RLG00000008388

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0022804
Physical Location & Seq
Reverse (-)
29937090 .. 29937581
492 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008388

Sequence Viewer

Length: 408 bp
ATGAGTTTGGCTTTTGATACAGTTGGTTTATTGTGTGTGCATTTACTCGCTTGCTATTTTTCGACTTCCACCGGACCTGTGGCCGGGACTCTGTATCTGTCGACGGCAAGAATCAGTTTTTGCAGCGACCGGCCGTTGGCTTTTACTGCTCCGTCTGGACAATCAACTTGGAGCTACTACAAGGTGATGATACCTATGACGAATGTAGGGACAGTGAATCCTGTGGTGATTACGGAGACCCTGACCCCGGAGAAGTACCTTCAGATTGTCACCACCGATGGACATGATTTCTGGTTTATGGGGTTTGTTAACTTCGAGGAAGCTTCGCATCACGTACTAGACAGTGTGTCGAATTTCAGAGCAGTTGGGACCAATGAAGTGCAACAAGTGCAACCTGTTGTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

136

Amino Acids

14.79

Weight (kDa)

5.07

Isoelectric Point (pI)

25.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 17 - 110 3.5e-10 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022804)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0310061
rosa_laevigata RLG00000008388
rosa_samantha Rh6AG490800 Rh6BG500300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 101
AcoI YGGCCR 2 cut(s) 81, 131
AcsI RAATTY 1 cut(s) 352
AcuI CTGAAG 1 cut(s) 245
AfaI GTAC 2 cut(s) 257, 336
AfiI CCNNNNNNNGG 3 cut(s) 83, 136, 247
AhdI GACNNNNNGTC 1 cut(s) 346
AluBI AGCT 2 cut(s) 174, 323
AluI AGCT 2 cut(s) 174, 323
Alw26I GTCTC 1 cut(s) 230
AoxI GGCC 2 cut(s) 81, 131
ApeKI GCWGC 1 cut(s) 123
ApoI RAATTY 1 cut(s) 352
AspS9I GGNCC 2 cut(s) 74, 369
AsuC2I CCSGG 2 cut(s) 85, 248
AsuHPI GGTGA 3 cut(s) 196, 238, 262
AvaII GGWCC 2 cut(s) 74, 369
BbvI GCAGC 1 cut(s) 135
BccI CCATC 1 cut(s) 272
BceAI ACGGC 2 cut(s) 118, 120
BcnI CCSGG 2 cut(s) 85, 248
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 1 cut(s) 338
BisI GCNGC 1 cut(s) 124
BlsI GCNGC 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 85, 248
Bme18I GGWCC 2 cut(s) 74, 369
BmeRI GACNNNNNGTC 1 cut(s) 346
BmgT120I GGNCC 2 cut(s) 74, 369
BmiI GGNNCC 1 cut(s) 370
BmrFI CCNGG 2 cut(s) 85, 248
BmsI GCATC 1 cut(s) 337
BpuMI CCSGG 2 cut(s) 85, 248
BsaAI YACGTR 1 cut(s) 334
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 246
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 136, 247
Bse118I RCCGGY 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 246
BseLI CCNNNNNNNGG 3 cut(s) 83, 136, 247
BseX3I CGGCCG 1 cut(s) 131
BseXI GCAGC 1 cut(s) 135
Bsh1285I CGRYCG 2 cut(s) 130, 134
BshFI GGCC 2 cut(s) 83, 133
BsiEI CGRYCG 2 cut(s) 130, 134
BsiSI CCGG 4 cut(s) 72, 84, 130, 248
BslFI GGGAC 3 cut(s) 100, 223, 382
BslI CCNNNNNNNGG 3 cut(s) 83, 136, 247
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 3 cut(s) 100, 223, 382
BsnI GGCC 2 cut(s) 83, 133
Bso31I GGTCTC 1 cut(s) 230
BspANI GGCC 2 cut(s) 83, 133
BspLI GGNNCC 1 cut(s) 370
BspTNI GGTCTC 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 246
Bst4CI ACNGT 3 cut(s) 22, 214, 344
BstAPI GCANNNNNTGC 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 1 cut(s) 405
BstMAI GTCTC 1 cut(s) 230
BstMCI CGRYCG 2 cut(s) 130, 134
BstMWI GCNNNNNNNGC 2 cut(s) 146, 388
BstSCI CCNGG 2 cut(s) 83, 246
BstV1I GCAGC 1 cut(s) 135
BstZI CGGCCG 1 cut(s) 131
BsuRI GGCC 2 cut(s) 83, 133
BtsIMutI CAGTG 2 cut(s) 219, 349
Cac8I GCNNGC 1 cut(s) 52
