RLG00000012495

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016261
Physical Location & Seq
Forward (+)
19289998 .. 19292937
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012495

Sequence Viewer

Length: 1455 bp
ATGGCTCCACAAAGACGATTATTCTCGTTCTGTATTTTTAATGTCCTCTCCTTTAGTACTATAACTTCAGGCTATGGTGGTACTTTGCCAGATAACAAACCAAGCACTCAGCGCTTCGCTCCTAAGTACTCATACAAATTGGGTAAATCGGTTCCCCGGGAGGCCGGTCCTGAAAGGCTCTCCCTTCACACATTGGGTAGCACACTGCAGTCAAAGCGGATGGTGACTAATCCTTTTCATCCCTTTCACAATGTGAGTGGCGCACGACCATCAACTCCGGCTCCGATTAGCGTTGATAAGTTTGGAGCTAAAGGGATTGATCATACAGATGACACAGAGGCATTTCAGAAGGCGTGGCTGGAAGCTTGTTCCTCCAACGGAGCGGTGGTTCTTCTGGTGCCGCCCCACAGGAGCTATTTCGTTAAGCAATTCACATTCTCAGGCGCGCAATGCAAATCCCATATTACAATGCAGATCCAAGGAACGATAATGGCATTTGAGGATCCGAAAACCTACCATTTTCCAAATAAAAGTAAGCAGCAGTACTGGCTTACCTTTGAGAGGGTTCAGAACTTGGTGGTTGAAGGTCCTGGAACCATCAATGGCAACGGAGAAAAATGGTGGCAAAGCTCATGCAAACAAAACAATATAACTCGCACGCTGCCCAATTGCAATAACGACGCACCAACTGCTGTAACCTTCAAGAAGTGCAATAACTTGACGGTGCATAATCTGAATTTTGAAAAGGCACAGCAGAAGCATGTTTCGTTTGAAAATTGCACGGATGTTAAAGCTTCCCGTCTCAGAATAAATACATCAGAGGCTAGTCCCAACACTGATGGAATTCACGTTACAGAAACCAAAAACATCGACATTTCAATGAGTCGTATAAGCACAGGTGATGATTGTATATCCATTATGGGAGGATCCCAGAATGTTTACGTCGCAAACATAGCTTGCGGACCAGGCCATGGAATCAGCATTGGTAGCTTGGGAAGAGGTGGCTCAGAAGACCATGTTTCCGACGTAACAGTCTTTGGATCTAGGCTAACTGGAACCATGTATGGAGTGAGGATCAAGACATGGCCGGGAGGGTCAGGGTATGCAACCAACATCACATTTCAGATGATACAGATGGATAATGTGACCAACCCCATAATCATCGACCAAAACTACTGCGACACTGCAACCAAAGTCATCAACGGAGAATGCAAACCACCACCCCTGCAGAAAAATACAGCAGTTAAAGTGCATAATGTTATGTACCAGAGCATAAATGGGACAGTTTCTAAGGACGGCCAAGCCATAGTATTTAATTGCAGCAGTGTTCCTGGTGCATGTGAGAAGATTGCGCTGGACGATGTTAGACTTGAGAAAGGAGGAGCTGCTGTATGCAGCAACGTCAAACCGACTTACAGCAGAGTAGATTTGCCCCTCAACCGATGCCGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

485

Amino Acids

52.95

Weight (kDa)

