Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0025124

Physical Location & Seq

Genomic Coordinates Forward (+)

63370706 .. 63371617

Gene Structure 912 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ21333

Sequence Viewer

CDS Protein Promoter (2k)

Length: 912 bp

ATGACATGTCGTACCAAGTGCGGATCACTCCCTCCGGCGACCTTGAAACAGTGGGGCCGCTACGACTTCGAAACACAACTCGGGTCCATAATGATCGCCCACCCGAAAGTCGACCAGGTGTCCGGCCACCTCCACACGCTCAGCTACGACTTCGTGAAGAGGCCTTACCTGAAATACTTCCAATTCTCCGCCGACGGGGAGAAGTCTCCAAACGTGGAAATCCCTCTGGCGGCGCCCACAATGATGCACGACTTCACCATCATCGAAAACTTCGTCGTGATTCCCGACCAGCAGGTGGTGTTCAAGCTGCAGGAGATGATCACCGACGACCCGCCGGTGATCTACAACAAGAACAAGAAATCCAGGTTAGGAATTCTGGAGAAAAATGCCACAAACGCTGATGATATTATTTGGGTTGAATGCCCGGACACTTTCTGCTTCCACTTGTGGAATGCGTGGGAGGAGCTTCAGTCTGATGAGGTGGTTGTGATCAGGTCATGCATGACTCCACCAGACTCGATATTTAATGAGAATGACGAGTGCTTGAAGAGTGTGTTGTCCGAAATCCGGCTCAATATGAAAACCGGCGAGTCGACTCGGAGGCCGATTATTTCGGAGGAGTTGGAGCATGTGAACTTAGAGGCCGGAATGGTGAATCGGAACCGGCTCGGGAGAAAAACTCGGTATGCTTATCTCGCCATTGCTGAACCGTGGCCGAAAGTTTCTAGCTTCGCCAAGGTGGATCTTTTTACTGGCCAAGTAAAAAAGCATATTTACGGGGATAGGAAGTTTGGTGGTGAGCCCTTCTTTCTTCCTTCGGGTTCATGTGGGGAAGAAGATGGTGGGTATATCCTGAGCTTTGTTCATGACGAGAAGACGTGGAAGTCGGAGCTTCAGATTGTGAATGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009687 GO:0009688 GO:0009791 GO:0009987 GO:0010035 GO:0010154 GO:0010162 GO:0010431 GO:0010436 GO:0016020 GO:0016053 GO:0016106 GO:0016114 GO:0016491 GO:0016701 GO:0016702 GO:0019752 GO:0021700 GO:0022414 GO:0022611 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034357 GO:0042221 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045549 GO:0046165 GO:0046394 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051213 GO:0055035 GO:0055114 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

RPE65

Protein Analysis

303

Amino Acids

34.62

Weight (kDa)

5.56

Isoelectric Point (pI)

46.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPE65	PF03055	10 - 303	7e-69	Retinal pigment epithelial membrane protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0025124)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr7g0238031
rosa_multiflora	Rmu_sc0007698.1_g000020 Rmu_sc0030207.1_g000002

