RLG00000000448

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0021922
Physical Location & Seq
Forward (+)
1010653 .. 1013870
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000448

Sequence Viewer

Length: 1194 bp
ATGTCATATATTTCAGGAGCTAGATCAGTTGCCGATGAACAAGTAAGAATTGCCTCAACAAAAATTGATGGAATTGGACCTAAAAAAGCCATTCAGGCCTCCTTCGCCACTCCACTAGTGGCTCGGCTTGGTCTCGCTTCCTTCGCCCGACTATCGTATCGGCTCGGCTTCGATTCCGAGAACGAGCCGGCCGATCCTAACATCATTTATGAAGAGCCGGACGACGCCTCAGATAAAGATAGAGATGCTTTCTATCAGTCAAAAGCGGATGCCGCCCCCCCCATGCTCCCACCGTCCGCTCCCCGAGGAATAGAAAGGGAGGACCGGGGGCCAGAGCTAGTTGGGTTGGGGTATAGAGCCCGCGTTTGGATCTGCGAAGGCAATCGCTCGGATGTTCCCCTAACCCAACCCGGGAACGGACCGGAGGGAACCGCAGCATGGGGAATGTCCGCGTCTCGTCGCAAGGCTCATTTTGAGTTGTGGTTCATAGGGCGGGCAGCTTTATCTGATCAAGGGCCGGGGCACAAGGGTCCTGGTACTATCCAGGTGACCCCACCCCCCCCGAGAGCAGGATGCCGGCCGAGATGGAGCTGGGAGCCAACCTATACTTTCCTGGGGCTTGCCTTGAAAAAACATGTAAATAAAAGACGGGCGCCGAAAAGAAACCAGCCCAGCCTCTTCAAGAAAGCTTTGCTTGCGCCTCGGAATAGCGAAAGCACGGACGAGCCACATGCAGGGAAACTTGCACGTGTGGTTCCGGCCGGGGACCCCGGTCCCCGTAATTCGAGTGAGATTGTCATGGCGCAAAAGCAGATATGGTCCGGTATTCCCTTGTTCCCTGTATTGGTTATGTTCTTCATTTCTCGTCTAGCAGAAACTAATCGAGCTCCGTTTGATCTCCCAGAAGCGGAAGCTGAATCAGTTGCAGGCTATAATGTAGAATATGCGCGGGATGCGATCCTTAATAGTCCACTGTTGGCGGAAGCCAATGTCCCGGGGTCCCGGGGACTCATTCTGACTGAAACAAGGGGTGGGTCTTTACCAACTTTTAAATATTCTATTTTAGGGAAGCCAAAAAACGTAAGCGCCCCTACGCGAGCCTTATTGAAAAGGCCCCTTACTATAGAACAAAGAAAGGGATTGAGCTGCGCGAAAGCCGTTGCGCTAACGCGCATCCGTTTTCTTGCTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
NADHdh

Protein Analysis

398

Amino Acids

43.22

Weight (kDa)

9.83

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADHdh PF00146 261 - 316 2.1e-15 NADH dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021922)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01120
rosa_chinensis RchiOBHm_MTg0499241
rosa_laevigata RLG00000000448 RLG00000000456

