Rroxscaffold_7G00181500

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016261
Physical Location & Seq
Forward (+)
20593009 .. 20595971
2963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00181500.1

Sequence Viewer

Length: 1458 bp
ATGGCTCCACAAAGACAATTATTCTCGTTCTGTATTTTTAGTGTCCTCTCCTTTAGTACTATAAGTTCAGGCTATGGTGGTACTTTGCCAGATAACAAACCAAACACTCAGCGCTTCGCTCCTAAGTACTCATATAAATTGGGTAAATCGGTTCCCCGGGAGGCCGGTTCTGAAAGGCTTTCCCTTCACACATTGGGTAGCACACTGCAATCAAAGTGGATGGTGACTAATCCTTTTCATCCCTTTCACAATGTGAGTGGCGCACGACCATCAACTCCGGCTCCGATTAGCGTTGATAAGTTTGGAGCTAAAGGGATTGATCATACAGATGACACAGAGGCATTTCAGAAGGCGTGGCTGGAAGCTTGTTCCTCCAACGGAGCGGTGGTTCTTCTGGTGCCGCCCCACAGGAGCTATTTCGTTAAGCAATTCACATTCTCAGGCGCGCAATGCAAATCTCATATTACAATGCAGATCCAAGGAACGATAATGGCATTTGAGGATCCGAAAACCTACCATTTTCCAAATAAAAGTAAGCAGCAGTACTGGCTTACCTTTGAGAGGGTTCAGAACTTGGTGGTTGAAGGTCCTGGAACCATCAATGGCAACGGAGAAAAATGGTGGCAAAGCTCATGCAAACAAAACAATATAACTCGCACGCTGGTGCCCAATTGCAATGACGACGCACCAACTGCTGTAACCTTCAAGAAGTGCAATAACTTGACGGTGCATAATCTGAATTTTGAAAAGGCACAGCAGAAGCATGTTTCTTTTGAAAATTGCACGGATGTTAAAGCTTCCCGTCTTAGAATAAATACATCAGAGGCTAGTCCCAACACTGATGGAATTCACGTTACAGAAACCAAGAACATCGACATTTCAATGAGTCGTATAAGCACAGGTGATGATTGTATATCCATTATGGGAGGATCCCGGAATGTTTACGTCGCAAACATAGCTTGCGGACCAGGCCATGGAATCAGCATTGGTAGCTTGGGAAAAGGTGGCTCAGAAGACCATGTTTCCGACGTAACAGTCTTTGGATCTAGGCTAACTGGAACCATGTATGGAGTGAGGATCAAGACATGGCCGGGTGGGTCAGGGTATGCAGCCAACATCACATTTCAGATGATACAGATGGATAATGTGACCAACCCCATAATCATCGACCAAAACTACTGCGACACTGCAACCAAAGTCATCAACGGAGAATGCAAACCACCACCACTGCAGAAAAATACAGCAGTTGAAGTGCATAATGTTATGTACCGGAGCATAAATGGGACAGTTTCTAAGGACGGCCAAGCCATAGTATTTAATTGCAGCAGTGTTCCTGGTGCATGTGAGAAGATTGAGCTGGACGATGTTAGACTTGAGAAAGGAGGAGCTGCTGTATGCAGCAACGTCAAACCGACTTACAGCAGAGTAGATCTGCCCCTCAATCGATGCCCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

485

Amino Acids

53.02

Weight (kDa)

