RLG00000014457

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0020290
Physical Location & Seq
Reverse (-)
53401781 .. 53403324
1544 bp
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UTR
Exon/CDS
Intron
RLM00000014457

Sequence Viewer

Length: 522 bp
ATGATCATTGACGGGAATCACATATCCCATGAAAGCCACCGCCACCAGCTCAAACTTGAGGACACAAAGTTCCCGTTCAGGTGTGATGGCGGCAAGGTGATAGGAATAGGCAAACGCTACACTTGCACCGCATGTAACTTCGACCTCCACAAGCATTGCGGAGTCGATCCTGATACTCCTATGTTCCACCCGCTGATGCCCAAGTGCAAAAACTTGAAGTTCTGCTCGAGGCCGCCGAAGAACGCCACTATTGCGATGCATGAACCAAGCCTGTGCGATTTCGACCTGCATCCCTGCTGCGCAAACTTTCCTATGGAGCTCGAGGATGGGAGCGTCAAGCTGAAGCTGATCGAGAAAGACCGCAGGTGGATTTATAGGTCGAGATGCAAGAAGTATAAGTTGGAAGTAGCATATGCGAGGGAAATAATCGAGGAGCGCTCGACTAAACTTTTGGGGTCAGAGATGACAAGCGTTCCGAAGATCAAGAATATGGTTCAGACAGTTACAGCAGAGGTAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

174

Amino Acids

19.96

Weight (kDa)

