Rh4BG004900

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0021517
Physical Location & Seq
Forward (+)
887054 .. 900991
13938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG004900.1

Sequence Viewer

Length: 741 bp
ATGTCCTGCCCGGTTGCCACGATCGATGTGGTACATGTGCAGTTGCTTCCCTATTATGCCTCATTTTCATTATTGTATGGTTCAGAAGGTTTAGAGGCCGCAAATCTGGAGAACAAGGTCCTAGTTAGGATGACTAACATACCCCCACACGTTGTTGCGAAGATAAGGAATTTTTCTAAATTCACCATCCCCATTTCCCCACCCCTACATAGGACACGAGGCAAACGAGAAACATTGTTCATTAGCCCTATTCATAGCATTCCCTGTCGGACCTTCATTGGGTACATGGCGCCAGAGTTTGTTTTGCACGGTCAATTCAATGACAAGATTGATAATTATAGTTTTGGAGTACTTGCATTGGAGCTGATATCTGGAAAGAGGCTTACAGATTCGCCAACAGATGAAGGCCTTGTATGCTGGGCTCGTGATTTAAACAAGAAAGAAAACCTACTGCAATTGGTAGATCCAAGTTTCGGTGAAAACTGCAGGAAAGACGAAGTGATTAGAGCAATCAAAATTGCTCTACTTTGTACTAGTCAATCACACGAACAAAGACCTGCAATGACCGAAGTTGTGAAGATTCTTCAAGGTGAAGTTACCATTCCCGAGTTGGATATGCATTCTGATAGTGATGCTGAGGCGCCACCTGAGGTTCAGTCTATATTTGCTGGGATATTGGAGGAGGGTCGAATGAGAAATGAACACAGAAAACTTGAGACCGAAAGCACCCCACTGCAGTAG

Protein Analysis

246

Amino Acids

27.64

Weight (kDa)

5.78

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 91 - 195 5e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021517)

