RLG00000013221

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0020647
Physical Location & Seq
Forward (+)
27945376 .. 27946524
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013221

Sequence Viewer

Length: 1149 bp
ATGGCATCTGTCCAAGGAAATCTAGATTGGGAAGACTTGATGGTATCCTATGAGGCCGATTACCAAAGTGATGACGACTTGAAAAGTTTACACGCCAACTTCACCGCCGGTGCCAATGAAGTCATGAGGATGAGTAGCGGTCTTGATTGTTTCAAGAAAAGTATATTGTATCTGAGGAATTCCGACCAGGAGACTGTGGAGACAATCCTGTCCTGCAAAACACAAATACTTGCTGCAGAGGGCGATGTGGCGGCTGAGATTGAGAATTTTTTAGATAAATTCCTCATCGAGCCGTACCTCGAAAATAGTCACCAGACGCTTGCACTCTGCGAGGAGGCATTGCCAGACTTCATCGAAAAAGCTAATAAGGTCGCTTCATGCATTGAAGAAGTGGTGAGTACAAGGAGGATGTTGAAGAAGCTTAATACAGCTAAGATTAATTATGACGATTCCGCCAAAGAGATTGTTGAAAAGGTTGTATCTCTGCAGGACCAACATCTGACCTTTCTTGACAAACTGAACGCAGAATTCAGGAAGCTTAACAAGAGGCTGAAGAGTATTCGTACTGTTAGGAAGGTCGTACATGTGATATTCATAGGAATTCTTTCTGCTGTGTTTGCTTGCAGCGTCATGGCAGCATCTATGACTATTCCCGGTGTTGCAGCGGCCATGAGCTCTCATCCAGGTGTTGCAGCATTTATTGCTGCTAGTGCTGTTGCTCAAGCTTCTTCCTCGGCAATAAAAGTGGGAGACCATTGGCTTCTCACTATGATTCGAGAATCTGAATCAACTTTGGAGGCACGTCAGAATCTGATTGCGTCCATGAAATCCGGAACAAAGGATGCCATTAAAGTGTTGGACGACATTATGCATCATGTTGATAAGGTTATTGAAAGCGGGAATGATCCACGTTTGTCCAGTTTAAGCTTTGCAAATGAGCGACCAGTGGATTTGGACGCAAAGCTGAAGAAGTTTCAGAAAGAAATTAAGGAATTGGACAAGACAAGGGAGGCATGCATCCCTGTTATAGAAAATGCAAGGATACTGGTTGTCGATGAATATGAGAAGTTGAAGGAGATGTTGAAGGAGATGTTATCGGCCCGTCGCAAAAGCAAGCTCTTGTCAAAAAAATTGATTGTTTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

383

Amino Acids

42.62

Weight (kDa)