8.9

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 12 - 53 6.6e-07 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020290)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07740
rosa_chinensis RchiOBHm_Chr6g0262071 RchiOBHm_Chr6g0271941
rosa_laevigata RLG00000014457
rosa_samantha Rh6CG106200 Rh6DG096600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 354
Acc16I TGCGCA 1 cut(s) 301
Acc36I ACCTGC 2 cut(s) 294, 354
AciI CCGC 7 cut(s) 40, 90, 129, 159, 191, 233, 361
AclWI GGATC 1 cut(s) 161
AcuI CTGAAG 1 cut(s) 362
AfeI AGCGCT 1 cut(s) 437
AgsI TTSAA 1 cut(s) 217
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AluBI AGCT 4 cut(s) 49, 319, 340, 346
AluI AGCT 4 cut(s) 49, 319, 340, 346
Alw21I GWGCWC 1 cut(s) 321
AlwI GGATC 1 cut(s) 161
Ama87I CYCGRG 2 cut(s) 226, 320
Aor51HI AGCGCT 1 cut(s) 437
AoxI GGCC 1 cut(s) 230
ApeKI GCWGC 1 cut(s) 297
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
AspLEI GCGC 2 cut(s) 302, 438
AsuHPI GGTGA 1 cut(s) 109
AvaI CYCGRG 2 cut(s) 226, 320
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 284
BccI CCATC 2 cut(s) 80, 320
BclI TGATCA 1 cut(s) 3
BfoI RGCGCY 1 cut(s) 439
BfuAI ACCTGC 2 cut(s) 294, 354
BisI GCNGC 3 cut(s) 91, 233, 298
BlsI GCNGC 3 cut(s) 92, 234, 299
BmeT110I CYCGRG 2 cut(s) 226, 320
BmsI GCATC 4 cut(s) 186, 246, 298, 374
BplI GAGNNNNNCTC 2 cut(s) 422, 454
BpuEI CTTGAG 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 154
BseGI GGATG 2 cut(s) 289, 331
BseMI GCAATG 1 cut(s) 154
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 1 cut(s) 284
BshFI GGCC 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 2 cut(s) 226, 320
BsnI GGCC 1 cut(s) 232
BsoBI CYCGRG 2 cut(s) 226, 320
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 4 cut(s) 3, 166, 348, 480
BspACI CCGC 7 cut(s) 40, 90, 129, 159, 191, 233, 361
BspANI GGCC 1 cut(s) 232
BspMI ACCTGC 2 cut(s) 294, 354
BspPI GGATC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 154
BssMI GATC 4 cut(s) 3, 166, 348, 480
Bst4CI ACNGT 1 cut(s) 502
BstF5I GGATG 2 cut(s) 289, 331
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 2 cut(s) 302, 438
BstKTI GATC 4 cut(s) 6, 169, 351, 483
BstMBI GATC 4 cut(s) 3, 166, 348, 480
BstMWI GCNNNNNNNGC 2 cut(s) 123, 251
BstNSI RCATGY 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 284
BsuRI GGCC 1 cut(s) 232
BtgZI GCGATG 1 cut(s) 269
BtsCI GGATG 2 cut(s) 289, 331
BveI ACCTGC 2 cut(s) 294, 354
CfoI GCGC 2 cut(s) 302, 438
CseI GACGC 1 cut(s) 322
CspCI CAANNNNNGTGG 2 cut(s) 137, 172
CviAII CATG 3 cut(s) 29, 132, 260
CviJI RGCY 7 cut(s) 36, 49, 232, 270, 319, 340, 346
CviKI_1 RGCY 7 cut(s) 36, 49, 232, 270, 319, 340, 346
DpnI GATC 4 cut(s) 5, 168, 350, 482
DpnII GATC 4 cut(s) 3, 166, 348, 480
Ecl136II GAGCTC 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 321
Eco47III AGCGCT 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 362
Eco88I CYCGRG 2 cut(s) 226, 320
EcoICRI GAGCTC 1 cut(s) 319
EcoT22I ATGCAT 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 321
FaeI CATG 3 cut(s) 32, 135, 263
FatI CATG 3 cut(s) 28, 131, 259
FauI CCCGC 1 cut(s) 198
FauNDI CATATG 1 cut(s) 412
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 91, 233, 298
FokI GGATG 2 cut(s) 276, 338
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 3 cut(s) 91, 233, 298
FspI TGCGCA 1 cut(s) 301
GlaI GCGC 2 cut(s) 301, 437
GluI GCNGC 3 cut(s) 91, 233, 298
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 1 cut(s) 232
HgaI GACGC 1 cut(s) 322
HhaI GCGC 2 cut(s) 302, 438
Hin1II CATG 3 cut(s) 32, 135, 263
Hin6I GCGC 2 cut(s) 300, 436
HinP1I GCGC 2 cut(s) 300, 436
HinfI GANTC 2 cut(s) 16, 162
HphI GGTGA 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 460, 477, 498
Hpy188III TCNNGA 4 cut(s) 170, 352, 381, 484
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4V TGCA 5 cut(s) 126, 207, 259, 289, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 251
Hsp92II CATG 3 cut(s) 32, 135, 263
HspAI GCGC 2 cut(s) 300, 436
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 166, 348, 480
LmnI GCTCC 3 cut(s) 316, 330, 433
LpnPI CCDG 7 cut(s) 59, 64, 183, 284, 299, 307, 349
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 4 cut(s) 186, 246, 298, 374
MaeIII GTNAC 2 cut(s) 134, 502
MalI GATC 4 cut(s) 5, 168, 350, 482
MboI GATC 4 cut(s) 3, 166, 348, 480
MboII GAAGA 2 cut(s) 250, 490
MhlI GDGCHC 1 cut(s) 321
MlyI GAGTC 1 cut(s) 171
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 7 cut(s) 52, 155, 222, 316, 411, 424, 505
Mph1103I ATGCAT 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 193
MwoI GCNNNNNNNGC 2 cut(s) 123, 251
NdeI CATATG 1 cut(s) 412
NdeII GATC 4 cut(s) 3, 166, 348, 480
NlaIII CATG 3 cut(s) 32, 135, 263
NsbI TGCGCA 1 cut(s) 301
NsiI ATGCAT 1 cut(s) 261
NspI RCATGY 1 cut(s) 135
PaeR7I CTCGAG 2 cut(s) 226, 320
PaqCI CACCTGC 1 cut(s) 354
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PfeI GAWTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 92, 234, 299
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
Psp124BI GAGCTC 1 cut(s) 321
PspXI VCTCGAGB 2 cut(s) 226, 320
SacI GAGCTC 1 cut(s) 321
SatI GCNGC 3 cut(s) 91, 233, 298
Sau3AI GATC 4 cut(s) 3, 166, 348, 480
SchI GAGTC 1 cut(s) 171
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 4 cut(s) 186, 246, 298, 374
Sfr274I CTCGAG 2 cut(s) 226, 320
SlaI CTCGAG 2 cut(s) 226, 320
SmlI CTYRAG 3 cut(s) 56, 226, 320
SmoI CTYRAG 3 cut(s) 56, 226, 320
SsiI CCGC 7 cut(s) 40, 90, 129, 159, 191, 233, 361
SstI GAGCTC 1 cut(s) 321
TaaI ACNGT 1 cut(s) 502
TaqI TCGA 9 cut(s) 141, 165, 227, 282, 321, 351, 380, 429, 440
TauI GCSGC 2 cut(s) 93, 235
TfiI GAWTC 1 cut(s) 16
TseI GCWGC 1 cut(s) 297
TspDTI ATGAA 2 cut(s) 45, 276
XceI RCATGY 1 cut(s) 135
XhoI CTCGAG 2 cut(s) 226, 320
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.