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	283
AasI	GACNNNNNNGTC	1 cut(s)	883
Acc36I	ACCTGC	1 cut(s)	283
AccB1I	GGYRCC	1 cut(s)	232
AccB7I	CCANNNNNTGG	1 cut(s)	295
AccI	GTMKAC	2 cut(s)	111, 593
AciI	CCGC	5 cut(s)	21, 58, 189, 230, 332
AclWI	GGATC	2 cut(s)	31, 750
AcoI	YGGCCR	3 cut(s)	124, 713, 754
AcsI	RAATTY	1 cut(s)	372
AcuI	CTGAAG	2 cut(s)	452, 878
AcyI	GRCGYC	1 cut(s)	233
AfaI	GTAC	1 cut(s)	13
AfiI	CCNNNNNNNGG	4 cut(s)	195, 229, 295, 567
AflIII	ACRYGT	1 cut(s)	5
AgsI	TTSAA	4 cut(s)	46, 304, 419, 547
AhdI	GACNNNNNGTC	1 cut(s)	118
AjiI	CACGTC	1 cut(s)	879
AjnI	CCWGG	2 cut(s)	114, 362
AloI	GAACNNNNNNTCC	2 cut(s)	344, 376
AluBI	AGCT	6 cut(s)	144, 307, 466, 729, 858, 892
AluI	AGCT	6 cut(s)	144, 307, 466, 729, 858, 892
Alw26I	GTCTC	1 cut(s)	210
AlwI	GGATC	2 cut(s)	31, 750
Ama87I	CYCGRG	2 cut(s)	80, 668
AoxI	GGCC	7 cut(s)	55, 124, 161, 602, 642, 713, 754
ApeKI	GCWGC	1 cut(s)	307
ApoI	RAATTY	1 cut(s)	372
ArsI	GACNNNNNNTTYG	2 cut(s)	258, 290
Asp700I	GAANNNNTTC	1 cut(s)	176
AspLEI	GCGC	1 cut(s)	235
AspS9I	GGNCC	2 cut(s)	55, 84
AsuC2I	CCSGG	1 cut(s)	425
AsuHPI	GGTGA	5 cut(s)	247, 313, 349, 664, 809
AsuII	TTCGAA	1 cut(s)	69
AvaI	CYCGRG	2 cut(s)	80, 668
AvaII	GGWCC	1 cut(s)	84
BalI	TGGCCA	1 cut(s)	756
BanI	GGYRCC	1 cut(s)	232
BanII	GRGCYC	1 cut(s)	804
BarI	GAAGNNNNNNTAC	2 cut(s)	149, 181
BbsI	GAAGAC	1 cut(s)	881
BbvI	GCAGC	1 cut(s)	294
BccI	CCATC	2 cut(s)	266, 833
BciT130I	CCWGG	2 cut(s)	116, 364
BclI	TGATCA	2 cut(s)	318, 489
BcnI	CCSGG	1 cut(s)	425
BcoDI	GTCTC	1 cut(s)	210
BfaI	CTAG	1 cut(s)	726
BfmI	CTRYAG	1 cut(s)	308
BfoI	RGCGCY	1 cut(s)	236
BfuAI	ACCTGC	1 cut(s)	283
BisI	GCNGC	3 cut(s)	58, 231, 308
BlpI	GCTNAGC	1 cut(s)	140
BlsI	GCNGC	3 cut(s)	59, 232, 309
Bme1390I	CCNGG	3 cut(s)	116, 364, 425
Bme18I	GGWCC	1 cut(s)	84
BmeRI	GACNNNNNGTC	1 cut(s)	118
BmeT110I	CYCGRG	2 cut(s)	80, 668
BmgBI	CACGTC	1 cut(s)	879
BmgT120I	GGNCC	2 cut(s)	55, 84
BmiI	GGNNCC	4 cut(s)	56, 85, 234, 662
BmrFI	CCNGG	3 cut(s)	116, 364, 425
BmsI	GCATC	1 cut(s)	234
BpiI	GAAGAC	1 cut(s)	881
BplI	GAGNNNNNCTC	2 cut(s)	664, 696
BpmI	CTGGAG	1 cut(s)	398
Bpu10I	CCTNAGC	1 cut(s)	854
