RchiOBHm_Chr7g0227341

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0022815
Physical Location & Seq
Forward (+)
50599999 .. 50600601
603 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20361

Sequence Viewer

Length: 585 bp
ATGTCTAATGGCATAATTCTAGTAGTGTTAGATGAAGCTGTTCACCTTTTTCAACAAATGACACAGGGACACTGTAAACCCAATGCCATCACTTGTAGTATATTGATAGATGCATTCTGTAAAGAAGGTAAAATGGACGATGCAGTGTTATTGTTTGATCAAATGCTGGAAAAGGATCCTGAACCTAATGTGGTCACCTATAGTTGTCTGATTGACGGTTATTTTAAATCTGAAAACATGGAAAGTGCCTTTGAACTATATGAAGAGATGCTTCAGAGTATCTCTCCAAATATAGTTAGTTACAGCATCCTCATTGATGGTCTTTGCAAAAGAGGACTGATGAAGAAAGCAGCCCTTGCATTTCATTCTGCTGTAAACAGGGGTCTGGTGCCTGATGTAGTAGCATATGGGATCCTGATTCATGGTTACTGCAAGGTTGGAAGAACGGCAGAAGCCATGGCATTGTACGGTCGTATGTTAATAAGTGGAATCATGCCGGATGCTATCATACAAAGGATGATTGCGAAGCACCATCTTCAAATTCTGTCCCACCAAAATATGAGCGCCCAACCAGATGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

194

Amino Acids

21.51

Weight (kDa)