8.7

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 123 - 471 3.4e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20980
rosa_chinensis RchiOBHm_Chr6g0287361
rosa_laevigata RLG00000012495
rosa_multiflora Rmu_sc0007029.1_g000028
rosa_roxburghii Rroxscaffold_7G00181500
rosa_rugosa Rorug06G0187300 Rorug06G0187400 Rorug06G0187500 Rorug06G0187600.1
rosa_samantha Rh6AG298800 Rh6BG304300 Rh6CG305300 Rh6CG312900 Rh6DG296200
rosa_wichuraiana Rw6G025820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1034
AccB1I GGYRCC 2 cut(s) 397, 664
AccB7I CCANNNNNTGG 1 cut(s) 974
AccBSI CCGCTC 1 cut(s) 383
AccII CGCG 1 cut(s) 446
AciI CCGC 3 cut(s) 383, 401, 963
AclWI GGATC 7 cut(s) 469, 497, 510, 924, 937, 1051, 1085
AcoI YGGCCR 2 cut(s) 1088, 1300
AcsI RAATTY 2 cut(s) 739, 846
AdeI CACNNNGTG 1 cut(s) 253
AfaI GTAC 5 cut(s) 58, 82, 128, 545, 1268
AfeI AGCGCT 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 561, 974
AgsI TTSAA 6 cut(s) 584, 706, 746, 776, 882, 1250
AjnI CCWGG 3 cut(s) 589, 967, 1333
AleI CACNNNNGTG 1 cut(s) 662
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 9 cut(s) 308, 365, 414, 630, 797, 959, 993, 1357, 1388
AluI AGCT 9 cut(s) 308, 365, 414, 630, 797, 959, 993, 1357, 1388
AlwI GGATC 7 cut(s) 469, 497, 510, 924, 937, 1051, 1085
Ama87I CYCGRG 1 cut(s) 156
Aor51HI AGCGCT 1 cut(s) 113
AoxI GGCC 4 cut(s) 162, 970, 1088, 1300
ApeKI GCWGC 5 cut(s) 538, 1109, 1323, 1388, 1398
ApoI RAATTY 2 cut(s) 739, 846
AspLEI GCGC 4 cut(s) 114, 263, 446, 448
AspS9I GGNCC 2 cut(s) 587, 965
AsuC2I CCSGG 4 cut(s) 157, 158, 934, 1092
AsuHPI GGTGA 2 cut(s) 235, 914
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 2 cut(s) 587, 965
BaeGI GKGCMC 1 cut(s) 669
BamHI GGATCC 2 cut(s) 502, 929
BanI GGYRCC 2 cut(s) 397, 664
BbsI GAAGAC 1 cut(s) 1020
BbvI GCAGC 5 cut(s) 550, 1121, 1335, 1375, 1410
BccI CCATC 5 cut(s) 214, 277, 605, 836, 1132
BceAI ACGGC 1 cut(s) 1315
BciT130I CCWGG 3 cut(s) 591, 969, 1335
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 4 cut(s) 157, 158, 934, 1092
BfaI CTAG 2 cut(s) 828, 1047
BfmI CTRYAG 1 cut(s) 1229
BfoI RGCGCY 1 cut(s) 115
BglII AGATCT 1 cut(s) 1429
BisI GCNGC 6 cut(s) 401, 539, 1110, 1324, 1389, 1399
BlsI GCNGC 6 cut(s) 402, 540, 1111, 1325, 1390, 1400
BmcAI AGTACT 3 cut(s) 58, 128, 545
Bme1390I CCNGG 7 cut(s) 157, 158, 591, 934, 969, 1092, 1335
Bme18I GGWCC 2 cut(s) 587, 965
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 2 cut(s) 587, 965
BmiI GGNNCC 9 cut(s) 6, 153, 282, 399, 504, 595, 666, 931, 1060
BmrFI CCNGG 7 cut(s) 157, 158, 591, 934, 969, 1092, 1335
BmsI GCATC 1 cut(s) 1436
BpiI GAAGAC 1 cut(s) 1020
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 4 cut(s) 157, 158, 934, 1092
Bsa29I ATCGAT 1 cut(s) 1444
BsaJI CCNNGG 4 cut(s) 155, 156, 478, 973
BsaWI WCCGGW 1 cut(s) 1269
BsaXI ACNNNNNCTCC 6 cut(s) 265, 295, 372, 402, 1377, 1407
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 974
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 551, 1060
Bse3DI GCAATG 2 cut(s) 455, 682
BseBI CCWGG 3 cut(s) 591, 969, 1335
BseCI ATCGAT 1 cut(s) 1444
