RchiOBHm_Chr7g0213121

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0024800
Physical Location & Seq
Forward (+)
30279802 .. 30283595
3794 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19075

Sequence Viewer

Length: 780 bp
ATGACATACACGTCTTTTTTTTGCAGGAGATCGAGTTTCCTGCTACTGGACTTGACTCTGTTCCAGTTGGACTATCTGACTAAGCCATCGTTTTTCGAGGATTTTGGTTTTACTGAGAAAGTGAAAATGTCGGACCATGTGAAGCCAGAAGATGAACTATTCTTGGCTGCATATCCATATTTTAATGTCTCCGCGGGTAAGTCCAAGTATCCCTTCTTATCATTGTTAGGAAAAGTTGAATGCTGCAGAAACACATATTCAGAGTATGAAACGCTTGTAGTCGAAGAGCTGTACAGAGAAGCTGTTGTGAACACTGCCAGAAAGCTAATTATTTTCAACGGAGAACTTGACCGAATAAGATCTGGATGGCAGTTCAATACGTGGTTGTGCTTGGTCTTCTTGGTCTGCAATGTAGCAACAAGCTTTGTCTATTGTACTTGGGTGAGAGAGCACGAGTTGTCTTCCAATTGTCTTGTGCTGGAGTGTTTTGTTCTCTTGTACAGCCTTGTAATCTTGTGCTTTTCTTCTGGTAATGTCTTCATCTTATCACTGGGTGTATCCTTTCTCGCTGCACTTTTGAAGACCCTGTTTCCAAGTATGGAGACTGTGTACTACATTCACAATTTTAAAGGACGCTCTGGAGGGACCCTTTTCAGGTGCTACCCAGGTCCATGGAAAGTCTTCAGAAAAGTGAGGAATAAATACATTTGTTTGCATCAGCAGGAAGCTATGCCATCTCTCAAGGAAGTTGCCTTGGAAATTCTTCCAGGTAAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

259

Amino Acids

30.0

Weight (kDa)

8.23

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 89 - 251 3.8e-19 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024800)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0148561 RchiOBHm_Chr4g0386461 RchiOBHm_Chr7g0213121

