Rmu_sc0007029.1_g000028

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007029.1
OG0016261
Physical Location & Seq
Forward (+)
126224 .. 127718
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007029.1_g000028.1.cds

Sequence Viewer

Length: 765 bp
atgcaggctgtaaccttcaagacgtgcaataacttgacggtgcataatctgaattttgaaaaggcacagcagaagcatgtttcgtttgaaaattgcacggatgttaaagcttcccgtcttagaataaatacaacagaggctagtcccaacactgatggaattcacgttacagaaaccaagaacatcgacatttcaatgagtcgtataagcacaggtgatgattgtatatccattatgggaggatcccagaatgtttacgtcgcaaacatagcttgcggaccaggccatggaatcagcttgggaagaggtggctcagaagaccatgtttccgacgtaacagtctttggatcaaggctaactggaaccatgtatggagtgaggatcaagacatggccgggagggtcagggtatgcaaccaacatcacatttcagatgatacagatggataatgtgaccaaccccataatcatcgaccaaaactactgcgacactgcaaccaaagtcatcaacggagaatgcaaaccaccaccactgcagaaaaatacagcagttaaagtgcataatgttatgtaccagagcataaatgggacagtttctaaggacggccaagccatagtatttaattgcagcagtgttcctggtggtgcatgtgagaagattgtgctggacgatgttagacttgagaaaggaggagctgctgtatgcagcaacgtcaaaccgacttacagcagagtagatttgcccctcaatcgatgccctaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

254

Amino Acids

27.32

Weight (kDa)

