RchiOBHm_Chr6g0246921
NBS-LRR Family

Disease resistance protein (TIR-NBS-LRR class)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020572
Physical Location & Seq
Forward (+)
2635951 .. 2636586
636 bp
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UTR
Exon/CDS
Intron
PRQ22133

Sequence Viewer

Length: 636 bp
ATGGATTTTGCAAGCAGTGTACGTGGCTTAAAATCTCTTAAAGTTCTGAATCTTTCTGGTTGTTCAAAGATTAAAAAACTACCGAATGACATGGGTCACTTAGAAAGTTTGGAGGAGCTTCATGTGAATGGCACGGGCATAAGAGAACTACCTTCCTCTATTGGAATGCTAGAAAGACTTCCTCTACTGAAAATAGAAGATTGCAAAGATCTTGCGTGTCTTCCAATTAATCTAGGTGGCTTAAAATCTCTCAAGGTTGTTAATATTTCTGGTTGCTCAAATCTTGAAAAATTGCCAGAGGAGTTGGGCAATATAGAGTGGTTGGTAGAAGTTGATGTGAGTAGAACTTCTATACGAGAACTGCCTTGCTCTATTGGGATGCTTGAAGGACTTGTTTCTATGTCCTTGAGAGATTGCAAACATCTTGTGTGTCTTCCAAGAAGTGTGGGTGGCTTAAAATCTCTCAAAAATCTCAGTCTTTCTGGTTGCTTAAAGCTTGACAAATTGCTGGATGAGTTGGGTCATGTCAGTGGTGGACCCAGAATTTTATTTGTCTGGGGGCGTAAAAAAAAAAAATTAAACAATAAACTTGAAAACAATTTGTTGTTTATCGTAACAATTCGAATGAGTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0042742 GO:0043207 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 NB-ARC TIR

Protein Analysis

211

Amino Acids

23.17

Weight (kDa)