Bpu1102I	GCTNAGC	1 cut(s)	140
Bpu14I	TTCGAA	1 cut(s)	69
BpuMI	CCSGG	1 cut(s)	425
BsaHI	GRCGYC	1 cut(s)	233
BsaJI	CCNNGG	2 cut(s)	710, 735
BsaXI	ACNNNNNCTCC	2 cut(s)	455, 485
Bsc4I	CCNNNNNNNGG	4 cut(s)	195, 229, 295, 567
Bse118I	RCCGGY	3 cut(s)	334, 584, 663
Bse1I	ACTGG	1 cut(s)	757
Bse3DI	GCAATG	1 cut(s)	699
BseBI	CCWGG	2 cut(s)	116, 364
BseDI	CCNNGG	2 cut(s)	710, 735
BseLI	CCNNNNNNNGG	4 cut(s)	195, 229, 295, 567
BseMI	GCAATG	1 cut(s)	699
BseMII	CTCAG	2 cut(s)	154, 845
BseNI	ACTGG	1 cut(s)	757
BseRI	GAGGAG	2 cut(s)	476, 632
BseXI	GCAGC	1 cut(s)	294
BshFI	GGCC	7 cut(s)	57, 126, 163, 604, 644, 715, 756
BshNI	GGYRCC	1 cut(s)	232
BsiHKCI	CYCGRG	2 cut(s)	80, 668
BsiSI	CCGG	8 cut(s)	35, 123, 335, 425, 568, 585, 645, 664
BslI	CCNNNNNNNGG	4 cut(s)	195, 229, 295, 567
BsmAI	GTCTC	1 cut(s)	210
BsmI	GAATGC	3 cut(s)	425, 457, 910
BsnI	GGCC	7 cut(s)	57, 126, 163, 604, 644, 715, 756
BsoBI	CYCGRG	2 cut(s)	80, 668
Bsp119I	TTCGAA	1 cut(s)	69
Bsp1286I	GDGCHC	1 cut(s)	804
Bsp143I	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
Bsp1720I	GCTNAGC	1 cut(s)	140
BspACI	CCGC	5 cut(s)	21, 58, 189, 230, 332
BspANI	GGCC	7 cut(s)	57, 126, 163, 604, 644, 715, 756
BspCNI	CTCAG	2 cut(s)	153, 846
BspHI	TCATGA	1 cut(s)	865
BspLI	GGNNCC	4 cut(s)	56, 85, 234, 662
BspMAI	CTGCAG	1 cut(s)	312
BspMI	ACCTGC	1 cut(s)	283
BspPI	GGATC	2 cut(s)	31, 750
BspT104I	TTCGAA	1 cut(s)	69
BspT107I	GGYRCC	1 cut(s)	232
BsrDI	GCAATG	1 cut(s)	699
BsrFI	RCCGGY	3 cut(s)	334, 584, 663
BsrI	ACTGG	1 cut(s)	757
BssAI	RCCGGY	3 cut(s)	334, 584, 663
BssECI	CCNNGG	2 cut(s)	710, 735
BssMI	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
BssNI	GRCGYC	1 cut(s)	233
BssT1I	CCWWGG	1 cut(s)	735
Bst2UI	CCWGG	2 cut(s)	116, 364
Bst4CI	ACNGT	2 cut(s)	51, 711
Bst6I	CTCTTC	2 cut(s)	152, 542
BstACI	GRCGYC	1 cut(s)	233
BstBI	TTCGAA	1 cut(s)	69
BstDEI	CTNAG	3 cut(s)	140, 637, 854
BstDSI	CCRYGG	1 cut(s)	710
BstH2I	RGCGCY	1 cut(s)	236
BstHHI	GCGC	1 cut(s)	235
BstKTI	GATC	6 cut(s)	26, 96, 321, 342, 492, 745
BstMAI	GTCTC	1 cut(s)	210
BstMBI	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
BstMWI	GCNNNNNNNGC	2 cut(s)	395, 695