BseDI CCNNGG 4 cut(s) 155, 156, 478, 973
BseGI GGATG 3 cut(s) 225, 238, 793
BseLI CCNNNNNNNGG 2 cut(s) 561, 974
BseMI GCAATG 2 cut(s) 455, 682
BseMII CTCAG 3 cut(s) 122, 453, 1023
BseNI ACTGG 2 cut(s) 551, 1060
BsePI GCGCGC 1 cut(s) 444
BseRI GAGGAG 1 cut(s) 1398
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 5 cut(s) 550, 1121, 1335, 1375, 1410
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 4 cut(s) 164, 972, 1090, 1302
BshNI GGYRCC 2 cut(s) 397, 664
BshVI ATCGAT 1 cut(s) 1444
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 6 cut(s) 157, 165, 278, 934, 1091, 1270
BslFI GGGAC 2 cut(s) 816, 1297
BslI CCNNNNNNNGG 2 cut(s) 561, 974
BsmFI GGGAC 2 cut(s) 816, 1297
BsmI GAATGC 1 cut(s) 1217
BsnI GGCC 4 cut(s) 164, 972, 1090, 1302
BsoBI CYCGRG 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
Bsp19I CCATGG 1 cut(s) 973
BspACI CCGC 3 cut(s) 383, 401, 963
BspANI GGCC 4 cut(s) 164, 972, 1090, 1302
BspCNI CTCAG 3 cut(s) 121, 452, 1022
BspDI ATCGAT 1 cut(s) 1444
BspFNI CGCG 1 cut(s) 446
BspLI GGNNCC 9 cut(s) 6, 153, 282, 399, 504, 595, 666, 931, 1060
BspMAI CTGCAG 1 cut(s) 1233
BspPI GGATC 7 cut(s) 469, 497, 510, 924, 937, 1051, 1085
BspT107I GGYRCC 2 cut(s) 397, 664
BsrBI CCGCTC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 455, 682
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 2 cut(s) 551, 1060
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 155, 156, 478, 973
BssHII GCGCGC 1 cut(s) 444
BssMI GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
BssT1I CCWWGG 2 cut(s) 478, 973
Bst2UI CCWGG 3 cut(s) 591, 969, 1335
Bst4CI ACNGT 3 cut(s) 727, 1036, 1288
BstAPI GCANNNNNTGC 1 cut(s) 692
BstC8I GCNNGC 3 cut(s) 446, 659, 961
BstDEI CTNAG 6 cut(s) 108, 123, 439, 806, 1009, 1293
BstDSI CCRYGG 1 cut(s) 973
BstENI CCTNNNNNAGG 1 cut(s) 559
BstF5I GGATG 3 cut(s) 225, 238, 793
BstFNI CGCG 1 cut(s) 446
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 4 cut(s) 114, 263, 446, 448
BstKTI GATC 7 cut(s) 322, 477, 505, 932, 1046, 1080, 1432
BstMBI GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
BstMWI GCNNNNNNNGC 6 cut(s) 450, 547, 692, 956, 969, 990
BstNI CCWGG 3 cut(s) 591, 969, 1335
BstNSI RCATGY 2 cut(s) 767, 1344
BstSCI CCNGG 7 cut(s) 155, 156, 589, 932, 967, 1090, 1333
BstSFI CTRYAG 1 cut(s) 1229
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 5 cut(s) 550, 1121, 1335, 1375, 1410
BstV2I GAAGAC 1 cut(s) 1020
BstX2I RGATCY 5 cut(s) 474, 502, 929, 1043, 1429
BstYI RGATCY 5 cut(s) 474, 502, 929, 1043, 1429
Bsu15I ATCGAT 1 cut(s) 1444
BsuRI GGCC 4 cut(s) 164, 972, 1090, 1302
BsuTUI ATCGAT 1 cut(s) 1444
BtgI CCRYGG 1 cut(s) 973
BtsCI GGATG 3 cut(s) 225, 238, 793
BtsI GCAGTG 4 cut(s) 203, 1185, 1226, 1333
BtsIMutI CAGTG 5 cut(s) 203, 837, 1185, 1226, 1333
Cac8I GCNNGC 3 cut(s) 446, 659, 961
CfoI GCGC 4 cut(s) 114, 263, 446, 448
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 587, 965
Cfr9I CCCGGG 1 cut(s) 156
ClaI ATCGAT 1 cut(s) 1444
CseI GACGC 1 cut(s) 692
Csp6I GTAC 5 cut(s) 57, 81, 127, 544, 1267
CspCI CAANNNNNGTGG 4 cut(s) 197, 232, 238, 273
