RLG00000003306

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0022840
Physical Location & Seq
Reverse (-)
46951406 .. 46952780
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003306

Sequence Viewer

Length: 357 bp
ATGAGACCGGCACGTCTACTTGGCCAAGCTCACCGAGCAGGCGGAGCGCTACGAGGAGATAGGTCCACCACGACGTCGGAGCTCACCGTCGAGGAGCGCATCGAGCAGAAGGAGGAGGGCCGCAAGAACAAGAAACTGTTCTTATCGTTGACAATGAAAAAGCAGATGAGGAGGAGGAGAAGGAAGAAGAGCTCGCCAGCAGATCCGAAGCCGGCCGCTCCCCCCAAAGATGACGCCGAGCAGAAGGCAGTGGCCGCGCTGACGGAGGCCTTCTCTTCAGTCTCAGTCGACGACGTCATTTCGGCGCTTCGCGAAGCCAGAGGCCACCCGGACAAGGCCGCCGAGATTTTGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

119

Amino Acids

13.04

Weight (kDa)

10.26

Isoelectric Point (pI)

67.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_At5g58720 PF24767 78 - 117 2.1e-16 At5g58720 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022840)

Species Orthologous Gene IDs
rosa_laevigata RLG00000003306
rosa_multiflora Rmu_sc0003551.1_g000002
rosa_samantha Rh5CG141400 Rh6AG119800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 12
AatII GACGTC 2 cut(s) 77, 297
AccBSI CCGCTC 1 cut(s) 218
AccI GTMKAC 2 cut(s) 16, 288
AccII CGCG 2 cut(s) 257, 312
AciI CCGC 5 cut(s) 42, 121, 216, 255, 339
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 3 cut(s) 22, 213, 252
AcuI CTGAAG 1 cut(s) 261
AcyI GRCGYC 3 cut(s) 74, 234, 294
AfeI AGCGCT 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 334
AjiI CACGTC 1 cut(s) 14
AluBI AGCT 3 cut(s) 29, 82, 192
AluI AGCT 3 cut(s) 29, 82, 192
Alw21I GWGCWC 2 cut(s) 84, 194
Alw26I GTCTC 1 cut(s) 286
AlwI GGATC 1 cut(s) 197
Aor51HI AGCGCT 1 cut(s) 48
AoxI GGCC 7 cut(s) 22, 118, 213, 252, 267, 322, 336
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 4 cut(s) 49, 99, 259, 307
AspS9I GGNCC 2 cut(s) 63, 118
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 2 cut(s) 23, 76
AvaII GGWCC 1 cut(s) 63
BalI TGGCCA 1 cut(s) 24
BanII GRGCYC 2 cut(s) 84, 194
Bbv12I GWGCWC 2 cut(s) 84, 194
BcnI CCSGG 1 cut(s) 329
BcoDI GTCTC 1 cut(s) 286
BfaI CTAG 1 cut(s) 355
BfoI RGCGCY 2 cut(s) 50, 308
BisI GCNGC 4 cut(s) 121, 216, 255, 339
BlsI GCNGC 4 cut(s) 122, 217, 256, 340
Bme1390I CCNGG 1 cut(s) 329
Bme18I GGWCC 1 cut(s) 63
BmgBI CACGTC 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 63, 118
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 1 cut(s) 108
BplI GAGNNNNNCTC 2 cut(s) 257, 289
BpuMI CCSGG 1 cut(s) 329
BsaHI GRCGYC 3 cut(s) 74, 234, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse118I RCCGGY 2 cut(s) 7, 211
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMII CTCAG 1 cut(s) 297
BseRI GAGGAG 6 cut(s) 69, 107, 128, 184, 187, 190
BseX3I CGGCCG 1 cut(s) 213
Bsh1236I CGCG 2 cut(s) 257, 312
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 7 cut(s) 24, 120, 215, 254, 269, 324, 338
