RLG00000003738

sugar phosphate phosphate translocator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016055
Physical Location & Seq
Reverse (-)
52192575 .. 52193660
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003738

Sequence Viewer

Length: 1086 bp
ATGGTGGAAGCCCAGACATGGACGACGCGTCGTATGAGCAACCCCCGGCTGGACGCCTCCTCCTCCCCCGACCTCGCCGTCGACATCCCAGCCACTCCCCCCGGCGACGTCCGCAACGGCACTAGCTACAACAACTACGGCTACTCCTTCGGATCGTTCCTCTCCCCGACTCTATTCACGGCCGCCATCATCGCCTCCTGGTACTTGTCCAACATCGGAGTCCTCCTCCTCAACAAATACCTCCTCAGCTACTACGGCTACCGCTACCCTATCTTCCTCACAATGCTCCACATGATCTCCTGCGCCGTCTACAGCTACGTCGCCATCCACTTCCTCGAGCTCGTGCCACGTCAGCACATCGTCTCCCGCCGCCAGTTCTTCAAGATCCTCGCCCTCAGCGCCATCTTCTGCTTCTCCGTCGTCTGCGGCAACACCTCTCTCCGCTACCTCCCCGTCTCCTTCAATCAGGCCATCGGCGCCACCACCCCGTTCTTTACTGCGATTTTCGCTTGCATAATCACCTGTAAGAAGGAATCCGCCGAGGTTTACGGCGCCCTACTCCCCGTAGTTTTCGGAATCGTCCTCGCCAGCAACAGCGAGCCGCTGTTCCATCTTTTCGGGTTTCTGGTCTGCGTCGGGTCGACGGCCGGCCGGGCGTTGAAGTCCGTGGTGCAGGGGATTTTGCTGACTAACGACTCCGAGAAGCTCCACTCGATGAACTTGCTGCTCTACATGGCGCCGATGGCGGCTTGCATTTTGCTGCCGTTTACTCTCTACATCGAGGGCAACGTGGCGGCGAAGACGATAGAGAAGGCGAGGGGAGACCCGTACATTGTGTTCTTGTTGCTCGGGAACGCCACCGTGGCGTACTTGGTCAACTTGACCAATTTCTTGGTGACCAAGCACACAAGCGCGTTGACGCTGCAGGTGCTTGGAAATGCCAAGGCGGCCGTCGCGGCGGTGGTGTCGGTTTTGATATTCCGGAACCCGGTGACGGTGATGGGGATGACCGGGTTCGCGGTGACCATCATGGGCGTGGTGCTTTACAGCGAGGCCAAGAAGAGATCCAAAGTTACAACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

362

Amino Acids

39.43

Weight (kDa)

