RLG00000004673

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0020325
Physical Location & Seq
Reverse (-)
61323578 .. 61325295
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004673

Sequence Viewer

Length: 1443 bp
ATGGAGTTGGACAGGATAAGCATCTTAGAAAATGATATTACACAGAAAATTTTCTCATATTTGTCCATCAAGGAGGCAGCGAGGACCAGTGTTCTATCGAAAAACTGGAGGCACAAATGGACTGTGCCTCCATGTCTTGTGTTTGATGATGAAAATGGCCTCTCCACTGAGACAATCGTATCTCATGTTCTTTTACTTCATAATGGCCCAATAGATAGTTTCAAGCTTTCTAGTCGAAAAGATCTCGACCCTAATGATATGGATCAATGGATTCATTATTTATCAATAAACTCTATCAAAGAGATTATAGTGAAAAAAAGGAATAGGTATTCATCATCCTACAGTATCTCTTCCAATTTGTTTTCTTGTCAAGATTTGACTCATTTAGAGTTGTACAATTGTTTGCTGAGACCCCCATCGACATCAAAAGGCTTTGGGATGTTGAAGAGCCTTCATATTCGAAGAGTTACCGTGACCCGAGGCGTGTTAGAAGAAGTGATTTCTCACAGTCCTCTGCTTGAGAAAATAATTTTGTGCAAACTTGGTATCAGACATCTCAAGATTGATGCACCAAATGTCAGTTTTCTTGAACTTCGAGGTGGTTTTGAGGATGTTGAACTTGAGAATACCATAAATCTTGTTGAAGTTTCAATTGACCAAAAACGGGGTTCTACCAATTCTAGCTCATTGCTCAAATTTTTCTTTAAGCTTTCTCGCATCGAGAGACTCACACTCCAGGATAGCTTTTTAGAGTGTGCTAATGGTTCCTTGCCGAAGAAGTTGCCTGAACCATGTCTATGCCTGAACTTTCTTTCTATAAGCATACGCTTGAATAATCTGGAGGATATTTCAACTGCCATGTGCCTTCTGAGGAGCTCACCTGCTCTACAAGAATTAGAAATTTCGGTTCAACAAGACCAACATGTGGCTGATGCCGCAGAAGTAACCGGTTGGTTAAAGGACAAGCAGAATCGACCATTCACTCAACTGCGAATTTTGAAAGTAACTGGCTTCTCATGTGTCAAAGCCGAAATGGACTTCATCGTATTTCTGCTTTTAAGCTCCCCTGCTCTTCAACAACTAGAAATTTCGTTTGGGCGGAAAGCTGTTGAAGTTGTGGACTTTTCTTGGATATATGACAATCAGAATAATGGTGCATTCATGCAACTGCGACTTGTGAAAGTAACCGGATTATCTGGTAATAAAGCTCAAGCGAATTTCATCAGATTTCTTCTTTCAAGTACTTCAACTACGCTTGAGAGGTTGGCTCTTCAACCTGCAGCTTCTGCGAAAGAACTTTGGGAATTATTTAAATTGTTAGGCGAGTTCATCAACCGCGTCTCAGTACCTGCAGACTTGGAGTTCTTGGACCCATCAAATAGTGTGCAGGAGTCTGATGACTCGTCGTCTTCTTCCGATTCTGAAGATGATATTGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

481

Amino Acids

54.17

Weight (kDa)