Species Orthologous Gene IDs
rosa_laevigata RLG00000010190
rosa_multiflora Rmu_sc0002157.1_g000029 Rmu_sc0007400.1_g000004 Rmu_sc0038730.1_g000001
rosa_samantha Rh4BG004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 2 cut(s) 289, 640
AciI CCGC 1 cut(s) 99
AclWI GGATC 1 cut(s) 458
AcsI RAATTY 2 cut(s) 169, 179
AcyI GRCGYC 2 cut(s) 290, 641
AfaI GTAC 4 cut(s) 33, 284, 351, 532
AfiI CCNNNNNNNGG 3 cut(s) 210, 279, 473
AflIII ACRYGT 2 cut(s) 34, 148
AgsI TTSAA 2 cut(s) 319, 587
AhlI ACTAGT 1 cut(s) 533
AluBI AGCT 1 cut(s) 364
AluI AGCT 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 710
AlwI GGATC 1 cut(s) 458
Ama87I CYCGRG 1 cut(s) 605
AoxI GGCC 2 cut(s) 96, 406
ApoI RAATTY 2 cut(s) 169, 179
AspLEI GCGC 2 cut(s) 292, 643
AspS9I GGNCC 2 cut(s) 118, 270
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 175, 488, 602
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 2 cut(s) 118, 270
AxyI CCTNAGG 1 cut(s) 648
BanI GGYRCC 2 cut(s) 289, 640
BanII GRGCYC 1 cut(s) 424
BauI CACGAG 2 cut(s) 216, 423
BbvCI CCTCAGC 1 cut(s) 636
BccI CCATC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 1 cut(s) 710
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 2 cut(s) 122, 534
BfmI CTRYAG 2 cut(s) 484, 734
BfoI RGCGCY 2 cut(s) 293, 644
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
BmcAI AGTACT 1 cut(s) 351
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 2 cut(s) 118, 270
BmeT110I CYCGRG 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 118, 270
BmiI GGNNCC 2 cut(s) 291, 642
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 1 cut(s) 622
BpmI CTGGAG 1 cut(s) 128
Bpu10I CCTNAGC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 24
BsaHI GRCGYC 2 cut(s) 290, 641
BsaI GGTCTC 1 cut(s) 710
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 279, 473
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 1 cut(s) 567
BseCI ATCGAT 1 cut(s) 24
BseGI GGATG 2 cut(s) 135, 186
BseLI CCNNNNNNNGG 3 cut(s) 210, 279, 473
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 2 cut(s) 627, 639
BseRI GAGGAG 1 cut(s) 695
BseYI CCCAGC 2 cut(s) 417, 668
BsgI GTGCAG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 98, 408
BshNI GGYRCC 2 cut(s) 289, 640
BshVI ATCGAT 1 cut(s) 24
BsiEI CGRYCG 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 1 cut(s) 11
BslI CCNNNNNNNGG 3 cut(s) 210, 279, 473
BsmAI GTCTC 1 cut(s) 710
BsmI GAATGC 2 cut(s) 258, 619
BsnI GGCC 2 cut(s) 98, 408
Bso31I GGTCTC 1 cut(s) 710
BsoBI CYCGRG 1 cut(s) 605
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 2 cut(s) 21, 463
BspACI CCGC 1 cut(s) 99
BspANI GGCC 2 cut(s) 98, 408
BspCNI CTCAG 2 cut(s) 628, 640
BspDI ATCGAT 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 291, 642
BspMAI CTGCAG 2 cut(s) 488, 738
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 1 cut(s) 458
BspT107I GGYRCC 2 cut(s) 289, 640
BspTNI GGTCTC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 567
BssMI GATC 2 cut(s) 21, 463
BssNI GRCGYC 2 cut(s) 290, 641
BssSI CACGAG 2 cut(s) 216, 423
Bst2BI CACGAG 2 cut(s) 216, 423
Bst4CI ACNGT 1 cut(s) 311
BstACI GRCGYC 2 cut(s) 290, 641
BstDEI CTNAG 2 cut(s) 636, 648
BstF5I GGATG 2 cut(s) 135, 186
BstH2I RGCGCY 2 cut(s) 293, 644
BstHHI GCGC 2 cut(s) 292, 643
BstKTI GATC 2 cut(s) 24, 466
BstMAI GTCTC 1 cut(s) 710
BstMBI GATC 2 cut(s) 21, 463
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 2 cut(s) 484, 734
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
Bsu15I ATCGAT 1 cut(s) 24
Bsu36I CCTNAGG 1 cut(s) 648
BsuRI GGCC 2 cut(s) 98, 408
BsuTUI ATCGAT 1 cut(s) 24
BtsCI GGATG 2 cut(s) 135, 186
BtsI GCAGTG 1 cut(s) 731
BtsIMutI CAGTG 1 cut(s) 731
BveI ACCTGC 1 cut(s) 565
CfoI GCGC 2 cut(s) 292, 643
Cfr13I GGNCC 2 cut(s) 118, 270
ClaI ATCGAT 1 cut(s) 24
Csp6I GTAC 4 cut(s) 32, 283, 350, 531
CviAII CATG 2 cut(s) 35, 286
CviJI RGCY 6 cut(s) 98, 246, 364, 382, 408, 422
CviKI_1 RGCY 6 cut(s) 98, 246, 364, 382, 408, 422
CviQI GTAC 4 cut(s) 32, 283, 350, 531
DdeI CTNAG 2 cut(s) 636, 648
DinI GGCGCC 2 cut(s) 291, 642
DpnI GATC 2 cut(s) 23, 465
DpnII GATC 2 cut(s) 21, 463
DraI TTTAAA 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 408