9.31

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 61 - 350 1.1e-48 Triose-phosphate Transporter family
UAA PF08449 63 - 352 3.6e-10 UAA transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12500
fragaria_vesca FvH4_5g01050
malus_domestica MD04G1075500.v1.1 MD06G1068400.v1.1
prunus_persica Prupe.5G084500_v2.0.a1
pyrus_communis pycom06g06640
rosa_chinensis RchiOBHm_Chr7g0200301
rosa_laevigata RLG00000003738
rosa_multiflora Rmu_sc0005350.1_g000003
rosa_roxburghii Rroxscaffold_3G00256170
rosa_rugosa Rorug07G0059000
rosa_samantha Rh7BG187100 Rh7CG194800 Rh7DG188400
rosa_wichuraiana Rw7G016150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 916
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 111
Acc36I ACCTGC 1 cut(s) 916
AccB1I GGYRCC 3 cut(s) 476, 551, 736
AccI GTMKAC 3 cut(s) 81, 309, 641
AccII CGCG 4 cut(s) 28, 914, 956, 1019
AccIII TCCGGA 1 cut(s) 981
AclWI GGATC 3 cut(s) 160, 379, 1059
AcoI YGGCCR 4 cut(s) 180, 645, 649, 948
AcyI GRCGYC 5 cut(s) 54, 108, 477, 552, 737
AfaI GTAC 3 cut(s) 203, 830, 869
AfiI CCNNNNNNNGG 4 cut(s) 18, 49, 988, 994
AflIII ACRYGT 1 cut(s) 26
AgsI TTSAA 3 cut(s) 382, 463, 661
AhdI GACNNNNNGTC 1 cut(s) 27
AjiI CACGTC 1 cut(s) 350
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 5 cut(s) 126, 249, 315, 340, 706
AluI AGCT 5 cut(s) 126, 249, 315, 340, 706
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 3 cut(s) 367, 460, 816
AlwI GGATC 3 cut(s) 160, 379, 1059
Ama87I CYCGRG 2 cut(s) 335, 848
Aor13HI TCCGGA 1 cut(s) 981
AoxI GGCC 6 cut(s) 180, 468, 645, 649, 948, 1053
ApeKI GCWGC 3 cut(s) 724, 760, 922
AspLEI GCGC 6 cut(s) 305, 401, 479, 554, 739, 914
AsuC2I CCSGG 5 cut(s) 46, 102, 653, 989, 1012
AsuHPI GGTGA 5 cut(s) 511, 907, 1003, 1009, 1033
AvaI CYCGRG 2 cut(s) 335, 848
BanI GGYRCC 3 cut(s) 476, 551, 736
BanII GRGCYC 1 cut(s) 342
BauI CACGAG 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 806
Bbv12I GWGCWC 1 cut(s) 342
BbvCI CCTCAGC 2 cut(s) 245, 395
BbvI GCAGC 3 cut(s) 711, 747, 909
BccI CCATC 8 cut(s) 194, 332, 410, 479, 618, 736, 994, 1034
BcgI CGANNNNNNTGC 2 cut(s) 703, 737
BciT130I CCWGG 1 cut(s) 199
BcnI CCSGG 5 cut(s) 46, 102, 653, 989, 1012
BcoDI GTCTC 3 cut(s) 367, 460, 816
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 2 cut(s) 310, 923
BfoI RGCGCY 4 cut(s) 402, 480, 555, 740
BfuAI ACCTGC 1 cut(s) 916
BglI GCCNNNNNGGC 4 cut(s) 653, 863, 947, 956
Bme1390I CCNGG 6 cut(s) 46, 102, 199, 653, 989, 1012
BmeRI GACNNNNNGTC 1 cut(s) 27
BmeT110I CYCGRG 2 cut(s) 335, 848
BmgBI CACGTC 1 cut(s) 350
BmiI GGNNCC 4 cut(s) 478, 553, 738, 986
BmrFI CCNGG 6 cut(s) 46, 102, 199, 653, 989, 1012
BpiI GAAGAC 1 cut(s) 806
BplI GAGNNNNNCTC 2 cut(s) 210, 242
Bpu10I CCTNAGC 2 cut(s) 245, 395
BpuMI CCSGG 5 cut(s) 46, 102, 653, 989, 1012
BsaHI GRCGYC 5 cut(s) 54, 108, 477, 552, 737
BsaI GGTCTC 1 cut(s) 816
BsaJI CCNNGG 6 cut(s) 44, 100, 540, 666, 861, 942
BsaWI WCCGGW 1 cut(s) 981
BsaXI ACNNNNNCTCC 8 cut(s) 44, 74, 128, 158, 281, 311, 347, 377
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 49, 988, 994
