RLG00000010190

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0021517
Physical Location & Seq
Forward (+)
63993656 .. 63994991
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010190

Sequence Viewer

Length: 1134 bp
ATGGGATTATTACCACTCCTTTTAGGTGCGTTACTACTACTCCTAGAGACTTCAGGAACGCTGCCCCAAGCACTGCCTCATTGCCAGGACCAATGTGGTAACGTGTCCATACCATATCCCTTTGGTATAACCAAGGGATGTTATTGGTCCCCAGATTTCCTGATTCATTGTATTATTAGTGGTACTGGATCCCCAGTACCGTATTTTTGGGGCCGTCAAATCACTAATATAGCTCCTGAGACAAGTGAGTTACACGTCTTGGAGGGTGTAACTTCGACGTGCCATAATGAGACTCAGATAGCCATTGATGAGAAGACCGAGCTTCAGTTGCCTCTACATTTCACGATATCTCAGGAAAAGAATAGCCTCTTCGGCGTCGGGTGTGGTACCACAGCAATTTTTAGGGAGGAACATGATAACTACTTAAAACATGGCTCATGGGAAATCGAGTATACTAGTTTTTGCACTTCTAGATGTGATGACAGTCTAGATGACTTCCAGCTACGGAGGGGCACCATGTGTTCTGGATTTGGTTGTTGTCGCCTATCCATTTCGAAGGCAGTGCAGAACTTGACCATCGTGGTGGGAAATAATCAGAATGAGACCGAGTGGTACTACAAAAGATACCCATGCAGTTATGCATTCGTTGCAGAAAATGACCACTTCTTATTCTCTCCCACTACGAGCTTAGACGATCTTAGCAGTAGAACCCATCTTCCTATGGTCCTTAATTGGGATATTGGTGGTGGTACATGTAGCACGAGGAGTCCAGCATGCAGCTTGTTTAGCAAGTGCATAGACGCTAGTGGAAGTTCAGGTGCTTATATTTGCCAGTGTTTGTCGGGTTACAACGGAAACCCATACCTCCCAGATGGGTGTCATGACATTGATGAGTGCAAGCGAAACCCTTGTACAATGGGAACTTGCACCAATACACCAGGTAGTTACTTCTGCTCTGTGAGCGAACAGAGGCGATGGATTATTATTGTTTCAGGTGTGGTTGCAATTGCTCTACTCATTTTCGTCATCCTTTGGTTGAGAGGATTTAGATGTTGTTTGAGATCGTCTGGAGAACAAGGCAAGTTTTATTTTGGCAATGTTACTTCTTTTTGTTGGTCTTACCTAAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

378

Amino Acids

41.83

Weight (kDa)

5.37

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 85 8.4e-08 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021517)

