RchiOBHm_Chr6g0271941

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020290
Physical Location & Seq
Forward (+)
31968766 .. 31969434
669 bp
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UTR
Exon/CDS
Intron
PRQ24393

Sequence Viewer

Length: 669 bp
ATGATCATTGACGGGAATCACATATCCCATGAAAGCCACTGCCACCAGCTCAAACTCGAGGACACAAAATTCCCGTTCAGGTGTGATGGCTGCAAGATGATAGGAATGGGCCAGCGCTACACTTGCGCCGCATGTAACTTCGACCTCCACAAGCACTGCGGCGTCGATCCTCATACTCCTATGTTCCACCCGCTGATGCCCAAGTGCAAAAACTTGAAGTTCTGCTTGAGGCCGCCGAAGAAAGCCACGCAATATTGCGATGCATGCACCAAACCTGTAACCGGGTTTTTGTACCGTTGTGAGGGGTGCGATTTCGACCTCCATCCCTGCTGCGCAAACCTTCCTATGGAGCTCGAGCATGAGGGTCTCAAGCTGAAGCTGATCGAGAAAGACGGCAGGTGGATTTATAGGTCGAGATGCAAGAAATATAAGTTGGAAGTAGCATATGCGAGGGAAATAATCGAGGAGAGCTCGACTGAACTTATGGGGTCAGAGATGACAAGCGTTCCGAAGATCAAGAATATGGTTCAGACCGGTCACAGCAGAGGTAAGGTGAGGAGGTATTGTGAGATGGTTGGGTTGGCTCTGAAGATTGTGATATCAGCATTGCTGGGTGATCCGACGAGTTTGATAATTGCGGTTATTGCTTCTTTCATGCGACCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

222

Amino Acids

25.24

Weight (kDa)