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 652
AccBSI CCGCTC 1 cut(s) 299
AccII CGCG 6 cut(s) 363, 452, 949, 1096, 1151, 1171
AclWI GGATC 3 cut(s) 188, 377, 952
AcoI YGGCCR 3 cut(s) 189, 578, 759
AcvI CACGTG 1 cut(s) 749
AcyI GRCGYC 2 cut(s) 225, 653
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 8 cut(s) 366, 411, 416, 438, 569, 734, 844, 907
AflIII ACRYGT 2 cut(s) 634, 748
AgsI TTSAA 3 cut(s) 628, 682, 1108
AhlI ACTAGT 1 cut(s) 115
AjnI CCWGG 3 cut(s) 532, 543, 612
AluBI AGCT 8 cut(s) 20, 337, 500, 591, 689, 887, 914, 1146
AluI AGCT 8 cut(s) 20, 337, 500, 591, 689, 887, 914, 1146
Alw21I GWGCWC 1 cut(s) 889
Alw26I GTCTC 2 cut(s) 137, 459
AlwI GGATC 3 cut(s) 188, 377, 952
Ama87I CYCGRG 5 cut(s) 303, 410, 562, 994, 1002
AoxI GGCC 7 cut(s) 96, 189, 329, 515, 578, 759, 1112
ApeKI GCWGC 3 cut(s) 434, 497, 1146
AspLEI GCGC 8 cut(s) 655, 700, 805, 949, 1088, 1151, 1165, 1173
AsuHPI GGTGA 1 cut(s) 559
AvaI CYCGRG 5 cut(s) 303, 410, 562, 994, 1002
AvaII GGWCC 8 cut(s) 77, 322, 419, 530, 766, 773, 819, 999
BaeGI GKGCMC 1 cut(s) 525
BaeI ACNNNNGTAYC 2 cut(s) 140, 173
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 2 cut(s) 361, 889
BbrPI CACGTG 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 889
BbvI GCAGC 3 cut(s) 446, 509, 1133
BccI CCATC 2 cut(s) 62, 579
BceAI ACGGC 1 cut(s) 1142
BcgI CGANNNNNNTGC 2 cut(s) 713, 747
BciT130I CCWGG 3 cut(s) 534, 545, 614
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 2 cut(s) 137, 459
BcuI ACTAGT 1 cut(s) 115
BfaI CTAG 4 cut(s) 21, 116, 338, 869
BfmI CTRYAG 1 cut(s) 1122
BfoI RGCGCY 2 cut(s) 656, 1089
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 4 cut(s) 273, 435, 498, 1147
BlsI GCNGC 4 cut(s) 274, 436, 499, 1148
Bme18I GGWCC 8 cut(s) 77, 322, 419, 530, 766, 773, 819, 999
BmeT110I CYCGRG 5 cut(s) 303, 410, 562, 994, 1002
BmsI GCATC 5 cut(s) 235, 259, 563, 943, 1182
BoxI GACNNNNGTC 1 cut(s) 771
BsaAI YACGTR 1 cut(s) 749
BsaHI GRCGYC 2 cut(s) 225, 653
BsaI GGTCTC 1 cut(s) 137
BsaWI WCCGGW 2 cut(s) 421, 821
Bsc4I CCNNNNNNNGG 8 cut(s) 366, 411, 416, 438, 569, 734, 844, 907
Bse118I RCCGGY 2 cut(s) 187, 576
BseBI CCWGG 3 cut(s) 534, 545, 614
BseGI GGATG 5 cut(s) 274, 397, 578, 958, 1173
BseLI CCNNNNNNNGG 8 cut(s) 366, 411, 416, 438, 569, 734, 844, 907
BseMII CTCAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 525
BseX3I CGGCCG 3 cut(s) 189, 578, 759
BseXI GCAGC 3 cut(s) 446, 509, 1133
BseYI CCCAGC 2 cut(s) 591, 671
Bsh1236I CGCG 6 cut(s) 363, 452, 949, 1096, 1151, 1171
Bsh1285I CGRYCG 3 cut(s) 192, 581, 762
BshFI GGCC 7 cut(s) 98, 191, 331, 517, 580, 761, 1114
BshNI GGYRCC 1 cut(s) 652
BsiEI CGRYCG 3 cut(s) 192, 581, 762
BsiHKAI GWGCWC 1 cut(s) 889
BsiHKCI CYCGRG 5 cut(s) 303, 410, 562, 994, 1002
BslFI GGGAC 5 cut(s) 759, 779, 977, 985, 1020
BslI CCNNNNNNNGG 8 cut(s) 366, 411, 416, 438, 569, 734, 844, 907
BsmAI GTCTC 2 cut(s) 137, 459
BsmBI CGTCTC 1 cut(s) 459