5.58

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 10 - 41 1.8e-08 PPR repeat family
PPR_long PF17177 10 - 105 6.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 25 - 55 9.3e-12 PPR repeat
PPR_2 PF13041 30 - 76 3e-15 PPR repeat family
PPR PF01535 30 - 58 5.8e-08 PPR repeat
PPR_3 PF13812 51 - 106 7.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 61 - 90 1.6e-10 PPR repeat
PPR_2 PF13041 62 - 110 1.7e-18 PPR repeat family
PPR PF01535 65 - 92 1e-07 PPR repeat
PPR_1 PF12854 94 - 117 1.2e-08 PPR repeat
PPR_2 PF13041 96 - 145 1.1e-13 PPR repeat family
PPR_1 PF12854 127 - 159 4e-11 PPR repeat
PPR_2 PF13041 131 - 168 1.6e-09 PPR repeat family
PPR PF01535 134 - 164 9.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022815)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0227251 RchiOBHm_Chr7g0227341
rosa_samantha Rh7AG392300 Rh7CG411400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 388
AclWI GGATC 4 cut(s) 170, 183, 406, 419
AcsI RAATTY 1 cut(s) 540
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 1 cut(s) 467
AgsI TTSAA 3 cut(s) 53, 254, 539
AjuI GAANNNNNNNTTGG 1 cut(s) 561
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
AlwI GGATC 4 cut(s) 170, 183, 406, 419
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 1 cut(s) 540
Asp700I GAANNNNTTC 1 cut(s) 39
AspLEI GCGC 1 cut(s) 566
AsuHPI GGTGA 2 cut(s) 35, 187
BamHI GGATCC 2 cut(s) 175, 411
BanI GGYRCC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 362
BccI CCATC 3 cut(s) 95, 311, 540
BceAI ACGGC 1 cut(s) 462
BclI TGATCA 1 cut(s) 157
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 199
BfoI RGCGCY 1 cut(s) 567
BisI GCNGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 352
BmiI GGNNCC 3 cut(s) 177, 390, 413
BmsI GCATC 5 cut(s) 100, 130, 258, 315, 490
BplI GAGNNNNNCTC 2 cut(s) 268, 300
BsaJI CCNNGG 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 3 cut(s) 306, 505, 522
BseXI GCAGC 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 472
BshNI GGYRCC 1 cut(s) 388
BsiEI CGRYCG 1 cut(s) 472
BsiSI CCGG 1 cut(s) 497
BslFI GGGAC 2 cut(s) 81, 532
BsmFI GGGAC 2 cut(s) 81, 532
BsmI GAATGC 1 cut(s) 113
Bsp143I GATC 3 cut(s) 157, 175, 411
Bsp19I CCATGG 1 cut(s) 456
BspLI GGNNCC 3 cut(s) 177, 390, 413
BspPI GGATC 4 cut(s) 170, 183, 406, 419
BspT107I GGYRCC 1 cut(s) 388
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 3 cut(s) 157, 175, 411
BssT1I CCWWGG 1 cut(s) 456
Bst4CI ACNGT 3 cut(s) 74, 218, 470
Bst6I CTCTTC 1 cut(s) 258
BstAPI GCANNNNNTGC 1 cut(s) 356
BstDSI CCRYGG 1 cut(s) 456
BstEII GGTNACC 1 cut(s) 193
BstF5I GGATG 3 cut(s) 306, 505, 522
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 3 cut(s) 160, 178, 414
BstMBI GATC 3 cut(s) 157, 175, 411
BstMCI CGRYCG 1 cut(s) 472
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstPI GGTNACC 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 199
BstV1I GCAGC 1 cut(s) 362
BstX2I RGATCY 2 cut(s) 175, 411
BstYI RGATCY 2 cut(s) 175, 411
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 3 cut(s) 306, 505, 522
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 70, 150
CfoI GCGC 1 cut(s) 566
Csp6I GTAC 1 cut(s) 466
CviAII CATG 4 cut(s) 238, 422, 457, 493
CviJI RGCY 3 cut(s) 38, 353, 455
CviKI_1 RGCY 3 cut(s) 38, 353, 455
CviQI GTAC 1 cut(s) 466
DpnI GATC 3 cut(s) 159, 177, 413
DpnII GATC 3 cut(s) 157, 175, 411
DraI TTTAAA 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 193
EcoO65I GGTNACC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 456
EcoT22I ATGCAT 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 4 cut(s) 241, 425, 460, 496
FalI AAGNNNNNCTT 4 cut(s) 255, 287, 339, 371
FaqI GGGAC 2 cut(s) 81, 532
FatI CATG 4 cut(s) 237, 421, 456, 492
FauNDI CATATG 1 cut(s) 406
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 351
FokI GGATG 3 cut(s) 293, 512, 529
Fsp4HI GCNGC 1 cut(s) 351
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 565
GluI GCNGC 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 567
HapII CCGG 1 cut(s) 497
HhaI GCGC 1 cut(s) 566
Hin1II CATG 4 cut(s) 241, 425, 460, 496
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HinfI GANTC 2 cut(s) 418, 489
HpaII CCGG 1 cut(s) 497
HphI GGTGA 2 cut(s) 35, 187
Hpy166II GTNNAC 3 cut(s) 43, 77, 376
Hpy188I TCNGA 3 cut(s) 210, 232, 276
Hpy188III TCNNGA 2 cut(s) 179, 415
Hpy8I GTNNAC 3 cut(s) 43, 77, 376
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 3 cut(s) 74, 218, 470
HpyCH4V TGCA 5 cut(s) 113, 143, 327, 359, 432
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
Hsp92II CATG 4 cut(s) 241, 425, 460, 496
HspAI GCGC 1 cut(s) 564
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 3 cut(s) 157, 175, 411
LpnPI CCDG 8 cut(s) 50, 152, 192, 364, 371, 405, 428, 510
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 5 cut(s) 100, 130, 258, 315, 490
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 3 cut(s) 193, 299, 425
MalI GATC 3 cut(s) 159, 177, 413
MboI GATC 3 cut(s) 157, 175, 411
MboII GAAGA 4 cut(s) 275, 355, 453, 527
MflI RGATCY 2 cut(s) 175, 411
MluCI AATT 2 cut(s) 15, 540
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 2 cut(s) 320, 326
Mph1103I ATGCAT 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 2 cut(s) 225, 479
MspI CCGG 1 cut(s) 497
Mva1269I GAATGC 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 356
NcoI CCATGG 1 cut(s) 456
NdeI CATATG 1 cut(s) 406
NdeII GATC 3 cut(s) 157, 175, 411
NlaIII CATG 4 cut(s) 241, 425, 460, 496
NlaIV GGNNCC 3 cut(s) 177, 390, 413
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 115
PctI GAATGC 1 cut(s) 113
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 2 cut(s) 418, 489
PkrI GCNGC 1 cut(s) 352
PspEI GGTNACC 1 cut(s) 193
PspN4I GGNNCC 3 cut(s) 177, 390, 413
PsuI RGATCY 2 cut(s) 175, 411
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
SaqAI TTAA 2 cut(s) 225, 479
SatI GCNGC 1 cut(s) 351
Sau3AI GATC 3 cut(s) 157, 175, 411
SetI ASST 6 cut(s) 40, 48, 130, 187, 200, 438
SfaNI GCATC 5 cut(s) 100, 130, 258, 315, 490
SfcI CTRYAG 1 cut(s) 199
Sse9I AATT 2 cut(s) 15, 540
SspMI CTAG 1 cut(s) 20
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 3 cut(s) 74, 218, 470
TasI AATT 2 cut(s) 15, 540
TfiI GAWTC 2 cut(s) 418, 489
Tru1I TTAA 2 cut(s) 225, 479
Tru9I TTAA 2 cut(s) 225, 479
TscAI CASTG 2 cut(s) 77, 150
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 5 cut(s) 48, 276, 353, 356, 410
TspRI CASTG 2 cut(s) 77, 150
XapI RAATTY 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 1 cut(s) 20
Zsp2I ATGCAT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.