RchiOBHm_Chr6g0251611

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0025072
Physical Location & Seq
Reverse (-)
6865197 .. 6866297
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22556

Sequence Viewer

Length: 849 bp
ATGTTTACCGTCCTCCTTTTCCTCGTTCTCTTTGTTTCTCTTCTTCTTCAGTTTCTTAATTATCTTCTACCACCACCTGCCCAATCTCCAGCAGATTCCCCGAACATCCAAATACCTGCATCTCCTTCAAACCCTAAATCAGAACATGAGGAAGTACCAGCGGATTCTCCGAACATAAAAATACCTGCATCTCCTTCAAACCCTAAATCAGAACATGAGGAAGTTCCAGCGGATTCCCGGAACATCCAAATACCTGCATCTCCTTCAAACCCTAAATTAGAACATGTGGAAGTTCCAGTGGATTCCACAAACACCCAAACAGTGCTTTCATCTCTTTCAGCCCTAATGAAACATGACAAAATTCCAGCAGAAGCTTCCTCTTCTTCGGGCTCAACATTTGATGTCTTCCTCAGTTTTAGAGGTGAAGACACCCGCCATGGTTTTACTGACCATTTGTATTATGCTATGATTCAGAAAGGAATCATTGCATTCAGGGATACAGAAAAACTCGAGAGGGGACGTCCAATTTCATCAGATCTACTGAAAGCAATAAAAGAATCCCGAATTGCAGTCATCATTCTTTCAGCTAACTATGCTACTTCAACATGGTGCTTGGAGGAGCTTGCACAGATTGTTGAATGCGAAAAGTCAACGGGGTTGAAAGTTTTACCAGTCTTTTATCACGTCGAACCATCAGAAGTAAGGAAGCAAACAGGAAGTTTTTACAAGGCGTTTTGCAAGCATGCAAAAACTTTCGCAGACGACATGGGGAAGGCGAAGGTGAACAAGTGGAGAGAAGCTTTAACCAAAGTAGCCAATATCTCCGGATGGGATTTGAAAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

282

Amino Acids

31.21

Weight (kDa)

6.46

Isoelectric Point (pI)

57.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 133 - 279 1.2e-50 TIR domain
TIR_2 PF13676 135 - 231 3.4e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0025072)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0251611
rosa_laevigata RLG00000015455 RLG00000015456

