RLG00000013028

Glycosyl transferase family 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007054
Physical Location & Seq
Forward (+)
25121685 .. 25135645
13961 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013028

Sequence Viewer

Length: 4434 bp
ATGGGGAATTGCTGGTTTTCTTCTTCAGCTGAAGACCCAACACCAACTACTACTGGTCATCTCAGTACAGTGCCGAGTAGCATATCTCAATCAATTAGTAATGCCACATCCACTTCCGTGGGGAGCACTATATCAAGGAATAGCCAGTTTTCGGAGAGAAGTGGTGATGAGACCTGTCCAGATGGGCAGATCCTACCCGCTGCAAACTTGAGGATCTTCAGTTACCTAGAATTGAAGAGTGCAACTAGAAACTTCAGATCAGACACGGTGCTTGGGGAAGGAGGTTTTGGGAAGGTCTATAAAGGCTGGCTCGAGGACAGGGCACCGACCAAGTCTGGAAAGACAACTGTAATTGCTGTAAAGAAATTGAATTCAGAGAGCTTGCAAGGTTTTGAGGAATGGCAGTCAGAGGTGAATTTCTTGGGGCGACTTTCTCATCCTAATCTTGTAAAGCTGCTGGGGTACTGTTGGGAGGAGAAAGAACTGCTGCTTATCTACGAGTTCATGCAAAAAGGAAGCTTGGAAAACCATCTTTTTGGAAGGGGTTCAGTTGTTCAGCCACTTCCTTGGGAAATACGGCTTAAAGTTGCAATAGGAGCAGCTCGAGGCCTAGCATTTTTGCATACATCAGAGAAGCAAGTAATCTATAGAGATTTCAAAGCTTCCAATATACTACTTGACGGGTCCTTTAACGCCAAGATATCCGACTTTGGCTTGGCAAAATTTGGTCCTTCAGAGAGTCAATCACACGTGACAACCCGGGTCATGGGCACATATGGTTACGCTGCTCCTGAGTATGTTGCCACAGGGCATCTCTATGTGAAGAGTGATGTGTACGGTTTTGGTGTTGTGTTGGTTGAGATCTTAACAGGCTTACGGGCACTTGATACGAATCGTCCAAGTGGAAAACATAATCTTGTGGACTGGGTAAAACCATATTTATCAGATAAAAGAAAATTGAAAGGCATTATGGACCCTCGACTGGAGGGAAAGTATCCAGCCAAAGCTGCATTCCGAATTTCTCAGCTTGCTCTAAAATGCATTGAATCTGAACACAAAAACCGGCCGCCAATGAAGGTCGTTGTGGAGACTTTGGAAAGGATCGAATCTGTCAATGAACATATAAGGGAGCCTAGGATTCGTTCGACTCTTTCTACTCATCCTATGGCTCATCGCCAAGGTCAGGCACCTTTGCACCATCGTTCTCCACTTCACCCCAGGTCAGATGGAAATCAAAACTACCAACAGTCACCTCGAATGAGATTGGGTATGGAAGTTGTTCCTACAACACCAGCGAGCTTCCACTCTCTGCTCTTCCTCTACTGCATCTCATTGGTTGTTTTTCATACCTTACTTGTTGGAGCTGATGTATCCCATGTTTCCACAATTCAGAGAGATGTCAGCAAGTGCCAGAGGTTGTGTCAATGTGAGCAATGCTGGCATACCAAGGAAGAAGGTGAATCTTATGTAACCGGAACCAGCAAAGTTCTGGATGAAAAGAATATTGACATAATTGCGACATACAGCAACAATTATGGAGCTGTTCAGAGTAGTAGGGTTAGACTGGGAGACCTATCTGCTTCCTGGGTCTTGGAAAATCCCATTGATGGAAGGCATGGCCATCCAAAGAGTGTGCAGATAGTGGAGGATTCATTTCAATCTGGAATGAGAGTTAAAGAGAACAGTGAGCATTCTACAGATGATCCTCATTCTGGAGAAGGTGAACTCCCGTATTCTCATCTATCATCACTCAGCCCAGTGAAGCACAAACGACGGATAATGCGAGAAGAAAGGAGGAAACTTCGGACTGCAGAGCTAATGGGAAAAGATAAAGAAGCTAATGACCAGATGGCAGCAGCAGCAATTGACCAATCAAAATCCTTCGACACCACTGTCACGGGAAAGTACAGCATATGGAGGAGAGATTATGAAAACCCAAATTCTGATTCAACCTTGAAACTTATGAGGGACCAGATCATAATGGCCATTGCTTATGCAAACATTGCCAAGTCAAAGAACGAAAGTGTTCTTTATAAATCTCTCATGAAGCACTCTAAGCTTAGTCAGAATGCTATTGGAGAAGCACATTCCGATGCTGAGCTTCCTTCAAGTGCACTTAAACGAGCAAAAGCAATGGGCCGTGCTCTCTCTGTAGCAAAGGACAAACTATATGACTGTCATACAATGGAGACGAAGTTAAGAGCTATGCTTCAGTCAAGTGAAGATAATGTAAATGGTCTGAAGAAAAAGAGTGCATTTTTGATCCAGCTTGCGGCAAAAACAGTCCCCAAACCGTTACATTGCCTTCCTTTGCAACTTGCATCAGACTATTTCCTGTTGGGCTATAATAATAGGGAGGATGTTAACAAAGAAAAGCTTGAGGATCCTTCTCTGTACCACTATGCTATCTTTTCTGACAATGTTCTAGCAACATCAGTGGTCGTTAATTCTACTGTGTTGCATGCAAAGGAACCCAGCAAGCATGTTTTCCATGTGGTAACTGATAAATTAAACTATGCTGCTATGAAAATGTGGTTTCTTGTCAACCCTCCTGCTGGAGCAGCAGTGCAGGTTGAAAATATTGATGATTTTAAGTGGCTAAATTCCTCTTCTTGTTCTGTACTACGTCAACTAGAGTCTGCCAGACTTCAAGAGTATTATTTCAAGGCAAACCATCCTTCTTCACTATCACTGGGTTCTGACCAGCTCAAATATCGGAATCCAAAATATTTATCACTGCTGAATCATCTGAGATTCTACCTTCCTGACGTTTACCCAAAGTTGGACAAGATCCTCTTTTTGGATGATGACATTGTAGTTCAGAAGGATTTGACAGCTCTTTGGTCTATTGATCTTCAAGGGATGGTAAATGGTGCAGTGGAGACCTGTAAAGAGAGCTTCCATAGGTATGATAAATACCTGAACTTCTCTAATCCAATAATATCTGCGAATTTCGATCCAAATGCTTGTGGCTGGGCATTCGGCATGAATATGTTTGACTTGAAAGAGTGGAGGAAGCGAAACATGACCGGGGTATATCATAAGTGGCAAGATATGAATGAGGATCGAACTCTTTGGAAACTTGGTACATTGCCACCAGGACTTATTACATTCTATAAGCTGACCTATCCGCTGGATCGTGGATGGCATTCTTTGGGACTTGGCTATGACCCAGCCCTTAACCAAACGGCAATAGAGAATGCAGCAGTCATCCATTACAATGGAAACTACAAGCCATGGTTGGATTTGGCTATTTCTAAGTACAAGAATTACTGGTCAAAATATGTAATTTCAAAACTCTTCTTCGAGTCCGTATGCAGATATGACATGGAAAGTGCACAGAAGTCGTTTGTGAAATGCCTGAGAGACATGTTATCTCTGGGAGGTTATGTGCACTGTGAGGAAGCTCAAATTGGCAATAAGATGTTCCAAAACACGGTATGTAAGCTTGACATAGAAACATGTGAGATTTCAAAGAGTTTTGCTTCCAAGGCTTTGATTTATGAGGTCAATGACAGGCCAGCTTTGGAGGCCGATCCTCATCTCTATGACCATAGAGTGTTGGTCCAAAGCAAGAGCTTTTCTTTTGTCACCACCTCAGATCCGATCCTGTGCACAATCTCTTCTATTGTTCGTCTGAAGTCAACAACGCATGCGCCACCTTCGATGAGCGTTGCTTTAAGCGGTTACCCGCCAGTGCACACCGGGTTCTACCCATTCGTGCAACAAAGATTGCCAACCGGCGACCTTTTGGTTGCTGTCTTTGTGGATCTGTTTGGTTTTCCTTATCTTCTGTTTTTCTTGGGTCAGGCTGTGTATTTGGAATTGGAGAATTGTGTTTCTGTGTTCCCAAGCACCTTGGATCCGCTTGATCCAAGCACTGCCTTGGGAGGATTCTCTGGTAACAAATATTCTGAGGCTTCAGCATTCGTAGTCACCTACCCAGTTAATAATGCAGTTGATGATGTAGGAAATGAGAATGGGAAGGCACTGGCTTGGGAGAAGGCTTTTATTCAGTTAGCAAAGGATGAACTGCTGCCAATGGTGCTGTCTCGGAATCTGAGTCTCTCATTTTCGGCTGAAACCTCAACTGAGGAAGAATTAAAAAGAGAAAGTACTGCAGATGTTATGACCATAATAATGCACCTCAAATATCTTCTTTTTACCTCTCGTCCAAGGTACGATCTTATCCTTCACCTGAGAGTATTGCTTGTAATGCTTTCTGTTCTTGGATCTGTTGGATTTTTCAGTGCTGTGAGAGTTAAATCTACATTAATCATAATGGAAGTCATTCCTTGCCTTGTTTTGGCTGTTGGTGTAGATAACATGTGTATATTGGTACATGCAATCACCGGAGTTACCTTTAGAATGACGATGGGAAGCTTCATTCCTATGCCAGCATGCAGAGTCTTCTCCATGTTTGCAGGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_8 Pkinase_Tyr

