Rh6AG490800

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0022804
Physical Location & Seq
Forward (+)
66737843 .. 66741530
3688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG490800.1

Sequence Viewer

Length: 531 bp
ATGGCTCAAGATCACCACGCCATATATGTATCTGAGTCTCGAGCCATGTGGGTTGGTGGTGAAGCTAAGCCAAAACCAATCGTGGATCAAACCCCAACAACGAACAAAAGCCTATCCTCCCGCAATTGCAGTGGGAGTTTGGCTTTTGATACAGTTGGTTTATTGTGTGTGCATTTATTCGCTTGCTATCTTTCGACTTCCACCGGACCTGTGGCTGGGACTCTGTATCTGTCGACGGCAAGAATCGGTTTTTGTAGCGACCGACCGTTGACTTTTACTGCTCCGTCTGGACAATCAACTTGGAGCTACTACAAGGTGATGATACCTATGATGAATGTAGGGACAATGAATCCTGTGGTGATTACAGAGATCCCGACGCTGGAGAAGTACCTTCAGATTGTCACCACCGATGGACATGATTTCTGGTTTATGGGGTTTGTTAACTTTGAGAAAGCTTCGCATCATGTACTAGACAGTGTGTCGAATTTCAGAGCAGTTGGGACCAATGAAGTGCAACCTGTTCTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

176

Amino Acids

19.26

Weight (kDa)

6.27

Isoelectric Point (pI)