5.72

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 7 - 43 4.2e-06 F-box domain
LRR_At1g61320_AtMIF1 PF23622 75 - 188 6.6e-09 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 87 - 301 2.2e-14 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 184 - 363 2.6e-09 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020325)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g09540
rosa_chinensis RchiOBHm_Chr7g0188641
rosa_laevigata RLG00000004673
rosa_rugosa Rorug06G0492100
rosa_samantha Rh7DG098900
rosa_wichuraiana Rw7G008370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 889
Acc36I ACCTGC 3 cut(s) 889, 1285, 1357
AccB7I CCANNNNNTGG 1 cut(s) 925
AccII CGCG 1 cut(s) 1338
AciI CCGC 3 cut(s) 936, 1099, 1336
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 6 cut(s) 48, 695, 900, 993, 1086, 1216
AcuI CTGAAG 1 cut(s) 1443
AfaI GTAC 3 cut(s) 395, 1243, 1347
AfiI CCNNNNNNNGG 2 cut(s) 664, 925
AflIII ACRYGT 1 cut(s) 922
AgeI ACCGGT 1 cut(s) 947
AhdI GACNNNNNGTC 1 cut(s) 1405
AjnI CCWGG 1 cut(s) 735
AjuI GAANNNNNNNTTGG 3 cut(s) 1077, 1109, 1416
AluBI AGCT 9 cut(s) 226, 684, 709, 744, 876, 1062, 1106, 1208, 1283
AluI AGCT 9 cut(s) 226, 684, 709, 744, 876, 1062, 1106, 1208, 1283
Alw21I GWGCWC 1 cut(s) 878
Alw26I GTCTC 4 cut(s) 164, 403, 718, 1345
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 2 cut(s) 1286, 1349
Ama87I CYCGRG 1 cut(s) 477
AoxI GGCC 2 cut(s) 157, 205
ApeKI GCWGC 2 cut(s) 77, 1280
ApoI RAATTY 6 cut(s) 48, 695, 900, 993, 1086, 1216
AsiGI ACCGGT 1 cut(s) 947
Asp700I GAANNNNTTC 1 cut(s) 50
AspS9I GGNCC 3 cut(s) 84, 206, 1369
AsuHPI GGTGA 1 cut(s) 870
AsuII TTCGAA 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 2 cut(s) 84, 1369
BaeI ACNNNNGTAYC 2 cut(s) 162, 195
BanII GRGCYC 1 cut(s) 878
BbsI GAAGAC 1 cut(s) 1401
Bbv12I GWGCWC 1 cut(s) 878
BbvI GCAGC 2 cut(s) 89, 1292
BccI CCATC 3 cut(s) 74, 424, 1381
BcgI CGANNNNNNTGC 2 cut(s) 763, 797
BciT130I CCWGG 1 cut(s) 737
BcoDI GTCTC 4 cut(s) 164, 403, 718, 1345
BfaI CTAG 3 cut(s) 231, 681, 1082
BfmI CTRYAG 3 cut(s) 340, 1278, 1350
BfuAI ACCTGC 3 cut(s) 889, 1285, 1357
BglII AGATCT 1 cut(s) 241
BisI GCNGC 3 cut(s) 78, 936, 1281
BlsI GCNGC 3 cut(s) 79, 937, 1282
BmcAI AGTACT 1 cut(s) 1243
Bme1390I CCNGG 1 cut(s) 737
Bme18I GGWCC 2 cut(s) 84, 1369
BmeRI GACNNNNNGTC 1 cut(s) 1405
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 3 cut(s) 84, 206, 1369
BmiI GGNNCC 2 cut(s) 766, 1371
BmrFI CCNGG 1 cut(s) 737
BmsI GCATC 4 cut(s) 30, 556, 726, 922
BpiI GAAGAC 1 cut(s) 1401
BplI GAGNNNNNCTC 2 cut(s) 1252, 1284
BpmI CTGGAG 3 cut(s) 127, 719, 860
Bpu14I TTCGAA 1 cut(s) 460
BpuEI CTTGAG 5 cut(s) 539, 542, 641, 1194, 1277
BsaBI GATNNNNATC 2 cut(s) 20, 261
