RLG00000000432

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0019171
Physical Location & Seq
Reverse (-)
962663 .. 963232
570 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000432

Sequence Viewer

Length: 372 bp
ATGGAAACAGCAACTAACTATGGCTCTTGGCCCAGACAACGTCCTAGGCGTAGGGGAGATCGGAGCAACAGGGAACGCCTAGACGACCTCCAATCTGACCTAGCTGGCGAGCGATCCCAGTTCGCGGCAGAGAACAAGACAGCAAGCGAGCGTACCTTTGTTCGCTTCTTCTCCACCAAGCACGGAAGTTTAAGGATAACTGTAGGTCGGTGGCTACCTAAGGAAACTCCGATTCGATCCGCCCCCCGGCTGGGAGGCATCTACCTCATCCCGATCACAAGGAGAGGGGGCCTCAGGCCCTACTTAGTTTCGCAAGCCTTTGTCATTGTCAGTCAACTAAGTAGGGCGCCTTTTGAATTTAATCTTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

14.16

Weight (kDa)

11.46

Isoelectric Point (pI)

62.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07599 AT2G07599 ATMG01040
rosa_chinensis RchiOBHm_Chr6g0256741 RchiOBHm_MTg0498811
rosa_laevigata RLG00000000148 RLG00000000432 RLG00000000493

