Rh7AG392300

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0022815
Physical Location & Seq
Forward (+)
51421842 .. 51422525
684 bp
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UTR
Exon/CDS
Intron
Rh7AG392300.1

Sequence Viewer

Length: 684 bp
ATGCTTTTCAAAGAAGGTTATTTAGAAGCTGCACAGACACTCTTTGAACAACTTACTGAAAGAGGGCTACGACCTGATATGGTTACTTACAATACAATGGTTTGTGGTTATTGTTCTCTGAGAAGGTTAGATGAAGCTGTTCACCTTTTTCAACAAATGACACAGGGACAGTGTAAACCCAATGCCATCACTTGTAGTATATTGATCGATGCATTCTGTAAAGAAGGTAAAATGGACGATGCAGTGTTATTGTTTGATCAAATGCTGGAAAAGGATCCTGAACCTAATGTGGTCACCTATAGTTGTCTGATTGATGGTTATTTTAAATCTGAAAACATGGAAAGTGCCTTTGAACTATATGAAGAGATGCTTCAGAGTATCTCTCCAAATATAGTTAGTTACAGCATCCTCATTGATGGTCTTTGCAAAAGAGGACTGATGAAGAAAGCAGCCCTTGCATTTCATTCTGCTATAAATAGGGGTCTGGTGCCGGATGTAGTAGCATATGGGATCCTGATTCATGGTTACTGCAAGGTTGGAAGAACGGCAGAAGCCATGGCATTGTACGGTCGTATGTTAATAAGTGGAATCATGCCGGATGCTATCATACAAAGGATGATTGCGAAGCACCTTCTTGAAATTCGGTCCCACCAAAATATGAGCGCCCAACCAGATGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

227

Amino Acids

25.52

Weight (kDa)