5.99

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 38 - 352 3e-22 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020647)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013221
rosa_multiflora Rmu_sc0000258.1_g000037
rosa_roxburghii Rroxscaffold_7G00190000 Rroxscaffold_7G00190030
rosa_samantha Rh6BG231100
rosa_wichuraiana Rw6G019840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 110
AccIII TCCGGA 1 cut(s) 830
AciI CCGC 6 cut(s) 105, 138, 251, 453, 665, 897
AclWI GGATC 1 cut(s) 899
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 5 cut(s) 178, 265, 278, 527, 600
AcuI CTGAAG 2 cut(s) 572, 986
AfaI GTAC 4 cut(s) 296, 400, 565, 582
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 8 cut(s) 82, 154, 386, 415, 470, 893, 1072, 1084
AjiI CACGTC 1 cut(s) 803
AjnI CCWGG 2 cut(s) 186, 682
AjuI GAANNNNNNNTTGG 2 cut(s) 10, 42
AluBI AGCT 9 cut(s) 362, 421, 431, 538, 675, 725, 927, 964, 1117
AluI AGCT 9 cut(s) 362, 421, 431, 538, 675, 725, 927, 964, 1117
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 3 cut(s) 185, 194, 744
AlwI GGATC 1 cut(s) 899
AlwNI CAGNNNCTG 1 cut(s) 811
Aor13HI TCCGGA 1 cut(s) 830
AoxI GGCC 3 cut(s) 54, 666, 1098
ApeKI GCWGC 6 cut(s) 233, 624, 635, 662, 692, 704
ApoI RAATTY 5 cut(s) 178, 265, 278, 527, 600
AseI ATTAAT 1 cut(s) 438
Asp700I GAANNNNTTC 1 cut(s) 604
AspS9I GGNCC 2 cut(s) 490, 1099
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 3 cut(s) 94, 302, 406
AvaII GGWCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 6 cut(s) 220, 636, 647, 674, 691, 704
BccI CCATC 1 cut(s) 34
BceAI ACGGC 1 cut(s) 277
BciT130I CCWGG 2 cut(s) 188, 684
BciVI GTATCC 2 cut(s) 55, 1035
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 3 cut(s) 185, 194, 744
BfaI CTAG 2 cut(s) 23, 708
BfmI CTRYAG 2 cut(s) 234, 485
BfuI GTATCC 2 cut(s) 55, 1035
BisI GCNGC 8 cut(s) 234, 252, 625, 636, 663, 666, 693, 705
BlsI GCNGC 8 cut(s) 235, 253, 626, 637, 664, 667, 694, 706
Bme1390I CCNGG 3 cut(s) 188, 654, 684
Bme18I GGWCC 1 cut(s) 490
BmgBI CACGTC 1 cut(s) 803
BmgT120I GGNCC 2 cut(s) 490, 1099
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 3 cut(s) 188, 654, 684
BmsI GCATC 5 cut(s) 14, 647, 832, 880, 1026
BpiI GAAGAC 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 654
BsaI GGTCTC 1 cut(s) 744
BsaJI CCNNGG 2 cut(s) 13, 732
BsaWI WCCGGW 1 cut(s) 830
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 3 cut(s) 918, 944, 1050
Bse3DI GCAATG 1 cut(s) 338
BseAI TCCGGA 1 cut(s) 830
BseBI CCWGG 2 cut(s) 188, 684
BseDI CCNNGG 2 cut(s) 13, 732
BseGI GGATG 5 cut(s) 135, 414, 679, 847, 1017
BseMI GCAATG 1 cut(s) 338
BseMII CTCAG 2 cut(s) 164, 246
BseNI ACTGG 3 cut(s) 918, 944, 1050
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 6 cut(s) 220, 636, 647, 674, 691, 704
BshFI GGCC 3 cut(s) 56, 668, 1100
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 677
BsiSI CCGG 3 cut(s) 108, 654, 831
BsmAI GTCTC 3 cut(s) 185, 194, 744
BsnI GGCC 3 cut(s) 56, 668, 1100
Bso31I GGTCTC 1 cut(s) 744
Bsp1286I GDGCHC 1 cut(s) 677
Bsp13I TCCGGA 1 cut(s) 830
Bsp143I GATC 1 cut(s) 904
BspACI CCGC 6 cut(s) 105, 138, 251, 453, 665, 897
BspANI GGCC 3 cut(s) 56, 668, 1100
BspCNI CTCAG 2 cut(s) 165, 247
BspEI TCCGGA 1 cut(s) 830
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 112
BspMAI CTGCAG 2 cut(s) 238, 489
BspPI GGATC 1 cut(s) 899