Protein Analysis

1478

Amino Acids

165.8

Weight (kDa)

8.25

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 87 - 362 3.9e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 88 - 365 6.9e-46 Protein tyrosine and serine/threonine kinase
GAUT_1 PF25557 648 - 724 2.5e-26 Galacturonosyltransferase domain 1
Glyco_transf_8 PF01501 775 - 1083 2.6e-79 Glycosyl transferase family 8
NPC1_MLD PF22314 1288 - 1361 1.4e-11 NPC1, middle luminal domain
Sterol-sensing PF12349 1401 - 1452 2.7e-09 Sterol-sensing domain of SREBP cleavage-activation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2034
AarI CACCTGC 1 cut(s) 4415
AasI GACNNNNNNGTC 1 cut(s) 1892
Acc36I ACCTGC 2 cut(s) 2560, 4415
AccB1I GGYRCC 2 cut(s) 322, 1186
AciI CCGC 7 cut(s) 198, 1067, 2273, 3131, 3684, 3692, 3866
AcoI YGGCCR 3 cut(s) 1064, 1618, 1983
AcsI RAATTY 7 cut(s) 370, 415, 722, 1017, 1939, 2602, 2950
AcuI CTGAAG 9 cut(s) 9, 51, 202, 238, 717, 2195, 2261, 3659, 3906
AcvI CACGTG 1 cut(s) 751
AflIII ACRYGT 4 cut(s) 748, 3369, 3461, 4326
AjnI CCWGG 3 cut(s) 1217, 1583, 3097
AjuI GAANNNNNNNTTGG 8 cut(s) 270, 302, 995, 1027, 1930, 1962, 3396, 3428
AleI CACNNNNGTG 1 cut(s) 116
Alw21I GWGCWC 7 cut(s) 128, 2116, 2146, 3340, 3396, 3617, 3702
Alw26I GTCTC 8 cut(s) 164, 1082, 1563, 2183, 2876, 3360, 4056, 4070
Alw44I GTGCAC 5 cut(s) 2112, 3336, 3392, 3613, 3698
Ama87I CYCGRG 3 cut(s) 311, 603, 759
AoxI GGCC 7 cut(s) 607, 1064, 1618, 1983, 2137, 3518, 3531
ApaLI GTGCAC 5 cut(s) 2112, 3336, 3392, 3613, 3698
ApoI RAATTY 7 cut(s) 370, 415, 722, 1017, 1939, 2602, 2950
AseI ATTAAT 1 cut(s) 4274
Asp700I GAANNNNTTC 4 cut(s) 544, 1278, 2025, 4290
AspA2I CCTAGG 1 cut(s) 1133
AspLEI GCGC 1 cut(s) 3658
AspS9I GGNCC 6 cut(s) 684, 728, 973, 1969, 2137, 3565
AsuC2I CCSGG 4 cut(s) 760, 761, 3031, 3706
AvaI CYCGRG 3 cut(s) 311, 603, 759
AvaII GGWCC 5 cut(s) 684, 728, 973, 1969, 3565
AvrII CCTAGG 1 cut(s) 1133