9.04

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 123 - 470 1e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20980
rosa_chinensis RchiOBHm_Chr6g0287361
rosa_laevigata RLG00000012495
rosa_multiflora Rmu_sc0007029.1_g000028
rosa_roxburghii Rroxscaffold_7G00181500
rosa_rugosa Rorug06G0187300 Rorug06G0187400 Rorug06G0187500 Rorug06G0187600.1
rosa_samantha Rh6AG298800 Rh6BG304300 Rh6CG305300 Rh6CG312900 Rh6DG296200
rosa_wichuraiana Rw6G025820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1031
AccB1I GGYRCC 1 cut(s) 397
AccB7I CCANNNNNTGG 1 cut(s) 971
AccBSI CCGCTC 1 cut(s) 383
AccII CGCG 1 cut(s) 446
AciI CCGC 4 cut(s) 217, 383, 401, 960
AclWI GGATC 7 cut(s) 469, 497, 510, 921, 934, 1048, 1082
AcoI YGGCCR 2 cut(s) 1085, 1297
AcsI RAATTY 2 cut(s) 736, 843
AcuI CTGAAG 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 253
AfaI GTAC 5 cut(s) 58, 82, 128, 545, 1265
AfeI AGCGCT 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 561, 971
AgsI TTSAA 5 cut(s) 584, 703, 743, 773, 879
AjnI CCWGG 3 cut(s) 589, 964, 1330
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 8 cut(s) 308, 365, 414, 630, 794, 956, 990, 1385
AluI AGCT 8 cut(s) 308, 365, 414, 630, 794, 956, 990, 1385
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 7 cut(s) 469, 497, 510, 921, 934, 1048, 1082
Ama87I CYCGRG 1 cut(s) 156
Aor51HI AGCGCT 1 cut(s) 113
AoxI GGCC 4 cut(s) 162, 967, 1085, 1297
ApeKI GCWGC 5 cut(s) 538, 661, 1320, 1385, 1395
ApoI RAATTY 2 cut(s) 736, 843
AspLEI GCGC 5 cut(s) 114, 263, 446, 448, 1354
AspS9I GGNCC 3 cut(s) 167, 587, 962
AsuC2I CCSGG 3 cut(s) 157, 158, 1089
AsuHPI GGTGA 2 cut(s) 235, 911
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 3 cut(s) 167, 587, 962
BamHI GGATCC 2 cut(s) 502, 926
BanI GGYRCC 1 cut(s) 397
BarI GAAGNNNNNNTAC 2 cut(s) 49, 81
BbsI GAAGAC 1 cut(s) 1017
BbvI GCAGC 5 cut(s) 550, 648, 1332, 1372, 1407
BccI CCATC 5 cut(s) 214, 277, 605, 833, 1129
BceAI ACGGC 2 cut(s) 1312, 1431
BciT130I CCWGG 3 cut(s) 591, 966, 1332
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 3 cut(s) 157, 158, 1089
BcoDI GTCTC 1 cut(s) 806
BfaI CTAG 2 cut(s) 825, 1044
BfmI CTRYAG 2 cut(s) 206, 1226
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 6 cut(s) 401, 539, 662, 1321, 1386, 1396
BlsI GCNGC 6 cut(s) 402, 540, 663, 1322, 1387, 1397
BmcAI AGTACT 3 cut(s) 58, 128, 545
Bme1390I CCNGG 6 cut(s) 157, 158, 591, 966, 1089, 1332
Bme18I GGWCC 3 cut(s) 167, 587, 962
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 3 cut(s) 167, 587, 962
BmiI GGNNCC 8 cut(s) 6, 153, 282, 399, 504, 595, 928, 1057
BmrFI CCNGG 6 cut(s) 157, 158, 591, 966, 1089, 1332
BmsI GCATC 1 cut(s) 1433
BpiI GAAGAC 1 cut(s) 1017
BpuEI CTTGAG 1 cut(s) 1391
BpuMI CCSGG 3 cut(s) 157, 158, 1089
BsaJI CCNNGG 4 cut(s) 155, 156, 478, 970
BsaXI ACNNNNNCTCC 6 cut(s) 265, 295, 372, 402, 1374, 1404
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 971
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 551, 1057
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 3 cut(s) 591, 966, 1332