23.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 60 - 152 2.5e-08 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022804)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0310061
rosa_laevigata RLG00000008388
rosa_samantha Rh6AG490800 Rh6BG500300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 233
AciI CCGC 1 cut(s) 121
AclWI GGATC 2 cut(s) 93, 364
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 2 cut(s) 389, 468
AfiI CCNNNNNNNGG 2 cut(s) 215, 379
AhdI GACNNNNNGTC 1 cut(s) 478
AluBI AGCT 3 cut(s) 65, 306, 455
AluI AGCT 3 cut(s) 65, 306, 455
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 2 cut(s) 93, 364
Ama87I CYCGRG 1 cut(s) 39
ApoI RAATTY 1 cut(s) 484
AspS9I GGNCC 2 cut(s) 206, 501
AsuHPI GGTGA 5 cut(s) 5, 71, 328, 370, 394
AvaI CYCGRG 1 cut(s) 39
AvaII GGWCC 2 cut(s) 206, 501
BccI CCATC 1 cut(s) 404
BceAI ACGGC 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 42
BfaI CTAG 1 cut(s) 470
BlpI GCTNAGC 1 cut(s) 66
Bme18I GGWCC 2 cut(s) 206, 501
BmeRI GACNNNNNGTC 1 cut(s) 478
BmeT110I CYCGRG 1 cut(s) 39
BmgT120I GGNCC 2 cut(s) 206, 501
BmiI GGNNCC 1 cut(s) 502
BmsI GCATC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 401
Bpu1102I GCTNAGC 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 203
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 379
BseLI CCNNNNNNNGG 2 cut(s) 215, 379
BseMII CTCAG 1 cut(s) 24
BseYI CCCAGC 1 cut(s) 215
Bsh1285I CGRYCG 2 cut(s) 262, 266
BsiEI CGRYCG 2 cut(s) 262, 266
BsiHKCI CYCGRG 1 cut(s) 39
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 3 cut(s) 232, 355, 514
BslI CCNNNNNNNGG 2 cut(s) 215, 379
BsmAI GTCTC 1 cut(s) 42
BsmFI GGGAC 3 cut(s) 232, 355, 514
BsoBI CYCGRG 1 cut(s) 39
Bsp143I GATC 3 cut(s) 10, 85, 369
Bsp1720I GCTNAGC 1 cut(s) 66
BspACI CCGC 1 cut(s) 121
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 502
BspPI GGATC 2 cut(s) 93, 364
BssMI GATC 3 cut(s) 10, 85, 369
Bst4CI ACNGT 3 cut(s) 154, 267, 476
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 3 cut(s) 33, 66, 528
BstKTI GATC 3 cut(s) 13, 88, 372
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 3 cut(s) 10, 85, 369
BstMCI CGRYCG 2 cut(s) 262, 266
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 481
Cac8I GCNNGC 1 cut(s) 184
Cfr13I GGNCC 2 cut(s) 206, 501
CseI GACGC 1 cut(s) 385
Csp6I GTAC 2 cut(s) 388, 467
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 3 cut(s) 46, 416, 464
CviJI RGCY 9 cut(s) 5, 44, 65, 70, 111, 143, 215, 306, 455
CviKI_1 RGCY 9 cut(s) 5, 44, 65, 70, 111, 143, 215, 306, 455
CviQI GTAC 2 cut(s) 388, 467
DdeI CTNAG 3 cut(s) 33, 66, 528
DpnI GATC 3 cut(s) 12, 87, 371
DpnII GATC 3 cut(s) 10, 85, 369
DriI GACNNNNNGTC 1 cut(s) 478
Eam1105I GACNNNNNGTC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 206, 501
Eco57I CTGAAG 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 39
FaeI CATG 3 cut(s) 49, 419, 467
FaiI YATR 8 cut(s) 23, 25, 27, 47, 329, 417, 431, 465
FaqI GGGAC 3 cut(s) 232, 355, 514
FatI CATG 3 cut(s) 45, 415, 463
FauI CCCGC 1 cut(s) 128
FblI GTMKAC 1 cut(s) 233
FspBI CTAG 1 cut(s) 470
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 401
HapII CCGG 1 cut(s) 204
HgaI GACGC 1 cut(s) 385
Hin1II CATG 3 cut(s) 49, 419, 467
HincII GTYRAC 3 cut(s) 234, 270, 442
HindII GTYRAC 3 cut(s) 234, 270, 442
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 4 cut(s) 35, 220, 243, 349
HpaI GTTAAC 1 cut(s) 442
HpaII CCGG 1 cut(s) 204
HphI GGTGA 5 cut(s) 5, 71, 328, 370, 394
Hpy166II GTNNAC 3 cut(s) 234, 270, 442
Hpy188I TCNGA 3 cut(s) 34, 396, 491
Hpy188III TCNNGA 4 cut(s) 8, 39, 288, 373
Hpy8I GTNNAC 3 cut(s) 234, 270, 442
Hpy99I CGWCG 2 cut(s) 238, 379
HpyAV CCTTC 1 cut(s) 401
HpyCH4III ACNGT 3 cut(s) 154, 267, 476
HpyCH4V TGCA 3 cut(s) 129, 172, 514
HpyF3I CTNAG 3 cut(s) 33, 66, 528
Hsp92II CATG 3 cut(s) 49, 419, 467
KspAI GTTAAC 1 cut(s) 442
Kzo9I GATC 3 cut(s) 10, 85, 369
LmnI GCTCC 2 cut(s) 286, 303
LpnPI CCDG 7 cut(s) 201, 217, 222, 273, 365, 366, 409
LweI GCATC 1 cut(s) 469
MaeI CTAG 1 cut(s) 470
MaeIII GTNAC 1 cut(s) 400
MalI GATC 3 cut(s) 12, 87, 371
MboI GATC 3 cut(s) 10, 85, 369
MfeI CAATTG 1 cut(s) 124
MflI RGATCY 1 cut(s) 369
MluCI AATT 2 cut(s) 124, 484
MlyI GAGTC 2 cut(s) 44, 214
MnlI CCTC 1 cut(s) 127
MseI TTAA 1 cut(s) 441
MspI CCGG 1 cut(s) 204
MunI CAATTG 1 cut(s) 124
NdeII GATC 3 cut(s) 10, 85, 369
NlaIII CATG 3 cut(s) 49, 419, 467
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 39
PfeI GAWTC 2 cut(s) 243, 349
PleI GAGTC 2 cut(s) 43, 214
PpsI GAGTC 2 cut(s) 43, 214
PspFI CCCAGC 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 2 cut(s) 206, 501
PsuI RGATCY 1 cut(s) 369
RsaI GTAC 2 cut(s) 389, 468
RsaNI GTAC 2 cut(s) 388, 467
SalI GTCGAC 1 cut(s) 232
SaqAI TTAA 1 cut(s) 441
Sau3AI GATC 3 cut(s) 10, 85, 369
Sau96I GGNCC 2 cut(s) 206, 501
SchI GAGTC 2 cut(s) 44, 214
SetI ASST 8 cut(s) 67, 211, 308, 318, 328, 393, 457, 520
SfaNI GCATC 1 cut(s) 469
Sfr274I CTCGAG 1 cut(s) 39
SinI GGWCC 2 cut(s) 206, 501
SlaI CTCGAG 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 6, 39
SmoI CTYRAG 2 cut(s) 6, 39
Sse9I AATT 2 cut(s) 124, 484
SsiI CCGC 1 cut(s) 121
SspMI CTAG 1 cut(s) 470
TaaI ACNGT 3 cut(s) 154, 267, 476
TaqI TCGA 4 cut(s) 40, 194, 233, 482
TaqII GACCGA 1 cut(s) 276
TasI AATT 2 cut(s) 124, 484
TatI WGTACW 1 cut(s) 466
TfiI GAWTC 2 cut(s) 243, 349
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 2 cut(s) 136, 481
TseFI GTSAC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 3 cut(s) 347, 362, 522
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 2 cut(s) 136, 481
VpaK11BI GGWCC 2 cut(s) 206, 501
XapI RAATTY 1 cut(s) 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XhoI CTCGAG 1 cut(s) 39
XmiI GTMKAC 1 cut(s) 233
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.