RLG00000001028

Belongs to the complex I 30 kDa subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017939
Physical Location & Seq
Forward (+)
9432432 .. 9432941
510 bp
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UTR
Exon/CDS
Intron
RLM00000001028

Sequence Viewer

Length: 510 bp
ATGGAAAGATCGGAACATGGGAATAGATCTGATACCAATACAGACTACCCATTTCAATTATTGTGCTTTCTTAAATTGCATACCTATACAAGGGTTCAAGTTTTGATCAATATTTGCGGAGTTGATTATCCTTCTCGAAAACGAAGATTTGAAGTGGTCTATAGTTTACTGAGTACTCGCTATAACTCACGCATTCGTGTACAAACCAGTACAGACGAAGTAACACGAATATCTCCCATAGTCAGTCTATTTCCATCAACCGGTTGGTGGGAGCGAGAAGTTTGGGATATGTTTGGTGTTTCTTCCATCAATCATCCGGATCTACGCCGCATATCAACAGATTATGGTTTCGAGGGTCATCCATTACGAAAAGATCTTCCTCTGAGTGGATATGTGGAAGTACGGTATGATGATCCAGAAAAACGTGTGGTTTCTGAACCCATTGAGATGACCCAAGAATTTCGCTATTTCGATTTTGCTAGTCCTTGGGAACAGCGTAGCGACGGATAA

Protein Analysis

170

Amino Acids

20.04

Weight (kDa)