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AccII CGCG 1 cut(s) 194
AciI CCGC 2 cut(s) 192, 194
AcsI RAATTY 1 cut(s) 759
AcuI CTGAAG 1 cut(s) 667
AdeI CACNNNGTG 1 cut(s) 554
AfaI GTAC 4 cut(s) 293, 436, 500, 611
AfiI CCNNNNNNNGG 2 cut(s) 46, 654
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 4 cut(s) 239, 337, 376, 580
AjiI CACGTC 1 cut(s) 12
AjnI CCWGG 2 cut(s) 664, 766
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 289, 302, 325, 423, 728
AluI AGCT 5 cut(s) 289, 302, 325, 423, 728
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 2 cut(s) 193, 596
ApeKI GCWGC 3 cut(s) 167, 243, 569
ApoI RAATTY 1 cut(s) 759
Asp700I GAANNNNTTC 2 cut(s) 680, 762
AspS9I GGNCC 3 cut(s) 133, 645, 668
AsuHPI GGTGA 1 cut(s) 454
AvaII GGWCC 3 cut(s) 133, 645, 668
BauI CACGAG 1 cut(s) 452
BbsI GAAGAC 5 cut(s) 388, 453, 529, 587, 673
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 154, 230, 556
BccI CCATC 3 cut(s) 94, 360, 742
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 2 cut(s) 666, 768
BciVI GTATCC 2 cut(s) 219, 568
BcoDI GTCTC 2 cut(s) 193, 596
BfaI CTAG 1 cut(s) 778
BfmI CTRYAG 1 cut(s) 244
BfuI GTATCC 2 cut(s) 219, 568
BglII AGATCT 1 cut(s) 359
BisI GCNGC 3 cut(s) 168, 244, 570
BlsI GCNGC 3 cut(s) 169, 245, 571
Bme1390I CCNGG 2 cut(s) 666, 768
Bme18I GGWCC 3 cut(s) 133, 645, 668
BmgBI CACGTC 1 cut(s) 12
BmgT120I GGNCC 3 cut(s) 133, 645, 668
BmiI GGNNCC 2 cut(s) 646, 647
BmrFI CCNGG 2 cut(s) 666, 768
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 1 cut(s) 724
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 5 cut(s) 388, 453, 529, 587, 673
BpmI CTGGAG 2 cut(s) 500, 660
BpuEI CTTGAG 1 cut(s) 725
BsaAI YACGTR 1 cut(s) 381
BsaJI CCNNGG 4 cut(s) 192, 664, 671, 753
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 593, 623
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 654
Bse1I ACTGG 3 cut(s) 51, 64, 555
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 2 cut(s) 666, 768
BseDI CCNNGG 4 cut(s) 192, 664, 671, 753
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 2 cut(s) 46, 654
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 3 cut(s) 51, 64, 555
BseXI GCAGC 3 cut(s) 154, 230, 556
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 453
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 2 cut(s) 46, 654
BsmAI GTCTC 2 cut(s) 193, 596
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 453
Bsp1407I TGTACA 2 cut(s) 291, 498
Bsp143I GATC 2 cut(s) 29, 359
Bsp19I CCATGG 1 cut(s) 671
BspACI CCGC 2 cut(s) 192, 194
BspCNI CTCAG 1 cut(s) 106
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 646, 647
BspMAI CTGCAG 1 cut(s) 248
BspQI GCTCTTC 1 cut(s) 279
BsrDI GCAATG 1 cut(s) 415
BsrGI TGTACA 2 cut(s) 291, 498
BsrI ACTGG 3 cut(s) 51, 64, 555
BssECI CCNNGG 4 cut(s) 192, 664, 671, 753
BssMI GATC 2 cut(s) 29, 359
BssSI CACGAG 1 cut(s) 452
BssT1I CCWWGG 2 cut(s) 671, 753
Bst2BI CACGAG 1 cut(s) 452
Bst2UI CCWGG 2 cut(s) 666, 768
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 1 cut(s) 279
BstAUI TGTACA 2 cut(s) 291, 498
BstBAI YACGTR 1 cut(s) 381
BstDEI CTNAG 2 cut(s) 81, 114
BstDSI CCRYGG 2 cut(s) 192, 671
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 194
BstKTI GATC 2 cut(s) 32, 362
BstMAI GTCTC 2 cut(s) 193, 596
BstMBI GATC 2 cut(s) 29, 359
BstNI CCWGG 2 cut(s) 666, 768
BstSCI CCNGG 2 cut(s) 664, 766
BstSFI CTRYAG 1 cut(s) 244
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 3 cut(s) 154, 230, 556
BstV2I GAAGAC 5 cut(s) 388, 453, 529, 587, 673
BstX2I RGATCY 1 cut(s) 359
BstXI CCANNNNNNTGG 1 cut(s) 672
BstYI RGATCY 1 cut(s) 359
BsuI GTATCC 2 cut(s) 219, 568
BtgI CCRYGG 2 cut(s) 192, 671
BtrI CACGTC 1 cut(s) 12
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 312, 548
Cfr13I GGNCC 3 cut(s) 133, 645, 668
Cfr42I CCGCGG 1 cut(s) 195
CseI GACGC 1 cut(s) 642
Csp6I GTAC 4 cut(s) 292, 435, 499, 610
CviAII CATG 2 cut(s) 137, 672
CviJI RGCY 9 cut(s) 85, 145, 167, 289, 302, 325, 423, 504, 728
CviKI_1 RGCY 9 cut(s) 85, 145, 167, 289, 302, 325, 423, 504, 728
CviQI GTAC 4 cut(s) 292, 435, 499, 610
