Rroxscaffold_7G00190030

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0020647
Physical Location & Seq
Forward (+)
30026349 .. 30027308
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190030.1

Sequence Viewer

Length: 960 bp
ATGGCGTCTGTCCGAGGAAATCTAGATTGGGACGACTTGATGGTATCCTATCCGGCCGATTACCCAAGTGATGACGACTTGAAAAGGTTAGACGCCAACTTCACCGCCGGTGCCAATGAAGTCATGAGGATGAGTAGCGGTCTTGATCGTTTCAAGCAATGTATATCGTATCTGAGGAATTCCGACCAGAAGACTGTGGAGACAATCATGTCCTGCAAAACAAAAATACTTGCTGCAGAGGGCTATGTGGCGACTGAGATTGAGAATATTTTAGATAAACTCCTCATCAAGCAGTACTTCGAAAATAGTAACCAGACGCTTGCACTCTGCGAGGTGGAATTGCCAGACTTCATCGAAAAAGCTGATAAGGTCGCTTCATGCATTAAAGATGTGGTGCGTGAAAGGAGGATGTTGAAGAAGCTCAATACAGCTAAGATTAAGTATCAAGAATCCGCCAAAGAGATTGTTAAAAAGGTTGTATCTCTGCAGGACCAACATCTAACCTTGCTTAACAAACTGAAAGCAGAATTCAGGAAGCTTGACAAGAGGCTGAAGCGTATTCGTACTGTTAGGAAGGTCGTACATGTGATATTCATAGGAATTCTTTCTGCTGTGTTTGCTTGCAGCGTCATGGCAGCAGCTATGACTATTCCCGGTGTTGCAGCGGCCATGAGCTCTCATCCAGGTTTTGCAGCATTTATTGCTGCTTGTGCTGTTGTTCAAACTTCTTCCTCGGCAATAAAAGTGGGACAACATTGGCTTCTCACTATGATTCGAGAATCTGAATCAACTTTGAGGGCACCTCAGAATTTGATTGCGTCCATGGAATCCGGAACAAAGGATGCCATTAAAGTGTTGGACGACATTATGCGTCATGTTGATAAGGTTATTGCAAGGGGGAATGATCCACGTTTGTCCAGTTCAGCTCTGCAAATGAGGGACCTCTGGAGGCAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

319

Amino Acids

35.7

Weight (kDa)