5.83

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_30kDa PF00329 19 - 131 1.5e-36 Respiratory-chain NADH dehydrogenase, 30 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017939)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00070
prunus_persica Prupe.2G039100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257991 RchiOBHm_MTg0498881
rosa_laevigata RLG00000000138 RLG00000000143 RLG00000000153 RLG00000000193 RLG00000000489 RLG00000001028
rosa_samantha Rh1BG017400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 2 cut(s) 117, 328
AclWI GGATC 2 cut(s) 327, 407
AcsI RAATTY 1 cut(s) 458
AfaI GTAC 4 cut(s) 175, 201, 211, 402
AfiI CCNNNNNNNGG 4 cut(s) 90, 260, 267, 386
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 3 cut(s) 56, 98, 152
AlwI GGATC 2 cut(s) 327, 407
Aor13HI TCCGGA 1 cut(s) 316
ApoI RAATTY 1 cut(s) 458
AsiGI ACCGGT 1 cut(s) 260
BccI CCATC 2 cut(s) 262, 314
BclI TGATCA 1 cut(s) 105
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 160
BglII AGATCT 2 cut(s) 26, 373
BisI GCNGC 1 cut(s) 328
BlsI GCNGC 1 cut(s) 329
BmcAI AGTACT 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 485
BsaWI WCCGGW 2 cut(s) 260, 316
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 260, 267, 386
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 207
BseAI TCCGGA 1 cut(s) 316
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 2 cut(s) 313, 358
BseLI CCNNNNNNNGG 4 cut(s) 90, 260, 267, 386
BseMII CTCAG 2 cut(s) 161, 374
BseNI ACTGG 1 cut(s) 207
BshTI ACCGGT 1 cut(s) 260
BsiSI CCGG 2 cut(s) 261, 317
BslI CCNNNNNNNGG 4 cut(s) 90, 260, 267, 386
BsmI GAATGC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 316
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 6 cut(s) 8, 26, 105, 319, 373, 412
BspACI CCGC 2 cut(s) 117, 328
BspCNI CTCAG 2 cut(s) 162, 375
BspEI TCCGGA 1 cut(s) 316
BspPI GGATC 2 cut(s) 327, 407
BsrFI RCCGGY 1 cut(s) 260
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 1 cut(s) 207
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 6 cut(s) 8, 26, 105, 319, 373, 412
BssT1I CCWWGG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 405
BstAUI TGTACA 1 cut(s) 199
BstDEI CTNAG 2 cut(s) 170, 383
BstENI CCTNNNNNAGG 1 cut(s) 88
BstF5I GGATG 2 cut(s) 313, 358
BstKTI GATC 6 cut(s) 11, 29, 108, 322, 376, 415
BstMBI GATC 6 cut(s) 8, 26, 105, 319, 373, 412
BstSFI CTRYAG 1 cut(s) 160
BstX2I RGATCY 3 cut(s) 26, 319, 373
BstYI RGATCY 3 cut(s) 26, 319, 373
BtsCI GGATG 2 cut(s) 313, 358
Cfr10I RCCGGY 1 cut(s) 260
Csp6I GTAC 4 cut(s) 174, 200, 210, 401
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 1 cut(s) 17
CviQI GTAC 4 cut(s) 174, 200, 210, 401
DdeI CTNAG 2 cut(s) 170, 383
DpnI GATC 6 cut(s) 10, 28, 107, 321, 375, 414
DpnII GATC 6 cut(s) 8, 26, 105, 319, 373, 412
Eco130I CCWWGG 1 cut(s) 485
EcoNI CCTNNNNNAGG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 1 cut(s) 20
FatI CATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 328
FokI GGATG 2 cut(s) 300, 345
Fsp4HI GCNGC 1 cut(s) 328
FspBI CTAG 1 cut(s) 480
GluI GCNGC 1 cut(s) 328
HapII CCGG 2 cut(s) 261, 317
Hin1II CATG 1 cut(s) 20
HpaII CCGG 2 cut(s) 261, 317
Hpy166II GTNNAC 2 cut(s) 167, 200
Hpy188I TCNGA 4 cut(s) 13, 31, 384, 436
Hpy188III TCNNGA 3 cut(s) 135, 317, 416
Hpy8I GTNNAC 2 cut(s) 167, 200
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 1 cut(s) 141
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4IV ACGT 1 cut(s) 424
HpyCH4V TGCA 1 cut(s) 79
HpyF3I CTNAG 2 cut(s) 170, 383
HpySE526I ACGT 1 cut(s) 424
Hsp92II CATG 1 cut(s) 20
Kpn2I TCCGGA 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 6 cut(s) 8, 26, 105, 319, 373, 412
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 4 cut(s) 220, 274, 330, 429
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 424
MaeIII GTNAC 1 cut(s) 220
MalI GATC 6 cut(s) 10, 28, 107, 321, 375, 414
MboI GATC 6 cut(s) 8, 26, 105, 319, 373, 412
MboII GAAGA 3 cut(s) 156, 294, 368
MflI RGATCY 3 cut(s) 26, 319, 373
MluCI AATT 3 cut(s) 56, 74, 458
MnlI CCTC 2 cut(s) 346, 390
MroI TCCGGA 1 cut(s) 316
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 446
MspI CCGG 2 cut(s) 261, 317
Mva1269I GAATGC 1 cut(s) 192
NdeII GATC 6 cut(s) 8, 26, 105, 319, 373, 412
NlaIII CATG 1 cut(s) 20
PctI GAATGC 1 cut(s) 192
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 1 cut(s) 329
PsuI RGATCY 3 cut(s) 26, 319, 373
RsaI GTAC 4 cut(s) 175, 201, 211, 402
RsaNI GTAC 4 cut(s) 174, 200, 210, 401
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 1 cut(s) 328
Sau3AI GATC 6 cut(s) 8, 26, 105, 319, 373, 412
ScaI AGTACT 1 cut(s) 175
SetI ASST 2 cut(s) 86, 427
SfcI CTRYAG 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 3 cut(s) 56, 74, 458
SsiI CCGC 2 cut(s) 117, 328
SspI AATATT 1 cut(s) 112
SspMI CTAG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 405
TaiI ACGT 1 cut(s) 427
TaqI TCGA 3 cut(s) 136, 351, 471
TasI AATT 3 cut(s) 56, 74, 458
TatI WGTACW 3 cut(s) 173, 199, 209
TauI GCSGC 1 cut(s) 330
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
XagI CCTNNNNNAGG 1 cut(s) 88
XapI RAATTY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 261
XspI CTAG 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.