Eco24I GRGCYC 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 710
Eco32I GATATC 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 118, 270
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 605
EcoO109I RGGNCCY 1 cut(s) 118
EcoRV GATATC 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 621
EcoT38I GRGCYC 1 cut(s) 424
EgeI GGCGCC 2 cut(s) 291, 642
EheI GGCGCC 2 cut(s) 291, 642
FaeI CATG 2 cut(s) 38, 289
FatI CATG 2 cut(s) 34, 285
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 2 cut(s) 142, 173
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 2 cut(s) 122, 534
GlaI GCGC 2 cut(s) 291, 642
GluI GCNGC 1 cut(s) 99
GsaI CCCAGC 2 cut(s) 421, 672
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 2 cut(s) 293, 644
HaeIII GGCC 2 cut(s) 98, 408
HapII CCGG 1 cut(s) 11
HhaI GCGC 2 cut(s) 292, 643
Hin1I GRCGYC 2 cut(s) 290, 641
Hin1II CATG 2 cut(s) 38, 289
Hin6I GCGC 2 cut(s) 290, 641
HinP1I GCGC 2 cut(s) 290, 641
HinfI GANTC 2 cut(s) 389, 580
HpaII CCGG 1 cut(s) 11
HphI GGTGA 3 cut(s) 175, 488, 602
Hpy188I TCNGA 3 cut(s) 85, 270, 625
Hpy188III TCNNGA 4 cut(s) 107, 372, 425, 605
HpyAV CCTTC 3 cut(s) 80, 283, 398
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 8 cut(s) 40, 307, 356, 454, 486, 560, 619, 736
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpyF3I CTNAG 2 cut(s) 636, 648
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 2 cut(s) 290, 641
Hsp92II CATG 2 cut(s) 38, 289
HspAI GCGC 2 cut(s) 290, 641
KasI GGCGCC 2 cut(s) 289, 640
Kzo9I GATC 2 cut(s) 21, 463
LmnI GCTCC 1 cut(s) 361
LweI GCATC 1 cut(s) 622
MaeI CTAG 2 cut(s) 122, 534
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 595
MalI GATC 2 cut(s) 23, 465
MboI GATC 2 cut(s) 21, 463
MboII GAAGA 3 cut(s) 172, 575, 589
MfeI CAATTG 1 cut(s) 455
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 1 cut(s) 424
MluCI AATT 6 cut(s) 169, 179, 314, 334, 455, 516
Mly113I GGCGCC 2 cut(s) 290, 641
MmeI TCCRAC 2 cut(s) 248, 591
MnlI CCTC 8 cut(s) 70, 88, 212, 372, 631, 643, 673, 676
Mph1103I ATGCAT 1 cut(s) 621
MseI TTAA 1 cut(s) 431
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 11
MunI CAATTG 1 cut(s) 455
Mva1269I GAATGC 2 cut(s) 258, 619
MwoI GCNNNNNNNGC 1 cut(s) 414
NarI GGCGCC 2 cut(s) 290, 641
NciI CCSGG 1 cut(s) 11
NdeII GATC 2 cut(s) 21, 463
NlaIII CATG 2 cut(s) 38, 289
NlaIV GGNNCC 2 cut(s) 291, 642
NsiI ATGCAT 1 cut(s) 621
NspI RCATGY 1 cut(s) 38
PceI AGGCCT 1 cut(s) 408
PciI ACATGT 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 223
PctI GAATGC 2 cut(s) 258, 619
PfeI GAWTC 2 cut(s) 389, 580
PkrI GCNGC 1 cut(s) 100
Ple19I CGATCG 1 cut(s) 24
PluTI GGCGCC 2 cut(s) 293, 644
PpuMI RGGWCCY 1 cut(s) 118
PscI ACATGT 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 118
PspFI CCCAGC 2 cut(s) 417, 668
PspN4I GGNNCC 2 cut(s) 291, 642
PspPI GGNCC 2 cut(s) 118, 270
PspPPI RGGWCCY 1 cut(s) 118
PstI CTGCAG 2 cut(s) 488, 738
PsuI RGATCY 1 cut(s) 463
PvuI CGATCG 1 cut(s) 24
RsaI GTAC 4 cut(s) 33, 284, 351, 532
RsaNI GTAC 4 cut(s) 32, 283, 350, 531
SaqAI TTAA 1 cut(s) 431
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 2 cut(s) 21, 463
Sau96I GGNCC 2 cut(s) 118, 270
ScaI AGTACT 1 cut(s) 351
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 1 cut(s) 622
SfcI CTRYAG 2 cut(s) 484, 734
SfoI GGCGCC 2 cut(s) 291, 642
SinI GGWCC 2 cut(s) 118, 270
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 6 cut(s) 169, 179, 314, 334, 455, 516
SseBI AGGCCT 1 cut(s) 408
SsiI CCGC 1 cut(s) 99
SspDI GGCGCC 2 cut(s) 289, 640
SspMI CTAG 2 cut(s) 122, 534
StuI AGGCCT 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 1 cut(s) 153
TaqI TCGA 2 cut(s) 24, 688
TaqII GACCGA 2 cut(s) 581, 734
TasI AATT 6 cut(s) 169, 179, 314, 334, 455, 516
TatI WGTACW 2 cut(s) 349, 530
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 389, 580
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 1 cut(s) 738
TspDTI ATGAA 6 cut(s) 57, 229, 242, 265, 417, 714
TspRI CASTG 1 cut(s) 738
VpaK11BI GGWCC 2 cut(s) 118, 270
XapI RAATTY 2 cut(s) 169, 179
XceI RCATGY 1 cut(s) 38
XcmI CCANNNNNNNNNTGG 2 cut(s) 25, 607
XspI CTAG 2 cut(s) 122, 534
ZrmI AGTACT 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.