DdeI CTNAG 2 cut(s) 81, 114
DpnI GATC 2 cut(s) 31, 361
DpnII GATC 2 cut(s) 29, 359
DraI TTTAAA 1 cut(s) 628
DraIII CACNNNGTG 1 cut(s) 554
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco130I CCWWGG 2 cut(s) 671, 753
Eco47I GGWCC 3 cut(s) 133, 645, 668
Eco57I CTGAAG 1 cut(s) 667
EcoO109I RGGNCCY 1 cut(s) 645
EcoRII CCWGG 2 cut(s) 664, 766
EcoT14I CCWWGG 2 cut(s) 671, 753
ErhI CCWWGG 2 cut(s) 671, 753
FaeI CATG 2 cut(s) 140, 675
FaiI YATR 9 cut(s) 7, 138, 172, 178, 256, 267, 599, 673, 731
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 1 cut(s) 658
FatI CATG 2 cut(s) 136, 671
FauI CCCGC 1 cut(s) 187
Fnu4HI GCNGC 3 cut(s) 168, 244, 570
FokI GGATG 1 cut(s) 378
Fsp4HI GCNGC 3 cut(s) 168, 244, 570
FspBI CTAG 1 cut(s) 778
GluI GCNGC 3 cut(s) 168, 244, 570
GsuI CTGGAG 2 cut(s) 500, 660
HgaI GACGC 1 cut(s) 642
Hin1II CATG 2 cut(s) 140, 675
HindIII AAGCTT 1 cut(s) 421
HinfI GANTC 1 cut(s) 55
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 3 cut(s) 310, 610, 773
Hpy188I TCNGA 4 cut(s) 78, 133, 262, 686
Hpy188III TCNNGA 2 cut(s) 363, 639
Hpy8I GTNNAC 3 cut(s) 310, 610, 773
HpyAV CCTTC 1 cut(s) 223
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 2 cut(s) 11, 380
HpyCH4V TGCA 6 cut(s) 24, 170, 246, 408, 572, 715
HpyF3I CTNAG 2 cut(s) 81, 114
HpySE526I ACGT 2 cut(s) 11, 380
Hsp92II CATG 2 cut(s) 140, 675
KflI GGGWCCC 1 cut(s) 645
KspI CCGCGG 1 cut(s) 195
Kzo9I GATC 2 cut(s) 29, 359
LguI GCTCTTC 1 cut(s) 279
Lsp1109I GCAGC 3 cut(s) 154, 230, 556
LweI GCATC 1 cut(s) 724
MaeI CTAG 1 cut(s) 778
MaeII ACGT 2 cut(s) 11, 380
MalI GATC 2 cut(s) 31, 361
MboI GATC 2 cut(s) 29, 359
MboII GAAGA 9 cut(s) 161, 296, 388, 453, 516, 529, 592, 673, 755
MfeI CAATTG 1 cut(s) 466
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 1 cut(s) 453
MluCI AATT 4 cut(s) 327, 466, 622, 759
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 2 cut(s) 48, 111
MnlI CCTC 3 cut(s) 91, 635, 687
MroXI GAANNNNTTC 2 cut(s) 680, 762
MseI TTAA 2 cut(s) 183, 627
MspA1I CMGCKG 1 cut(s) 194
MspR9I CCNGG 2 cut(s) 666, 768
MunI CAATTG 1 cut(s) 466
Mva1269I GAATGC 1 cut(s) 245
MvaI CCWGG 2 cut(s) 666, 768
MvnI CGCG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 671
NdeII GATC 2 cut(s) 29, 359
NlaIII CATG 2 cut(s) 140, 675
NlaIV GGNNCC 2 cut(s) 646, 647
PciSI GCTCTTC 1 cut(s) 279
PctI GAATGC 1 cut(s) 245
PdmI GAANNNNTTC 2 cut(s) 680, 762
PkrI GCNGC 3 cut(s) 169, 245, 571
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
Ppu21I YACGTR 1 cut(s) 381
PpuMI RGGWCCY 1 cut(s) 645
Psp5II RGGWCCY 1 cut(s) 645
Psp6I CCWGG 2 cut(s) 664, 766
PspGI CCWGG 2 cut(s) 664, 766
PspN4I GGNNCC 2 cut(s) 646, 647
PspPI GGNCC 3 cut(s) 133, 645, 668
PspPPI RGGWCCY 1 cut(s) 645
PstI CTGCAG 1 cut(s) 248
PsuI RGATCY 1 cut(s) 359
RsaI GTAC 4 cut(s) 293, 436, 500, 611
RsaNI GTAC 4 cut(s) 292, 435, 499, 610
SacII CCGCGG 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 2 cut(s) 183, 627
SatI GCNGC 3 cut(s) 168, 244, 570
Sau3AI GATC 2 cut(s) 29, 359
Sau96I GGNCC 3 cut(s) 133, 645, 668
SchI GAGTC 1 cut(s) 49
ScrFI CCNGG 2 cut(s) 666, 768
SduI GDGCHC 1 cut(s) 453
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 1 cut(s) 244
Sfr303I CCGCGG 1 cut(s) 195
SgrBI CCGCGG 1 cut(s) 195
SinI GGWCC 3 cut(s) 133, 645, 668
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 4 cut(s) 327, 466, 622, 759
SsiI CCGC 2 cut(s) 192, 194
SspMI CTAG 1 cut(s) 778
StyD4I CCNGG 2 cut(s) 664, 766
StyI CCWWGG 2 cut(s) 671, 753
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 2 cut(s) 14, 383
TaqI TCGA 3 cut(s) 32, 96, 282
TaqII GACCGA 1 cut(s) 366
TasI AATT 4 cut(s) 327, 466, 622, 759
TatI WGTACW 4 cut(s) 291, 434, 498, 609
Tru1I TTAA 2 cut(s) 183, 627
Tru9I TTAA 2 cut(s) 183, 627
TscAI CASTG 2 cut(s) 319, 555
TseI GCWGC 3 cut(s) 167, 243, 569
TspDTI ATGAA 3 cut(s) 168, 282, 529
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 2 cut(s) 319, 555
VpaK11BI GGWCC 3 cut(s) 133, 645, 668
XapI RAATTY 1 cut(s) 759
XmnI GAANNNNTTC 2 cut(s) 680, 762
XspI CTAG 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.