CviAII CATG 7 cut(s) 633, 764, 974, 1019, 1063, 1086, 1341
CviQI GTAC 5 cut(s) 57, 81, 127, 544, 1267
DdeI CTNAG 6 cut(s) 108, 123, 439, 806, 1009, 1293
DpnI GATC 7 cut(s) 321, 476, 504, 931, 1045, 1079, 1431
DpnII GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
DraIII CACNNNGTG 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 1034
DseDI GACNNNNNNGTC 1 cut(s) 1034
EaeI YGGCCR 2 cut(s) 1088, 1300
Eco130I CCWWGG 2 cut(s) 478, 973
Eco47I GGWCC 2 cut(s) 587, 965
Eco47III AGCGCT 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 156
EcoNI CCTNNNNNAGG 1 cut(s) 559
EcoO109I RGGNCCY 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 846
EcoRII CCWGG 3 cut(s) 589, 967, 1333
EcoT14I CCWWGG 2 cut(s) 478, 973
ErhI CCWWGG 2 cut(s) 478, 973
FaeI CATG 7 cut(s) 636, 767, 977, 1022, 1066, 1089, 1344
FaqI GGGAC 2 cut(s) 816, 1297
FatI CATG 7 cut(s) 632, 763, 973, 1018, 1062, 1085, 1340
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 6 cut(s) 401, 539, 1110, 1324, 1389, 1399
FokI GGATG 3 cut(s) 225, 232, 800
Fsp4HI GCNGC 6 cut(s) 401, 539, 1110, 1324, 1389, 1399
FspBI CTAG 2 cut(s) 828, 1047
GlaI GCGC 4 cut(s) 113, 262, 445, 447
GluI GCNGC 6 cut(s) 401, 539, 1110, 1324, 1389, 1399
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 4 cut(s) 164, 972, 1090, 1302
HapII CCGG 6 cut(s) 157, 165, 278, 934, 1091, 1270
HgaI GACGC 1 cut(s) 692
HhaI GCGC 4 cut(s) 114, 263, 446, 448
Hin1II CATG 7 cut(s) 636, 767, 977, 1022, 1066, 1089, 1344
Hin6I GCGC 4 cut(s) 112, 261, 444, 446
HinP1I GCGC 4 cut(s) 112, 261, 444, 446
HindIII AAGCTT 2 cut(s) 363, 795
HinfI GANTC 2 cut(s) 886, 978
HpaII CCGG 6 cut(s) 157, 165, 278, 934, 1091, 1270
HphI GGTGA 2 cut(s) 235, 914
Hpy166II GTNNAC 1 cut(s) 943
Hpy188III TCNNGA 2 cut(s) 706, 1081
Hpy8I GTNNAC 1 cut(s) 943
Hpy99I CGWCG 3 cut(s) 686, 950, 1031
HpyAV CCTTC 4 cut(s) 194, 343, 578, 712
HpyCH4III ACNGT 3 cut(s) 727, 1036, 1288
HpyCH4IV ACGT 4 cut(s) 852, 945, 1029, 1404
HpyF10VI GCNNNNNNNGC 6 cut(s) 450, 547, 692, 956, 969, 990
HpyF3I CTNAG 6 cut(s) 108, 123, 439, 806, 1009, 1293
HpySE526I ACGT 4 cut(s) 852, 945, 1029, 1404
Hsp92II CATG 7 cut(s) 636, 767, 977, 1022, 1066, 1089, 1344
HspAI GCGC 4 cut(s) 112, 261, 444, 446
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
LmnI GCTCC 8 cut(s) 10, 124, 286, 305, 380, 411, 1272, 1385
Lsp1109I GCAGC 5 cut(s) 550, 1121, 1335, 1375, 1410
LweI GCATC 1 cut(s) 1436
MaeI CTAG 2 cut(s) 828, 1047
MaeII ACGT 4 cut(s) 852, 945, 1029, 1404
MaeIII GTNAC 5 cut(s) 223, 697, 853, 1030, 1147
MalI GATC 7 cut(s) 321, 476, 504, 931, 1045, 1079, 1431
MbiI CCGCTC 1 cut(s) 383
MboI GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
MboII GAAGA 3 cut(s) 383, 1025, 1360
MfeI CAATTG 1 cut(s) 670
MflI RGATCY 5 cut(s) 474, 502, 929, 1043, 1429
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 8 cut(s) 17, 137, 428, 670, 739, 778, 846, 1318
MlyI GAGTC 1 cut(s) 895
MmeI TCCRAC 2 cut(s) 399, 1050
MseI TTAA 3 cut(s) 423, 792, 1317
MslI CAYNNNNRTG 3 cut(s) 327, 662, 881
MspI CCGG 6 cut(s) 157, 165, 278, 934, 1091, 1270
MspR9I CCNGG 7 cut(s) 157, 158, 591, 934, 969, 1092, 1335