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 338
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 3 cut(s) 918, 944, 1050
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 13, 732
BssMI GATC 1 cut(s) 904
BssT1I CCWWGG 1 cut(s) 13
Bst2UI CCWGG 2 cut(s) 188, 684
Bst4CI ACNGT 2 cut(s) 196, 568
Bst6I CTCTTC 1 cut(s) 548
BstAPI GCANNNNNTGC 1 cut(s) 701
BstC8I GCNNGC 4 cut(s) 321, 622, 1015, 1115
BstDEI CTNAG 3 cut(s) 173, 255, 432
BstF5I GGATG 5 cut(s) 135, 414, 679, 847, 1017
BstKTI GATC 1 cut(s) 907
BstMAI GTCTC 3 cut(s) 185, 194, 744
BstMBI GATC 1 cut(s) 904
BstMWI GCNNNNNNNGC 3 cut(s) 617, 701, 710
BstNI CCWGG 2 cut(s) 188, 684
BstNSI RCATGY 2 cut(s) 587, 1017
BstSCI CCNGG 3 cut(s) 186, 652, 682
BstSFI CTRYAG 2 cut(s) 234, 485
BstV1I GCAGC 6 cut(s) 220, 636, 647, 674, 691, 704
BstV2I GAAGAC 1 cut(s) 39
BsuI GTATCC 2 cut(s) 55, 1035
BsuRI GGCC 3 cut(s) 56, 668, 1100
BtgZI GCGATG 1 cut(s) 258
BtrI CACGTC 1 cut(s) 803
BtsCI GGATG 5 cut(s) 135, 414, 679, 847, 1017
BtsIMutI CAGTG 1 cut(s) 951
Cac8I GCNNGC 4 cut(s) 321, 622, 1015, 1115
CaiI CAGNNNCTG 1 cut(s) 811
CciI TCATGA 1 cut(s) 123
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 490, 1099
CseI GACGC 4 cut(s) 325, 616, 807, 965
Csp6I GTAC 4 cut(s) 295, 399, 564, 581
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 8 cut(s) 124, 378, 584, 631, 670, 823, 875, 1014
CviQI GTAC 4 cut(s) 295, 399, 564, 581
DdeI CTNAG 3 cut(s) 173, 255, 432
DpnI GATC 1 cut(s) 906
DpnII GATC 1 cut(s) 904
DrdI GACNNNNNNGTC 1 cut(s) 208
DseDI GACNNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
EciI GGCGGA 1 cut(s) 442
Ecl136II GAGCTC 1 cut(s) 675
Eco130I CCWWGG 1 cut(s) 13
Eco24I GRGCYC 1 cut(s) 677
Eco31I GGTCTC 1 cut(s) 744
Eco47I GGWCC 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 2 cut(s) 572, 986
EcoICRI GAGCTC 1 cut(s) 675
EcoRI GAATTC 3 cut(s) 178, 527, 600
EcoRII CCWGG 2 cut(s) 186, 682
EcoT14I CCWWGG 1 cut(s) 13
EcoT22I ATGCAT 3 cut(s) 383, 873, 1019
EcoT38I GRGCYC 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 13
FaeI CATG 8 cut(s) 127, 381, 587, 634, 673, 826, 878, 1017
FatI CATG 8 cut(s) 123, 377, 583, 630, 669, 822, 874, 1013
FauI CCCGC 1 cut(s) 890
Fnu4HI GCNGC 8 cut(s) 234, 252, 625, 636, 663, 666, 693, 705
FokI GGATG 5 cut(s) 142, 421, 666, 854, 1004
FriOI GRGCYC 1 cut(s) 677
Fsp4HI GCNGC 8 cut(s) 234, 252, 625, 636, 663, 666, 693, 705
FspBI CTAG 2 cut(s) 23, 708
GluI GCNGC 8 cut(s) 234, 252, 625, 636, 663, 666, 693, 705
HaeIII GGCC 3 cut(s) 56, 668, 1100
HapII CCGG 3 cut(s) 108, 654, 831
HgaI GACGC 4 cut(s) 325, 616, 807, 965
Hin1II CATG 8 cut(s) 127, 381, 587, 634, 673, 826, 878, 1017
HindIII AAGCTT 4 cut(s) 419, 536, 723, 925
HinfI GANTC 5 cut(s) 449, 772, 779, 785, 808
HpaII CCGG 3 cut(s) 108, 654, 831
HphI GGTGA 3 cut(s) 94, 302, 406
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 7 cut(s) 174, 184, 501, 784, 807, 813, 978
Hpy188III TCNNGA 8 cut(s) 23, 124, 143, 154, 509, 532, 776, 831
Hpy8I GTNNAC 1 cut(s) 89
Hpy99I CGWCG 1 cut(s) 1107
HpyAV CCTTC 3 cut(s) 568, 1066, 1078
HpyCH4III ACNGT 2 cut(s) 196, 568
HpyCH4IV ACGT 2 cut(s) 802, 910
HpyF10VI GCNNNNNNNGC 3 cut(s) 617, 701, 710
HpyF3I CTNAG 3 cut(s) 173, 255, 432
HpySE526I ACGT 2 cut(s) 802, 910
Hsp92II CATG 8 cut(s) 127, 381, 587, 634, 673, 826, 878, 1017
Kpn2I TCCGGA 1 cut(s) 830
Kzo9I GATC 1 cut(s) 904