5.4

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 2 - 38 1.3e-08 PPR repeat family
TPR_24 PF23276 7 - 123 3.4e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 14 - 74 1.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 21 - 54 1.7e-12 PPR repeat
PPR_2 PF13041 25 - 74 5.1e-18 PPR repeat family
PPR PF01535 28 - 55 7.4e-08 PPR repeat
PPR_1 PF12854 58 - 88 1.3e-11 PPR repeat
PPR PF01535 63 - 91 7.4e-08 PPR repeat
PPR_2 PF13041 66 - 109 3.1e-14 PPR repeat family
PPR_3 PF13812 84 - 139 9.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 94 - 123 2e-10 PPR repeat
PPR_2 PF13041 95 - 143 2.2e-18 PPR repeat family
PPR PF01535 98 - 125 1.3e-07 PPR repeat
PPR_1 PF12854 127 - 150 1.6e-08 PPR repeat
PPR_2 PF13041 129 - 178 3.6e-13 PPR repeat family
PPR_1 PF12854 160 - 192 5.1e-11 PPR repeat
PPR_2 PF13041 164 - 201 2e-09 PPR repeat family
PPR PF01535 167 - 197 1.2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022815)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0227251 RchiOBHm_Chr7g0227341
rosa_samantha Rh7AG392300 Rh7CG411400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 487
AclWI GGATC 4 cut(s) 269, 282, 505, 518
AcsI RAATTY 1 cut(s) 639
AcuI CTGAAG 1 cut(s) 356
AfaI GTAC 1 cut(s) 566
AgsI TTSAA 5 cut(s) 10, 47, 152, 353, 638
AjuI GAANNNNNNNTTGG 1 cut(s) 660
AluBI AGCT 2 cut(s) 29, 137
AluI AGCT 2 cut(s) 29, 137
AlwI GGATC 4 cut(s) 269, 282, 505, 518
ApeKI GCWGC 2 cut(s) 29, 449
ApoI RAATTY 1 cut(s) 639
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 665
AspS9I GGNCC 1 cut(s) 645
AsuHPI GGTGA 2 cut(s) 134, 286
AvaII GGWCC 1 cut(s) 645
BamHI GGATCC 2 cut(s) 274, 510
BanI GGYRCC 1 cut(s) 487
BbvI GCAGC 2 cut(s) 16, 461
BccI CCATC 3 cut(s) 194, 308, 410
BceAI ACGGC 1 cut(s) 561
BclI TGATCA 1 cut(s) 256
BfmI CTRYAG 1 cut(s) 298
BfoI RGCGCY 1 cut(s) 666
BisI GCNGC 2 cut(s) 30, 450
BlsI GCNGC 2 cut(s) 31, 451
Bme18I GGWCC 1 cut(s) 645
BmgT120I GGNCC 1 cut(s) 645
BmiI GGNNCC 4 cut(s) 276, 489, 512, 647
BmsI GCATC 5 cut(s) 199, 229, 357, 414, 589
BplI GAGNNNNNCTC 2 cut(s) 367, 399
Bsa29I ATCGAT 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 555
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 555
BseGI GGATG 4 cut(s) 405, 499, 604, 621
BseMII CTCAG 1 cut(s) 110
BseXI GCAGC 2 cut(s) 16, 461
BsgI GTGCAG 1 cut(s) 15
Bsh1285I CGRYCG 1 cut(s) 571
BshNI GGYRCC 1 cut(s) 487
BshVI ATCGAT 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 571
BsiSI CCGG 2 cut(s) 491, 596
BslFI GGGAC 2 cut(s) 180, 631
BsmFI GGGAC 2 cut(s) 180, 631
BsmI GAATGC 1 cut(s) 212
Bsp143I GATC 4 cut(s) 204, 256, 274, 510
Bsp19I CCATGG 1 cut(s) 555
BspCNI CTCAG 1 cut(s) 111
BspDI ATCGAT 1 cut(s) 207
BspLI GGNNCC 4 cut(s) 276, 489, 512, 647
BspPI GGATC 4 cut(s) 269, 282, 505, 518
BspT107I GGYRCC 1 cut(s) 487
BssECI CCNNGG 1 cut(s) 555
BssMI GATC 4 cut(s) 204, 256, 274, 510
BssT1I CCWWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 171, 569
Bst6I CTCTTC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 455
BstDEI CTNAG 1 cut(s) 119
BstDSI CCRYGG 1 cut(s) 555
BstEII GGTNACC 1 cut(s) 292
BstF5I GGATG 4 cut(s) 405, 499, 604, 621
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 1 cut(s) 665
BstKTI GATC 4 cut(s) 207, 259, 277, 513
BstMBI GATC 4 cut(s) 204, 256, 274, 510
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstPI GGTNACC 1 cut(s) 292
BstSFI CTRYAG 1 cut(s) 298
BstV1I GCAGC 2 cut(s) 16, 461
BstX2I RGATCY 2 cut(s) 274, 510
BstYI RGATCY 2 cut(s) 274, 510
Bsu15I ATCGAT 1 cut(s) 207
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 4 cut(s) 405, 499, 604, 621
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 176, 249
CfoI GCGC 1 cut(s) 665