BsaI GGTCTC 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 478
BsaWI WCCGGW 2 cut(s) 947, 1187
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 717, 747
Bsc4I CCNNNNNNNGG 2 cut(s) 664, 925
Bse118I RCCGGY 1 cut(s) 947
Bse1I ACTGG 3 cut(s) 87, 110, 1012
Bse3DI GCAATG 1 cut(s) 686
Bse8I GATNNNNATC 2 cut(s) 20, 261
BseBI CCWGG 1 cut(s) 737
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 3 cut(s) 335, 444, 616
BseJI GATNNNNATC 2 cut(s) 20, 261
BseLI CCNNNNNNNGG 2 cut(s) 664, 925
BseMI GCAATG 1 cut(s) 686
BseMII CTCAG 4 cut(s) 159, 398, 860, 1356
BseNI ACTGG 3 cut(s) 87, 110, 1012
BseRI GAGGAG 1 cut(s) 886
BseXI GCAGC 2 cut(s) 89, 1292
BsgI GTGCAG 1 cut(s) 1406
Bsh1236I CGCG 1 cut(s) 1338
BshFI GGCC 2 cut(s) 159, 207
BshTI ACCGGT 1 cut(s) 947
BsiHKAI GWGCWC 1 cut(s) 878
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 2 cut(s) 948, 1188
BslI CCNNNNNNNGG 2 cut(s) 664, 925
BsmAI GTCTC 4 cut(s) 164, 403, 718, 1345
BsmBI CGTCTC 1 cut(s) 1345
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 2 cut(s) 159, 207
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 477
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 1 cut(s) 878
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 2 cut(s) 241, 262
BspACI CCGC 3 cut(s) 936, 1099, 1336
BspANI GGCC 2 cut(s) 159, 207
BspCNI CTCAG 4 cut(s) 160, 399, 861, 1355
BspFNI CGCG 1 cut(s) 1338
BspLI GGNNCC 2 cut(s) 766, 1371
BspMAI CTGCAG 2 cut(s) 1282, 1354
BspMI ACCTGC 3 cut(s) 889, 1285, 1357
BspPI GGATC 1 cut(s) 270
BspQI GCTCTTC 3 cut(s) 440, 1077, 1275
BspT104I TTCGAA 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 947
BsrGI TGTACA 1 cut(s) 393
BsrI ACTGG 3 cut(s) 87, 110, 1012
BssAI RCCGGY 1 cut(s) 947
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 2 cut(s) 241, 262
Bst2UI CCWGG 1 cut(s) 737
Bst4CI ACNGT 4 cut(s) 124, 344, 472, 509
Bst6I CTCTTC 5 cut(s) 355, 440, 457, 1077, 1275
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstAUI TGTACA 1 cut(s) 393
BstBI TTCGAA 1 cut(s) 460
BstDEI CTNAG 5 cut(s) 25, 168, 407, 869, 1342
BstF5I GGATG 3 cut(s) 335, 444, 616
BstFNI CGCG 1 cut(s) 1338
BstKTI GATC 2 cut(s) 244, 265
BstMAI GTCTC 4 cut(s) 164, 403, 718, 1345
BstMBI GATC 2 cut(s) 241, 262
BstMWI GCNNNNNNNGC 2 cut(s) 935, 1286
BstNI CCWGG 1 cut(s) 737
BstNSI RCATGY 1 cut(s) 926
BstSCI CCNGG 1 cut(s) 735
BstSFI CTRYAG 3 cut(s) 340, 1278, 1350
BstUI CGCG 1 cut(s) 1338
BstV1I GCAGC 2 cut(s) 89, 1292
BstV2I GAAGAC 1 cut(s) 1401
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 2 cut(s) 159, 207
BtsCI GGATG 3 cut(s) 335, 444, 616
BtsIMutI CAGTG 2 cut(s) 94, 165
BveI ACCTGC 3 cut(s) 889, 1285, 1357
CaiI CAGNNNCTG 2 cut(s) 1286, 1349