BaeGI GKGCMC 8 cut(s) 325, 773, 883, 2116, 3340, 3396, 3617, 3702
BalI TGGCCA 2 cut(s) 1620, 1985
BamHI GGATCC 2 cut(s) 2383, 3862
BanI GGYRCC 2 cut(s) 322, 1186
BbrPI CACGTG 1 cut(s) 751
BbsI GAAGAC 2 cut(s) 39, 4402
Bbv12I GWGCWC 7 cut(s) 128, 2116, 2146, 3340, 3396, 3617, 3702
BceAI ACGGC 3 cut(s) 593, 2124, 3204
BcgI CGANNNNNNTGC 4 cut(s) 2945, 2979, 3327, 3361
BciT130I CCWGG 3 cut(s) 1219, 1585, 3099
BciVI GTATCC 2 cut(s) 1005, 1381
BcnI CCSGG 4 cut(s) 760, 761, 3031, 3706
BcoDI GTCTC 8 cut(s) 164, 1082, 1563, 2183, 2876, 3360, 4056, 4070
BfaI CTAG 6 cut(s) 227, 246, 611, 1134, 2426, 2633
BfmI CTRYAG 5 cut(s) 646, 1695, 1809, 2151, 4119
BfuAI ACCTGC 2 cut(s) 2560, 4415
BfuI GTATCC 2 cut(s) 1005, 1381
BglII AGATCT 1 cut(s) 861
BlnI CCTAGG 1 cut(s) 1133
BlpI GCTNAGC 1 cut(s) 2097
BmcAI AGTACT 1 cut(s) 4117
Bme1390I CCNGG 7 cut(s) 760, 761, 1219, 1585, 3031, 3099, 3706
Bme18I GGWCC 5 cut(s) 684, 728, 973, 1969, 3565
BmeT110I CYCGRG 3 cut(s) 311, 603, 759
BmgT120I GGNCC 6 cut(s) 684, 728, 973, 1969, 2137, 3565
BmrFI CCNGG 7 cut(s) 760, 761, 1219, 1585, 3031, 3099, 3706
BmrI ACTGGG 5 cut(s) 934, 1574, 1751, 2702, 3938
BmsI GCATC 4 cut(s) 820, 1335, 2083, 2330
BmuI ACTGGG 5 cut(s) 934, 1574, 1751, 2702, 3938
BpiI GAAGAC 2 cut(s) 39, 4402
BpmI CTGGAG 3 cut(s) 1004, 1734, 2577
Bpu1102I GCTNAGC 1 cut(s) 2097
BpuEI CTTGAG 2 cut(s) 229, 2399
BpuMI CCSGG 4 cut(s) 760, 761, 3031, 3706
BsaAI YACGTR 1 cut(s) 751
BsaBI GATNNNNATC 3 cut(s) 891, 1230, 3540
BsaI GGTCTC 3 cut(s) 164, 1563, 2876
BsaWI WCCGGW 2 cut(s) 1472, 4352
Bse118I RCCGGY 2 cut(s) 1062, 3740
Bse3DI GCAATG 6 cut(s) 1439, 1986, 2001, 2139, 2299, 3089
Bse8I GATNNNNATC 3 cut(s) 891, 1230, 3540
BseBI CCWGG 3 cut(s) 1219, 1585, 3099
BseJI GATNNNNATC 3 cut(s) 891, 1230, 3540
BseMI GCAATG 6 cut(s) 1439, 1986, 2001, 2139, 2299, 3089
BseRI GAGGAG 2 cut(s) 488, 1933
BseSI GKGCMC 8 cut(s) 325, 773, 883, 2116, 3340, 3396, 3617, 3702
BseX3I CGGCCG 1 cut(s) 1064
BseYI CCCAGC 4 cut(s) 457, 2474, 2973, 3172
BsgI GTGCAG 3 cut(s) 1655, 2588, 2895
Bsh1285I CGRYCG 1 cut(s) 1067
BshFI GGCC 7 cut(s) 609, 1066, 1620, 1985, 2139, 3520, 3533
BshNI GGYRCC 2 cut(s) 322, 1186
BsiEI CGRYCG 1 cut(s) 1067
BsiHKAI GWGCWC 7 cut(s) 128, 2116, 2146, 3340, 3396, 3617, 3702