MunI CAATTG 1 cut(s) 670
Mva1269I GAATGC 1 cut(s) 1217
MvaI CCWGG 3 cut(s) 591, 969, 1335
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 6 cut(s) 450, 547, 692, 956, 969, 990
NciI CCSGG 4 cut(s) 157, 158, 934, 1092
NcoI CCATGG 1 cut(s) 973
NdeII GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
NlaIII CATG 7 cut(s) 636, 767, 977, 1022, 1066, 1089, 1344
NlaIV GGNNCC 9 cut(s) 6, 153, 282, 399, 504, 595, 666, 931, 1060
NmuCI GTSAC 2 cut(s) 223, 1147
NspI RCATGY 2 cut(s) 767, 1344
OliI CACNNNNGTG 1 cut(s) 662
PauI GCGCGC 1 cut(s) 444
PctI GAATGC 1 cut(s) 1217
PfeI GAWTC 1 cut(s) 978
PflMI CCANNNNNTGG 1 cut(s) 974
PfoI TCCNGGA 2 cut(s) 589, 932
PkrI GCNGC 6 cut(s) 402, 540, 1111, 1325, 1390, 1400
PleI GAGTC 1 cut(s) 894
PpsI GAGTC 1 cut(s) 894
PpuMI RGGWCCY 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 3 cut(s) 589, 967, 1333
PspGI CCWGG 3 cut(s) 589, 967, 1333
PspN4I GGNNCC 9 cut(s) 6, 153, 282, 399, 504, 595, 666, 931, 1060
PspPI GGNCC 2 cut(s) 587, 965
PspPPI RGGWCCY 1 cut(s) 587
PsrI GAACNNNNNNTAC 2 cut(s) 49, 81
PstI CTGCAG 1 cut(s) 1233
PsuI RGATCY 5 cut(s) 474, 502, 929, 1043, 1429
PteI GCGCGC 1 cut(s) 444
RsaI GTAC 5 cut(s) 58, 82, 128, 545, 1268
RsaNI GTAC 5 cut(s) 57, 81, 127, 544, 1267
RseI CAYNNNNRTG 3 cut(s) 327, 662, 881
SaqAI TTAA 3 cut(s) 423, 792, 1317
SatI GCNGC 6 cut(s) 401, 539, 1110, 1324, 1389, 1399
Sau3AI GATC 7 cut(s) 319, 474, 502, 929, 1043, 1077, 1429
Sau96I GGNCC 2 cut(s) 587, 965
ScaI AGTACT 3 cut(s) 58, 128, 545
SchI GAGTC 1 cut(s) 895
ScrFI CCNGG 7 cut(s) 157, 158, 591, 934, 969, 1092, 1335
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 1 cut(s) 1436
SfcI CTRYAG 1 cut(s) 1229
SinI GGWCC 2 cut(s) 587, 965
SmaI CCCGGG 1 cut(s) 158
SmiMI CAYNNNNRTG 3 cut(s) 327, 662, 881
SmlI CTYRAG 1 cut(s) 1373
SmoI CTYRAG 1 cut(s) 1373
Sse9I AATT 8 cut(s) 17, 137, 428, 670, 739, 778, 846, 1318
SsiI CCGC 3 cut(s) 383, 401, 963
SspMI CTAG 2 cut(s) 828, 1047
StyD4I CCNGG 7 cut(s) 155, 156, 589, 932, 967, 1090, 1333
StyI CCWWGG 2 cut(s) 478, 973
TaaI ACNGT 3 cut(s) 727, 1036, 1288
TaiI ACGT 4 cut(s) 855, 948, 1032, 1407
TaqI TCGA 3 cut(s) 873, 1167, 1444
TasI AATT 8 cut(s) 17, 137, 428, 670, 739, 778, 846, 1318
TatI WGTACW 3 cut(s) 56, 126, 543
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 1 cut(s) 978
Tru1I TTAA 3 cut(s) 423, 792, 1317
Tru9I TTAA 3 cut(s) 423, 792, 1317
TscAI CASTG 5 cut(s) 210, 844, 1192, 1233, 1333
TseFI GTSAC 2 cut(s) 223, 1147
TseI GCWGC 5 cut(s) 538, 1109, 1323, 1388, 1398
Tsp45I GTSAC 2 cut(s) 223, 1147
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 4 cut(s) 393, 624, 800, 1221
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 5 cut(s) 210, 844, 1192, 1233, 1333
Van91I CCANNNNNTGG 1 cut(s) 974
VpaK11BI GGWCC 2 cut(s) 587, 965
XagI CCTNNNNNAGG 1 cut(s) 559
XapI RAATTY 2 cut(s) 739, 846
XceI RCATGY 2 cut(s) 767, 1344
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XmaI CCCGGG 1 cut(s) 156
XspI CTAG 2 cut(s) 828, 1047
ZrmI AGTACT 3 cut(s) 58, 128, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.