8.74

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 12 - 53 3.7e-07 C1 domain
C1_2 PF03107 71 - 112 2.3e-07 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020290)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07740
rosa_chinensis RchiOBHm_Chr6g0262071 RchiOBHm_Chr6g0271941
rosa_laevigata RLG00000014457
rosa_samantha Rh6CG106200 Rh6DG096600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 387
Acc16I TGCGCA 1 cut(s) 334
Acc36I ACCTGC 1 cut(s) 387
AciI CCGC 5 cut(s) 129, 159, 191, 233, 638
AclWI GGATC 2 cut(s) 161, 611
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 2 cut(s) 395, 608
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 1 cut(s) 293
AfeI AGCGCT 1 cut(s) 116
AfiI CCNNNNNNNGG 3 cut(s) 281, 301, 346
AgeI ACCGGT 1 cut(s) 533
AgsI TTSAA 1 cut(s) 217
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 5 cut(s) 49, 352, 373, 379, 471
AluI AGCT 5 cut(s) 49, 352, 373, 379, 471
Alw21I GWGCWC 2 cut(s) 354, 473
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 2 cut(s) 161, 611
Ama87I CYCGRG 2 cut(s) 56, 353
Aor51HI AGCGCT 1 cut(s) 116
AoxI GGCC 2 cut(s) 109, 230
ApeKI GCWGC 2 cut(s) 90, 330
ApoI RAATTY 1 cut(s) 68
AsiGI ACCGGT 1 cut(s) 533
AspLEI GCGC 3 cut(s) 117, 128, 335
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 565, 626
AvaI CYCGRG 2 cut(s) 56, 353
BanII GRGCYC 2 cut(s) 354, 473
Bbv12I GWGCWC 2 cut(s) 354, 473
BbvI GCAGC 2 cut(s) 77, 317
BccI CCATC 3 cut(s) 80, 330, 565
BceAI ACGGC 1 cut(s) 409
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 371
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 387
BisI GCNGC 5 cut(s) 91, 129, 160, 233, 331
BlsI GCNGC 5 cut(s) 92, 130, 161, 234, 332
Bme1390I CCNGG 1 cut(s) 283
BmeT110I CYCGRG 2 cut(s) 56, 353
BmgT120I GGNCC 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 3 cut(s) 186, 250, 407
BplI GAGNNNNNCTC 2 cut(s) 455, 487
BpuEI CTTGAG 2 cut(s) 247, 353
BpuMI CCSGG 1 cut(s) 283
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 533
BsaXI ACNNNNNCTCC 2 cut(s) 550, 580
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 301, 346
Bse118I RCCGGY 1 cut(s) 533
Bse3DI GCAATG 1 cut(s) 605
BseGI GGATG 2 cut(s) 322, 662
BseLI CCNNNNNNNGG 3 cut(s) 281, 301, 346
BseMI GCAATG 1 cut(s) 605
BseRI GAGGAG 2 cut(s) 479, 571
BseXI GCAGC 2 cut(s) 77, 317
BseYI CCCAGC 1 cut(s) 610
BshFI GGCC 2 cut(s) 111, 232
BshTI ACCGGT 1 cut(s) 533
BsiHKAI GWGCWC 2 cut(s) 354, 473
BsiHKCI CYCGRG 2 cut(s) 56, 353
BsiSI CCGG 2 cut(s) 282, 534
BslI CCNNNNNNNGG 3 cut(s) 281, 301, 346
BsmAI GTCTC 1 cut(s) 371
BsnI GGCC 2 cut(s) 111, 232
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 2 cut(s) 56, 353
Bsp1286I GDGCHC 2 cut(s) 354, 473
Bsp143I GATC 5 cut(s) 3, 166, 381, 513, 616
BspACI CCGC 5 cut(s) 129, 159, 191, 233, 638
BspANI GGCC 2 cut(s) 111, 232
BspMI ACCTGC 1 cut(s) 387
BspPI GGATC 2 cut(s) 161, 611
BspTNI GGTCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 605
BsrFI RCCGGY 1 cut(s) 533
BssAI RCCGGY 1 cut(s) 533
BssMI GATC 5 cut(s) 3, 166, 381, 513, 616
BssNI GRCGYC 1 cut(s) 162
Bst4CI ACNGT 1 cut(s) 296
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 113, 265
BstF5I GGATG 2 cut(s) 322, 662
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 3 cut(s) 117, 128, 335
BstKTI GATC 5 cut(s) 6, 169, 384, 516, 619
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 5 cut(s) 3, 166, 381, 513, 616
BstMWI GCNNNNNNNGC 3 cut(s) 123, 264, 644
BstNSI RCATGY 2 cut(s) 135, 267
BstSCI CCNGG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 77, 317
BsuRI GGCC 2 cut(s) 111, 232
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 322, 662
BtsI GCAGTG 2 cut(s) 37, 154
BtsIMutI CAGTG 2 cut(s) 37, 154
BveI ACCTGC 1 cut(s) 387
Cac8I GCNNGC 2 cut(s) 113, 265
CfoI GCGC 3 cut(s) 117, 128, 335
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 151
Csp6I GTAC 1 cut(s) 292
CspAI ACCGGT 1 cut(s) 533
CviAII CATG 5 cut(s) 29, 132, 264, 359, 655
CviQI GTAC 1 cut(s) 292
DpnI GATC 5 cut(s) 5, 168, 383, 515, 618
DpnII GATC 5 cut(s) 3, 166, 381, 513, 616
