Rroxscaffold_3G00230170

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0022855
Physical Location & Seq
Reverse (-)
14767163 .. 14768546
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00230170.1

Sequence Viewer

Length: 504 bp
ATGGATTCCAGTACCCTTGTGATTGGACCAAGAAAAGAGGATGAATTACAGATCAGGGGTTTCACCACCGTTTTTCAACCAAATGGACAAGCTCCAATATCTTCTACTGACAAGCCACTAACCCGTCAAGTACTATCTAATGGTGTTGATGTCGCTGAATTCACACCTCCAAGGCAATTAAGGACATATGAAATCCCACAAATTGTCAATGACTTTAGACTTGCTGCAAGGAATGCAATCGAAGCTGAGAATCGCAGCCGATTTGCTCTGGAAATCATTGAAGCTGTTAATGAAATAGGAGCAGATAAAGTTGGAATTAGATTATCTCCATTTGCGGACTATATGGAATCAGGGGATTCAAAACCTGAAGCATTAGGCCTTTATATTGTTCAGTCCTTGAACAAATATGGGATTCTGTACTGCCATGTGATTGAGCCAAGAATGAAGGAAGCTGGAGTAAAAAGTGAAAATGCTGAAGTCTTCTACCGTGAGAGAAGCTTTTAG

Protein Analysis

167

Amino Acids

18.77

Weight (kDa)

5.17

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 82 - 154 7.4e-09 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022855)

Species Orthologous Gene IDs
rosa_laevigata RLG00000001496
rosa_multiflora Rmu_sc0002541.1_g000028 Rmu_sc0007103.1_g000015
rosa_roxburghii Rroxscaffold_3G00230170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 2 cut(s) 387, 495
AfaI GTAC 3 cut(s) 13, 132, 419
AgsI TTSAA 4 cut(s) 77, 281, 360, 400
AluBI AGCT 5 cut(s) 92, 245, 284, 452, 498
AluI AGCT 5 cut(s) 92, 245, 284, 452, 498
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 2 cut(s) 224, 255
ApoI RAATTY 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 472
BbvI GCAGC 2 cut(s) 211, 267
BisI GCNGC 2 cut(s) 225, 256
BlsI GCNGC 2 cut(s) 226, 257
BmcAI AGTACT 1 cut(s) 132
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 472
BpmI CTGGAG 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 46
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 9
BseXI GCAGC 2 cut(s) 211, 267
BshFI GGCC 1 cut(s) 378
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 378
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 1 cut(s) 335
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 1 cut(s) 238
BsrI ACTGG 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 70, 488
BstAPI GCANNNNNTGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 246
BstF5I GGATG 1 cut(s) 46
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 2 cut(s) 233, 242
BstV1I GCAGC 2 cut(s) 211, 267
BstV2I GAAGAC 1 cut(s) 472
BsuRI GGCC 1 cut(s) 378
BtsCI GGATG 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 26
Csp6I GTAC 3 cut(s) 12, 131, 418
CviAII CATG 1 cut(s) 425
CviJI RGCY 9 cut(s) 92, 115, 245, 258, 284, 378, 436, 452, 498
CviKI_1 RGCY 9 cut(s) 92, 115, 245, 258, 284, 378, 436, 452, 498
CviQI GTAC 3 cut(s) 12, 131, 418
DdeI CTNAG 1 cut(s) 246
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 170
Eco147I AGGCCT 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 26
Eco57I CTGAAG 2 cut(s) 387, 495
EcoRI GAATTC 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 1 cut(s) 428
FaiI YATR 7 cut(s) 187, 189, 342, 344, 384, 408, 426
FatI CATG 1 cut(s) 424
FauNDI CATATG 1 cut(s) 187
Fnu4HI GCNGC 2 cut(s) 225, 256
FokI GGATG 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 225, 256
GluI GCNGC 2 cut(s) 225, 256
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 1 cut(s) 378
Hin1II CATG 1 cut(s) 428
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 5 cut(s) 5, 250, 347, 356, 412
HphI GGTGA 1 cut(s) 55
Hpy188III TCNNGA 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 439
HpyCH4III ACNGT 2 cut(s) 70, 488
HpyCH4V TGCA 2 cut(s) 227, 236
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 242
HpyF3I CTNAG 1 cut(s) 246
Hsp92II CATG 1 cut(s) 428
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 2 cut(s) 97, 299
LpnPI CCDG 6 cut(s) 22, 40, 254, 336, 378, 438
Lsp1109I GCAGC 2 cut(s) 211, 267
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 2 cut(s) 93, 472
MluCI AATT 5 cut(s) 44, 158, 176, 201, 315
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 2 cut(s) 31, 177
MseI TTAA 2 cut(s) 179, 288
Mva1269I GAATGC 1 cut(s) 238
MwoI GCNNNNNNNGC 2 cut(s) 233, 242
NdeI CATATG 1 cut(s) 187
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 428
PceI AGGCCT 1 cut(s) 378
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 5 cut(s) 5, 250, 347, 356, 412
PkrI GCNGC 2 cut(s) 226, 257
PspPI GGNCC 1 cut(s) 26
RsaI GTAC 3 cut(s) 13, 132, 419
RsaNI GTAC 3 cut(s) 12, 131, 418
SaqAI TTAA 2 cut(s) 179, 288
SatI GCNGC 2 cut(s) 225, 256
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 26
ScaI AGTACT 1 cut(s) 132
SetI ASST 7 cut(s) 94, 169, 247, 286, 367, 454, 500
SinI GGWCC 1 cut(s) 26
Sse9I AATT 5 cut(s) 44, 158, 176, 201, 315
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 1 cut(s) 335
StuI AGGCCT 1 cut(s) 378
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 2 cut(s) 70, 488
TaqI TCGA 1 cut(s) 240
TasI AATT 5 cut(s) 44, 158, 176, 201, 315
TatI WGTACW 2 cut(s) 130, 417
TfiI GAWTC 5 cut(s) 5, 250, 347, 356, 412
Tru1I TTAA 2 cut(s) 179, 288
Tru9I TTAA 2 cut(s) 179, 288
TseI GCWGC 2 cut(s) 224, 255
TspDTI ATGAA 4 cut(s) 57, 204, 306, 458
VpaK11BI GGWCC 1 cut(s) 26
XapI RAATTY 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.