BstNI	CCWGG	2 cut(s)	116, 364
BstNSI	RCATGY	2 cut(s)	9, 632
BstSCI	CCNGG	3 cut(s)	114, 362, 423
BstSFI	CTRYAG	1 cut(s)	308
BstV1I	GCAGC	1 cut(s)	294
BstV2I	GAAGAC	1 cut(s)	881
BstX2I	RGATCY	1 cut(s)	742
BstYI	RGATCY	1 cut(s)	742
BsuRI	GGCC	7 cut(s)	57, 126, 163, 604, 644, 715, 756
BtgI	CCRYGG	1 cut(s)	710
BtrI	CACGTC	1 cut(s)	879
BtsIMutI	CAGTG	1 cut(s)	56
BveI	ACCTGC	1 cut(s)	283
CciI	TCATGA	1 cut(s)	865
CfoI	GCGC	1 cut(s)	235
Cfr10I	RCCGGY	3 cut(s)	334, 584, 663
Cfr13I	GGNCC	2 cut(s)	55, 84
CsiI	ACCWGGT	1 cut(s)	114
Csp6I	GTAC	1 cut(s)	12
CviAII	CATG	6 cut(s)	6, 498, 502, 629, 825, 866
CviQI	GTAC	1 cut(s)	12
DdeI	CTNAG	3 cut(s)	140, 637, 854
DinI	GGCGCC	1 cut(s)	234
DpnI	GATC	6 cut(s)	25, 95, 320, 341, 491, 744
DpnII	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
DrdI	GACNNNNNNGTC	1 cut(s)	883
DriI	GACNNNNNGTC	1 cut(s)	118
DseDI	GACNNNNNNGTC	1 cut(s)	883
EaeI	YGGCCR	3 cut(s)	124, 713, 754
Eam1104I	CTCTTC	2 cut(s)	152, 542
Eam1105I	GACNNNNNGTC	1 cut(s)	118
EarI	CTCTTC	2 cut(s)	152, 542
EciI	GGCGGA	1 cut(s)	178
Eco130I	CCWWGG	1 cut(s)	735
Eco147I	AGGCCT	1 cut(s)	163
Eco24I	GRGCYC	1 cut(s)	804
Eco47I	GGWCC	1 cut(s)	84
Eco57I	CTGAAG	2 cut(s)	452, 878
Eco88I	CYCGRG	2 cut(s)	80, 668
EcoRI	GAATTC	1 cut(s)	372
EcoRII	CCWGG	2 cut(s)	114, 362
EcoT14I	CCWWGG	1 cut(s)	735
EcoT22I	ATGCAT	1 cut(s)	503
EcoT38I	GRGCYC	1 cut(s)	804
EgeI	GGCGCC	1 cut(s)	234
EheI	GGCGCC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	735
FaeI	CATG	6 cut(s)	9, 501, 505, 632, 828, 869
FatI	CATG	6 cut(s)	5, 497, 501, 628, 824, 865
FauI	CCCGC	1 cut(s)	339
FbaI	TGATCA	2 cut(s)	318, 489
FblI	GTMKAC	2 cut(s)	111, 593
Fnu4HI	GCNGC	3 cut(s)	58, 231, 308
FriOI	GRGCYC	1 cut(s)	804
Fsp4HI	GCNGC	3 cut(s)	58, 231, 308
FspBI	CTAG	1 cut(s)	726
GlaI	GCGC	1 cut(s)	234
GluI	GCNGC	3 cut(s)	58, 231, 308
GsuI	CTGGAG	1 cut(s)	398
HaeII	RGCGCY	1 cut(s)	236
HaeIII	GGCC	7 cut(s)	57, 126, 163, 604, 644, 715, 756
HapII	CCGG	8 cut(s)	35, 123, 335, 425, 568, 585, 645, 664
HhaI	GCGC	1 cut(s)	235
Hin1I	GRCGYC	1 cut(s)	233
Hin1II	CATG	6 cut(s)	9, 501, 505, 632, 828, 869
Hin6I	GCGC	1 cut(s)	233