Ecl136II GAGCTC 2 cut(s) 352, 471
Eco24I GRGCYC 2 cut(s) 354, 473
Eco31I GGTCTC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 600
Eco47III AGCGCT 1 cut(s) 116
Eco53kI GAGCTC 2 cut(s) 352, 471
Eco57I CTGAAG 2 cut(s) 395, 608
Eco88I CYCGRG 2 cut(s) 56, 353
EcoICRI GAGCTC 2 cut(s) 352, 471
EcoRV GATATC 1 cut(s) 600
EcoT22I ATGCAT 1 cut(s) 265
EcoT38I GRGCYC 2 cut(s) 354, 473
FaeI CATG 5 cut(s) 32, 135, 267, 362, 658
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 5 cut(s) 28, 131, 263, 358, 654
FauI CCCGC 1 cut(s) 198
FauNDI CATATG 1 cut(s) 445
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 5 cut(s) 91, 129, 160, 233, 331
FokI GGATG 2 cut(s) 309, 649
FriOI GRGCYC 2 cut(s) 354, 473
Fsp4HI GCNGC 5 cut(s) 91, 129, 160, 233, 331
FspI TGCGCA 1 cut(s) 334
GlaI GCGC 3 cut(s) 116, 127, 334
GluI GCNGC 5 cut(s) 91, 129, 160, 233, 331
GsaI CCCAGC 1 cut(s) 614
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 2 cut(s) 111, 232
HapII CCGG 2 cut(s) 282, 534
HgaI GACGC 1 cut(s) 151
HhaI GCGC 3 cut(s) 117, 128, 335
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 5 cut(s) 32, 135, 267, 362, 658
Hin6I GCGC 3 cut(s) 115, 126, 333
HinP1I GCGC 3 cut(s) 115, 126, 333
HinfI GANTC 1 cut(s) 16
HpaII CCGG 2 cut(s) 282, 534
HphI GGTGA 2 cut(s) 565, 626
Hpy188I TCNGA 5 cut(s) 493, 510, 531, 588, 621
Hpy188III TCNNGA 4 cut(s) 385, 414, 517, 666
Hpy99I CGWCG 2 cut(s) 167, 625
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 5 cut(s) 93, 207, 263, 267, 420
HpyF10VI GCNNNNNNNGC 3 cut(s) 123, 264, 644
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 5 cut(s) 32, 135, 267, 362, 658
HspAI GCGC 3 cut(s) 115, 126, 333
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 5 cut(s) 3, 166, 381, 513, 616
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 9 cut(s) 59, 64, 125, 288, 295, 340, 382, 547, 596
Lsp1109I GCAGC 2 cut(s) 77, 317
LweI GCATC 3 cut(s) 186, 250, 407
MaeIII GTNAC 3 cut(s) 134, 277, 536
MalI GATC 5 cut(s) 5, 168, 383, 515, 618
MboI GATC 5 cut(s) 3, 166, 381, 513, 616
MboII GAAGA 3 cut(s) 250, 523, 601
MhlI GDGCHC 2 cut(s) 354, 473
MluCI AATT 2 cut(s) 68, 633
MmeI TCCRAC 2 cut(s) 414, 644
Mph1103I ATGCAT 1 cut(s) 265
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 2 cut(s) 282, 534
MspR9I CCNGG 1 cut(s) 283
MwoI GCNNNNNNNGC 3 cut(s) 123, 264, 644
NciI CCSGG 1 cut(s) 283
NdeI CATATG 1 cut(s) 445
NdeII GATC 5 cut(s) 3, 166, 381, 513, 616
NlaIII CATG 5 cut(s) 32, 135, 267, 362, 658
NmuCI GTSAC 1 cut(s) 536
NsbI TGCGCA 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 265
NspI RCATGY 2 cut(s) 135, 267
PaeI GCATGC 1 cut(s) 267
PaeR7I CTCGAG 2 cut(s) 56, 353
PaqCI CACCTGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 16
PinAI ACCGGT 1 cut(s) 533
PkrI GCNGC 5 cut(s) 92, 130, 161, 234, 332
Psp124BI GAGCTC 2 cut(s) 354, 473
PspFI CCCAGC 1 cut(s) 610
PspPI GGNCC 1 cut(s) 109
PspXI VCTCGAGB 2 cut(s) 56, 353
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SacI GAGCTC 2 cut(s) 354, 473
SatI GCNGC 5 cut(s) 91, 129, 160, 233, 331
Sau3AI GATC 5 cut(s) 3, 166, 381, 513, 616
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 2 cut(s) 354, 473
SfaNI GCATC 3 cut(s) 186, 250, 407
Sfr274I CTCGAG 2 cut(s) 56, 353
SlaI CTCGAG 2 cut(s) 56, 353
SmlI CTYRAG 4 cut(s) 56, 226, 353, 368
SmoI CTYRAG 4 cut(s) 56, 226, 353, 368
SphI GCATGC 1 cut(s) 267
Sse9I AATT 2 cut(s) 68, 633
SsiI CCGC 5 cut(s) 129, 159, 191, 233, 638
SspI AATATT 1 cut(s) 254
SstI GAGCTC 2 cut(s) 354, 473
StyD4I CCNGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 296
TaqI TCGA 9 cut(s) 57, 141, 165, 315, 354, 384, 413, 462, 473
TasI AATT 2 cut(s) 68, 633
TauI GCSGC 3 cut(s) 131, 162, 235
TfiI GAWTC 1 cut(s) 16
TscAI CASTG 2 cut(s) 44, 161
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 2 cut(s) 90, 330
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 2 cut(s) 45, 643
TspRI CASTG 2 cut(s) 44, 161
XapI RAATTY 1 cut(s) 68
XceI RCATGY 2 cut(s) 135, 267
XhoI CTCGAG 2 cut(s) 56, 353
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.