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 85
AatII GACGTC 1 cut(s) 523
Acc36I ACCTGC 4 cut(s) 85, 124, 193, 262
AccIII TCCGGA 1 cut(s) 824
AciI CCGC 3 cut(s) 161, 230, 433
AcsI RAATTY 1 cut(s) 360
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 520
AfaI GTAC 1 cut(s) 156
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 7 cut(s) 129, 198, 267, 603, 638, 661, 838
AjiI CACGTC 1 cut(s) 685
AluBI AGCT 4 cut(s) 374, 587, 622, 800
AluI AGCT 4 cut(s) 374, 587, 622, 800
Ama87I CYCGRG 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 824
ApoI RAATTY 1 cut(s) 360
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 2 cut(s) 434, 793
AvaI CYCGRG 1 cut(s) 509
BanII GRGCYC 1 cut(s) 392
BbsI GAAGAC 2 cut(s) 397, 432
BccI CCATC 2 cut(s) 700, 822
BciVI GTATCC 1 cut(s) 490
BcnI CCSGG 1 cut(s) 238
BfuAI ACCTGC 4 cut(s) 85, 124, 193, 262
BfuI GTATCC 1 cut(s) 490
BglII AGATCT 1 cut(s) 535
Bme1390I CCNGG 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 509
BmgBI CACGTC 1 cut(s) 685
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 3 cut(s) 128, 197, 266
BpiI GAAGAC 2 cut(s) 397, 432
BpmI CTGGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 520
BsaJI CCNNGG 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 824
Bse1I ACTGG 2 cut(s) 296, 671
Bse3DI GCAATG 1 cut(s) 483
BseAI TCCGGA 1 cut(s) 824
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 3 cut(s) 105, 243, 833
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 424
BseNI ACTGG 2 cut(s) 296, 671
BseRI GAGGAG 1 cut(s) 632
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 238, 825
BslFI GGGAC 1 cut(s) 531
BsmFI GGGAC 1 cut(s) 531
BsmI GAATGC 2 cut(s) 488, 644
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 392
Bsp13I TCCGGA 1 cut(s) 824
Bsp143I GATC 1 cut(s) 535
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 3 cut(s) 161, 230, 433
BspCNI CTCAG 1 cut(s) 423
BspEI TCCGGA 1 cut(s) 824
BspMI ACCTGC 4 cut(s) 85, 124, 193, 262
BsrDI GCAATG 1 cut(s) 483
BsrI ACTGG 2 cut(s) 296, 671
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 1 cut(s) 535
BssNI GRCGYC 1 cut(s) 520
BssT1I CCWWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 10, 322
Bst6I CTCTTC 2 cut(s) 45, 385
BstACI GRCGYC 1 cut(s) 520
BstC8I GCNNGC 3 cut(s) 624, 740, 744
BstDEI CTNAG 1 cut(s) 410
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 3 cut(s) 105, 243, 833
BstKTI GATC 1 cut(s) 538
BstMBI GATC 1 cut(s) 535
BstMWI GCNNNNNNNGC 1 cut(s) 593
BstNSI RCATGY 2 cut(s) 287, 746
BstSCI CCNGG 1 cut(s) 236
BstV2I GAAGAC 2 cut(s) 397, 432
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BsuI GTATCC 1 cut(s) 490
BtgI CCRYGG 1 cut(s) 436
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 3 cut(s) 105, 243, 833
BtsIMutI CAGTG 2 cut(s) 303, 327
BveI ACCTGC 4 cut(s) 85, 124, 193, 262
Cac8I GCNNGC 3 cut(s) 624, 740, 744
Csp6I GTAC 1 cut(s) 155
CviAII CATG 8 cut(s) 146, 215, 284, 353, 437, 606, 743, 766
CviJI RGCY 7 cut(s) 341, 374, 390, 587, 622, 800, 815
CviKI_1 RGCY 7 cut(s) 341, 374, 390, 587, 622, 800, 815
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 1 cut(s) 410
DpnI GATC 1 cut(s) 537
DpnII GATC 1 cut(s) 535
Eam1104I CTCTTC 2 cut(s) 45, 385
EarI CTCTTC 2 cut(s) 45, 385
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 509
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 8 cut(s) 149, 218, 287, 356, 440, 609, 746, 769
FaqI GGGAC 1 cut(s) 531
FatI CATG 8 cut(s) 145, 214, 283, 352, 436, 605, 742, 765
FauI CCCGC 1 cut(s) 440
FokI GGATG 3 cut(s) 92, 230, 840
FriOI GRGCYC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 72
HapII CCGG 2 cut(s) 238, 825
Hin1I GRCGYC 1 cut(s) 520
Hin1II CATG 8 cut(s) 149, 218, 287, 356, 440, 609, 746, 769
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HindIII AAGCTT 2 cut(s) 372, 798
HinfI GANTC 7 cut(s) 95, 164, 233, 302, 469, 480, 557
HpaII CCGG 2 cut(s) 238, 825
HphI GGTGA 2 cut(s) 434, 793
Hpy166II GTNNAC 3 cut(s) 6, 651, 784
Hpy188I TCNGA 6 cut(s) 142, 171, 211, 474, 535, 697
Hpy188III TCNNGA 3 cut(s) 511, 561, 825
Hpy8I GTNNAC 3 cut(s) 6, 651, 784
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 6 cut(s) 135, 204, 273, 766, 772, 837
HpyCH4III ACNGT 2 cut(s) 10, 322
HpyCH4IV ACGT 2 cut(s) 520, 684
HpyCH4V TGCA 8 cut(s) 119, 188, 257, 488, 569, 626, 738, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 593
HpyF3I CTNAG 1 cut(s) 410
HpySE526I ACGT 2 cut(s) 520, 684
Hsp92I GRCGYC 1 cut(s) 520
Hsp92II CATG 8 cut(s) 149, 218, 287, 356, 440, 609, 746, 769
Kpn2I TCCGGA 1 cut(s) 824
Kzo9I GATC 1 cut(s) 535
LmnI GCTCC 1 cut(s) 619
LweI GCATC 3 cut(s) 128, 197, 266
MaeII ACGT 2 cut(s) 520, 684
MalI GATC 1 cut(s) 537
MboI GATC 1 cut(s) 535
MboII GAAGA 8 cut(s) 32, 35, 38, 56, 372, 375, 397, 437
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 5 cut(s) 58, 275, 360, 525, 564
MnlI CCTC 9 cut(s) 23, 32, 142, 211, 388, 413, 419, 507, 610
MroI TCCGGA 1 cut(s) 824
MseI TTAA 2 cut(s) 57, 803
MspA1I CMGCKG 2 cut(s) 161, 230
MspI CCGG 2 cut(s) 238, 825
MspR9I CCNGG 1 cut(s) 238
Mva1269I GAATGC 2 cut(s) 488, 644
MwoI GCNNNNNNNGC 1 cut(s) 593
NciI CCSGG 1 cut(s) 238
NcoI CCATGG 1 cut(s) 436
NdeII GATC 1 cut(s) 535
NlaIII CATG 8 cut(s) 149, 218, 287, 356, 440, 609, 746, 769
NspI RCATGY 2 cut(s) 287, 746
PaeI GCATGC 1 cut(s) 746
PaeR7I CTCGAG 1 cut(s) 509
PaqCI CACCTGC 1 cut(s) 85
PciI ACATGT 1 cut(s) 283
PctI GAATGC 2 cut(s) 488, 644
PfeI GAWTC 7 cut(s) 95, 164, 233, 302, 469, 480, 557
PfoI TCCNGGA 1 cut(s) 236
PscI ACATGT 1 cut(s) 283
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SaqAI TTAA 2 cut(s) 57, 803
Sau3AI GATC 1 cut(s) 535
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 1 cut(s) 392
SfaNI GCATC 3 cut(s) 128, 197, 266
Sfr274I CTCGAG 1 cut(s) 509
SlaI CTCGAG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
SphI GCATGC 1 cut(s) 746
Sse9I AATT 5 cut(s) 58, 275, 360, 525, 564
SsiI CCGC 3 cut(s) 161, 230, 433
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 10, 322
TaiI ACGT 2 cut(s) 523, 687
TaqI TCGA 2 cut(s) 510, 687
TasI AATT 5 cut(s) 58, 275, 360, 525, 564
TfiI GAWTC 7 cut(s) 95, 164, 233, 302, 469, 480, 557
Tru1I TTAA 2 cut(s) 57, 803
Tru9I TTAA 2 cut(s) 57, 803
TscAI CASTG 2 cut(s) 303, 327
TspDTI ATGAA 3 cut(s) 318, 362, 519
TspRI CASTG 2 cut(s) 303, 327
XapI RAATTY 1 cut(s) 360
XceI RCATGY 2 cut(s) 287, 746
XhoI CTCGAG 1 cut(s) 509
ZraI GACGTC 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.