Cfr10I RCCGGY 1 cut(s) 947
Cfr13I GGNCC 3 cut(s) 84, 206, 1369
CseI GACGC 1 cut(s) 1327
Csp6I GTAC 3 cut(s) 394, 1242, 1346
CspAI ACCGGT 1 cut(s) 947
CviAII CATG 7 cut(s) 132, 185, 792, 859, 923, 1017, 1162
CviQI GTAC 3 cut(s) 394, 1242, 1346
DdeI CTNAG 5 cut(s) 25, 168, 407, 869, 1342
DpnI GATC 2 cut(s) 243, 264
DpnII GATC 2 cut(s) 241, 262
DraI TTTAAA 1 cut(s) 1312
DriI GACNNNNNGTC 1 cut(s) 1405
Eam1104I CTCTTC 5 cut(s) 355, 440, 457, 1077, 1275
Eam1105I GACNNNNNGTC 1 cut(s) 1405
EarI CTCTTC 5 cut(s) 355, 440, 457, 1077, 1275
EciI GGCGGA 1 cut(s) 1114
Ecl136II GAGCTC 1 cut(s) 876
Eco24I GRGCYC 1 cut(s) 878
Eco31I GGTCTC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 84, 1369
Eco53kI GAGCTC 1 cut(s) 876
Eco57I CTGAAG 1 cut(s) 1443
Eco88I CYCGRG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 876
EcoRII CCWGG 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 878
Esp3I CGTCTC 1 cut(s) 1345
FaeI CATG 7 cut(s) 135, 188, 795, 862, 926, 1020, 1165
FatI CATG 7 cut(s) 131, 184, 791, 858, 922, 1016, 1161
Fnu4HI GCNGC 3 cut(s) 78, 936, 1281
FokI GGATG 3 cut(s) 322, 451, 623
FriOI GRGCYC 1 cut(s) 878
Fsp4HI GCNGC 3 cut(s) 78, 936, 1281
FspBI CTAG 3 cut(s) 231, 681, 1082
GluI GCNGC 3 cut(s) 78, 936, 1281
GsuI CTGGAG 3 cut(s) 127, 719, 860
HaeIII GGCC 2 cut(s) 159, 207
HapII CCGG 2 cut(s) 948, 1188
HgaI GACGC 1 cut(s) 1327
Hin1II CATG 7 cut(s) 135, 188, 795, 862, 926, 1020, 1165
HindIII AAGCTT 2 cut(s) 224, 707
HinfI GANTC 7 cut(s) 271, 379, 726, 970, 1391, 1400, 1418
HpaII CCGG 2 cut(s) 948, 1188
HphI GGTGA 1 cut(s) 870
Hpy166II GTNNAC 1 cut(s) 1120
Hpy188I TCNGA 7 cut(s) 551, 870, 1146, 1226, 1396, 1417, 1423
Hpy188III TCNNGA 6 cut(s) 245, 371, 559, 587, 721, 839
Hpy8I GTNNAC 1 cut(s) 1120
Hpy99I CGWCG 1 cut(s) 1408
HpyAV CCTTC 2 cut(s) 461, 875
HpyCH4III ACNGT 4 cut(s) 124, 344, 472, 509
HpyCH4V TGCA 7 cut(s) 537, 569, 1157, 1165, 1280, 1352, 1387
HpyF10VI GCNNNNNNNGC 2 cut(s) 935, 1286
HpyF3I CTNAG 5 cut(s) 25, 168, 407, 869, 1342
Hsp92II CATG 7 cut(s) 135, 188, 795, 862, 926, 1020, 1165
Kzo9I GATC 2 cut(s) 241, 262
LguI GCTCTTC 3 cut(s) 440, 1077, 1275
LmnI GCTCC 2 cut(s) 873, 1067
Lsp1109I GCAGC 2 cut(s) 89, 1292
LweI GCATC 4 cut(s) 30, 556, 726, 922
MaeI CTAG 3 cut(s) 231, 681, 1082
MaeIII GTNAC 5 cut(s) 466, 472, 943, 1003, 1183
MalI GATC 2 cut(s) 243, 264
MboI GATC 2 cut(s) 241, 262
MfeI CAATTG 2 cut(s) 397, 651
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 878
MlyI GAGTC 4 cut(s) 373, 720, 1394, 1400
MroXI GAANNNNTTC 1 cut(s) 50
MseI TTAA 4 cut(s) 705, 956, 1058, 1311
MslI CAYNNNNRTG 1 cut(s) 796
MspI CCGG 2 cut(s) 948, 1188
MspR9I CCNGG 1 cut(s) 737
MunI CAATTG 2 cut(s) 397, 651
Mva1269I GAATGC 1 cut(s) 1157