BsmFI GGGAC 5 cut(s) 759, 779, 977, 985, 1020
BsnI GGCC 7 cut(s) 98, 191, 331, 517, 580, 761, 1114
Bso31I GGTCTC 1 cut(s) 137
BsoBI CYCGRG 5 cut(s) 303, 410, 562, 994, 1002
Bsp1286I GDGCHC 3 cut(s) 361, 525, 889
Bsp143I GATC 6 cut(s) 23, 193, 369, 508, 895, 957
BspANI GGCC 7 cut(s) 98, 191, 331, 517, 580, 761, 1114
BspCNI CTCAG 1 cut(s) 242
BspFNI CGCG 6 cut(s) 363, 452, 949, 1096, 1151, 1171
BspPI GGATC 3 cut(s) 188, 377, 952
BspQI GCTCTTC 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 652
BspTNI GGTCTC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 299
BsrFI RCCGGY 2 cut(s) 187, 576
BssAI RCCGGY 2 cut(s) 187, 576
BssMI GATC 6 cut(s) 23, 193, 369, 508, 895, 957
BssNI GRCGYC 2 cut(s) 225, 653
Bst2UI CCWGG 3 cut(s) 534, 545, 614
Bst4CI ACNGT 2 cut(s) 294, 975
Bst6I CTCTTC 2 cut(s) 207, 683
BstACI GRCGYC 2 cut(s) 225, 653
BstBAI YACGTR 1 cut(s) 749
BstC8I GCNNGC 8 cut(s) 189, 361, 495, 578, 621, 696, 928, 1098
BstDEI CTNAG 1 cut(s) 229
BstEII GGTNACC 1 cut(s) 547
BstF5I GGATG 5 cut(s) 274, 397, 578, 958, 1173
BstFNI CGCG 6 cut(s) 363, 452, 949, 1096, 1151, 1171
BstH2I RGCGCY 2 cut(s) 656, 1089
BstHHI GCGC 8 cut(s) 655, 700, 805, 949, 1088, 1151, 1165, 1173
BstKTI GATC 6 cut(s) 26, 196, 372, 511, 898, 960
BstMAI GTCTC 2 cut(s) 137, 459
BstMBI GATC 6 cut(s) 23, 193, 369, 508, 895, 957
BstMCI CGRYCG 3 cut(s) 192, 581, 762
BstMWI GCNNNNNNNGC 6 cut(s) 95, 104, 143, 272, 695, 953
BstNI CCWGG 3 cut(s) 534, 545, 614
BstNSI RCATGY 2 cut(s) 638, 734
BstPAI GACNNNNGTC 1 cut(s) 771
BstPI GGTNACC 1 cut(s) 547
BstSFI CTRYAG 1 cut(s) 1122
BstSLI GKGCMC 1 cut(s) 525
BstUI CGCG 6 cut(s) 363, 452, 949, 1096, 1151, 1171
BstV1I GCAGC 3 cut(s) 446, 509, 1133
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BstZI CGGCCG 3 cut(s) 189, 578, 759
BsuRI GGCC 7 cut(s) 98, 191, 331, 517, 580, 761, 1114
BtsCI GGATG 5 cut(s) 274, 397, 578, 958, 1173
BtsIMutI CAGTG 1 cut(s) 971
Cac8I GCNNGC 8 cut(s) 189, 361, 495, 578, 621, 696, 928, 1098
CfoI GCGC 8 cut(s) 655, 700, 805, 949, 1088, 1151, 1165, 1173
Cfr10I RCCGGY 2 cut(s) 187, 576
Cfr9I CCCGGG 3 cut(s) 410, 994, 1002
CpoI CGGWCCG 1 cut(s) 419
CseI GACGC 2 cut(s) 233, 441
Csp6I GTAC 1 cut(s) 537
CspI CGGWCCG 1 cut(s) 419
CviAII CATG 5 cut(s) 283, 438, 635, 731, 799
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 1 cut(s) 229
DinI GGCGCC 1 cut(s) 654
DpnI GATC 6 cut(s) 25, 195, 371, 510, 897, 959
DpnII GATC 6 cut(s) 23, 193, 369, 508, 895, 957
DraI TTTAAA 1 cut(s) 1051
EaeI YGGCCR 3 cut(s) 189, 578, 759
EagI CGGCCG 3 cut(s) 189, 578, 759
Eam1104I CTCTTC 2 cut(s) 207, 683
EarI CTCTTC 2 cut(s) 207, 683
EciI GGCGGA 1 cut(s) 995
Ecl136II GAGCTC 1 cut(s) 887
EclXI CGGCCG 3 cut(s) 189, 578, 759
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 2 cut(s) 361, 889
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 8 cut(s) 77, 322, 419, 530, 766, 773, 819, 999
Eco52I CGGCCG 3 cut(s) 189, 578, 759