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 84, 194
BsiSI CCGG 3 cut(s) 8, 212, 329
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 286
BsnI GGCC 7 cut(s) 24, 120, 215, 254, 269, 324, 338
Bsp1286I GDGCHC 2 cut(s) 84, 194
Bsp143I GATC 1 cut(s) 202
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 5 cut(s) 42, 121, 216, 255, 339
BspANI GGCC 7 cut(s) 24, 120, 215, 254, 269, 324, 338
BspCNI CTCAG 1 cut(s) 296
BspFNI CGCG 2 cut(s) 257, 312
BspPI GGATC 1 cut(s) 197
BspQI GCTCTTC 1 cut(s) 182
BsrBI CCGCTC 1 cut(s) 218
BsrFI RCCGGY 2 cut(s) 7, 211
BssAI RCCGGY 2 cut(s) 7, 211
BssMI GATC 1 cut(s) 202
BssNI GRCGYC 3 cut(s) 74, 234, 294
Bst4CI ACNGT 2 cut(s) 88, 138
Bst6I CTCTTC 2 cut(s) 182, 280
BstACI GRCGYC 3 cut(s) 74, 234, 294
BstC8I GCNNGC 4 cut(s) 40, 194, 198, 213
BstDEI CTNAG 1 cut(s) 283
BstFNI CGCG 2 cut(s) 257, 312
BstH2I RGCGCY 2 cut(s) 50, 308
BstHHI GCGC 4 cut(s) 49, 99, 259, 307
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 1 cut(s) 202
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 4 cut(s) 35, 44, 103, 254
BstSCI CCNGG 1 cut(s) 327
BstUI CGCG 2 cut(s) 257, 312
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BstZI CGGCCG 1 cut(s) 213
BsuRI GGCC 7 cut(s) 24, 120, 215, 254, 269, 324, 338
BtrI CACGTC 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 4 cut(s) 40, 194, 198, 213
CfoI GCGC 4 cut(s) 49, 99, 259, 307
Cfr10I RCCGGY 2 cut(s) 7, 211
Cfr13I GGNCC 2 cut(s) 63, 118
CseI GACGC 1 cut(s) 242
DdeI CTNAG 1 cut(s) 283
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
DrdI GACNNNNNNGTC 1 cut(s) 12
DseDI GACNNNNNNGTC 1 cut(s) 12
EaeI YGGCCR 3 cut(s) 22, 213, 252
EagI CGGCCG 1 cut(s) 213
Eam1104I CTCTTC 2 cut(s) 182, 280
EarI CTCTTC 2 cut(s) 182, 280
EciI GGCGGA 1 cut(s) 57
Ecl136II GAGCTC 2 cut(s) 82, 192
EclXI CGGCCG 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 269
Eco24I GRGCYC 2 cut(s) 84, 194
Eco47I GGWCC 1 cut(s) 63
Eco47III AGCGCT 1 cut(s) 48
Eco52I CGGCCG 1 cut(s) 213
Eco53kI GAGCTC 2 cut(s) 82, 192
Eco57I CTGAAG 1 cut(s) 261
EcoICRI GAGCTC 2 cut(s) 82, 192
EcoT38I GRGCYC 2 cut(s) 84, 194
FblI GTMKAC 2 cut(s) 16, 288
Fnu4HI GCNGC 4 cut(s) 121, 216, 255, 339
FriOI GRGCYC 2 cut(s) 84, 194
Fsp4HI GCNGC 4 cut(s) 121, 216, 255, 339
FspBI CTAG 1 cut(s) 355
GlaI GCGC 4 cut(s) 48, 98, 258, 306
GluI GCNGC 4 cut(s) 121, 216, 255, 339
HaeII RGCGCY 2 cut(s) 50, 308
HaeIII GGCC 7 cut(s) 24, 120, 215, 254, 269, 324, 338
HapII CCGG 3 cut(s) 8, 212, 329
HgaI GACGC 1 cut(s) 242
HhaI GCGC 4 cut(s) 49, 99, 259, 307
Hin1I GRCGYC 3 cut(s) 74, 234, 294
Hin6I GCGC 4 cut(s) 47, 97, 257, 305
HinP1I GCGC 4 cut(s) 47, 97, 257, 305
HincII GTYRAC 2 cut(s) 150, 289
HindII GTYRAC 2 cut(s) 150, 289
HpaII CCGG 3 cut(s) 8, 212, 329
HphI GGTGA 2 cut(s) 23, 76
Hpy166II GTNNAC 4 cut(s) 17, 66, 150, 289
Hpy188I TCNGA 2 cut(s) 79, 207