Species Orthologous Gene IDs
rosa_laevigata RLG00000010190
rosa_multiflora Rmu_sc0002157.1_g000029 Rmu_sc0007400.1_g000004 Rmu_sc0038730.1_g000001
rosa_samantha Rh4BG004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 386
AccB1I GGYRCC 2 cut(s) 386, 512
AccI GTMKAC 1 cut(s) 452
AclWI GGATC 2 cut(s) 183, 196
AcuI CTGAAG 2 cut(s) 36, 308
AcyI GRCGYC 1 cut(s) 375
AdeI CACNNNGTG 1 cut(s) 519
AfaI GTAC 6 cut(s) 184, 198, 388, 614, 751, 913
AfiI CCNNNNNNNGG 1 cut(s) 733
AflIII ACRYGT 3 cut(s) 102, 253, 752
AhlI ACTAGT 1 cut(s) 455
AjiI CACGTC 2 cut(s) 256, 279
AjnI CCWGG 2 cut(s) 84, 937
AloI GAACNNNNNNTCC 2 cut(s) 700, 732
AluBI AGCT 5 cut(s) 233, 322, 502, 687, 780
AluI AGCT 5 cut(s) 233, 322, 502, 687, 780
Alw26I GTCTC 4 cut(s) 41, 233, 284, 596
AlwI GGATC 2 cut(s) 183, 196
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 61, 777
Asp718I GGTACC 1 cut(s) 386
AspS9I GGNCC 4 cut(s) 88, 147, 211, 724
AsuII TTCGAA 1 cut(s) 554
AvaII GGWCC 3 cut(s) 88, 147, 724
BaeGI GKGCMC 1 cut(s) 515
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 2 cut(s) 386, 512
BauI CACGAG 1 cut(s) 760
BbsI GAAGAC 1 cut(s) 320
BbvI GCAGC 2 cut(s) 48, 789
BccI CCATC 4 cut(s) 584, 720, 866, 969
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 2 cut(s) 86, 939
BcoDI GTCTC 4 cut(s) 41, 233, 284, 596
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 5 cut(s) 44, 456, 471, 488, 804
BglI GCCNNNNNGGC 1 cut(s) 372
BisI GCNGC 2 cut(s) 62, 778
BlsI GCNGC 2 cut(s) 63, 779
Bme1390I CCNGG 2 cut(s) 86, 939
Bme18I GGWCC 3 cut(s) 88, 147, 724
BmgBI CACGTC 2 cut(s) 256, 279
BmgT120I GGNCC 4 cut(s) 88, 147, 211, 724
BmiI GGNNCC 5 cut(s) 149, 190, 212, 388, 514
BmrFI CCNGG 2 cut(s) 86, 939
BmrI ACTGGG 1 cut(s) 188
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 1 cut(s) 1089
Bpu14I TTCGAA 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 1 cut(s) 733
Bse1I ACTGG 3 cut(s) 190, 194, 832
Bse3DI GCAATG 2 cut(s) 79, 1102
BseBI CCWGG 2 cut(s) 86, 939
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 143, 1026
BseLI CCNNNNNNNGG 1 cut(s) 733
BseMI GCAATG 2 cut(s) 79, 1102
BseMII CTCAG 3 cut(s) 228, 308, 365
BseNI ACTGG 3 cut(s) 190, 194, 832
BseRI GAGGAG 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 515
BseXI GCAGC 2 cut(s) 48, 789
BsgI GTGCAG 1 cut(s) 584
BshFI GGCC 1 cut(s) 213
BshNI GGYRCC 2 cut(s) 386, 512
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 733
BsmAI GTCTC 4 cut(s) 41, 233, 284, 596
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 515
Bsp1407I TGTACA 1 cut(s) 911
Bsp143I GATC 3 cut(s) 188, 694, 1061
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 3 cut(s) 229, 307, 364
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 5 cut(s) 149, 190, 212, 388, 514
BspPI GGATC 2 cut(s) 183, 196
BspT104I TTCGAA 1 cut(s) 554
BspT107I GGYRCC 2 cut(s) 386, 512
BspTNI GGTCTC 1 cut(s) 596
BsrDI GCAATG 2 cut(s) 79, 1102
BsrGI TGTACA 1 cut(s) 911
BsrI ACTGG 3 cut(s) 190, 194, 832