BsiHKCI CYCGRG 3 cut(s) 311, 603, 759
BsiSI CCGG 7 cut(s) 760, 1063, 1473, 3030, 3705, 3741, 4353
BslFI GGGAC 3 cut(s) 1982, 2270, 3171
BsmAI GTCTC 8 cut(s) 164, 1082, 1563, 2183, 2876, 3360, 4056, 4070
BsmBI CGTCTC 1 cut(s) 2183
BsmFI GGGAC 3 cut(s) 1982, 2270, 3171
BsmI GAATGC 7 cut(s) 1010, 1690, 2074, 2978, 3148, 3205, 3926
BsnI GGCC 7 cut(s) 609, 1066, 1620, 1985, 2139, 3520, 3533
Bso31I GGTCTC 3 cut(s) 164, 1563, 2876
BsoBI CYCGRG 3 cut(s) 311, 603, 759
Bsp1720I GCTNAGC 1 cut(s) 2097
Bsp19I CCATGG 1 cut(s) 3236
BspACI CCGC 7 cut(s) 198, 1067, 2273, 3131, 3684, 3692, 3866
BspANI GGCC 7 cut(s) 609, 1066, 1620, 1985, 2139, 3520, 3533
BspHI TCATGA 1 cut(s) 2043
BspMAI CTGCAG 2 cut(s) 1813, 4123
BspMI ACCTGC 2 cut(s) 2560, 4415
BspQI GCTCTTC 1 cut(s) 1319
BspT107I GGYRCC 2 cut(s) 322, 1186
BspTNI GGTCTC 3 cut(s) 164, 1563, 2876
BsrDI GCAATG 6 cut(s) 1439, 1986, 2001, 2139, 2299, 3089
BsrFI RCCGGY 2 cut(s) 1062, 3740
BssAI RCCGGY 2 cut(s) 1062, 3740
BssT1I CCWWGG 9 cut(s) 566, 1133, 1177, 1446, 3236, 3489, 3858, 3885, 4175
Bst2UI CCWGG 3 cut(s) 1219, 1585, 3099
Bst6I CTCTTC 6 cut(s) 230, 818, 1319, 2614, 3305, 3628
BstAPI GCANNNNNTGC 1 cut(s) 2003
BstBAI YACGTR 1 cut(s) 751
BstDSI CCRYGG 2 cut(s) 117, 3236
BstEII GGTNACC 1 cut(s) 3686
BstHHI GCGC 1 cut(s) 3658
BstMAI GTCTC 8 cut(s) 164, 1082, 1563, 2183, 2876, 3360, 4056, 4070
BstMCI CGRYCG 1 cut(s) 1067
BstNI CCWGG 3 cut(s) 1219, 1585, 3099
BstNSI RCATGY 8 cut(s) 2465, 2486, 3373, 3465, 3656, 4330, 4346, 4404
BstPI GGTNACC 1 cut(s) 3686
BstSCI CCNGG 7 cut(s) 758, 759, 1217, 1583, 3029, 3097, 3704
BstSFI CTRYAG 5 cut(s) 646, 1695, 1809, 2151, 4119
BstSLI GKGCMC 8 cut(s) 325, 773, 883, 2116, 3340, 3396, 3617, 3702
BstV2I GAAGAC 2 cut(s) 39, 4402
BstX2I RGATCY 9 cut(s) 189, 213, 861, 2383, 2790, 3601, 3769, 3862, 4232
BstXI CCANNNNNNTGG 4 cut(s) 118, 536, 567, 3221
BstYI RGATCY 9 cut(s) 189, 213, 861, 2383, 2790, 3601, 3769, 3862, 4232
BstZI CGGCCG 1 cut(s) 1064
BsuI GTATCC 2 cut(s) 1005, 1381
BsuRI GGCC 7 cut(s) 609, 1066, 1620, 1985, 2139, 3520, 3533
BtgI CCRYGG 2 cut(s) 117, 3236
BtgZI GCGATG 1 cut(s) 1157
BtsI GCAGTG 4 cut(s) 2571, 2735, 2883, 3879
BveI ACCTGC 2 cut(s) 2560, 4415
CciI TCATGA 1 cut(s) 2043
