RchiOBHm_Chr7g0182441

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0022703
Physical Location & Seq
Forward (+)
3685821 .. 3691540
5720 bp
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UTR
Exon/CDS
Intron
PRQ16275

Sequence Viewer

Length: 615 bp
ATGCAAGTAAAAGGTGCGCAGCTGATATTTGATCTTACCGCAAAGAAACTTATCAGTTATGACTCAGAAAAGTGCTCGGAGAAGAATTTACGCAAAAACTTCGTTGCATTCATACAAGGATTGATTTCCTTCCTTGTGGACATCCCTGGAACAGCTTATCACAAATGTTTACAGGGTAGGAAAAATGCAATGAGAATGTTGGTAAACCTGCTACAGGAAAGACGAGAAAAGCCGCGAAAGCAGCCTATAGATTTTTTTGATCACGTGCTTGAAGAACTCAAGAAAGAGGAAACGATGTTAACAGAAGAAATTTTTTTGGGTTTAATGTTTGTACTGCTTTTCGCGAGCTTTGAAACAACTTCCCTAGCTAGTTCAGACCATCCTGCATTGCTAAAGAAATTAACGGAAGAGCATGGGATGATCATAAAGCAAAGGGAAAATGCTGATGCTGGACTTACATGGAAAGAATACAAATCAATGACATTCACATTTCAGATCATCAACGAAACAGTTAGACTGGCAAATATAGTTCCAGCTATCTTTCGCAAAGCAATAAGAGAGATACAGTTTAAAGGTATGCAAATCATAATATTTAGTGTGGGATTCCAACTATAA

Protein Analysis

204

Amino Acids

23.61

Weight (kDa)

