RchiOBHm_Chr6g0249181

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020572
Physical Location & Seq
Reverse (-)
5031169 .. 5031945
777 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22339

Sequence Viewer

Length: 777 bp
ATGCCACATCTTGAGGTTCTGTTTCTAGAAGGTTGTACAAGATTATATGAGGTTGACCCAGGGGTTGAAGTGCTTGAAAAACTTACGATGCTGAGTTTAAAAGATTGCAAGAATCTCGTGCATTTTGCAAGCAGTGTACGTGGCTTAAAATCTCTCGAAGTTCTTAATCTTTCTGGTTGCTCAAAGCTTAGAAAACTACCAAATGACATTGGTCATTTAGAAAGTTTGGTCGAGGTTGATGTGAGTGGAACTTCTATACGAGAACTACCTTGCTCAATTGGCATGCTTGAACGACTTGTTTCTATGTCCTTGAGAGATTGTAAACATCTTGTGTGTCTTCCAAGTAGTGTAGGAATCTTAAAATCTCTCAAAGATCTCAATATTTCTGGTTGCTCAAAGCTTGACAAATTGCCAAATGAGTTGGGCCATATAGCTTGTTTGGAAGAGGTTGATGCGAGTGGGACTTCTATACGAGAGATACCTTGCTCTATTGGCATGCTTGAAGGACTTGTTTCTATGTCCTTGAGAGATTGCAAAAATCTTGTGTGTCTTCCAAGCAGTGTAGGTGGCTTAAAATCTCTCAAAGATCTCAATCTTTCTGGTTGCTCAAAGCTTGATAAATTGTCAGATGAGTTGGGTCGTGTTGCCTGTTTGGAGAAGCTTGATGTGAGTGGAAGTGGCATAAGAGAAGTGCCATCCTCTATTGGTCTTTTGAAAAACCTCAAAAAATTATTTCTTGGAGGATGTATGGACAATCACATAAATCATGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

258

Amino Acids

27.81

Weight (kDa)