CfoI GCGC 1 cut(s) 3658
Cfr10I RCCGGY 2 cut(s) 1062, 3740
Cfr13I GGNCC 6 cut(s) 684, 728, 973, 1969, 2137, 3565
Cfr9I CCCGGG 1 cut(s) 759
CspCI CAANNNNNGTGG 2 cut(s) 2418, 2453
DrdI GACNNNNNNGTC 1 cut(s) 1892
DseDI GACNNNNNNGTC 1 cut(s) 1892
EaeI YGGCCR 3 cut(s) 1064, 1618, 1983
EagI CGGCCG 1 cut(s) 1064
Eam1104I CTCTTC 6 cut(s) 230, 818, 1319, 2614, 3305, 3628
EarI CTCTTC 6 cut(s) 230, 818, 1319, 2614, 3305, 3628
EclXI CGGCCG 1 cut(s) 1064
Eco130I CCWWGG 9 cut(s) 566, 1133, 1177, 1446, 3236, 3489, 3858, 3885, 4175
Eco147I AGGCCT 1 cut(s) 609
Eco31I GGTCTC 3 cut(s) 164, 1563, 2876
Eco32I GATATC 1 cut(s) 702
Eco47I GGWCC 5 cut(s) 684, 728, 973, 1969, 3565
Eco52I CGGCCG 1 cut(s) 1064
Eco57I CTGAAG 9 cut(s) 9, 51, 202, 238, 717, 2195, 2261, 3659, 3906
Eco72I CACGTG 1 cut(s) 751
Eco88I CYCGRG 3 cut(s) 311, 603, 759
Eco91I GGTNACC 1 cut(s) 3686
EcoO109I RGGNCCY 1 cut(s) 684
EcoO65I GGTNACC 1 cut(s) 3686
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 3 cut(s) 1217, 1583, 3097
EcoRV GATATC 1 cut(s) 702
EcoT14I CCWWGG 9 cut(s) 566, 1133, 1177, 1446, 3236, 3489, 3858, 3885, 4175
EcoT22I ATGCAT 1 cut(s) 1043
ErhI CCWWGG 9 cut(s) 566, 1133, 1177, 1446, 3236, 3489, 3858, 3885, 4175
Esp3I CGTCTC 1 cut(s) 2183
FalI AAGNNNNNCTT 4 cut(s) 2361, 2393, 2780, 2812
FaqI GGGAC 3 cut(s) 1982, 2270, 3171
FauI CCCGC 2 cut(s) 205, 3699
FauNDI CATATG 2 cut(s) 775, 1913
FspBI CTAG 6 cut(s) 227, 246, 611, 1134, 2426, 2633
GlaI GCGC 1 cut(s) 3657
GsaI CCCAGC 4 cut(s) 461, 2478, 2977, 3176
GsuI CTGGAG 3 cut(s) 1004, 1734, 2577
HaeIII GGCC 7 cut(s) 609, 1066, 1620, 1985, 2139, 3520, 3533
HapII CCGG 7 cut(s) 760, 1063, 1473, 3030, 3705, 3741, 4353
HhaI GCGC 1 cut(s) 3658
Hin6I GCGC 1 cut(s) 3656
HinP1I GCGC 1 cut(s) 3656
HincII GTYRAC 4 cut(s) 2365, 2545, 2630, 3645
HindII GTYRAC 4 cut(s) 2365, 2545, 2630, 3645
HindIII AAGCTT 6 cut(s) 517, 660, 2057, 2375, 3446, 4381
HpaI GTTAAC 1 cut(s) 2365
HpaII CCGG 7 cut(s) 760, 1063, 1473, 3030, 3705, 3741, 4353
Hpy188III TCNNGA 9 cut(s) 179, 336, 791, 1490, 1662, 1713, 2044, 2651, 2765
Hpy99I CGWCG 1 cut(s) 1776
HpyCH4IV ACGT 3 cut(s) 750, 2626, 2769
HpySE526I ACGT 3 cut(s) 750, 2626, 2769
HspAI GCGC 1 cut(s) 3656
KspAI GTTAAC 1 cut(s) 2365
LguI GCTCTTC 1 cut(s) 1319