9.17

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 195 1.6e-17 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022703)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0469691 RchiOBHm_Chr7g0182441
rosa_samantha Rh3BG177100 Rh7DG049900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 216
AccII CGCG 2 cut(s) 235, 344
AciI CCGC 2 cut(s) 39, 233
AcsI RAATTY 2 cut(s) 85, 309
AcvI CACGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 333
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 2 cut(s) 272, 353
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 5 cut(s) 22, 155, 348, 368, 536
AluI AGCT 5 cut(s) 22, 155, 348, 368, 536
Alw21I GWGCWC 1 cut(s) 77
ApeKI GCWGC 2 cut(s) 19, 241
ApoI RAATTY 2 cut(s) 85, 309
AspLEI GCGC 1 cut(s) 19
BbrPI CACGTG 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 2 cut(s) 31, 253
BccI CCATC 1 cut(s) 387
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 2 cut(s) 259, 420
BfaI CTAG 2 cut(s) 365, 369
BfmI CTRYAG 2 cut(s) 212, 246
BfuAI ACCTGC 1 cut(s) 216
BisI GCNGC 3 cut(s) 20, 233, 242
BlsI GCNGC 3 cut(s) 21, 234, 243
Bme1390I CCNGG 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 436
BpuEI CTTGAG 1 cut(s) 263
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 522
Bse3DI GCAATG 2 cut(s) 195, 386
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 3 cut(s) 141, 379, 423
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 2 cut(s) 195, 386
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 522
BseXI GCAGC 2 cut(s) 31, 253
Bsh1236I CGCG 2 cut(s) 235, 344
BsiHKAI GWGCWC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 214
BsmI GAATGC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 4 cut(s) 31, 259, 420, 495
Bsp68I TCGCGA 1 cut(s) 344
BspACI CCGC 2 cut(s) 39, 233
BspCNI CTCAG 1 cut(s) 77
BspFNI CGCG 2 cut(s) 235, 344
BspMI ACCTGC 1 cut(s) 216
BspQI GCTCTTC 1 cut(s) 402
BsrDI GCAATG 2 cut(s) 195, 386
BsrI ACTGG 1 cut(s) 522
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 4 cut(s) 31, 259, 420, 495
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 511, 567
Bst6I CTCTTC 1 cut(s) 402
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 1 cut(s) 64
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 3 cut(s) 141, 379, 423
BstFNI CGCG 2 cut(s) 235, 344
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 4 cut(s) 34, 262, 423, 498
BstMBI GATC 4 cut(s) 31, 259, 420, 495
BstMWI GCNNNNNNNGC 2 cut(s) 238, 241
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 2 cut(s) 212, 246
BstUI CGCG 2 cut(s) 235, 344
BstV1I GCAGC 2 cut(s) 31, 253
BtsCI GGATG 3 cut(s) 141, 379, 423
BtuMI TCGCGA 1 cut(s) 344
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 346
CfoI GCGC 1 cut(s) 19
Csp6I GTAC 1 cut(s) 332
CviAII CATG 2 cut(s) 413, 459
CviJI RGCY 7 cut(s) 22, 155, 232, 244, 348, 368, 536
CviKI_1 RGCY 7 cut(s) 22, 155, 232, 244, 348, 368, 536
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 1 cut(s) 64
DpnI GATC 4 cut(s) 33, 261, 422, 497
DpnII GATC 4 cut(s) 31, 259, 420, 495
DraI TTTAAA 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco72I CACGTG 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 2 cut(s) 416, 462
FatI CATG 2 cut(s) 412, 458
FbaI TGATCA 2 cut(s) 259, 420
Fnu4HI GCNGC 3 cut(s) 20, 233, 242
FokI GGATG 3 cut(s) 128, 366, 430
Fsp4HI GCNGC 3 cut(s) 20, 233, 242
FspBI CTAG 2 cut(s) 365, 369
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 1 cut(s) 18
GluI GCNGC 3 cut(s) 20, 233, 242
HhaI GCGC 1 cut(s) 19
Hin1II CATG 2 cut(s) 416, 462
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 2 cut(s) 62, 603
HpaI GTTAAC 1 cut(s) 300
Hpy166II GTNNAC 4 cut(s) 139, 170, 205, 300
Hpy188I TCNGA 4 cut(s) 67, 79, 376, 495
Hpy188III TCNNGA 2 cut(s) 280, 343
Hpy8I GTNNAC 4 cut(s) 139, 170, 205, 300
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 2 cut(s) 511, 567
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 4, 107, 188, 386, 580
HpyF10VI GCNNNNNNNGC 2 cut(s) 238, 241
HpyF3I CTNAG 1 cut(s) 64
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 416, 462
HspAI GCGC 1 cut(s) 17
Ksp22I TGATCA 2 cut(s) 259, 420
KspAI GTTAAC 1 cut(s) 300
Kzo9I GATC 4 cut(s) 31, 259, 420, 495
LguI GCTCTTC 1 cut(s) 402
LpnPI CCDG 9 cut(s) 132, 158, 159, 200, 221, 396, 435, 503, 546
Lsp1109I GCAGC 2 cut(s) 31, 253
LweI GCATC 1 cut(s) 436
MaeI CTAG 2 cut(s) 365, 369
MaeII ACGT 1 cut(s) 264
MalI GATC 4 cut(s) 33, 261, 422, 497
MboI GATC 4 cut(s) 31, 259, 420, 495
MboII GAAGA 4 cut(s) 94, 284, 317, 419
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 3 cut(s) 85, 309, 398
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 1 cut(s) 280
MseI TTAA 4 cut(s) 299, 323, 401, 570
MspA1I CMGCKG 1 cut(s) 22
MspR9I CCNGG 1 cut(s) 147
Mva1269I GAATGC 1 cut(s) 107
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 2 cut(s) 235, 344
MwoI GCNNNNNNNGC 2 cut(s) 238, 241
NdeII GATC 4 cut(s) 31, 259, 420, 495
NlaIII CATG 2 cut(s) 416, 462
NruI TCGCGA 1 cut(s) 344
NsbI TGCGCA 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 402
PctI GAATGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 603
PkrI GCNGC 3 cut(s) 21, 234, 243
PleI GAGTC 1 cut(s) 56
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 1 cut(s) 56
Ppu21I YACGTR 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 145
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 22
RruI TCGCGA 1 cut(s) 344
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 402
SaqAI TTAA 4 cut(s) 299, 323, 401, 570
SatI GCNGC 3 cut(s) 20, 233, 242
Sau3AI GATC 4 cut(s) 31, 259, 420, 495
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 77
SetI ASST 9 cut(s) 16, 24, 157, 210, 267, 350, 370, 538, 577
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 2 cut(s) 212, 246
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 3 cut(s) 85, 309, 398
SsiI CCGC 2 cut(s) 39, 233
SspI AATATT 1 cut(s) 591
SspMI CTAG 2 cut(s) 365, 369
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 2 cut(s) 511, 567
TaiI ACGT 1 cut(s) 267
TasI AATT 3 cut(s) 85, 309, 398
TatI WGTACW 1 cut(s) 331
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 1 cut(s) 603
Tru1I TTAA 4 cut(s) 299, 323, 401, 570
Tru9I TTAA 4 cut(s) 299, 323, 401, 570
TseI GCWGC 2 cut(s) 19, 241
TspDTI ATGAA 1 cut(s) 100
TspGWI ACGGA 1 cut(s) 419
XagI CCTNNNNNAGG 1 cut(s) 212
XapI RAATTY 2 cut(s) 85, 309
XspI CTAG 2 cut(s) 365, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.