Eco53kI GAGCTC 1 cut(s) 887
Eco72I CACGTG 1 cut(s) 749
Eco88I CYCGRG 5 cut(s) 303, 410, 562, 994, 1002
Eco91I GGTNACC 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 887
EcoO109I RGGNCCY 4 cut(s) 530, 766, 999, 1113
EcoO65I GGTNACC 1 cut(s) 547
EcoRII CCWGG 3 cut(s) 532, 543, 612
EcoT38I GRGCYC 2 cut(s) 361, 889
EgeI GGCGCC 1 cut(s) 654
EheI GGCGCC 1 cut(s) 654
Esp3I CGTCTC 1 cut(s) 459
FaeI CATG 5 cut(s) 286, 441, 638, 734, 802
FalI AAGNNNNNCTT 2 cut(s) 678, 710
FaqI GGGAC 5 cut(s) 759, 779, 977, 985, 1020
FatI CATG 5 cut(s) 282, 437, 634, 730, 798
FauI CCCGC 3 cut(s) 368, 486, 942
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 4 cut(s) 273, 435, 498, 1147
FokI GGATG 5 cut(s) 281, 404, 585, 965, 1160
FriOI GRGCYC 2 cut(s) 361, 889
Fsp4HI GCNGC 4 cut(s) 273, 435, 498, 1147
FspBI CTAG 4 cut(s) 21, 116, 338, 869
GlaI GCGC 8 cut(s) 654, 699, 804, 948, 1087, 1150, 1164, 1172
GluI GCNGC 4 cut(s) 273, 435, 498, 1147
GsaI CCCAGC 2 cut(s) 595, 675
HaeII RGCGCY 2 cut(s) 656, 1089
HaeIII GGCC 7 cut(s) 98, 191, 331, 517, 580, 761, 1114
HgaI GACGC 2 cut(s) 233, 441
HhaI GCGC 8 cut(s) 655, 700, 805, 949, 1088, 1151, 1165, 1173
Hin1I GRCGYC 2 cut(s) 225, 653
Hin1II CATG 5 cut(s) 286, 441, 638, 734, 802
Hin6I GCGC 8 cut(s) 653, 698, 803, 947, 1086, 1149, 1163, 1171
HinP1I GCGC 8 cut(s) 653, 698, 803, 947, 1086, 1149, 1163, 1171
HindIII AAGCTT 1 cut(s) 687
HinfI GANTC 3 cut(s) 173, 917, 1008
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 1 cut(s) 971
Hpy188I TCNGA 6 cut(s) 178, 232, 391, 508, 705, 1017
Hpy188III TCNNGA 2 cut(s) 15, 682
Hpy8I GTNNAC 1 cut(s) 971
Hpy99I CGWCG 2 cut(s) 227, 462
HpyAV CCTTC 3 cut(s) 112, 151, 371
HpyCH4III ACNGT 2 cut(s) 294, 975
HpyCH4IV ACGT 2 cut(s) 748, 1080
HpyCH4V TGCA 3 cut(s) 734, 746, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 104, 143, 272, 695, 953
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 2 cut(s) 748, 1080
Hsp92I GRCGYC 2 cut(s) 225, 653
Hsp92II CATG 5 cut(s) 286, 441, 638, 734, 802
HspAI GCGC 8 cut(s) 653, 698, 803, 947, 1086, 1149, 1163, 1171
KasI GGCGCC 1 cut(s) 652
KflI GGGWCCC 2 cut(s) 766, 999
KroI GCCGGC 2 cut(s) 187, 576
KroNI GCCGGC 2 cut(s) 189, 578
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 6 cut(s) 23, 193, 369, 508, 895, 957
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 6 cut(s) 17, 291, 304, 588, 595, 892
Lsp1109I GCAGC 3 cut(s) 446, 509, 1133
LweI GCATC 5 cut(s) 235, 259, 563, 943, 1182
MaeI CTAG 4 cut(s) 21, 116, 338, 869
MaeII ACGT 2 cut(s) 748, 1080
MaeIII GTNAC 1 cut(s) 547
MalI GATC 6 cut(s) 25, 195, 371, 510, 897, 959
MbiI CCGCTC 1 cut(s) 299
MboI GATC 6 cut(s) 23, 193, 369, 508, 895, 957
MboII GAAGA 3 cut(s) 224, 670, 847
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 3 cut(s) 361, 525, 889
MluCI AATT 4 cut(s) 48, 63, 72, 781
Mly113I GGCGCC 1 cut(s) 653
MlyI GAGTC 1 cut(s) 1002