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 3 cut(s) 188, 694, 1061
BssNAI GTATAC 1 cut(s) 453
BssNI GRCGYC 1 cut(s) 375
BssSI CACGAG 1 cut(s) 760
BssT1I CCWWGG 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 760
Bst2UI CCWGG 2 cut(s) 86, 939
Bst4CI ACNGT 2 cut(s) 201, 485
Bst6I CTCTTC 1 cut(s) 374
BstACI GRCGYC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 647
BstAUI TGTACA 1 cut(s) 911
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 775, 899
BstDEI CTNAG 5 cut(s) 237, 294, 351, 688, 698
BstF5I GGATG 2 cut(s) 143, 1026
BstKTI GATC 3 cut(s) 191, 697, 1064
BstMAI GTCTC 4 cut(s) 41, 233, 284, 596
BstMBI GATC 3 cut(s) 188, 694, 1061
BstMWI GCNNNNNNNGC 5 cut(s) 328, 372, 647, 786, 960
BstNI CCWGG 2 cut(s) 86, 939
BstNSI RCATGY 2 cut(s) 756, 777
BstSCI CCNGG 2 cut(s) 84, 937
BstSLI GKGCMC 1 cut(s) 515
BstV1I GCAGC 2 cut(s) 48, 789
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 188
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 188
BstZ17I GTATAC 1 cut(s) 453
BsuRI GGCC 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 988
BtrI CACGTC 2 cut(s) 256, 279
BtsCI GGATG 2 cut(s) 143, 1026
BtsI GCAGTG 2 cut(s) 71, 567
BtsIMutI CAGTG 3 cut(s) 71, 567, 839
Cac8I GCNNGC 2 cut(s) 775, 899
CciI TCATGA 1 cut(s) 880
Cfr13I GGNCC 4 cut(s) 88, 147, 211, 724
CseI GACGC 2 cut(s) 364, 809
CsiI ACCWGGT 1 cut(s) 937
Csp6I GTAC 6 cut(s) 183, 197, 387, 613, 750, 912
CviAII CATG 8 cut(s) 413, 431, 438, 517, 630, 753, 774, 881
CviJI RGCY 9 cut(s) 213, 233, 302, 322, 366, 435, 502, 687, 780
CviKI_1 RGCY 9 cut(s) 213, 233, 302, 322, 366, 435, 502, 687, 780
CviQI GTAC 6 cut(s) 183, 197, 387, 613, 750, 912
DdeI CTNAG 5 cut(s) 237, 294, 351, 688, 698
DpnI GATC 3 cut(s) 190, 696, 1063
DpnII GATC 3 cut(s) 188, 694, 1061
DraIII CACNNNGTG 1 cut(s) 519
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 596
Eco32I GATATC 1 cut(s) 348
Eco47I GGWCC 3 cut(s) 88, 147, 724
Eco57I CTGAAG 2 cut(s) 36, 308
EcoRII CCWGG 2 cut(s) 84, 937
EcoRV GATATC 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 8 cut(s) 416, 434, 441, 520, 633, 756, 777, 884
FaqI GGGAC 1 cut(s) 133
FatI CATG 8 cut(s) 412, 430, 437, 516, 629, 752, 773, 880
FblI GTMKAC 1 cut(s) 452
Fnu4HI GCNGC 2 cut(s) 62, 778
FokI GGATG 2 cut(s) 150, 1013
Fsp4HI GCNGC 2 cut(s) 62, 778
FspBI CTAG 5 cut(s) 44, 456, 471, 488, 804
GluI GCNGC 2 cut(s) 62, 778
GsuI CTGGAG 1 cut(s) 1089
HaeIII GGCC 1 cut(s) 213
HgaI GACGC 2 cut(s) 364, 809
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 8 cut(s) 416, 434, 441, 520, 633, 756, 777, 884
HinfI GANTC 3 cut(s) 163, 292, 766
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 2 cut(s) 297, 597
Hpy8I GTNNAC 1 cut(s) 453
Hpy99I CGWCG 2 cut(s) 280, 380
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 2 cut(s) 201, 485
HpyCH4IV ACGT 3 cut(s) 102, 255, 278
HpyF10VI GCNNNNNNNGC 5 cut(s) 328, 372, 647, 786, 960
HpyF3I CTNAG 5 cut(s) 237, 294, 351, 688, 698