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccII CGCG 1 cut(s) 125
AciI CCGC 2 cut(s) 125, 240
AclWI GGATC 2 cut(s) 108, 231
AcsI RAATTY 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 347
AfaI GTAC 1 cut(s) 154
AfiI CCNNNNNNNGG 4 cut(s) 124, 246, 250, 251
AflII CTTAAG 1 cut(s) 365
AgsI TTSAA 1 cut(s) 356
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
AlwI GGATC 2 cut(s) 108, 231
AoxI GGCC 3 cut(s) 29, 289, 296
ApoI RAATTY 1 cut(s) 356
AspA2I CCTAGG 1 cut(s) 44
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 3 cut(s) 30, 289, 297
AsuC2I CCSGG 1 cut(s) 247
AvrII CCTAGG 1 cut(s) 44
AxyI CCTNAGG 2 cut(s) 219, 293
BanI GGYRCC 1 cut(s) 346
BcnI CCSGG 1 cut(s) 247
BfaI CTAG 3 cut(s) 45, 80, 101
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 350
BfrI CTTAAG 1 cut(s) 365
BisI GCNGC 1 cut(s) 126
BlnI CCTAGG 1 cut(s) 44
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 1 cut(s) 247
BmgT120I GGNCC 3 cut(s) 30, 289, 297
BmiI GGNNCC 2 cut(s) 290, 348
BmrFI CCNGG 1 cut(s) 247
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 267
BmuI ACTGGG 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 276, 308
BpuMI CCSGG 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 347
BsaJI CCNNGG 2 cut(s) 44, 245
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 246, 250, 251
Bse1I ACTGG 1 cut(s) 118
Bse21I CCTNAGG 2 cut(s) 219, 293
BseDI CCNNGG 2 cut(s) 44, 245
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 4 cut(s) 124, 246, 250, 251
BseMII CTCAG 1 cut(s) 307
BseNI ACTGG 1 cut(s) 118
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 3 cut(s) 31, 291, 298
BshNI GGYRCC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 247
BslI CCNNNNNNNGG 4 cut(s) 124, 246, 250, 251
BsnI GGCC 3 cut(s) 31, 291, 298
Bsp143I GATC 4 cut(s) 58, 113, 236, 273
BspACI CCGC 2 cut(s) 125, 240
BspANI GGCC 3 cut(s) 31, 291, 298
BspCNI CTCAG 1 cut(s) 306
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 2 cut(s) 290, 348
BspPI GGATC 2 cut(s) 108, 231
BspT107I GGYRCC 1 cut(s) 346
BspTI CTTAAG 1 cut(s) 365
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 44, 245
BssMI GATC 4 cut(s) 58, 113, 236, 273
BssNI GRCGYC 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 202
BstACI GRCGYC 1 cut(s) 347
BstAFI CTTAAG 1 cut(s) 365
BstC8I GCNNGC 5 cut(s) 106, 110, 145, 149, 315
BstDEI CTNAG 4 cut(s) 219, 293, 304, 338
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 4 cut(s) 61, 116, 239, 276
BstMBI GATC 4 cut(s) 58, 113, 236, 273
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 1 cut(s) 125
Bsu36I CCTNAGG 2 cut(s) 219, 293
BsuRI GGCC 3 cut(s) 31, 291, 298
BtsCI GGATG 1 cut(s) 267
Cac8I GCNNGC 5 cut(s) 106, 110, 145, 149, 315
CfoI GCGC 1 cut(s) 349
Cfr13I GGNCC 3 cut(s) 30, 289, 297
Csp6I GTAC 1 cut(s) 153
CviJI RGCY 8 cut(s) 24, 31, 104, 214, 250, 291, 298, 317
CviKI_1 RGCY 8 cut(s) 24, 31, 104, 214, 250, 291, 298, 317
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 4 cut(s) 219, 293, 304, 338
DinI GGCGCC 1 cut(s) 348
DpnI GATC 4 cut(s) 60, 115, 238, 275
DpnII GATC 4 cut(s) 58, 113, 236, 273
EciI GGCGGA 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 44
Eco81I CCTNAGG 2 cut(s) 219, 293
EcoO109I RGGNCCY 2 cut(s) 289, 297
EcoT14I CCWWGG 1 cut(s) 44
EgeI GGCGCC 1 cut(s) 348
EheI GGCGCC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 44
FaiI YATR 1 cut(s) 21
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 3 cut(s) 45, 80, 101
GlaI GCGC 1 cut(s) 348
GluI GCNGC 1 cut(s) 126
GsaI CCCAGC 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 3 cut(s) 31, 291, 298
HapII CCGG 1 cut(s) 247
HhaI GCGC 1 cut(s) 349
Hin1I GRCGYC 1 cut(s) 347
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 335
HindII GTYRAC 1 cut(s) 335
HinfI GANTC 1 cut(s) 232
HpaII CCGG 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 3 cut(s) 63, 97, 231
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 40
HpyF3I CTNAG 4 cut(s) 219, 293, 304, 338
HpySE526I ACGT 1 cut(s) 40
Hsp92I GRCGYC 1 cut(s) 347
HspAI GCGC 1 cut(s) 347
KasI GGCGCC 1 cut(s) 346
Kzo9I GATC 4 cut(s) 58, 113, 236, 273
LmnI GCTCC 1 cut(s) 63
LpnPI CCDG 7 cut(s) 46, 55, 90, 131, 236, 260, 280
LweI GCATC 1 cut(s) 267
MaeI CTAG 3 cut(s) 45, 80, 101
MaeII ACGT 1 cut(s) 40
MalI GATC 4 cut(s) 60, 115, 238, 275
MboI GATC 4 cut(s) 58, 113, 236, 273
MboII GAAGA 1 cut(s) 160
MluCI AATT 1 cut(s) 356
Mly113I GGCGCC 1 cut(s) 347
MnlI CCTC 5 cut(s) 98, 248, 275, 278, 302
MseI TTAA 3 cut(s) 191, 360, 366
MspCI CTTAAG 1 cut(s) 365
MspI CCGG 1 cut(s) 247
MspR9I CCNGG 1 cut(s) 247
MvnI CGCG 1 cut(s) 125
NarI GGCGCC 1 cut(s) 347
NciI CCSGG 1 cut(s) 247
NdeII GATC 4 cut(s) 58, 113, 236, 273
NlaIV GGNNCC 2 cut(s) 290, 348
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PfeI GAWTC 1 cut(s) 232
PflFI GACNNNGTC 1 cut(s) 39
PkrI GCNGC 1 cut(s) 127
PluTI GGCGCC 1 cut(s) 350
PspFI CCCAGC 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 290, 348
PspPI GGNCC 3 cut(s) 30, 289, 297
PsyI GACNNNGTC 1 cut(s) 39
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
SaqAI TTAA 3 cut(s) 191, 360, 366
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 4 cut(s) 58, 113, 236, 273
Sau96I GGNCC 3 cut(s) 30, 289, 297
ScrFI CCNGG 1 cut(s) 247
SetI ASST 8 cut(s) 43, 90, 102, 106, 158, 208, 220, 267
SfaNI GCATC 1 cut(s) 267
SfcI CTRYAG 1 cut(s) 201
SfoI GGCGCC 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 1 cut(s) 356
SsiI CCGC 2 cut(s) 125, 240
SspDI GGCGCC 1 cut(s) 346
SspMI CTAG 3 cut(s) 45, 80, 101
StyD4I CCNGG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 235
TasI AATT 1 cut(s) 356
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 3 cut(s) 191, 360, 366
Tru9I TTAA 3 cut(s) 191, 360, 366
TspGWI ACGGA 1 cut(s) 198
Tth111I GACNNNGTC 1 cut(s) 39
Vha464I CTTAAG 1 cut(s) 365
XapI RAATTY 1 cut(s) 356
XmaJI CCTAGG 1 cut(s) 44
XspI CTAG 3 cut(s) 45, 80, 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.