Cfr13I GGNCC 1 cut(s) 645
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 1 cut(s) 565
CviAII CATG 4 cut(s) 337, 521, 556, 592
CviJI RGCY 5 cut(s) 29, 67, 137, 452, 554
CviKI_1 RGCY 5 cut(s) 29, 67, 137, 452, 554
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 1 cut(s) 119
DpnI GATC 4 cut(s) 206, 258, 276, 512
DpnII GATC 4 cut(s) 204, 256, 274, 510
DraI TTTAAA 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 356
Eco91I GGTNACC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 555
EcoT22I ATGCAT 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 4 cut(s) 340, 524, 559, 595
FalI AAGNNNNNCTT 4 cut(s) 354, 386, 438, 470
FaqI GGGAC 2 cut(s) 180, 631
FatI CATG 4 cut(s) 336, 520, 555, 591
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 2 cut(s) 30, 450
FokI GGATG 4 cut(s) 392, 506, 611, 628
Fsp4HI GCNGC 2 cut(s) 30, 450
GlaI GCGC 1 cut(s) 664
GluI GCNGC 2 cut(s) 30, 450
HaeII RGCGCY 1 cut(s) 666
HapII CCGG 2 cut(s) 491, 596
HhaI GCGC 1 cut(s) 665
Hin1II CATG 4 cut(s) 340, 524, 559, 595
Hin6I GCGC 1 cut(s) 663
HinP1I GCGC 1 cut(s) 663
HinfI GANTC 2 cut(s) 517, 588
HpaII CCGG 2 cut(s) 491, 596
HphI GGTGA 2 cut(s) 134, 286
Hpy166II GTNNAC 2 cut(s) 142, 176
Hpy188I TCNGA 4 cut(s) 120, 309, 331, 375
Hpy188III TCNNGA 3 cut(s) 278, 514, 635
Hpy8I GTNNAC 2 cut(s) 142, 176
HpyAV CCTTC 4 cut(s) 8, 117, 218, 641
HpyCH4III ACNGT 2 cut(s) 171, 569
HpyCH4V TGCA 6 cut(s) 32, 212, 242, 426, 458, 531
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpyF3I CTNAG 1 cut(s) 119
Hsp92II CATG 4 cut(s) 340, 524, 559, 595
HspAI GCGC 1 cut(s) 663
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 4 cut(s) 204, 256, 274, 510
LpnPI CCDG 8 cut(s) 87, 149, 251, 291, 470, 504, 527, 609
Lsp1109I GCAGC 2 cut(s) 16, 461
LweI GCATC 5 cut(s) 199, 229, 357, 414, 589
MaeIII GTNAC 4 cut(s) 82, 292, 398, 524
MalI GATC 4 cut(s) 206, 258, 276, 512
MboI GATC 4 cut(s) 204, 256, 274, 510
MboII GAAGA 3 cut(s) 374, 454, 552
MflI RGATCY 2 cut(s) 274, 510
MluCI AATT 1 cut(s) 639
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 3 cut(s) 56, 419, 425
Mph1103I ATGCAT 1 cut(s) 214
MroXI GAANNNNTTC 1 cut(s) 138
MseI TTAA 2 cut(s) 324, 578
MspI CCGG 2 cut(s) 491, 596
Mva1269I GAATGC 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 455
NcoI CCATGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 505
NdeII GATC 4 cut(s) 204, 256, 274, 510
NlaIII CATG 4 cut(s) 340, 524, 559, 595
NlaIV GGNNCC 4 cut(s) 276, 489, 512, 647
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 214
PctI GAATGC 1 cut(s) 212
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 517, 588
PkrI GCNGC 2 cut(s) 31, 451
PspEI GGTNACC 1 cut(s) 292
PspN4I GGNNCC 4 cut(s) 276, 489, 512, 647
PspPI GGNCC 1 cut(s) 645
PsuI RGATCY 2 cut(s) 274, 510
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
SaqAI TTAA 2 cut(s) 324, 578
SatI GCNGC 2 cut(s) 30, 450
Sau3AI GATC 4 cut(s) 204, 256, 274, 510
Sau96I GGNCC 1 cut(s) 645
SfaNI GCATC 5 cut(s) 199, 229, 357, 414, 589
SfcI CTRYAG 1 cut(s) 298
SinI GGWCC 1 cut(s) 645
Sse9I AATT 1 cut(s) 639
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 2 cut(s) 171, 569
TaqI TCGA 1 cut(s) 207
TaqII GACCGA 1 cut(s) 633
TasI AATT 1 cut(s) 639
TfiI GAWTC 2 cut(s) 517, 588
Tru1I TTAA 2 cut(s) 324, 578
Tru9I TTAA 2 cut(s) 324, 578
TscAI CASTG 2 cut(s) 176, 249
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 2 cut(s) 29, 449
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 5 cut(s) 147, 375, 452, 455, 509
TspRI CASTG 2 cut(s) 176, 249
VpaK11BI GGWCC 1 cut(s) 645
XapI RAATTY 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 138
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.