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 1 cut(s) 373
BseAI TCCGGA 1 cut(s) 981
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 6 cut(s) 44, 100, 540, 666, 861, 942
BseGI GGATG 3 cut(s) 84, 324, 1011
BseLI CCNNNNNNNGG 4 cut(s) 18, 49, 988, 994
BseMII CTCAG 2 cut(s) 259, 409
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 5 cut(s) 49, 52, 215, 218, 233
BseX3I CGGCCG 4 cut(s) 180, 645, 649, 948
BseXI GCAGC 3 cut(s) 711, 747, 909
BseYI CCCAGC 1 cut(s) 88
BsgI GTGCAG 1 cut(s) 692
Bsh1236I CGCG 4 cut(s) 28, 914, 956, 1019
Bsh1285I CGRYCG 4 cut(s) 183, 648, 652, 951
BshFI GGCC 6 cut(s) 182, 470, 647, 651, 950, 1055
BshNI GGYRCC 3 cut(s) 476, 551, 736
BsiEI CGRYCG 4 cut(s) 183, 648, 652, 951
BsiHKAI GWGCWC 1 cut(s) 342
BsiHKCI CYCGRG 2 cut(s) 335, 848
BsiSI CCGG 7 cut(s) 46, 102, 648, 652, 982, 989, 1011
BslI CCNNNNNNNGG 4 cut(s) 18, 49, 988, 994
BsmAI GTCTC 3 cut(s) 367, 460, 816
BsmBI CGTCTC 2 cut(s) 367, 460
BsnI GGCC 6 cut(s) 182, 470, 647, 651, 950, 1055
Bso31I GGTCTC 1 cut(s) 816
BsoBI CYCGRG 2 cut(s) 335, 848
Bsp1286I GDGCHC 1 cut(s) 342
Bsp13I TCCGGA 1 cut(s) 981
Bsp143I GATC 4 cut(s) 152, 294, 384, 1064
BspANI GGCC 6 cut(s) 182, 470, 647, 651, 950, 1055
BspCNI CTCAG 2 cut(s) 258, 408
BspEI TCCGGA 1 cut(s) 981
BspFNI CGCG 4 cut(s) 28, 914, 956, 1019
BspLI GGNNCC 4 cut(s) 478, 553, 738, 986
BspMAI CTGCAG 1 cut(s) 927
BspMI ACCTGC 1 cut(s) 916
BspPI GGATC 3 cut(s) 160, 379, 1059
BspT107I GGYRCC 3 cut(s) 476, 551, 736
BspTNI GGTCTC 1 cut(s) 816
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 6 cut(s) 44, 100, 540, 666, 861, 942
BssMI GATC 4 cut(s) 152, 294, 384, 1064
BssNI GRCGYC 5 cut(s) 54, 108, 477, 552, 737
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 942
Bst2BI CACGAG 1 cut(s) 341
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 2 cut(s) 862, 997
Bst6I CTCTTC 1 cut(s) 1055
BstACI GRCGYC 5 cut(s) 54, 108, 477, 552, 737
BstC8I GCNNGC 5 cut(s) 511, 589, 599, 649, 751
BstDEI CTNAG 2 cut(s) 245, 395
BstDSI CCRYGG 2 cut(s) 666, 861
BstEII GGTNACC 2 cut(s) 895, 1021
BstF5I GGATG 3 cut(s) 84, 324, 1011
BstFNI CGCG 4 cut(s) 28, 914, 956, 1019
BstH2I RGCGCY 4 cut(s) 402, 480, 555, 740
BstHHI GCGC 6 cut(s) 305, 401, 479, 554, 739, 914
BstKTI GATC 4 cut(s) 155, 297, 387, 1067
BstMAI GTCTC 3 cut(s) 367, 460, 816
BstMBI GATC 4 cut(s) 152, 294, 384, 1064
BstMCI CGRYCG 4 cut(s) 183, 648, 652, 951
BstNI CCWGG 1 cut(s) 199
BstPI GGTNACC 2 cut(s) 895, 1021
BstSCI CCNGG 6 cut(s) 44, 100, 197, 651, 987, 1010
BstSFI CTRYAG 2 cut(s) 310, 923
BstUI CGCG 4 cut(s) 28, 914, 956, 1019
BstV1I GCAGC 3 cut(s) 711, 747, 909
BstV2I GAAGAC 1 cut(s) 806
BstX2I RGATCY 2 cut(s) 384, 1064
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 2 cut(s) 384, 1064
BstZI CGGCCG 4 cut(s) 180, 645, 649, 948
BsuRI GGCC 6 cut(s) 182, 470, 647, 651, 950, 1055
BtgI CCRYGG 2 cut(s) 666, 861
BtgZI GCGATG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 350
BtsCI GGATG 3 cut(s) 84, 324, 1011
BveI ACCTGC 1 cut(s) 916
Cac8I GCNNGC 5 cut(s) 511, 589, 599, 649, 751
