RLG00000001496

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0022855
Physical Location & Seq
Reverse (-)
16380602 .. 16383649
3048 bp
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UTR
Exon/CDS
Intron
RLM00000001496

Sequence Viewer

Length: 648 bp
ATGATCCTTAATTCCCGCTGGAGAAAAGAACACATTGTAGGAATTAGGATTATCAAGCGGTGGTGGTACACACCAAAAAGATGGTATCCATTCGATTCGTTGATGTGGCTCTTACCTAAACTAGTTTTTCAACTAAATGGACAAGCTCCAATATCTTCTACTGACAAGCCACTAAACCCTCAAGTACTATCTAATGGTTTTGATGTTGCTGAATTCACACCTCCAAGGCAATTAAGGACATATGAAATCCCACAAATTGTCAATGACTTTAGACTTGCTGCAAGGAATGCAATCGAAGCTGGCTTTGATGGGGTTGAAATTCATGGAGCTAATGGTTACCTTATTGATCAGTTTCTGAAAGACCAAGTGAATGATCGAACAGACCAATATGGTGGATCTCTAGAGAATCGCTGCCGATTTGCTCTGGAAACCATTGAAGCTGTTATTGAAATGGGAGCAGATAAAGTTGGAATTACATTATCTCCATTCGTGGACTATATGGAATCAGGGGATTCAAACCCTGAAGCATTAGGCCTTTATATGGTTCAATCATTGAACAAATATGGGCTTCTGTACTGCCGTGTAATTGAGCCAAGAATGAAGGAAGCTGGAGTAAAAAGTGAAAATGCTGATGTCTTCTACCCATGA

Protein Analysis

216

Amino Acids

24.67

Weight (kDa)

5.52

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 72 - 188 2.7e-38 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022855)