BseDI CCNNGG 4 cut(s) 155, 156, 478, 970
BseGI GGATG 3 cut(s) 225, 238, 790
BseLI CCNNNNNNNGG 2 cut(s) 561, 971
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 4 cut(s) 122, 453, 817, 1020
BseNI ACTGG 2 cut(s) 551, 1057
BsePI GCGCGC 1 cut(s) 444
BseRI GAGGAG 1 cut(s) 1395
BseXI GCAGC 5 cut(s) 550, 648, 1332, 1372, 1407
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 4 cut(s) 164, 969, 1087, 1299
BshNI GGYRCC 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 4 cut(s) 157, 165, 278, 1088
BslFI GGGAC 2 cut(s) 813, 1294
BslI CCNNNNNNNGG 2 cut(s) 561, 971
BsmAI GTCTC 1 cut(s) 806
BsmBI CGTCTC 1 cut(s) 806
BsmFI GGGAC 2 cut(s) 813, 1294
BsmI GAATGC 1 cut(s) 1214
BsnI GGCC 4 cut(s) 164, 969, 1087, 1299
BsoBI CYCGRG 1 cut(s) 156
Bsp143I GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
Bsp19I CCATGG 1 cut(s) 970
BspACI CCGC 4 cut(s) 217, 383, 401, 960
BspANI GGCC 4 cut(s) 164, 969, 1087, 1299
BspCNI CTCAG 4 cut(s) 121, 452, 816, 1019
BspFNI CGCG 1 cut(s) 446
BspLI GGNNCC 8 cut(s) 6, 153, 282, 399, 504, 595, 928, 1057
BspMAI CTGCAG 2 cut(s) 210, 1230
BspPI GGATC 7 cut(s) 469, 497, 510, 921, 934, 1048, 1082
BspT107I GGYRCC 1 cut(s) 397
BsrBI CCGCTC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 2 cut(s) 551, 1057
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 155, 156, 478, 970
BssHII GCGCGC 1 cut(s) 444
BssMI GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
BssT1I CCWWGG 2 cut(s) 478, 970
Bst2UI CCWGG 3 cut(s) 591, 966, 1332
Bst4CI ACNGT 3 cut(s) 724, 1033, 1285
Bst6I CTCTTC 1 cut(s) 991
BstAPI GCANNNNNTGC 1 cut(s) 689
BstC8I GCNNGC 3 cut(s) 446, 659, 958
BstDEI CTNAG 6 cut(s) 108, 123, 439, 803, 1006, 1290
BstDSI CCRYGG 1 cut(s) 970
BstENI CCTNNNNNAGG 1 cut(s) 559
BstF5I GGATG 3 cut(s) 225, 238, 790
BstFNI CGCG 1 cut(s) 446
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 5 cut(s) 114, 263, 446, 448, 1354
BstKTI GATC 6 cut(s) 322, 477, 505, 929, 1043, 1077
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
BstMWI GCNNNNNNNGC 8 cut(s) 111, 214, 450, 547, 689, 953, 966, 987
BstNI CCWGG 3 cut(s) 591, 966, 1332
BstNSI RCATGY 2 cut(s) 764, 1341
BstSCI CCNGG 6 cut(s) 155, 156, 589, 964, 1087, 1330
BstSFI CTRYAG 2 cut(s) 206, 1226
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 5 cut(s) 550, 648, 1332, 1372, 1407
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 4 cut(s) 474, 502, 926, 1040
BstYI RGATCY 4 cut(s) 474, 502, 926, 1040
BsuRI GGCC 4 cut(s) 164, 969, 1087, 1299
BtgI CCRYGG 1 cut(s) 970
BtsCI GGATG 3 cut(s) 225, 238, 790
BtsI GCAGTG 3 cut(s) 203, 1182, 1330
BtsIMutI CAGTG 4 cut(s) 203, 834, 1182, 1330
Cac8I GCNNGC 3 cut(s) 446, 659, 958
CfoI GCGC 5 cut(s) 114, 263, 446, 448, 1354
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 3 cut(s) 167, 587, 962
Cfr9I CCCGGG 1 cut(s) 156
CseI GACGC 1 cut(s) 689
Csp6I GTAC 5 cut(s) 57, 81, 127, 544, 1264
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 7 cut(s) 633, 761, 971, 1016, 1060, 1083, 1338