6.39

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 26 - 129 3.8e-13 Leucine-rich repeat region
LRR_13 PF23286 49 - 135 1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 123 - 206 6.8e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 133 - 200 3.8e-08 Leucine-rich repeat region
LRR_14 PF23598 198 - 249 3.2e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020572)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0246921 RchiOBHm_Chr6g0249181 RchiOBHm_Chr6g0249741
rosa_multiflora Rmu_co8074252.1_g000001 Rmu_sc0005257.1_g000015
rosa_roxburghii Rroxscaffold_6G00392290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 37, 138
AgsI TTSAA 5 cut(s) 68, 77, 290, 503, 715
AjnI CCWGG 1 cut(s) 58
AluBI AGCT 5 cut(s) 187, 400, 434, 613, 661
AluI AGCT 5 cut(s) 187, 400, 434, 613, 661
AoxI GGCC 1 cut(s) 424
AspS9I GGNCC 1 cut(s) 424
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 329, 542
BccI CCATC 1 cut(s) 703
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BciT130I CCWGG 1 cut(s) 60
BfaI CTAG 1 cut(s) 26
BglII AGATCT 2 cut(s) 373, 586
Bme1390I CCNGG 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 424
BmrFI CCNGG 1 cut(s) 60
BmsI GCATC 2 cut(s) 78, 442
BoxI GACNNNNGTC 1 cut(s) 210
BpiI GAAGAC 2 cut(s) 329, 542
BpuEI CTTGAG 3 cut(s) 32, 331, 544
BsaAI YACGTR 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 591
BsaJI CCNNGG 2 cut(s) 58, 59
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 2 cut(s) 58, 59
BseGI GGATG 2 cut(s) 695, 749
BseJI GATNNNNATC 1 cut(s) 591
BseMII CTCAG 1 cut(s) 83
BshFI GGCC 1 cut(s) 426
BslFI GGGAC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 1 cut(s) 426
Bsp1407I TGTACA 1 cut(s) 35
Bsp143I GATC 2 cut(s) 373, 586
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 58, 59
BssMI GATC 2 cut(s) 373, 586
BssSI CACGAG 1 cut(s) 116
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 60
Bst6I CTCTTC 1 cut(s) 438
BstAUI TGTACA 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 3 cut(s) 130, 284, 497
BstDEI CTNAG 2 cut(s) 92, 188
BstF5I GGATG 2 cut(s) 695, 749
BstKTI GATC 2 cut(s) 376, 589
BstMBI GATC 2 cut(s) 373, 586
BstMWI GCNNNNNNNGC 2 cut(s) 279, 492
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 2 cut(s) 286, 499
BstPAI GACNNNNGTC 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 58
BstV2I GAAGAC 2 cut(s) 329, 542
BstX2I RGATCY 2 cut(s) 373, 586
BstYI RGATCY 2 cut(s) 373, 586
BsuRI GGCC 1 cut(s) 426
BtsCI GGATG 2 cut(s) 695, 749
BtsI GCAGTG 2 cut(s) 139, 565
BtsIMutI CAGTG 2 cut(s) 139, 565
Cac8I GCNNGC 3 cut(s) 130, 284, 497
Cfr13I GGNCC 1 cut(s) 424
Csp6I GTAC 2 cut(s) 36, 137
CviAII CATG 3 cut(s) 283, 496, 767
CviJI RGCY 8 cut(s) 144, 187, 400, 426, 434, 570, 613, 661
CviKI_1 RGCY 8 cut(s) 144, 187, 400, 426, 434, 570, 613, 661
CviQI GTAC 2 cut(s) 36, 137
DdeI CTNAG 2 cut(s) 92, 188
DpnI GATC 2 cut(s) 375, 588
DpnII GATC 2 cut(s) 373, 586
DraI TTTAAA 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 58
FaeI CATG 3 cut(s) 286, 499, 770
FaqI GGGAC 1 cut(s) 475
FatI CATG 3 cut(s) 282, 495, 766
FokI GGATG 2 cut(s) 682, 756
FspBI CTAG 1 cut(s) 26
HaeIII GGCC 1 cut(s) 426
Hin1II CATG 3 cut(s) 286, 499, 770
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 4 cut(s) 185, 398, 611, 659
HinfI GANTC 2 cut(s) 112, 354
Hpy166II GTNNAC 3 cut(s) 55, 137, 323
Hpy188I TCNGA 1 cut(s) 628
Hpy188III TCNNGA 3 cut(s) 11, 26, 155
Hpy8I GTNNAC 3 cut(s) 55, 137, 323
HpyAV CCTTC 2 cut(s) 23, 497
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 4 cut(s) 108, 121, 128, 534
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 492
HpyF3I CTNAG 2 cut(s) 92, 188
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 3 cut(s) 286, 499, 770
Kzo9I GATC 2 cut(s) 373, 586
LpnPI CCDG 6 cut(s) 45, 72, 159, 372, 585, 661
LweI GCATC 2 cut(s) 78, 442
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 139
MalI GATC 2 cut(s) 375, 588
MboI GATC 2 cut(s) 373, 586
MboII GAAGA 3 cut(s) 329, 455, 542
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 2 cut(s) 373, 586
MluCI AATT 4 cut(s) 276, 407, 620, 728
MnlI CCTC 7 cut(s) 7, 43, 226, 439, 709, 731, 734
MseI TTAA 5 cut(s) 98, 146, 165, 359, 572
MspR9I CCNGG 1 cut(s) 60
MunI CAATTG 1 cut(s) 276
MvaI CCWGG 1 cut(s) 60
MwoI GCNNNNNNNGC 2 cut(s) 279, 492
NdeII GATC 2 cut(s) 373, 586
NlaIII CATG 3 cut(s) 286, 499, 770
NspI RCATGY 2 cut(s) 286, 499
PaeI GCATGC 2 cut(s) 286, 499
PasI CCCWGGG 1 cut(s) 59
PfeI GAWTC 2 cut(s) 112, 354
Ppu21I YACGTR 1 cut(s) 140
PshAI GACNNNNGTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspPI GGNCC 1 cut(s) 424
PsuI RGATCY 2 cut(s) 373, 586
RsaI GTAC 2 cut(s) 37, 138
RsaNI GTAC 2 cut(s) 36, 137
SaqAI TTAA 5 cut(s) 98, 146, 165, 359, 572
Sau3AI GATC 2 cut(s) 373, 586
Sau96I GGNCC 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 60
SfaNI GCATC 2 cut(s) 78, 442
SmlI CTYRAG 3 cut(s) 11, 310, 523
SmoI CTYRAG 3 cut(s) 11, 310, 523
SphI GCATGC 2 cut(s) 286, 499
Sse9I AATT 4 cut(s) 276, 407, 620, 728
SspI AATATT 1 cut(s) 382
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 58
TaiI ACGT 1 cut(s) 142
TaqI TCGA 2 cut(s) 156, 231
TasI AATT 4 cut(s) 276, 407, 620, 728
TatI WGTACW 1 cut(s) 35
TfiI GAWTC 2 cut(s) 112, 354
Tru1I TTAA 5 cut(s) 98, 146, 165, 359, 572
Tru9I TTAA 5 cut(s) 98, 146, 165, 359, 572
TscAI CASTG 2 cut(s) 139, 565
TspRI CASTG 2 cut(s) 139, 565
XbaI TCTAGA 1 cut(s) 25
XceI RCATGY 2 cut(s) 286, 499
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.