MnlI CCTC 8 cut(s) 64, 109, 238, 299, 313, 418, 686, 711
MroNI GCCGGC 2 cut(s) 187, 576
MseI TTAA 2 cut(s) 963, 1050
MvaI CCWGG 3 cut(s) 534, 545, 614
MvnI CGCG 6 cut(s) 363, 452, 949, 1096, 1151, 1171
MwoI GCNNNNNNNGC 6 cut(s) 95, 104, 143, 272, 695, 953
NaeI GCCGGC 2 cut(s) 189, 578
NarI GGCGCC 1 cut(s) 653
NdeII GATC 6 cut(s) 23, 193, 369, 508, 895, 957
NgoMIV GCCGGC 2 cut(s) 187, 576
NlaIII CATG 5 cut(s) 286, 441, 638, 734, 802
NmeAIII GCCGAG 3 cut(s) 103, 144, 606
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 2 cut(s) 638, 734
PceI AGGCCT 1 cut(s) 98
PciI ACATGT 1 cut(s) 634
PciSI GCTCTTC 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdiI GCCGGC 2 cut(s) 189, 578
PfeI GAWTC 2 cut(s) 173, 917
PkrI GCNGC 4 cut(s) 274, 436, 499, 1148
PleI GAGTC 1 cut(s) 1002
PluTI GGCGCC 1 cut(s) 656
PmaCI CACGTG 1 cut(s) 749
PmlI CACGTG 1 cut(s) 749
PpsI GAGTC 1 cut(s) 1002
Ppu21I YACGTR 1 cut(s) 749
PpuMI RGGWCCY 3 cut(s) 530, 766, 999
PscI ACATGT 1 cut(s) 634
PshAI GACNNNNGTC 1 cut(s) 771
Psp124BI GAGCTC 1 cut(s) 889
Psp5II RGGWCCY 3 cut(s) 530, 766, 999
Psp6I CCWGG 3 cut(s) 532, 543, 612
PspCI CACGTG 1 cut(s) 749
PspEI GGTNACC 1 cut(s) 547
PspFI CCCAGC 2 cut(s) 591, 671
PspGI CCWGG 3 cut(s) 532, 543, 612
PspPPI RGGWCCY 3 cut(s) 530, 766, 999
PsuI RGATCY 1 cut(s) 369
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
Rsr2I CGGWCCG 1 cut(s) 419
RsrII CGGWCCG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 889
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 2 cut(s) 963, 1050
SatI GCNGC 4 cut(s) 273, 435, 498, 1147
Sau3AI GATC 6 cut(s) 23, 193, 369, 508, 895, 957
SchI GAGTC 1 cut(s) 1002
SduI GDGCHC 3 cut(s) 361, 525, 889
SfaNI GCATC 5 cut(s) 235, 259, 563, 943, 1182
SfcI CTRYAG 1 cut(s) 1122
SfoI GGCGCC 1 cut(s) 654
SinI GGWCC 8 cut(s) 77, 322, 419, 530, 766, 773, 819, 999
SmaI CCCGGG 3 cut(s) 412, 996, 1004
SpeI ACTAGT 1 cut(s) 115
Sse9I AATT 4 cut(s) 48, 63, 72, 781
SseBI AGGCCT 1 cut(s) 98
SspDI GGCGCC 1 cut(s) 652
SspI AATATT 1 cut(s) 1055
SspMI CTAG 4 cut(s) 21, 116, 338, 869
SstI GAGCTC 1 cut(s) 889
StuI AGGCCT 1 cut(s) 98
TaaI ACNGT 2 cut(s) 294, 975
TaiI ACGT 2 cut(s) 751, 1083
TaqI TCGA 3 cut(s) 171, 785, 883
TasI AATT 4 cut(s) 48, 63, 72, 781
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 2 cut(s) 173, 917
Tru1I TTAA 2 cut(s) 963, 1050
Tru9I TTAA 2 cut(s) 963, 1050
TscAI CASTG 1 cut(s) 978
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 3 cut(s) 434, 497, 1146
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 4 cut(s) 51, 225, 475, 847
TspGWI ACGGA 4 cut(s) 432, 734, 879, 1166
TspMI CCCGGG 3 cut(s) 410, 994, 1002
TspRI CASTG 1 cut(s) 978
VpaK11BI GGWCC 8 cut(s) 77, 322, 419, 530, 766, 773, 819, 999
XceI RCATGY 2 cut(s) 638, 734
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmaI CCCGGG 3 cut(s) 410, 994, 1002
XspI CTAG 4 cut(s) 21, 116, 338, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.