CfoI GCGC 6 cut(s) 305, 401, 479, 554, 739, 914
Cfr10I RCCGGY 1 cut(s) 647
CseI GACGC 5 cut(s) 17, 34, 62, 622, 928
Csp6I GTAC 3 cut(s) 202, 829, 868
CviAII CATG 4 cut(s) 18, 292, 733, 1030
CviQI GTAC 3 cut(s) 202, 829, 868
DdeI CTNAG 2 cut(s) 245, 395
DinI GGCGCC 3 cut(s) 478, 553, 738
DpnI GATC 4 cut(s) 154, 296, 386, 1066
DpnII GATC 4 cut(s) 152, 294, 384, 1064
DrdI GACNNNNNNGTC 1 cut(s) 77
DriI GACNNNNNGTC 1 cut(s) 27
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 4 cut(s) 180, 645, 649, 948
EagI CGGCCG 4 cut(s) 180, 645, 649, 948
Eam1104I CTCTTC 1 cut(s) 1055
Eam1105I GACNNNNNGTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 1055
EciI GGCGGA 1 cut(s) 526
Ecl136II GAGCTC 1 cut(s) 340
EclXI CGGCCG 4 cut(s) 180, 645, 649, 948
Eco130I CCWWGG 1 cut(s) 942
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 816
Eco52I CGGCCG 4 cut(s) 180, 645, 649, 948
Eco53kI GAGCTC 1 cut(s) 340
Eco88I CYCGRG 2 cut(s) 335, 848
Eco91I GGTNACC 2 cut(s) 895, 1021
EcoICRI GAGCTC 1 cut(s) 340
EcoO65I GGTNACC 2 cut(s) 895, 1021
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 942
EcoT38I GRGCYC 1 cut(s) 342
EgeI GGCGCC 3 cut(s) 478, 553, 738
EheI GGCGCC 3 cut(s) 478, 553, 738
ErhI CCWWGG 1 cut(s) 942
Esp3I CGTCTC 2 cut(s) 367, 460
FaeI CATG 4 cut(s) 21, 295, 736, 1033
FaiI YATR 6 cut(s) 19, 35, 293, 515, 734, 1031
FatI CATG 4 cut(s) 17, 291, 732, 1029
FauI CCCGC 1 cut(s) 374
FblI GTMKAC 3 cut(s) 81, 309, 641
FokI GGATG 3 cut(s) 71, 311, 1018
FriOI GRGCYC 1 cut(s) 342
FseI GGCCGGCC 1 cut(s) 651
FspBI CTAG 1 cut(s) 123
GlaI GCGC 6 cut(s) 304, 400, 478, 553, 738, 913
GsaI CCCAGC 1 cut(s) 92
HaeII RGCGCY 4 cut(s) 402, 480, 555, 740
HaeIII GGCC 6 cut(s) 182, 470, 647, 651, 950, 1055
HapII CCGG 7 cut(s) 46, 102, 648, 652, 982, 989, 1011
HgaI GACGC 5 cut(s) 17, 34, 62, 622, 928
HhaI GCGC 6 cut(s) 305, 401, 479, 554, 739, 914
Hin1I GRCGYC 5 cut(s) 54, 108, 477, 552, 737
Hin1II CATG 4 cut(s) 21, 295, 736, 1033
Hin6I GCGC 6 cut(s) 303, 399, 477, 552, 737, 912
HinP1I GCGC 6 cut(s) 303, 399, 477, 552, 737, 912
HincII GTYRAC 4 cut(s) 82, 642, 877, 918
HindII GTYRAC 4 cut(s) 82, 642, 877, 918
HinfI GANTC 5 cut(s) 169, 219, 533, 576, 695
HpaII CCGG 7 cut(s) 46, 102, 648, 652, 982, 989, 1011
HphI GGTGA 5 cut(s) 511, 907, 1003, 1009, 1033
Hpy166II GTNNAC 7 cut(s) 82, 310, 547, 642, 768, 877, 918
Hpy188I TCNGA 4 cut(s) 152, 218, 575, 700
Hpy188III TCNNGA 3 cut(s) 382, 850, 982
Hpy8I GTNNAC 7 cut(s) 82, 310, 547, 642, 768, 877, 918
Hpy99I CGWCG 9 cut(s) 28, 33, 83, 110, 323, 422, 638, 646, 956
HpyAV CCTTC 4 cut(s) 157, 469, 523, 805
HpyCH4III ACNGT 2 cut(s) 862, 997
HpyCH4IV ACGT 4 cut(s) 108, 318, 349, 789
HpyCH4V TGCA 4 cut(s) 513, 673, 753, 925
HpyF3I CTNAG 2 cut(s) 245, 395
HpySE526I ACGT 4 cut(s) 108, 318, 349, 789
Hsp92I GRCGYC 5 cut(s) 54, 108, 477, 552, 737
Hsp92II CATG 4 cut(s) 21, 295, 736, 1033
HspAI GCGC 6 cut(s) 303, 399, 477, 552, 737, 912
KasI GGCGCC 3 cut(s) 476, 551, 736
Kpn2I TCCGGA 1 cut(s) 981
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
Kzo9I GATC 4 cut(s) 152, 294, 384, 1064
