 23 - 93 8.4e-16 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022779)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0045911
rosa_roxburghii Rroxscaffold_1G00035300 Rroxscaffold_1G00036380
rosa_samantha Rh5DG326000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 225
AciI CCGC 1 cut(s) 250
AclWI GGATC 1 cut(s) 328
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 2 cut(s) 97, 298
AluBI AGCT 4 cut(s) 106, 187, 242, 256
AluI AGCT 4 cut(s) 106, 187, 242, 256
AlwI GGATC 1 cut(s) 328
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 298
BbvI GCAGC 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 225
BisI GCNGC 2 cut(s) 83, 250
BlsI GCNGC 2 cut(s) 84, 251
BmiI GGNNCC 1 cut(s) 274
BmsI GCATC 2 cut(s) 124, 184
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 53, 77
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 47
BseDI CCNNGG 2 cut(s) 53, 77
BseGI GGATG 2 cut(s) 139, 229
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 256
Bsp143I GATC 3 cut(s) 44, 158, 320
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 1 cut(s) 250
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 225
BspPI GGATC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 53, 77
BssMI GATC 3 cut(s) 44, 158, 320
BssT1I CCWWGG 1 cut(s) 53
Bst6I CTCTTC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 53
BstF5I GGATG 2 cut(s) 139, 229
BstKTI GATC 3 cut(s) 47, 161, 323
BstMBI GATC 3 cut(s) 44, 158, 320
BstMWI GCNNNNNNNGC 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
BtgI CCRYGG 1 cut(s) 53
BtsCI GGATG 2 cut(s) 139, 229
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 61
CviAII CATG 3 cut(s) 54, 206, 340
CviJI RGCY 6 cut(s) 82, 106, 178, 187, 242, 256
CviKI_1 RGCY 6 cut(s) 82, 106, 178, 187, 242, 256
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 1 cut(s) 210
DpnI GATC 3 cut(s) 46, 160, 322
DpnII GATC 3 cut(s) 44, 158, 320
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 53
EcoT22I ATGCAT 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 3 cut(s) 57, 209, 343
FaiI YATR 3 cut(s) 55, 207, 341
FatI CATG 3 cut(s) 53, 205, 339
Fnu4HI GCNGC 2 cut(s) 83, 250
FokI GGATG 2 cut(s) 146, 236
Fsp4HI GCNGC 2 cut(s) 83, 250
GluI GCNGC 2 cut(s) 83, 250
GsuI CTGGAG 1 cut(s) 71
Hin1II CATG 3 cut(s) 57, 209, 343
HinfI GANTC 1 cut(s) 266
HphI GGTGA 1 cut(s) 298
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 4 cut(s) 88, 162, 270, 298
HpyCH4V TGCA 2 cut(s) 122, 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 184
HpyF3I CTNAG 1 cut(s) 210
Hsp92II CATG 3 cut(s) 57, 209, 343
Kzo9I GATC 3 cut(s) 44, 158, 320
LmnI GCTCC 1 cut(s) 90
LpnPI CCDG 7 cut(s) 77, 101, 206, 230, 242, 255, 289
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 2 cut(s) 124, 184
MaeIII GTNAC 1 cut(s) 34
MalI GATC 3 cut(s) 46, 160, 322
MboI GATC 3 cut(s) 44, 158, 320
MboII GAAGA 5 cut(s) 52, 79, 109, 272, 275
MflI RGATCY 1 cut(s) 320
MluCI AATT 1 cut(s) 302
MnlI CCTC 2 cut(s) 87, 124
Mph1103I ATGCAT 1 cut(s) 124
MseI TTAA 3 cut(s) 117, 153, 189
MslI CAYNNNNRTG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 184
NcoI CCATGG 1 cut(s) 53
NdeII GATC 3 cut(s) 44, 158, 320
NlaIII CATG 3 cut(s) 57, 209, 343
NlaIV GGNNCC 1 cut(s) 274
NmeAIII GCCGAG 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 124
PfeI GAWTC 1 cut(s) 266
PkrI GCNGC 2 cut(s) 84, 251
PspN4I GGNNCC 1 cut(s) 274
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 52
SaqAI TTAA 3 cut(s) 117, 153, 189
SatI GCNGC 2 cut(s) 83, 250
Sau3AI GATC 3 cut(s) 44, 158, 320
SetI ASST 6 cut(s) 13, 108, 189, 219, 244, 258
SfaNI GCATC 2 cut(s) 124, 184
SmiMI CAYNNNNRTG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 302
SsiI CCGC 1 cut(s) 250
StyI CCWWGG 1 cut(s) 53
TaqI TCGA 1 cut(s) 161
TasI AATT 1 cut(s) 302
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 3 cut(s) 117, 153, 189
Tru9I TTAA 3 cut(s) 117, 153, 189
TscAI CASTG 1 cut(s) 144
TseI GCWGC 1 cut(s) 82
TspDTI ATGAA 3 cut(s) 12, 116, 328
TspRI CASTG 1 cut(s) 144
XapI RAATTY 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.