LmnI GCTCC 7 cut(s) 123, 596, 793, 1129, 1361, 1538, 2558
LweI GCATC 4 cut(s) 820, 1335, 2083, 2330
MaeI CTAG 6 cut(s) 227, 246, 611, 1134, 2426, 2633
MaeII ACGT 3 cut(s) 750, 2626, 2769
MfeI CAATTG 1 cut(s) 1863
MflI RGATCY 9 cut(s) 189, 213, 861, 2383, 2790, 3601, 3769, 3862, 4232
MlsI TGGCCA 2 cut(s) 1620, 1985
MluNI TGGCCA 2 cut(s) 1620, 1985
MlyI GAGTC 6 cut(s) 748, 1141, 2645, 3317, 4072, 4416
MmeI TCCRAC 5 cut(s) 729, 1339, 2763, 3222, 4219
Mox20I TGGCCA 2 cut(s) 1620, 1985
Mph1103I ATGCAT 1 cut(s) 1043
MroXI GAANNNNTTC 4 cut(s) 544, 1278, 2025, 4290
MscI TGGCCA 2 cut(s) 1620, 1985
MslI CAYNNNNRTG 9 cut(s) 116, 816, 2091, 2907, 2990, 3219, 3546, 4139, 4391
Msp20I TGGCCA 2 cut(s) 1620, 1985
MspA1I CMGCKG 3 cut(s) 29, 200, 3133
MspI CCGG 7 cut(s) 760, 1063, 1473, 3030, 3705, 3741, 4353
MspR9I CCNGG 7 cut(s) 760, 761, 1219, 1585, 3031, 3099, 3706
MunI CAATTG 1 cut(s) 1863
Mva1269I GAATGC 7 cut(s) 1010, 1690, 2074, 2978, 3148, 3205, 3926
MvaI CCWGG 3 cut(s) 1219, 1585, 3099
NciI CCSGG 4 cut(s) 760, 761, 3031, 3706
NcoI CCATGG 1 cut(s) 3236
NdeI CATATG 2 cut(s) 775, 1913
NmeAIII GCCGAG 1 cut(s) 99
NmuCI GTSAC 6 cut(s) 751, 1248, 1894, 3589, 3934, 4427
NsiI ATGCAT 1 cut(s) 1043
NspI RCATGY 8 cut(s) 2465, 2486, 3373, 3465, 3656, 4330, 4346, 4404
OliI CACNNNNGTG 1 cut(s) 116
PaeI GCATGC 3 cut(s) 2465, 3656, 4404
PaeR7I CTCGAG 2 cut(s) 311, 603
PagI TCATGA 1 cut(s) 2043
PaqCI CACCTGC 1 cut(s) 4415
PceI AGGCCT 1 cut(s) 609
PciI ACATGT 3 cut(s) 3369, 3461, 4326
PciSI GCTCTTC 1 cut(s) 1319
PcsI WCGNNNNNNNCGW 1 cut(s) 1780
PctI GAATGC 7 cut(s) 1010, 1690, 2074, 2978, 3148, 3205, 3926
PdmI GAANNNNTTC 4 cut(s) 544, 1278, 2025, 4290
PflFI GACNNNGTC 1 cut(s) 331
PleI GAGTC 6 cut(s) 747, 1141, 2644, 3316, 4071, 4415
PmaCI CACGTG 1 cut(s) 751
PmlI CACGTG 1 cut(s) 751
PpsI GAGTC 6 cut(s) 747, 1141, 2644, 3316, 4071, 4415
Ppu21I YACGTR 1 cut(s) 751
PpuMI RGGWCCY 1 cut(s) 684
PscI ACATGT 3 cut(s) 3369, 3461, 4326
PshBI ATTAAT 1 cut(s) 4274
PsiI TTATAA 1 cut(s) 2034
Psp5II RGGWCCY 1 cut(s) 684
Psp6I CCWGG 3 cut(s) 1217, 1583, 3097
PspCI CACGTG 1 cut(s) 751
PspEI GGTNACC 1 cut(s) 3686
PspFI CCCAGC 4 cut(s) 457, 2474, 2973, 3172
PspGI CCWGG 3 cut(s) 1217, 1583, 3097