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 4 cut(s) 17, 66, 150, 289
Hpy99I CGWCG 5 cut(s) 76, 79, 92, 293, 296
HpyAV CCTTC 4 cut(s) 103, 174, 238, 280
HpyCH4III ACNGT 2 cut(s) 88, 138
HpyCH4IV ACGT 3 cut(s) 13, 74, 294
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 44, 103, 254
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 3 cut(s) 13, 74, 294
Hsp92I GRCGYC 3 cut(s) 74, 234, 294
HspAI GCGC 4 cut(s) 47, 97, 257, 305
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Kzo9I GATC 1 cut(s) 202
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 4 cut(s) 44, 79, 94, 223
LpnPI CCDG 6 cut(s) 21, 24, 210, 225, 331, 342
LweI GCATC 1 cut(s) 108
MaeI CTAG 1 cut(s) 355
MaeII ACGT 3 cut(s) 13, 74, 294
MalI GATC 1 cut(s) 204
MbiI CCGCTC 1 cut(s) 218
MboI GATC 1 cut(s) 202
MboII GAAGA 3 cut(s) 196, 199, 267
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 84, 194
MlsI TGGCCA 1 cut(s) 24
MluNI TGGCCA 1 cut(s) 24
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 9 cut(s) 47, 85, 106, 109, 162, 165, 168, 259, 314
Mox20I TGGCCA 1 cut(s) 24
MroNI GCCGGC 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 137
MscI TGGCCA 1 cut(s) 24
Msp20I TGGCCA 1 cut(s) 24
MspI CCGG 3 cut(s) 8, 212, 329
MspR9I CCNGG 1 cut(s) 329
MvnI CGCG 2 cut(s) 257, 312
MwoI GCNNNNNNNGC 4 cut(s) 35, 44, 103, 254
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 1 cut(s) 329
NdeII GATC 1 cut(s) 202
NgoMIV GCCGGC 1 cut(s) 211
NmeAIII GCCGAG 1 cut(s) 262
NruI TCGCGA 1 cut(s) 312
PceI AGGCCT 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 137
PflFI GACNNNGTC 1 cut(s) 293
PkrI GCNGC 4 cut(s) 122, 217, 256, 340
Psp124BI GAGCTC 2 cut(s) 84, 194
PspPI GGNCC 2 cut(s) 63, 118
PsuI RGATCY 1 cut(s) 202
PsyI GACNNNGTC 1 cut(s) 293
RruI TCGCGA 1 cut(s) 312
SacI GAGCTC 2 cut(s) 84, 194
SalI GTCGAC 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 182
SatI GCNGC 4 cut(s) 121, 216, 255, 339
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 2 cut(s) 63, 118
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 2 cut(s) 84, 194
SetI ASST 7 cut(s) 16, 31, 65, 77, 84, 194, 297
SfaNI GCATC 1 cut(s) 108
SinI GGWCC 1 cut(s) 63
SseBI AGGCCT 1 cut(s) 269
SsiI CCGC 5 cut(s) 42, 121, 216, 255, 339
SspMI CTAG 1 cut(s) 355
SstI GAGCTC 2 cut(s) 84, 194
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 88, 138
TaiI ACGT 3 cut(s) 16, 77, 297
TaqI TCGA 3 cut(s) 90, 102, 288
TauI GCSGC 4 cut(s) 123, 218, 257, 341
TscAI CASTG 1 cut(s) 255
TspDTI ATGAA 1 cut(s) 170
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 255
Tth111I GACNNNGTC 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 63
XmiI GTMKAC 2 cut(s) 16, 288
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 1 cut(s) 355
ZraI GACGTC 2 cut(s) 75, 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.