LmnI GCTCC 2 cut(s) 291, 711
Lsp1109I GCAGC 3 cut(s) 711, 747, 909
MaeI CTAG 1 cut(s) 123
MaeII ACGT 4 cut(s) 108, 318, 349, 789
MaeIII GTNAC 4 cut(s) 895, 991, 1021, 1072
MalI GATC 4 cut(s) 154, 296, 386, 1066
MboI GATC 4 cut(s) 152, 294, 384, 1064
MboII GAAGA 5 cut(s) 265, 370, 397, 811, 1072
MflI RGATCY 2 cut(s) 384, 1064
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 1 cut(s) 886
MluI ACGCGT 1 cut(s) 26
Mly113I GGCGCC 3 cut(s) 477, 552, 737
MlyI GAGTC 3 cut(s) 163, 228, 689
MmeI TCCRAC 1 cut(s) 234
MroI TCCGGA 1 cut(s) 981
MroNI GCCGGC 1 cut(s) 647
MslI CAYNNNNRTG 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 604
MspI CCGG 7 cut(s) 46, 102, 648, 652, 982, 989, 1011
MspR9I CCNGG 6 cut(s) 46, 102, 199, 653, 989, 1012
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 4 cut(s) 28, 914, 956, 1019
NaeI GCCGGC 1 cut(s) 649
NarI GGCGCC 3 cut(s) 477, 552, 737
NciI CCSGG 5 cut(s) 46, 102, 653, 989, 1012
NdeII GATC 4 cut(s) 152, 294, 384, 1064
NgoMIV GCCGGC 1 cut(s) 647
NlaIII CATG 4 cut(s) 21, 295, 736, 1033
NlaIV GGNNCC 4 cut(s) 478, 553, 738, 986
NmeAIII GCCGAG 1 cut(s) 565
NmuCI GTSAC 3 cut(s) 895, 991, 1021
PaeR7I CTCGAG 1 cut(s) 335
PaqCI CACCTGC 1 cut(s) 916
PcsI WCGNNNNNNNCGW 1 cut(s) 786
PdiI GCCGGC 1 cut(s) 649
PfeI GAWTC 2 cut(s) 533, 576
PleI GAGTC 3 cut(s) 163, 227, 689
PluTI GGCGCC 3 cut(s) 480, 555, 740
PpsI GAGTC 3 cut(s) 163, 227, 689
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 197
PspEI GGTNACC 2 cut(s) 895, 1021
PspFI CCCAGC 1 cut(s) 88
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 4 cut(s) 478, 553, 738, 986
PspXI VCTCGAGB 1 cut(s) 335
PsrI GAACNNNNNNTAC 2 cut(s) 821, 853
PstI CTGCAG 1 cut(s) 927
PsuI RGATCY 2 cut(s) 384, 1064
RigI GGCCGGCC 1 cut(s) 651
RsaI GTAC 3 cut(s) 203, 830, 869
RsaNI GTAC 3 cut(s) 202, 829, 868
RseI CAYNNNNRTG 1 cut(s) 1034
SacI GAGCTC 1 cut(s) 342
SalI GTCGAC 2 cut(s) 80, 640
Sau3AI GATC 4 cut(s) 152, 294, 384, 1064
SchI GAGTC 3 cut(s) 163, 228, 689
ScrFI CCNGG 6 cut(s) 46, 102, 199, 653, 989, 1012
SduI GDGCHC 1 cut(s) 342
SfcI CTRYAG 2 cut(s) 310, 923
SfoI GGCGCC 3 cut(s) 478, 553, 738
Sfr274I CTCGAG 1 cut(s) 335
SlaI CTCGAG 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 1034
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 1 cut(s) 886
SspDI GGCGCC 3 cut(s) 476, 551, 736
SspMI CTAG 1 cut(s) 123
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 6 cut(s) 44, 100, 197, 651, 987, 1010
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 2 cut(s) 862, 997
TaiI ACGT 4 cut(s) 111, 321, 352, 792
TaqI TCGA 5 cut(s) 81, 336, 641, 713, 780
TasI AATT 1 cut(s) 886
TauI GCSGC 8 cut(s) 185, 372, 429, 604, 749, 797, 950, 959
TfiI GAWTC 2 cut(s) 533, 576
TseFI GTSAC 3 cut(s) 895, 991, 1021
TseI GCWGC 3 cut(s) 724, 760, 922
Tsp45I GTSAC 3 cut(s) 895, 991, 1021
TspDTI ATGAA 1 cut(s) 731
TspGWI ACGGA 2 cut(s) 406, 655
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XhoI CTCGAG 1 cut(s) 335
XmiI GTMKAC 3 cut(s) 81, 309, 641
XspI CTAG 1 cut(s) 123
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.