CviQI GTAC 5 cut(s) 57, 81, 127, 544, 1264
DdeI CTNAG 6 cut(s) 108, 123, 439, 803, 1006, 1290
DpnI GATC 6 cut(s) 321, 476, 504, 928, 1042, 1076
DpnII GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
DraIII CACNNNGTG 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 1031
DseDI GACNNNNNNGTC 1 cut(s) 1031
EaeI YGGCCR 2 cut(s) 1085, 1297
Eam1104I CTCTTC 1 cut(s) 991
EarI CTCTTC 1 cut(s) 991
Eco130I CCWWGG 2 cut(s) 478, 970
Eco47I GGWCC 3 cut(s) 167, 587, 962
Eco47III AGCGCT 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 156
EcoNI CCTNNNNNAGG 1 cut(s) 559
EcoO109I RGGNCCY 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 843
EcoRII CCWGG 3 cut(s) 589, 964, 1330
EcoT14I CCWWGG 2 cut(s) 478, 970
ErhI CCWWGG 2 cut(s) 478, 970
Esp3I CGTCTC 1 cut(s) 806
FaeI CATG 7 cut(s) 636, 764, 974, 1019, 1063, 1086, 1341
FaqI GGGAC 2 cut(s) 813, 1294
FatI CATG 7 cut(s) 632, 760, 970, 1015, 1059, 1082, 1337
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 6 cut(s) 401, 539, 662, 1321, 1386, 1396
FokI GGATG 3 cut(s) 225, 232, 797
Fsp4HI GCNGC 6 cut(s) 401, 539, 662, 1321, 1386, 1396
FspBI CTAG 2 cut(s) 825, 1044
GlaI GCGC 5 cut(s) 113, 262, 445, 447, 1353
GluI GCNGC 6 cut(s) 401, 539, 662, 1321, 1386, 1396
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 4 cut(s) 164, 969, 1087, 1299
HapII CCGG 4 cut(s) 157, 165, 278, 1088
HgaI GACGC 1 cut(s) 689
HhaI GCGC 5 cut(s) 114, 263, 446, 448, 1354
Hin1II CATG 7 cut(s) 636, 764, 974, 1019, 1063, 1086, 1341
Hin6I GCGC 5 cut(s) 112, 261, 444, 446, 1352
HinP1I GCGC 5 cut(s) 112, 261, 444, 446, 1352
HindIII AAGCTT 2 cut(s) 363, 792
HinfI GANTC 2 cut(s) 883, 975
HpaII CCGG 4 cut(s) 157, 165, 278, 1088
HphI GGTGA 2 cut(s) 235, 911
Hpy166II GTNNAC 1 cut(s) 940
Hpy188III TCNNGA 3 cut(s) 170, 703, 1078
Hpy8I GTNNAC 1 cut(s) 940
Hpy99I CGWCG 3 cut(s) 683, 947, 1028
HpyAV CCTTC 4 cut(s) 194, 343, 578, 709
HpyCH4III ACNGT 3 cut(s) 724, 1033, 1285
HpyCH4IV ACGT 4 cut(s) 849, 942, 1026, 1401
HpyF10VI GCNNNNNNNGC 8 cut(s) 111, 214, 450, 547, 689, 953, 966, 987
HpyF3I CTNAG 6 cut(s) 108, 123, 439, 803, 1006, 1290
HpySE526I ACGT 4 cut(s) 849, 942, 1026, 1401
Hsp92II CATG 7 cut(s) 636, 764, 974, 1019, 1063, 1086, 1341
HspAI GCGC 5 cut(s) 112, 261, 444, 446, 1352
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
LmnI GCTCC 7 cut(s) 10, 124, 286, 305, 380, 411, 1382
Lsp1109I GCAGC 5 cut(s) 550, 648, 1332, 1372, 1407
LweI GCATC 1 cut(s) 1433
MaeI CTAG 2 cut(s) 825, 1044
MaeII ACGT 4 cut(s) 849, 942, 1026, 1401
MaeIII GTNAC 6 cut(s) 223, 694, 850, 1027, 1144, 1448
MalI GATC 6 cut(s) 321, 476, 504, 928, 1042, 1076
MbiI CCGCTC 1 cut(s) 383
MboI GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
MboII GAAGA 4 cut(s) 383, 1008, 1022, 1357
MfeI CAATTG 1 cut(s) 667
MflI RGATCY 4 cut(s) 474, 502, 926, 1040
MluCI AATT 7 cut(s) 137, 428, 667, 736, 775, 843, 1315
MlyI GAGTC 1 cut(s) 892
MmeI TCCRAC 2 cut(s) 399, 1047
MseI TTAA 5 cut(s) 39, 423, 789, 1245, 1314
MslI CAYNNNNRTG 2 cut(s) 327, 878
MspI CCGG 4 cut(s) 157, 165, 278, 1088