PspPI GGNCC 6 cut(s) 684, 728, 973, 1969, 2137, 3565
PspPPI RGGWCCY 1 cut(s) 684
PspXI VCTCGAGB 2 cut(s) 311, 603
PstI CTGCAG 2 cut(s) 1813, 4123
PsuI RGATCY 9 cut(s) 189, 213, 861, 2383, 2790, 3601, 3769, 3862, 4232
PsyI GACNNNGTC 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 29
RseI CAYNNNNRTG 9 cut(s) 116, 816, 2091, 2907, 2990, 3219, 3546, 4139, 4391
SapI GCTCTTC 1 cut(s) 1319
Sau96I GGNCC 6 cut(s) 684, 728, 973, 1969, 2137, 3565
ScaI AGTACT 1 cut(s) 4117
SchI GAGTC 6 cut(s) 748, 1141, 2645, 3317, 4072, 4416
ScrFI CCNGG 7 cut(s) 760, 761, 1219, 1585, 3031, 3099, 3706
SfaNI GCATC 4 cut(s) 820, 1335, 2083, 2330
SfcI CTRYAG 5 cut(s) 646, 1695, 1809, 2151, 4119
Sfr274I CTCGAG 2 cut(s) 311, 603
SinI GGWCC 5 cut(s) 684, 728, 973, 1969, 3565
SlaI CTCGAG 2 cut(s) 311, 603
SmaI CCCGGG 1 cut(s) 761
SmiMI CAYNNNNRTG 9 cut(s) 116, 816, 2091, 2907, 2990, 3219, 3546, 4139, 4391
SmlI CTYRAG 4 cut(s) 208, 311, 603, 2378
SmoI CTYRAG 4 cut(s) 208, 311, 603, 2378
SphI GCATGC 3 cut(s) 2465, 3656, 4404
SseBI AGGCCT 1 cut(s) 609
SsiI CCGC 7 cut(s) 198, 1067, 2273, 3131, 3684, 3692, 3866
SspI AATATT 4 cut(s) 1504, 2581, 2729, 3911
SspMI CTAG 6 cut(s) 227, 246, 611, 1134, 2426, 2633
StuI AGGCCT 1 cut(s) 609
StyD4I CCNGG 7 cut(s) 758, 759, 1217, 1583, 3029, 3097, 3704
StyI CCWWGG 9 cut(s) 566, 1133, 1177, 1446, 3236, 3489, 3858, 3885, 4175
TaiI ACGT 3 cut(s) 753, 2629, 2772
TatI WGTACW 5 cut(s) 65, 1905, 2620, 3261, 4115
TauI GCSGC 2 cut(s) 1069, 2276
TseFI GTSAC 6 cut(s) 751, 1248, 1894, 3589, 3934, 4427
Tsp45I GTSAC 6 cut(s) 751, 1248, 1894, 3589, 3934, 4427
TspGWI ACGGA 3 cut(s) 106, 1789, 3301
TspMI CCCGGG 1 cut(s) 759
Tth111I GACNNNGTC 1 cut(s) 331
VneI GTGCAC 5 cut(s) 2112, 3336, 3392, 3613, 3698
VpaK11BI GGWCC 5 cut(s) 684, 728, 973, 1969, 3565
VspI ATTAAT 1 cut(s) 4274
XapI RAATTY 7 cut(s) 370, 415, 722, 1017, 1939, 2602, 2950
XceI RCATGY 8 cut(s) 2465, 2486, 3373, 3465, 3656, 4330, 4346, 4404
XcmI CCANNNNNNNNNTGG 1 cut(s) 1486
XhoI CTCGAG 2 cut(s) 311, 603
XmaI CCCGGG 1 cut(s) 759
XmaJI CCTAGG 1 cut(s) 1133
XmnI GAANNNNTTC 4 cut(s) 544, 1278, 2025, 4290
XspI CTAG 6 cut(s) 227, 246, 611, 1134, 2426, 2633
ZrmI AGTACT 1 cut(s) 4117
Zsp2I ATGCAT 1 cut(s) 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.