HpySE526I ACGT 3 cut(s) 102, 255, 278
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 8 cut(s) 416, 434, 441, 520, 633, 756, 777, 884
KpnI GGTACC 1 cut(s) 390
Kzo9I GATC 3 cut(s) 188, 694, 1061
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 2 cut(s) 48, 789
MabI ACCWGGT 1 cut(s) 937
MaeI CTAG 5 cut(s) 44, 456, 471, 488, 804
MaeII ACGT 3 cut(s) 102, 255, 278
MaeIII GTNAC 7 cut(s) 30, 98, 249, 268, 845, 944, 1099
MalI GATC 3 cut(s) 190, 696, 1063
MboI GATC 3 cut(s) 188, 694, 1061
MboII GAAGA 3 cut(s) 325, 361, 707
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 3 cut(s) 396, 730, 1005
MlyI GAGTC 2 cut(s) 286, 775
Mph1103I ATGCAT 1 cut(s) 643
MseI TTAA 2 cut(s) 425, 729
MslI CAYNNNNRTG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 86, 939
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 2 cut(s) 86, 939
MwoI GCNNNNNNNGC 5 cut(s) 328, 372, 647, 786, 960
NdeII GATC 3 cut(s) 188, 694, 1061
NlaIII CATG 8 cut(s) 416, 434, 441, 520, 633, 756, 777, 884
NlaIV GGNNCC 5 cut(s) 149, 190, 212, 388, 514
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 2 cut(s) 756, 777
NspV TTCGAA 1 cut(s) 554
PaeI GCATGC 1 cut(s) 777
PagI TCATGA 1 cut(s) 880
PciI ACATGT 1 cut(s) 752
PctI GAATGC 1 cut(s) 641
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 2 cut(s) 63, 779
PleI GAGTC 2 cut(s) 286, 774
PpsI GAGTC 2 cut(s) 286, 774
PscI ACATGT 1 cut(s) 752
Psp6I CCWGG 2 cut(s) 84, 937
PspGI CCWGG 2 cut(s) 84, 937
PspN4I GGNNCC 5 cut(s) 149, 190, 212, 388, 514
PspPI GGNCC 4 cut(s) 88, 147, 211, 724
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 6 cut(s) 184, 198, 388, 614, 751, 913
RsaNI GTAC 6 cut(s) 183, 197, 387, 613, 750, 912
RseI CAYNNNNRTG 1 cut(s) 581
SaqAI TTAA 2 cut(s) 425, 729
SatI GCNGC 2 cut(s) 62, 778
Sau3AI GATC 3 cut(s) 188, 694, 1061
Sau96I GGNCC 4 cut(s) 88, 147, 211, 724
SchI GAGTC 2 cut(s) 286, 775
ScrFI CCNGG 2 cut(s) 86, 939
SduI GDGCHC 1 cut(s) 515
SexAI ACCWGGT 1 cut(s) 937
SfuI TTCGAA 1 cut(s) 554
SinI GGWCC 3 cut(s) 88, 147, 724
SmiMI CAYNNNNRTG 1 cut(s) 581
SpeI ACTAGT 1 cut(s) 455
SphI GCATGC 1 cut(s) 777
Sse9I AATT 3 cut(s) 396, 730, 1005
SspMI CTAG 5 cut(s) 44, 456, 471, 488, 804
StyD4I CCNGG 2 cut(s) 84, 937
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 2 cut(s) 201, 485
TaiI ACGT 3 cut(s) 105, 258, 281
TaqI TCGA 3 cut(s) 275, 447, 554
TaqII GACCGA 2 cut(s) 332, 620
TasI AATT 3 cut(s) 396, 730, 1005
TatI WGTACW 1 cut(s) 911
TfiI GAWTC 1 cut(s) 163
Tru1I TTAA 2 cut(s) 425, 729
Tru9I TTAA 2 cut(s) 425, 729
TscAI CASTG 3 cut(s) 78, 567, 839
TseI GCWGC 2 cut(s) 61, 777
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 2 cut(s) 520, 867
TspRI CASTG 3 cut(s) 78, 567, 839
VpaK11BI GGWCC 3 cut(s) 88, 147, 724
XbaI TCTAGA 2 cut(s) 470, 487
XceI RCATGY 2 cut(s) 756, 777
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 452
XspI CTAG 5 cut(s) 44, 456, 471, 488, 804
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.