Lsp1109I GCAGC 6 cut(s) 220, 636, 647, 674, 691, 704
LweI GCATC 5 cut(s) 14, 647, 832, 880, 1026
MaeI CTAG 2 cut(s) 23, 708
MaeII ACGT 2 cut(s) 802, 910
MaeIII GTNAC 1 cut(s) 308
MalI GATC 1 cut(s) 906
MboI GATC 1 cut(s) 904
MboII GAAGA 6 cut(s) 44, 398, 427, 565, 720, 979
MhlI GDGCHC 1 cut(s) 677
MluCI AATT 9 cut(s) 178, 265, 278, 439, 527, 600, 984, 992, 1130
MmeI TCCRAC 2 cut(s) 207, 837
Mph1103I ATGCAT 3 cut(s) 383, 873, 1019
MroI TCCGGA 1 cut(s) 830
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 6 cut(s) 423, 438, 540, 849, 923, 987
MslI CAYNNNNRTG 3 cut(s) 128, 684, 851
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 3 cut(s) 108, 654, 831
MspR9I CCNGG 3 cut(s) 188, 654, 684
MvaI CCWGG 2 cut(s) 188, 684
MwoI GCNNNNNNNGC 3 cut(s) 617, 701, 710
NciI CCSGG 1 cut(s) 654
NdeII GATC 1 cut(s) 904
NlaIII CATG 8 cut(s) 127, 381, 587, 634, 673, 826, 878, 1017
NlaIV GGNNCC 1 cut(s) 112
NmeAIII GCCGAG 1 cut(s) 713
NmuCI GTSAC 1 cut(s) 308
NsiI ATGCAT 3 cut(s) 383, 873, 1019
NspI RCATGY 2 cut(s) 587, 1017
PaeI GCATGC 1 cut(s) 1017
PagI TCATGA 1 cut(s) 123
PciI ACATGT 1 cut(s) 583
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 5 cut(s) 449, 772, 779, 785, 808
PkrI GCNGC 8 cut(s) 235, 253, 626, 637, 664, 667, 694, 706
PscI ACATGT 1 cut(s) 583
PshBI ATTAAT 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 677
Psp6I CCWGG 2 cut(s) 186, 682
PspGI CCWGG 2 cut(s) 186, 682
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 2 cut(s) 490, 1099
PstI CTGCAG 2 cut(s) 238, 489
PstNI CAGNNNCTG 1 cut(s) 811
RsaI GTAC 4 cut(s) 296, 400, 565, 582
RsaNI GTAC 4 cut(s) 295, 399, 564, 581
RseI CAYNNNNRTG 3 cut(s) 128, 684, 851
SacI GAGCTC 1 cut(s) 677
SaqAI TTAA 6 cut(s) 423, 438, 540, 849, 923, 987
SatI GCNGC 8 cut(s) 234, 252, 625, 636, 663, 666, 693, 705
Sau3AI GATC 1 cut(s) 904
Sau96I GGNCC 2 cut(s) 490, 1099
ScrFI CCNGG 3 cut(s) 188, 654, 684
SduI GDGCHC 1 cut(s) 677
SfaNI GCATC 5 cut(s) 14, 647, 832, 880, 1026
SfcI CTRYAG 2 cut(s) 234, 485
SgrAI CRCCGGYG 1 cut(s) 107
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 3 cut(s) 128, 684, 851
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
SphI GCATGC 1 cut(s) 1017
Sse9I AATT 9 cut(s) 178, 265, 278, 439, 527, 600, 984, 992, 1130
SsiI CCGC 6 cut(s) 105, 138, 251, 453, 665, 897
SspMI CTAG 2 cut(s) 23, 708
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 3 cut(s) 186, 652, 682
StyI CCWWGG 1 cut(s) 13
TaaI ACNGT 2 cut(s) 196, 568
TaiI ACGT 2 cut(s) 805, 913
TaqI TCGA 5 cut(s) 288, 300, 354, 775, 1053
TasI AATT 9 cut(s) 178, 265, 278, 439, 527, 600, 984, 992, 1130
TatI WGTACW 1 cut(s) 398
TauI GCSGC 2 cut(s) 254, 668
TfiI GAWTC 5 cut(s) 449, 772, 779, 785, 808
Tru1I TTAA 6 cut(s) 423, 438, 540, 849, 923, 987
Tru9I TTAA 6 cut(s) 423, 438, 540, 849, 923, 987
TscAI CASTG 1 cut(s) 951
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 6 cut(s) 233, 624, 635, 662, 692, 704
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 6 cut(s) 132, 340, 366, 583, 839, 1071
TspRI CASTG 1 cut(s) 951
VpaK11BI GGWCC 1 cut(s) 490
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 5 cut(s) 178, 265, 278, 527, 600
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 2 cut(s) 587, 1017
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 2 cut(s) 23, 708
Zsp2I ATGCAT 3 cut(s) 383, 873, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.