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 2 cut(s) 74, 369
Csp6I GTAC 2 cut(s) 256, 335
CviAII CATG 1 cut(s) 284
CviJI RGCY 6 cut(s) 11, 83, 133, 140, 174, 323
CviKI_1 RGCY 6 cut(s) 11, 83, 133, 140, 174, 323
CviQI GTAC 2 cut(s) 256, 335
DdeI CTNAG 1 cut(s) 405
DriI GACNNNNNGTC 1 cut(s) 346
EaeI YGGCCR 2 cut(s) 81, 131
EagI CGGCCG 1 cut(s) 131
Eam1105I GACNNNNNGTC 1 cut(s) 346
EclXI CGGCCG 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 74, 369
Eco52I CGGCCG 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 245
FaeI CATG 1 cut(s) 287
FaiI YATR 3 cut(s) 197, 285, 299
FaqI GGGAC 3 cut(s) 100, 223, 382
FatI CATG 1 cut(s) 283
FblI GTMKAC 1 cut(s) 101
Fnu4HI GCNGC 1 cut(s) 124
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 1 cut(s) 338
GluI GCNGC 1 cut(s) 124
HaeIII GGCC 2 cut(s) 83, 133
HapII CCGG 4 cut(s) 72, 84, 130, 248
Hin1II CATG 1 cut(s) 287
HincII GTYRAC 2 cut(s) 102, 310
HindII GTYRAC 2 cut(s) 102, 310
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 3 cut(s) 88, 111, 217
HpaI GTTAAC 1 cut(s) 310
HpaII CCGG 4 cut(s) 72, 84, 130, 248
HphI GGTGA 3 cut(s) 196, 238, 262
Hpy166II GTNNAC 2 cut(s) 102, 310
Hpy188I TCNGA 2 cut(s) 264, 359
Hpy188III TCNNGA 1 cut(s) 156
Hpy8I GTNNAC 2 cut(s) 102, 310
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 3 cut(s) 22, 214, 344
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 4 cut(s) 40, 123, 382, 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 388
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 1 cut(s) 287
KspAI GTTAAC 1 cut(s) 310
LmnI GCTCC 2 cut(s) 154, 171
LpnPI CCDG 9 cut(s) 85, 90, 97, 141, 143, 234, 254, 261, 277
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 268
MluCI AATT 1 cut(s) 352
MlyI GAGTC 1 cut(s) 82
MnlI CCTC 1 cut(s) 310
MseI TTAA 1 cut(s) 309
MspI CCGG 4 cut(s) 72, 84, 130, 248
MspR9I CCNGG 2 cut(s) 85, 248
MwoI GCNNNNNNNGC 2 cut(s) 146, 388
NciI CCSGG 2 cut(s) 85, 248
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 268
PfeI GAWTC 2 cut(s) 111, 217
PkrI GCNGC 1 cut(s) 125
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Ppu21I YACGTR 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 2 cut(s) 74, 369
RsaI GTAC 2 cut(s) 257, 336
RsaNI GTAC 2 cut(s) 256, 335
SalI GTCGAC 1 cut(s) 100
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 124
Sau96I GGNCC 2 cut(s) 74, 369
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 85, 248
SetI ASST 8 cut(s) 79, 176, 186, 196, 261, 325, 336, 397
SfaNI GCATC 1 cut(s) 337
SinI GGWCC 2 cut(s) 74, 369
Sse9I AATT 1 cut(s) 352
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 2 cut(s) 83, 246
TaaI ACNGT 3 cut(s) 22, 214, 344
TaiI ACGT 1 cut(s) 336
TaqI TCGA 4 cut(s) 62, 101, 315, 350
TasI AATT 1 cut(s) 352
TfiI GAWTC 2 cut(s) 111, 217
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 219, 349
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 123
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 390
TspGWI ACGGA 2 cut(s) 141, 248
TspRI CASTG 2 cut(s) 219, 349
VpaK11BI GGWCC 2 cut(s) 74, 369
XapI RAATTY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 101
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.