Species Orthologous Gene IDs
rosa_laevigata RLG00000001496
rosa_multiflora Rmu_sc0002541.1_g000028 Rmu_sc0007103.1_g000015
rosa_roxburghii Rroxscaffold_3G00230170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 16, 58
AclWI GGATC 1 cut(s) 403
AcsI RAATTY 2 cut(s) 212, 318
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 3 cut(s) 68, 186, 575
AfiI CCNNNNNNNGG 1 cut(s) 541
AgsI TTSAA 7 cut(s) 131, 317, 437, 449, 516, 548, 556
AhlI ACTAGT 1 cut(s) 121
AluBI AGCT 5 cut(s) 146, 299, 329, 440, 608
AluI AGCT 5 cut(s) 146, 299, 329, 440, 608
AlwI GGATC 1 cut(s) 403
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 1 cut(s) 532
ApeKI GCWGC 2 cut(s) 278, 411
ApoI RAATTY 2 cut(s) 212, 318
BbsI GAAGAC 1 cut(s) 628
BbvI GCAGC 2 cut(s) 265, 398
BccI CCATC 2 cut(s) 75, 302
BceAI ACGGC 1 cut(s) 564
BciVI GTATCC 1 cut(s) 96
BclI TGATCA 1 cut(s) 346
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 2 cut(s) 122, 401
BfuI GTATCC 1 cut(s) 96
BisI GCNGC 2 cut(s) 279, 412
BlsI GCNGC 2 cut(s) 280, 413
BmcAI AGTACT 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 628
BpmI CTGGAG 2 cut(s) 40, 630
BpuEI CTTGAG 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 466, 496
Bsc4I CCNNNNNNNGG 1 cut(s) 541
BseDI CCNNGG 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 541
BseXI GCAGC 2 cut(s) 265, 398
BshFI GGCC 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 541
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 1 cut(s) 534
Bsp143I GATC 4 cut(s) 3, 346, 373, 395
BspACI CCGC 2 cut(s) 16, 58
BspANI GGCC 1 cut(s) 534
BspPI GGATC 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 4 cut(s) 3, 346, 373, 395
BssT1I CCWWGG 1 cut(s) 224
BstAPI GCANNNNNTGC 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 301
BstEII GGTNACC 1 cut(s) 335
BstKTI GATC 4 cut(s) 6, 349, 376, 398
BstMBI GATC 4 cut(s) 3, 346, 373, 395
BstMWI GCNNNNNNNGC 2 cut(s) 287, 296
BstPI GGTNACC 1 cut(s) 335
BstV1I GCAGC 2 cut(s) 265, 398
BstV2I GAAGAC 1 cut(s) 628
BstX2I RGATCY 1 cut(s) 395
BstXI CCANNNNNNTGG 2 cut(s) 81, 392
BstYI RGATCY 1 cut(s) 395
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 355
Csp6I GTAC 3 cut(s) 67, 185, 574
CviAII CATG 2 cut(s) 323, 645
CviQI GTAC 3 cut(s) 67, 185, 574
DpnI GATC 4 cut(s) 5, 348, 375, 397
DpnII GATC 4 cut(s) 3, 346, 373, 395
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 543
Eco91I GGTNACC 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 2 cut(s) 326, 648
FatI CATG 2 cut(s) 322, 644
FauI CCCGC 1 cut(s) 23
FauNDI CATATG 1 cut(s) 241
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 2 cut(s) 279, 412
Fsp4HI GCNGC 2 cut(s) 279, 412
FspBI CTAG 2 cut(s) 122, 401
GluI GCNGC 2 cut(s) 279, 412
GsuI CTGGAG 2 cut(s) 40, 630
HaeIII GGCC 1 cut(s) 534
Hin1II CATG 2 cut(s) 326, 648
HinfI GANTC 4 cut(s) 95, 406, 503, 512
Hpy166II GTNNAC 2 cut(s) 69, 493
Hpy188I TCNGA 1 cut(s) 357
Hpy188III TCNNGA 2 cut(s) 401, 425
Hpy8I GTNNAC 2 cut(s) 69, 493
HpyAV CCTTC 1 cut(s) 595
HpyCH4V TGCA 2 cut(s) 281, 290
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 296
Hsp92II CATG 2 cut(s) 326, 648
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 4 cut(s) 3, 346, 373, 395
LmnI GCTCC 3 cut(s) 151, 326, 455
LpnPI CCDG 6 cut(s) 4, 285, 410, 492, 534, 594
Lsp1109I GCAGC 2 cut(s) 265, 398
MaeI CTAG 2 cut(s) 122, 401
MaeIII GTNAC 1 cut(s) 335
MalI GATC 4 cut(s) 5, 348, 375, 397
MboI GATC 4 cut(s) 3, 346, 373, 395
MboII GAAGA 2 cut(s) 147, 628
MflI RGATCY 1 cut(s) 395
MluCI AATT 8 cut(s) 10, 42, 212, 230, 255, 318, 471, 585
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 2 cut(s) 189, 231
MseI TTAA 2 cut(s) 9, 233
MspA1I CMGCKG 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 292
MwoI GCNNNNNNNGC 2 cut(s) 287, 296
NdeI CATATG 1 cut(s) 241
NdeII GATC 4 cut(s) 3, 346, 373, 395
NlaIII CATG 2 cut(s) 326, 648
PceI AGGCCT 1 cut(s) 534
PctI GAATGC 1 cut(s) 292
PfeI GAWTC 4 cut(s) 95, 406, 503, 512
PkrI GCNGC 2 cut(s) 280, 413
PspEI GGTNACC 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 395
RsaI GTAC 3 cut(s) 68, 186, 575
RsaNI GTAC 3 cut(s) 67, 185, 574
SaqAI TTAA 2 cut(s) 9, 233
SatI GCNGC 2 cut(s) 279, 412
Sau3AI GATC 4 cut(s) 3, 346, 373, 395
ScaI AGTACT 1 cut(s) 186
SetI ASST 8 cut(s) 118, 148, 223, 301, 331, 342, 442, 610
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
SpeI ACTAGT 1 cut(s) 121
Sse9I AATT 8 cut(s) 10, 42, 212, 230, 255, 318, 471, 585
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 2 cut(s) 16, 58
SspMI CTAG 2 cut(s) 122, 401
StuI AGGCCT 1 cut(s) 534
StyI CCWWGG 1 cut(s) 224
TaqI TCGA 3 cut(s) 93, 294, 376
TasI AATT 8 cut(s) 10, 42, 212, 230, 255, 318, 471, 585
TatI WGTACW 2 cut(s) 184, 573
TfiI GAWTC 4 cut(s) 95, 406, 503, 512
Tru1I TTAA 2 cut(s) 9, 233
Tru9I TTAA 2 cut(s) 9, 233
TseI GCWGC 2 cut(s) 278, 411
TspDTI ATGAA 3 cut(s) 258, 311, 614
XapI RAATTY 2 cut(s) 212, 318
XbaI TCTAGA 1 cut(s) 400
XspI CTAG 2 cut(s) 122, 401
ZrmI AGTACT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.