MspR9I CCNGG 6 cut(s) 157, 158, 591, 966, 1089, 1332
MunI CAATTG 1 cut(s) 667
Mva1269I GAATGC 1 cut(s) 1214
MvaI CCWGG 3 cut(s) 591, 966, 1332
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 8 cut(s) 111, 214, 450, 547, 689, 953, 966, 987
NciI CCSGG 3 cut(s) 157, 158, 1089
NcoI CCATGG 1 cut(s) 970
NdeII GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
NlaIII CATG 7 cut(s) 636, 764, 974, 1019, 1063, 1086, 1341
NlaIV GGNNCC 8 cut(s) 6, 153, 282, 399, 504, 595, 928, 1057
NmuCI GTSAC 2 cut(s) 223, 1144
NspI RCATGY 2 cut(s) 764, 1341
PauI GCGCGC 1 cut(s) 444
PctI GAATGC 1 cut(s) 1214
PfeI GAWTC 1 cut(s) 975
PflMI CCANNNNNTGG 1 cut(s) 971
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 6 cut(s) 402, 540, 663, 1322, 1387, 1397
PleI GAGTC 1 cut(s) 891
PpsI GAGTC 1 cut(s) 891
PpuMI RGGWCCY 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 3 cut(s) 589, 964, 1330
PspGI CCWGG 3 cut(s) 589, 964, 1330
PspN4I GGNNCC 8 cut(s) 6, 153, 282, 399, 504, 595, 928, 1057
PspPI GGNCC 3 cut(s) 167, 587, 962
PspPPI RGGWCCY 1 cut(s) 587
PstI CTGCAG 2 cut(s) 210, 1230
PsuI RGATCY 4 cut(s) 474, 502, 926, 1040
PteI GCGCGC 1 cut(s) 444
RsaI GTAC 5 cut(s) 58, 82, 128, 545, 1265
RsaNI GTAC 5 cut(s) 57, 81, 127, 544, 1264
RseI CAYNNNNRTG 2 cut(s) 327, 878
SaqAI TTAA 5 cut(s) 39, 423, 789, 1245, 1314
SatI GCNGC 6 cut(s) 401, 539, 662, 1321, 1386, 1396
Sau3AI GATC 6 cut(s) 319, 474, 502, 926, 1040, 1074
Sau96I GGNCC 3 cut(s) 167, 587, 962
ScaI AGTACT 3 cut(s) 58, 128, 545
SchI GAGTC 1 cut(s) 892
ScrFI CCNGG 6 cut(s) 157, 158, 591, 966, 1089, 1332
SfaNI GCATC 1 cut(s) 1433
SfcI CTRYAG 2 cut(s) 206, 1226
SinI GGWCC 3 cut(s) 167, 587, 962
SmaI CCCGGG 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 327, 878
SmlI CTYRAG 1 cut(s) 1370
SmoI CTYRAG 1 cut(s) 1370
Sse9I AATT 7 cut(s) 137, 428, 667, 736, 775, 843, 1315
SsiI CCGC 4 cut(s) 217, 383, 401, 960
SspMI CTAG 2 cut(s) 825, 1044
StyD4I CCNGG 6 cut(s) 155, 156, 589, 964, 1087, 1330
StyI CCWWGG 2 cut(s) 478, 970
TaaI ACNGT 3 cut(s) 724, 1033, 1285
TaiI ACGT 4 cut(s) 852, 945, 1029, 1404
TaqI TCGA 2 cut(s) 870, 1164
TasI AATT 7 cut(s) 137, 428, 667, 736, 775, 843, 1315
TatI WGTACW 3 cut(s) 56, 126, 543
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 1 cut(s) 975
Tru1I TTAA 5 cut(s) 39, 423, 789, 1245, 1314
Tru9I TTAA 5 cut(s) 39, 423, 789, 1245, 1314
TscAI CASTG 4 cut(s) 210, 841, 1189, 1330
TseFI GTSAC 2 cut(s) 223, 1144
TseI GCWGC 5 cut(s) 538, 661, 1320, 1385, 1395
Tsp45I GTSAC 2 cut(s) 223, 1144
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 4 cut(s) 393, 624, 797, 1218
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 4 cut(s) 210, 841, 1189, 1330
Van91I CCANNNNNTGG 1 cut(s) 971
VpaK11BI GGWCC 3 cut(s) 167, 587, 962
XagI CCTNNNNNAGG 1 cut(s) 559
XapI RAATTY 2 cut(s) 736, 843
XceI RCATGY 2 cut(s) 764, 1341
XcmI CCANNNNNNNNNTGG 2 cut(s) 382, 1274
XmaI CCCGGG 1 cut(s) 156
XspI CTAG 2 cut(s) 825, 1044
ZrmI AGTACT 3 cut(s) 58, 128, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.