RchiOBHm_Chr6g0293501

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0025076
Physical Location & Seq
Reverse (-)
56172540 .. 56176856
4317 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26335

Sequence Viewer

Length: 609 bp
ATGACACTGCCTTTGTGTCTTACTAAATGGTCTAATTCTGTTTCTTTGCTAAAACAAATTAATGACTTCCTTTTCATATGTGTAAAAGATAGAGGGAAGGGAAATAAATATGAAACTAGATGCCCTTATATTTATATTACAATTTCCAGTTTATTTTCGAAAATCATGATATCAACTGATTTGAACTTGGGAATTGTTGTGACATGTTTTCGTGCTCTTGCCAACATGCTCTATCTGCTTCATATTTTCATCAAGTTGAGGACAACTTATATATCACCAAAAACTAGAGTTTTTGGGAGGGGTGAACTTGTTATGGATCCCAAAAAGATTGCTAGAAGGTATCTGAGATCAGATTTCTTCGTAGATCTTATAGCTACACTGCCTCTCCCTCAGATTGTCATATGGTTCATCATACCTGCAGTTAAAAGCTCCAGTTCTGGCCATACCCATAATGCCCCTGTACTGATTGTTTTGCTCCAATATGTTCCTAGATTGTATCTCATTTTCCCACTAAGTTCTCAGATCATCAAAGCCACTGGTGTGGTCACAAAGACAGCTTGGGCCGGGGCTGCGTATAATCTCATACTCTACAGTCTACATGTTAGCTAG

Protein Analysis

202

Amino Acids

22.9

Weight (kDa)

9.91

Isoelectric Point (pI)