MvaI CCWGG 1 cut(s) 737
MvnI CGCG 1 cut(s) 1338
MwoI GCNNNNNNNGC 2 cut(s) 935, 1286
NdeII GATC 2 cut(s) 241, 262
NlaIII CATG 7 cut(s) 135, 188, 795, 862, 926, 1020, 1165
NlaIV GGNNCC 2 cut(s) 766, 1371
NmuCI GTSAC 1 cut(s) 472
NspI RCATGY 1 cut(s) 926
NspV TTCGAA 1 cut(s) 460
PaqCI CACCTGC 1 cut(s) 889
PciI ACATGT 1 cut(s) 922
PciSI GCTCTTC 3 cut(s) 440, 1077, 1275
PctI GAATGC 1 cut(s) 1157
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 3 cut(s) 271, 970, 1418
PflMI CCANNNNNTGG 1 cut(s) 925
PfoI TCCNGGA 1 cut(s) 735
PinAI ACCGGT 1 cut(s) 947
PkrI GCNGC 3 cut(s) 79, 937, 1282
PleI GAGTC 4 cut(s) 373, 720, 1394, 1399
PpsI GAGTC 4 cut(s) 373, 720, 1394, 1399
PscI ACATGT 1 cut(s) 922
Psp124BI GAGCTC 1 cut(s) 878
Psp6I CCWGG 1 cut(s) 735
PspGI CCWGG 1 cut(s) 735
PspN4I GGNNCC 2 cut(s) 766, 1371
PspPI GGNCC 3 cut(s) 84, 206, 1369
PsrI GAACNNNNNNTAC 2 cut(s) 171, 203
PstI CTGCAG 2 cut(s) 1282, 1354
PstNI CAGNNNCTG 2 cut(s) 1286, 1349
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 3 cut(s) 395, 1243, 1347
RsaNI GTAC 3 cut(s) 394, 1242, 1346
RseI CAYNNNNRTG 1 cut(s) 796
SacI GAGCTC 1 cut(s) 878
SapI GCTCTTC 3 cut(s) 440, 1077, 1275
SaqAI TTAA 4 cut(s) 705, 956, 1058, 1311
SatI GCNGC 3 cut(s) 78, 936, 1281
Sau3AI GATC 2 cut(s) 241, 262
Sau96I GGNCC 3 cut(s) 84, 206, 1369
ScaI AGTACT 1 cut(s) 1243
SchI GAGTC 4 cut(s) 373, 720, 1394, 1400
ScrFI CCNGG 1 cut(s) 737
SduI GDGCHC 1 cut(s) 878
SfaNI GCATC 4 cut(s) 30, 556, 726, 922
SfcI CTRYAG 3 cut(s) 340, 1278, 1350
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 2 cut(s) 84, 1369
SmiI ATTTAAAT 1 cut(s) 1312
SmiMI CAYNNNNRTG 1 cut(s) 796
SmlI CTYRAG 5 cut(s) 518, 557, 620, 1209, 1256
SmoI CTYRAG 5 cut(s) 518, 557, 620, 1209, 1256
SsiI CCGC 3 cut(s) 936, 1099, 1336
SspMI CTAG 3 cut(s) 231, 681, 1082
SstI GAGCTC 1 cut(s) 878
StyD4I CCNGG 1 cut(s) 735
SwaI ATTTAAAT 1 cut(s) 1312
TaaI ACNGT 4 cut(s) 124, 344, 472, 509
TaqI TCGA 8 cut(s) 98, 235, 246, 419, 460, 595, 720, 973
TatI WGTACW 2 cut(s) 393, 1241
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 3 cut(s) 271, 970, 1418
Tru1I TTAA 4 cut(s) 705, 956, 1058, 1311
Tru9I TTAA 4 cut(s) 705, 956, 1058, 1311
TscAI CASTG 2 cut(s) 94, 172
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 2 cut(s) 77, 1280
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 9 cut(s) 165, 188, 263, 321, 443, 1030, 1150, 1210, 1318
TspRI CASTG 2 cut(s) 94, 172
Van91I CCANNNNNTGG 1 cut(s) 925
VpaK11BI GGWCC 2 cut(s) 84, 1369
XapI RAATTY 6 cut(s) 48, 695, 900, 993, 1086, 1216
XceI RCATGY 1 cut(s) 926
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 3 cut(s) 231, 681, 1082
ZrmI AGTACT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.