HinP1I	GCGC	1 cut(s)	233
HincII	GTYRAC	2 cut(s)	112, 594
HindII	GTYRAC	2 cut(s)	112, 594
HinfI	GANTC	6 cut(s)	280, 505, 515, 590, 595, 655
HpaII	CCGG	8 cut(s)	35, 123, 335, 425, 568, 585, 645, 664
HphI	GGTGA	5 cut(s)	247, 313, 349, 664, 809
Hpy166II	GTNNAC	3 cut(s)	112, 594, 634
Hpy188I	TCNGA	7 cut(s)	475, 562, 600, 616, 660, 889, 897
Hpy188III	TCNNGA	7 cut(s)	154, 277, 284, 377, 670, 853, 866
Hpy8I	GTNNAC	3 cut(s)	112, 594, 634
Hpy99I	CGWCG	3 cut(s)	197, 278, 329
HpyAV	CCTTC	2 cut(s)	814, 825
HpyCH4III	ACNGT	2 cut(s)	51, 711
HpyCH4IV	ACGT	2 cut(s)	213, 878
HpyCH4V	TGCA	3 cut(s)	247, 310, 501
HpyF10VI	GCNNNNNNNGC	2 cut(s)	395, 695
HpyF3I	CTNAG	3 cut(s)	140, 637, 854
HpySE526I	ACGT	2 cut(s)	213, 878
Hsp92I	GRCGYC	1 cut(s)	233
Hsp92II	CATG	6 cut(s)	9, 501, 505, 632, 828, 869
HspAI	GCGC	1 cut(s)	233
KasI	GGCGCC	1 cut(s)	232
Ksp22I	TGATCA	2 cut(s)	318, 489
Kzo9I	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
LmnI	GCTCC	3 cut(s)	463, 625, 889
Lsp1109I	GCAGC	1 cut(s)	294
LweI	GCATC	1 cut(s)	234
MabI	ACCWGGT	1 cut(s)	114
MaeI	CTAG	1 cut(s)	726
MaeII	ACGT	2 cut(s)	213, 878
MalI	GATC	6 cut(s)	25, 95, 320, 341, 491, 744
MboI	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
MboII	GAAGA	6 cut(s)	169, 559, 803, 845, 848, 886
MflI	RGATCY	1 cut(s)	742
MhlI	GDGCHC	1 cut(s)	804
MlsI	TGGCCA	1 cut(s)	756
MluCI	AATT	2 cut(s)	182, 372
MluNI	TGGCCA	1 cut(s)	756
Mly113I	GGCGCC	1 cut(s)	233
MlyI	GAGTC	4 cut(s)	499, 509, 589, 599
MmeI	TCCRAC	2 cut(s)	603, 867
MnlI	CCTC	9 cut(s)	42, 140, 153, 234, 454, 472, 594, 610, 634
Mox20I	TGGCCA	1 cut(s)	756
Mph1103I	ATGCAT	1 cut(s)	503
MroXI	GAANNNNTTC	1 cut(s)	176
MscI	TGGCCA	1 cut(s)	756
MseI	TTAA	1 cut(s)	525
MslI	CAYNNNNRTG	1 cut(s)	242
Msp20I	TGGCCA	1 cut(s)	756
MspI	CCGG	8 cut(s)	35, 123, 335, 425, 568, 585, 645, 664
MspR9I	CCNGG	3 cut(s)	116, 364, 425
Mva1269I	GAATGC	3 cut(s)	425, 457, 910
MvaI	CCWGG	2 cut(s)	116, 364
MwoI	GCNNNNNNNGC	2 cut(s)	395, 695
NarI	GGCGCC	1 cut(s)	233
NciI	CCSGG	1 cut(s)	425
NdeII	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
NlaIII	CATG	6 cut(s)	9, 501, 505, 632, 828, 869