7.53

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016261)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20980
rosa_chinensis RchiOBHm_Chr6g0287361
rosa_laevigata RLG00000012495
rosa_multiflora Rmu_sc0007029.1_g000028
rosa_roxburghii Rroxscaffold_7G00181500
rosa_rugosa Rorug06G0187300 Rorug06G0187400 Rorug06G0187500 Rorug06G0187600.1
rosa_samantha Rh6AG298800 Rh6BG304300 Rh6CG305300 Rh6CG312900 Rh6DG296200
rosa_wichuraiana Rw6G025820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 287
AciI CCGC 1 cut(s) 276
AclWI GGATC 4 cut(s) 237, 250, 355, 389
AcoI YGGCCR 2 cut(s) 392, 604
AcsI RAATTY 2 cut(s) 52, 159
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 4 cut(s) 19, 59, 89, 195
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 2 cut(s) 280, 637
AluBI AGCT 4 cut(s) 110, 272, 297, 695
AluI AGCT 4 cut(s) 110, 272, 297, 695
AlwI GGATC 4 cut(s) 237, 250, 355, 389
AoxI GGCC 3 cut(s) 283, 392, 604
ApeKI GCWGC 3 cut(s) 627, 695, 705
ApoI RAATTY 2 cut(s) 52, 159
AspS9I GGNCC 1 cut(s) 278
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 1 cut(s) 227
AvaII GGWCC 1 cut(s) 278
BamHI GGATCC 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 3 cut(s) 639, 682, 717
BccI CCATC 2 cut(s) 149, 436
BceAI ACGGC 1 cut(s) 619
BciT130I CCWGG 2 cut(s) 282, 639
BcnI CCSGG 1 cut(s) 396
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 533
BisI GCNGC 3 cut(s) 628, 696, 706
BlsI GCNGC 3 cut(s) 629, 697, 707
Bme1390I CCNGG 3 cut(s) 282, 396, 639
Bme18I GGWCC 1 cut(s) 278
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 244, 364
BmrFI CCNGG 3 cut(s) 282, 396, 639
BmsI GCATC 1 cut(s) 743
BpiI GAAGAC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 396
Bsa29I ATCGAT 1 cut(s) 751
BsaJI CCNNGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 364
BseBI CCWGG 2 cut(s) 282, 639
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 364
BseRI GAGGAG 1 cut(s) 705
BseXI GCAGC 3 cut(s) 639, 682, 717
BshFI GGCC 3 cut(s) 285, 394, 606
BshVI ATCGAT 1 cut(s) 751
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 2 cut(s) 129, 601
BslI CCNNNNNNNGG 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 129, 601
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 3 cut(s) 285, 394, 606
Bsp143I GATC 3 cut(s) 242, 347, 381
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 1 cut(s) 276
BspANI GGCC 3 cut(s) 285, 394, 606
BspCNI CTCAG 1 cut(s) 326
BspDI ATCGAT 1 cut(s) 751
BspLI GGNNCC 2 cut(s) 244, 364
BspMAI CTGCAG 1 cut(s) 537
BspPI GGATC 4 cut(s) 237, 250, 355, 389
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 3 cut(s) 242, 347, 381
BssT1I CCWWGG 1 cut(s) 286
Bst2UI CCWGG 2 cut(s) 282, 639
Bst4CI ACNGT 3 cut(s) 40, 340, 592
Bst6I CTCTTC 1 cut(s) 298
BstC8I GCNNGC 2 cut(s) 6, 274
BstDEI CTNAG 3 cut(s) 119, 313, 597
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 3 cut(s) 245, 350, 384
BstMBI GATC 3 cut(s) 242, 347, 381
BstMWI GCNNNNNNNGC 2 cut(s) 269, 282
BstNI CCWGG 2 cut(s) 282, 639
BstNSI RCATGY 2 cut(s) 80, 651
BstSCI CCNGG 3 cut(s) 280, 394, 637
BstSFI CTRYAG 1 cut(s) 533
BstV1I GCAGC 3 cut(s) 639, 682, 717
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 751
BsuRI GGCC 3 cut(s) 285, 394, 606
BsuTUI ATCGAT 1 cut(s) 751
BtgI CCRYGG 1 cut(s) 286
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 1 cut(s) 106
BtsI GCAGTG 3 cut(s) 489, 530, 637
BtsIMutI CAGTG 4 cut(s) 150, 489, 530, 637
Cac8I GCNNGC 2 cut(s) 6, 274
Cfr13I GGNCC 1 cut(s) 278
ClaI ATCGAT 1 cut(s) 751
Csp6I GTAC 1 cut(s) 571
CviAII CATG 6 cut(s) 77, 287, 323, 367, 390, 648
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 3 cut(s) 119, 313, 597
DpnI GATC 3 cut(s) 244, 349, 383
DpnII GATC 3 cut(s) 242, 347, 381
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 2 cut(s) 392, 604
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 278
EcoRI GAATTC 1 cut(s) 159
EcoRII CCWGG 2 cut(s) 280, 637
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 6 cut(s) 80, 290, 326, 370, 393, 651
FaqI GGGAC 2 cut(s) 129, 601
FatI CATG 6 cut(s) 76, 286, 322, 366, 389, 647
Fnu4HI GCNGC 3 cut(s) 628, 696, 706
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 3 cut(s) 628, 696, 706
FspBI CTAG 1 cut(s) 141
GluI GCNGC 3 cut(s) 628, 696, 706
HaeIII GGCC 3 cut(s) 285, 394, 606
HapII CCGG 1 cut(s) 395
Hin1II CATG 6 cut(s) 80, 290, 326, 370, 393, 651
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 2 cut(s) 199, 291
HpaII CCGG 1 cut(s) 395
HphI GGTGA 1 cut(s) 227
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 4 cut(s) 51, 316, 331, 432
Hpy188III TCNNGA 2 cut(s) 19, 385
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 2 cut(s) 263, 335
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 3 cut(s) 40, 340, 592
HpyCH4IV ACGT 5 cut(s) 23, 165, 258, 333, 711
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 282
HpyF3I CTNAG 3 cut(s) 119, 313, 597
HpySE526I ACGT 5 cut(s) 23, 165, 258, 333, 711
Hsp92II CATG 6 cut(s) 80, 290, 326, 370, 393, 651
Kzo9I GATC 3 cut(s) 242, 347, 381
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 3 cut(s) 639, 682, 717
LweI GCATC 1 cut(s) 743
MaeI CTAG 1 cut(s) 141
MaeII ACGT 5 cut(s) 23, 165, 258, 333, 711
MaeIII GTNAC 4 cut(s) 10, 166, 334, 451
MalI GATC 3 cut(s) 244, 349, 383
MboI GATC 3 cut(s) 242, 347, 381
MboII GAAGA 3 cut(s) 315, 329, 667
MflI RGATCY 1 cut(s) 242
MluCI AATT 4 cut(s) 52, 91, 159, 622
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 7 cut(s) 130, 233, 299, 372, 392, 683, 755
MseI TTAA 3 cut(s) 105, 552, 621
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 3 cut(s) 282, 396, 639
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 2 cut(s) 282, 639
MwoI GCNNNNNNNGC 2 cut(s) 269, 282
NciI CCSGG 1 cut(s) 396
NcoI CCATGG 1 cut(s) 286
NdeII GATC 3 cut(s) 242, 347, 381
NlaIII CATG 6 cut(s) 80, 290, 326, 370, 393, 651
NlaIV GGNNCC 2 cut(s) 244, 364
NmuCI GTSAC 1 cut(s) 451
NspI RCATGY 2 cut(s) 80, 651
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 291
PflMI CCANNNNNTGG 1 cut(s) 287
PkrI GCNGC 3 cut(s) 629, 697, 707
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 280, 637
PspGI CCWGG 2 cut(s) 280, 637
PspN4I GGNNCC 2 cut(s) 244, 364
PspPI GGNCC 1 cut(s) 278
PstI CTGCAG 1 cut(s) 537
PsuI RGATCY 1 cut(s) 242
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 3 cut(s) 105, 552, 621
SatI GCNGC 3 cut(s) 628, 696, 706
Sau3AI GATC 3 cut(s) 242, 347, 381
Sau96I GGNCC 1 cut(s) 278
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 3 cut(s) 282, 396, 639
SfaNI GCATC 1 cut(s) 743
SfcI CTRYAG 1 cut(s) 533
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 4 cut(s) 52, 91, 159, 622
SsiI CCGC 1 cut(s) 276
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 280, 394, 637
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 3 cut(s) 40, 340, 592
TaiI ACGT 5 cut(s) 26, 168, 261, 336, 714
TaqI TCGA 3 cut(s) 186, 471, 751
TasI AATT 4 cut(s) 52, 91, 159, 622
TfiI GAWTC 1 cut(s) 291
Tru1I TTAA 3 cut(s) 105, 552, 621
Tru9I TTAA 3 cut(s) 105, 552, 621
TscAI CASTG 4 cut(s) 157, 496, 537, 637
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 3 cut(s) 627, 695, 705
Tsp45I GTSAC 1 cut(s) 451
TspGWI ACGGA 2 cut(s) 113, 525
TspRI CASTG 4 cut(s) 157, 496, 537, 637
Van91I CCANNNNNTGG 1 cut(s) 287
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 2 cut(s) 52, 159
XceI RCATGY 2 cut(s) 80, 651
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.