9.02

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 7 - 89 1.2e-13 Leucine-rich repeat region
LRR_13 PF23286 10 - 95 1.4e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 78 - 197 7.7e-12 Leucine-rich repeat region
LRR_13 PF23286 81 - 167 7.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020572)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0246921 RchiOBHm_Chr6g0249181 RchiOBHm_Chr6g0249741
rosa_multiflora Rmu_co8074252.1_g000001 Rmu_sc0005257.1_g000015
rosa_roxburghii Rroxscaffold_6G00392290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 543
AfaI GTAC 2 cut(s) 21, 631
AgsI TTSAA 4 cut(s) 66, 287, 386, 593
AluBI AGCT 2 cut(s) 118, 496
AluI AGCT 2 cut(s) 118, 496
ApoI RAATTY 1 cut(s) 543
AseI ATTAAT 1 cut(s) 228
Asp700I GAANNNNTTC 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 536
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 1 cut(s) 536
BbsI GAAGAC 2 cut(s) 212, 425
BfaI CTAG 2 cut(s) 170, 233
BglII AGATCT 1 cut(s) 208
BmcAI AGTACT 1 cut(s) 631
Bme18I GGWCC 1 cut(s) 536
BmgT120I GGNCC 1 cut(s) 536
BmiI GGNNCC 1 cut(s) 538
BmsI GCATC 1 cut(s) 369
BoxI GACNNNNGTC 1 cut(s) 93
BpiI GAAGAC 2 cut(s) 212, 425
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 2 cut(s) 236, 427
BsaAI YACGTR 1 cut(s) 23
BseGI GGATG 2 cut(s) 384, 517
BseMII CTCAG 1 cut(s) 487
BseRI GAGGAG 2 cut(s) 128, 314
BsmI GAATGC 1 cut(s) 171
Bsp119I TTCGAA 1 cut(s) 622
Bsp143I GATC 1 cut(s) 208
BspCNI CTCAG 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 538
BspT104I TTCGAA 1 cut(s) 622
BssMI GATC 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 23
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 3 cut(s) 100, 473, 633
BstF5I GGATG 2 cut(s) 384, 517
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstPAI GACNNNNGTC 1 cut(s) 93
BstV2I GAAGAC 2 cut(s) 212, 425
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BtsCI GGATG 2 cut(s) 384, 517
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 22, 535
Cac8I GCNNGC 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 536
Csp6I GTAC 2 cut(s) 20, 630
CspCI CAANNNNNGTGG 2 cut(s) 426, 461
CviAII CATG 3 cut(s) 91, 122, 524
CviJI RGCY 5 cut(s) 27, 118, 240, 453, 496
CviKI_1 RGCY 5 cut(s) 27, 118, 240, 453, 496
CviQI GTAC 2 cut(s) 20, 630
DdeI CTNAG 3 cut(s) 100, 473, 633
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 536
FaeI CATG 3 cut(s) 94, 125, 527
FaiI YATR 7 cut(s) 92, 123, 140, 314, 353, 401, 525
FatI CATG 3 cut(s) 90, 121, 523
FokI GGATG 2 cut(s) 391, 524
FspBI CTAG 2 cut(s) 170, 233
Hin1II CATG 3 cut(s) 94, 125, 527
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 20, 536
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 2 cut(s) 20, 536
HpyAV CCTTC 2 cut(s) 162, 380
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 3 cut(s) 11, 204, 417
HpyF3I CTNAG 3 cut(s) 100, 473, 633
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 3 cut(s) 94, 125, 527
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 7 cut(s) 42, 255, 309, 468, 494, 541, 553
LweI GCATC 1 cut(s) 369
MaeI CTAG 2 cut(s) 170, 233
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 2 cut(s) 95, 613
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 3 cut(s) 209, 212, 425
MflI RGATCY 1 cut(s) 208
MluCI AATT 7 cut(s) 225, 290, 503, 543, 575, 598, 618
MnlI CCTC 4 cut(s) 106, 166, 192, 292
MroXI GAANNNNTTC 1 cut(s) 177
MseI TTAA 9 cut(s) 29, 39, 72, 228, 242, 261, 455, 491, 578
MslI CAYNNNNRTG 2 cut(s) 126, 528
Mva1269I GAATGC 1 cut(s) 171
NdeII GATC 1 cut(s) 208
NlaIII CATG 3 cut(s) 94, 125, 527
NlaIV GGNNCC 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 95
NspV TTCGAA 1 cut(s) 622
PctI GAATGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 1 cut(s) 49
Ppu21I YACGTR 1 cut(s) 23
PshAI GACNNNNGTC 1 cut(s) 93
PshBI ATTAAT 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 1 cut(s) 536
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 2 cut(s) 21, 631
RsaNI GTAC 2 cut(s) 20, 630
RseI CAYNNNNRTG 2 cut(s) 126, 528
SaqAI TTAA 9 cut(s) 29, 39, 72, 228, 242, 261, 455, 491, 578
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 1 cut(s) 536
ScaI AGTACT 1 cut(s) 631
SetI ASST 6 cut(s) 25, 120, 154, 238, 258, 498
SfaNI GCATC 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 622
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 2 cut(s) 126, 528
SmlI CTYRAG 2 cut(s) 251, 406
SmoI CTYRAG 2 cut(s) 251, 406
Sse9I AATT 7 cut(s) 225, 290, 503, 543, 575, 598, 618
SspI AATATT 1 cut(s) 265
SspMI CTAG 2 cut(s) 170, 233
TaiI ACGT 1 cut(s) 25
TaqI TCGA 1 cut(s) 622
TasI AATT 7 cut(s) 225, 290, 503, 543, 575, 598, 618
TatI WGTACW 1 cut(s) 629
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 9 cut(s) 29, 39, 72, 228, 242, 261, 455, 491, 578
Tru9I TTAA 9 cut(s) 29, 39, 72, 228, 242, 261, 455, 491, 578
TscAI CASTG 2 cut(s) 22, 535
TseFI GTSAC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 1 cut(s) 110
TspRI CASTG 2 cut(s) 22, 535
VpaK11BI GGWCC 1 cut(s) 536
VspI ATTAAT 1 cut(s) 228
XapI RAATTY 1 cut(s) 543
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 2 cut(s) 170, 233
ZrmI AGTACT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.