NlaIV	GGNNCC	4 cut(s)	56, 85, 234, 662
NsiI	ATGCAT	1 cut(s)	503
NspI	RCATGY	2 cut(s)	9, 632
NspV	TTCGAA	1 cut(s)	69
PagI	TCATGA	1 cut(s)	865
PaqCI	CACCTGC	1 cut(s)	283
PceI	AGGCCT	1 cut(s)	163
PciI	ACATGT	1 cut(s)	5
PcsI	WCGNNNNNNNCGW	2 cut(s)	270, 282
PctI	GAATGC	3 cut(s)	425, 457, 910
PdmI	GAANNNNTTC	1 cut(s)	176
PfeI	GAWTC	2 cut(s)	280, 655
PflMI	CCANNNNNTGG	1 cut(s)	295
PkrI	GCNGC	3 cut(s)	59, 232, 309
PleI	GAGTC	4 cut(s)	499, 509, 589, 598
PluTI	GGCGCC	1 cut(s)	236
PpsI	GAGTC	4 cut(s)	499, 509, 589, 598
PscI	ACATGT	1 cut(s)	5
Psp6I	CCWGG	2 cut(s)	114, 362
PspGI	CCWGG	2 cut(s)	114, 362
PspN4I	GGNNCC	4 cut(s)	56, 85, 234, 662
PspPI	GGNCC	2 cut(s)	55, 84
PstI	CTGCAG	1 cut(s)	312
PsuI	RGATCY	1 cut(s)	742
RsaI	GTAC	1 cut(s)	13
RsaNI	GTAC	1 cut(s)	12
RseI	CAYNNNNRTG	1 cut(s)	242
SalI	GTCGAC	2 cut(s)	110, 592
SaqAI	TTAA	1 cut(s)	525
SatI	GCNGC	3 cut(s)	58, 231, 308
Sau3AI	GATC	6 cut(s)	23, 93, 318, 339, 489, 742
Sau96I	GGNCC	2 cut(s)	55, 84
SchI	GAGTC	4 cut(s)	499, 509, 589, 599
ScrFI	CCNGG	3 cut(s)	116, 364, 425
SduI	GDGCHC	1 cut(s)	804
SexAI	ACCWGGT	1 cut(s)	114
SfaNI	GCATC	1 cut(s)	234
SfcI	CTRYAG	1 cut(s)	308
SfoI	GGCGCC	1 cut(s)	234
SfuI	TTCGAA	1 cut(s)	69
SgrAI	CRCCGGYG	1 cut(s)	334
SinI	GGWCC	1 cut(s)	84
SmiMI	CAYNNNNRTG	1 cut(s)	242
Sse9I	AATT	2 cut(s)	182, 372
SseBI	AGGCCT	1 cut(s)	163
SsiI	CCGC	5 cut(s)	21, 58, 189, 230, 332
SspDI	GGCGCC	1 cut(s)	232
SspMI	CTAG	1 cut(s)	726
StuI	AGGCCT	1 cut(s)	163
StyD4I	CCNGG	3 cut(s)	114, 362, 423
StyI	CCWWGG	1 cut(s)	735
TaaI	ACNGT	2 cut(s)	51, 711
TaiI	ACGT	2 cut(s)	216, 881
TaqI	TCGA	5 cut(s)	69, 111, 264, 518, 593
TasI	AATT	2 cut(s)	182, 372
TauI	GCSGC	2 cut(s)	60, 233
TfiI	GAWTC	2 cut(s)	280, 655
Tru1I	TTAA	1 cut(s)	525
Tru9I	TTAA	1 cut(s)	525
TscAI	CASTG	1 cut(s)	56
TseI	GCWGC	1 cut(s)	307
TspDTI	ATGAA	3 cut(s)	593, 813, 854
TspRI	CASTG	1 cut(s)	56
Van91I	CCANNNNNTGG	1 cut(s)	295
VpaK11BI	GGWCC	1 cut(s)	84
XapI	RAATTY	1 cut(s)	372
XceI	RCATGY	2 cut(s)	9, 632
XmiI	GTMKAC	2 cut(s)	111, 593
XmnI	GAANNNNTTC	1 cut(s)	176
XspI	CTAG	1 cut(s)	726
Zsp2I	ATGCAT	1 cut(s)	503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.