29.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 45 - 195 2.6e-06 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0025076)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0293501
rosa_samantha Rh5CG425800
rosa_wichuraiana Rw6G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 595
AclWI GGATC 2 cut(s) 311, 324
AcoI YGGCCR 1 cut(s) 439
AfaI GTAC 1 cut(s) 462
AflIII ACRYGT 2 cut(s) 203, 598
AgsI TTSAA 1 cut(s) 184
AleI CACNNNNGTG 1 cut(s) 539
AluBI AGCT 4 cut(s) 374, 429, 557, 606
AluI AGCT 4 cut(s) 374, 429, 557, 606
Alw21I GWGCWC 1 cut(s) 217
AlwI GGATC 2 cut(s) 311, 324
AoxI GGCC 2 cut(s) 439, 561
ApeKI GCWGC 1 cut(s) 569
ArsI GACNNNNNNTTYG 1 cut(s) 27
AseI ATTAAT 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 561
AsuC2I CCSGG 1 cut(s) 565
AsuHPI GGTGA 2 cut(s) 267, 314
AsuII TTCGAA 1 cut(s) 158
BalI TGGCCA 1 cut(s) 441
BamHI GGATCC 1 cut(s) 316
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 556
BcnI CCSGG 1 cut(s) 565
BfaI CTAG 5 cut(s) 117, 285, 333, 489, 607
BfmI CTRYAG 2 cut(s) 417, 589
BfuAI ACCTGC 1 cut(s) 424
BglII AGATCT 1 cut(s) 364
BisI GCNGC 1 cut(s) 570
BlsI GCNGC 1 cut(s) 571
Bme1390I CCNGG 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 561
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 565
BmsI GCATC 1 cut(s) 110
BpmI CTGGAG 1 cut(s) 415
Bpu14I TTCGAA 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 565
BsaJI CCNNGG 1 cut(s) 564
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bse1I ACTGG 3 cut(s) 147, 432, 541
BseDI CCNNGG 1 cut(s) 564
BseMII CTCAG 3 cut(s) 335, 404, 533
BseNI ACTGG 3 cut(s) 147, 432, 541
BseXI GCAGC 1 cut(s) 556
BshFI GGCC 2 cut(s) 441, 563
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 564
BsnI GGCC 2 cut(s) 441, 563
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 4 cut(s) 316, 347, 364, 522
BspANI GGCC 2 cut(s) 441, 563
BspCNI CTCAG 3 cut(s) 336, 403, 532
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 318
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 424
BspPI GGATC 2 cut(s) 311, 324
BspT104I TTCGAA 1 cut(s) 158
BsrI ACTGG 3 cut(s) 147, 432, 541
BssECI CCNNGG 1 cut(s) 564
BssMI GATC 4 cut(s) 316, 347, 364, 522
Bst4CI ACNGT 1 cut(s) 593
BstBI TTCGAA 1 cut(s) 158
BstDEI CTNAG 4 cut(s) 344, 390, 512, 519
BstKTI GATC 4 cut(s) 319, 350, 367, 525
BstMBI GATC 4 cut(s) 316, 347, 364, 522
BstMWI GCNNNNNNNGC 2 cut(s) 235, 569
BstNSI RCATGY 3 cut(s) 207, 229, 602
BstSCI CCNGG 1 cut(s) 563
BstSFI CTRYAG 2 cut(s) 417, 589
BstV1I GCAGC 1 cut(s) 556
BstX2I RGATCY 2 cut(s) 316, 364
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 2 cut(s) 316, 364
BsuRI GGCC 2 cut(s) 441, 563
BtsI GCAGTG 2 cut(s) 5, 377
BtsIMutI CAGTG 3 cut(s) 5, 377, 534
BveI ACCTGC 1 cut(s) 424
CciI TCATGA 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 561
Csp6I GTAC 1 cut(s) 461
CviAII CATG 4 cut(s) 166, 204, 226, 599
CviJI RGCY 8 cut(s) 374, 429, 441, 533, 557, 563, 569, 606
CviKI_1 RGCY 8 cut(s) 374, 429, 441, 533, 557, 563, 569, 606
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 4 cut(s) 344, 390, 512, 519
DpnI GATC 4 cut(s) 318, 349, 366, 524
DpnII GATC 4 cut(s) 316, 347, 364, 522
EaeI YGGCCR 1 cut(s) 439
Eco32I GATATC 1 cut(s) 171
EcoRV GATATC 1 cut(s) 171
FaeI CATG 4 cut(s) 169, 207, 229, 602
FatI CATG 4 cut(s) 165, 203, 225, 598
FauNDI CATATG 2 cut(s) 77, 401
FblI GTMKAC 1 cut(s) 595
Fnu4HI GCNGC 1 cut(s) 570
Fsp4HI GCNGC 1 cut(s) 570
FspBI CTAG 5 cut(s) 117, 285, 333, 489, 607
GluI GCNGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 2 cut(s) 441, 563
HapII CCGG 1 cut(s) 564
Hin1II CATG 4 cut(s) 169, 207, 229, 602
HpaII CCGG 1 cut(s) 564
HphI GGTGA 2 cut(s) 267, 314
Hpy166II GTNNAC 2 cut(s) 305, 596
Hpy188I TCNGA 4 cut(s) 345, 352, 393, 522
Hpy188III TCNNGA 1 cut(s) 166
Hpy8I GTNNAC 2 cut(s) 305, 596
HpyAV CCTTC 2 cut(s) 91, 330
HpyCH4III ACNGT 1 cut(s) 593
HpyCH4V TGCA 1 cut(s) 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 569
HpyF3I CTNAG 4 cut(s) 344, 390, 512, 519
Hsp92II CATG 4 cut(s) 169, 207, 229, 602
Kzo9I GATC 4 cut(s) 316, 347, 364, 522
LmnI GCTCC 2 cut(s) 434, 480
LpnPI CCDG 7 cut(s) 160, 423, 429, 445, 471, 522, 577
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 1 cut(s) 110
MaeI CTAG 5 cut(s) 117, 285, 333, 489, 607
MaeIII GTNAC 2 cut(s) 199, 544
MalI GATC 4 cut(s) 318, 349, 366, 524
MboI GATC 4 cut(s) 316, 347, 364, 522
MboII GAAGA 1 cut(s) 349
MflI RGATCY 2 cut(s) 316, 364
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 441
MluCI AATT 4 cut(s) 34, 57, 141, 192
MluNI TGGCCA 1 cut(s) 441
MnlI CCTC 5 cut(s) 86, 252, 291, 393, 399
Mox20I TGGCCA 1 cut(s) 441
MscI TGGCCA 1 cut(s) 441
MseI TTAA 2 cut(s) 60, 423
MslI CAYNNNNRTG 1 cut(s) 539
Msp20I TGGCCA 1 cut(s) 441
MspI CCGG 1 cut(s) 564
MspR9I CCNGG 1 cut(s) 565
MwoI GCNNNNNNNGC 2 cut(s) 235, 569
NciI CCSGG 1 cut(s) 565
NdeI CATATG 2 cut(s) 77, 401
NdeII GATC 4 cut(s) 316, 347, 364, 522
NlaIII CATG 4 cut(s) 169, 207, 229, 602
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 199, 544
NspI RCATGY 3 cut(s) 207, 229, 602
NspV TTCGAA 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 539
PagI TCATGA 1 cut(s) 165
PciI ACATGT 2 cut(s) 203, 598
PkrI GCNGC 1 cut(s) 571
PscI ACATGT 2 cut(s) 203, 598
PshBI ATTAAT 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 561
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 2 cut(s) 316, 364
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 2 cut(s) 60, 423
SatI GCNGC 1 cut(s) 570
Sau3AI GATC 4 cut(s) 316, 347, 364, 522
Sau96I GGNCC 1 cut(s) 561
ScrFI CCNGG 1 cut(s) 565
SduI GDGCHC 1 cut(s) 217
SetI ASST 6 cut(s) 341, 376, 418, 431, 559, 608
SfaNI GCATC 1 cut(s) 110
SfcI CTRYAG 2 cut(s) 417, 589
SfuI TTCGAA 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 539
Sse9I AATT 4 cut(s) 34, 57, 141, 192
SspMI CTAG 5 cut(s) 117, 285, 333, 489, 607
StyD4I CCNGG 1 cut(s) 563
TaaI ACNGT 1 cut(s) 593
TaqI TCGA 1 cut(s) 158
TasI AATT 4 cut(s) 34, 57, 141, 192
TatI WGTACW 1 cut(s) 460
Tru1I TTAA 2 cut(s) 60, 423
Tru9I TTAA 2 cut(s) 60, 423
TscAI CASTG 3 cut(s) 12, 384, 541
TseFI GTSAC 2 cut(s) 199, 544
TseI GCWGC 1 cut(s) 569
Tsp45I GTSAC 2 cut(s) 199, 544
TspDTI ATGAA 5 cut(s) 64, 126, 230, 238, 397
TspRI CASTG 3 cut(s) 12, 384, 541
VspI ATTAAT 1 cut(s) 60
XceI RCATGY 3 cut(s) 207, 229, 602
XmiI GTMKAC 1 cut(s) 595
XspI CTAG 5 cut(s) 117, 285, 333, 489, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.