9.18

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 39 - 296 5e-20 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020647)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013221
rosa_multiflora Rmu_sc0000258.1_g000037
rosa_roxburghii Rroxscaffold_7G00190000 Rroxscaffold_7G00190030
rosa_samantha Rh6BG231100
rosa_wichuraiana Rw6G019840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 208
AccB1I GGYRCC 2 cut(s) 110, 799
AccIII TCCGGA 1 cut(s) 830
AciI CCGC 4 cut(s) 105, 138, 453, 665
AclWI GGATC 1 cut(s) 899
AcoI YGGCCR 2 cut(s) 54, 666
AcsI RAATTY 4 cut(s) 178, 527, 600, 808
AcuI CTGAAG 1 cut(s) 572
AcyI GRCGYC 2 cut(s) 5, 93
AfaI GTAC 3 cut(s) 296, 565, 582
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 4 cut(s) 82, 154, 415, 722
AjnI CCWGG 1 cut(s) 682
AjuI GAANNNNNNNTTGG 2 cut(s) 10, 42
AluBI AGCT 8 cut(s) 362, 421, 431, 538, 641, 675, 926, 957
AluI AGCT 8 cut(s) 362, 421, 431, 538, 641, 675, 926, 957
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 1 cut(s) 899
Aor13HI TCCGGA 1 cut(s) 830
AoxI GGCC 2 cut(s) 54, 666
ApeKI GCWGC 7 cut(s) 233, 624, 635, 638, 662, 692, 704
ApoI RAATTY 4 cut(s) 178, 527, 600, 808
Asp700I GAANNNNTTC 1 cut(s) 604
AspS9I GGNCC 2 cut(s) 490, 940
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 300
AvaII GGWCC 2 cut(s) 490, 940
BaeGI GKGCMC 1 cut(s) 802
BanI GGYRCC 2 cut(s) 110, 799
BanII GRGCYC 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
BccI CCATC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 55
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 2 cut(s) 234, 485
BfuI GTATCC 1 cut(s) 55
BisI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
BlsI GCNGC 8 cut(s) 235, 626, 637, 640, 664, 667, 694, 706
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 2 cut(s) 654, 684
Bme18I GGWCC 2 cut(s) 490, 940
BmgT120I GGNCC 2 cut(s) 490, 940
BmiI GGNNCC 3 cut(s) 112, 801, 941
BmrFI CCNGG 2 cut(s) 654, 684
BmsI GCATC 1 cut(s) 832
BpiI GAAGAC 1 cut(s) 197
BplI GAGNNNNNCTC 2 cut(s) 787, 819
Bpu14I TTCGAA 1 cut(s) 300
BpuMI CCSGG 1 cut(s) 654
BsaHI GRCGYC 2 cut(s) 5, 93
BsaJI CCNNGG 3 cut(s) 13, 732, 822
BsaWI WCCGGW 1 cut(s) 830
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 918
Bse3DI GCAATG 1 cut(s) 164
BseAI TCCGGA 1 cut(s) 830
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 13, 732, 822
BseGI GGATG 4 cut(s) 135, 414, 679, 847
BseMI GCAATG 1 cut(s) 164
BseMII CTCAG 3 cut(s) 164, 246, 818
BseNI ACTGG 1 cut(s) 918
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 1 cut(s) 802
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 56, 668
BshNI GGYRCC 2 cut(s) 110, 799
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 677
BsiSI CCGG 4 cut(s) 53, 108, 654, 831
BslFI GGGAC 3 cut(s) 44, 762, 953
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 3 cut(s) 44, 762, 953
BsnI GGCC 2 cut(s) 56, 668
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 677, 802
Bsp13I TCCGGA 1 cut(s) 830
Bsp143I GATC 2 cut(s) 145, 904
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 4 cut(s) 105, 138, 453, 665
BspANI GGCC 2 cut(s) 56, 668
BspCNI CTCAG 3 cut(s) 165, 247, 817
BspEI TCCGGA 1 cut(s) 830
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 112, 801, 941
BspMAI CTGCAG 2 cut(s) 238, 489
BspPI GGATC 1 cut(s) 899
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 2 cut(s) 110, 799
BsrDI GCAATG 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 1 cut(s) 918
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 3 cut(s) 13, 732, 822
BssMI GATC 2 cut(s) 145, 904
BssNI GRCGYC 2 cut(s) 5, 93
BssT1I CCWWGG 1 cut(s) 822
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 2 cut(s) 196, 568
BstACI GRCGYC 2 cut(s) 5, 93
BstAPI GCANNNNNTGC 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 321, 622
BstDEI CTNAG 4 cut(s) 173, 255, 432, 804
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 4 cut(s) 135, 414, 679, 847
BstKTI GATC 2 cut(s) 148, 907
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 2 cut(s) 145, 904
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 617, 701, 710
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 652, 682
BstSFI CTRYAG 2 cut(s) 234, 485
BstSLI GKGCMC 1 cut(s) 802
BstV1I GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
BstV2I GAAGAC 1 cut(s) 197
BstZI CGGCCG 1 cut(s) 54
BsuI GTATCC 1 cut(s) 55
BsuRI GGCC 2 cut(s) 56, 668
BtgI CCRYGG 1 cut(s) 822
BtsCI GGATG 4 cut(s) 135, 414, 679, 847
Cac8I GCNNGC 2 cut(s) 321, 622
CciI TCATGA 1 cut(s) 123
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 490, 940
CseI GACGC 5 cut(s) 101, 325, 616, 807, 860
Csp6I GTAC 3 cut(s) 295, 564, 581
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 8 cut(s) 124, 208, 378, 584, 631, 670, 823, 875
CviQI GTAC 3 cut(s) 295, 564, 581
DdeI CTNAG 4 cut(s) 173, 255, 432, 804
DpnI GATC 2 cut(s) 147, 906
DpnII GATC 2 cut(s) 145, 904
DrdI GACNNNNNNGTC 1 cut(s) 208
DseDI GACNNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 54, 666
EagI CGGCCG 1 cut(s) 54
EciI GGCGGA 1 cut(s) 442
Ecl136II GAGCTC 1 cut(s) 675
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 822
Eco24I GRGCYC 1 cut(s) 677
Eco47I GGWCC 2 cut(s) 490, 940
Eco52I CGGCCG 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 1 cut(s) 572
EcoICRI GAGCTC 1 cut(s) 675
EcoO109I RGGNCCY 1 cut(s) 940
EcoRI GAATTC 3 cut(s) 178, 527, 600
EcoRII CCWGG 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 822
EcoT22I ATGCAT 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 822
FaeI CATG 8 cut(s) 127, 211, 381, 587, 634, 673, 826, 878
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 3 cut(s) 44, 762, 953
FatI CATG 8 cut(s) 123, 207, 377, 583, 630, 669, 822, 874
Fnu4HI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
FokI GGATG 4 cut(s) 142, 421, 666, 854
FriOI GRGCYC 1 cut(s) 677
Fsp4HI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
FspBI CTAG 1 cut(s) 23
GluI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
HaeIII GGCC 2 cut(s) 56, 668
HapII CCGG 4 cut(s) 53, 108, 654, 831
HgaI GACGC 5 cut(s) 101, 325, 616, 807, 860
Hin1I GRCGYC 2 cut(s) 5, 93
Hin1II CATG 8 cut(s) 127, 211, 381, 587, 634, 673, 826, 878
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 5 cut(s) 449, 772, 779, 785, 827
HpaII CCGG 4 cut(s) 53, 108, 654, 831
HphI GGTGA 1 cut(s) 94
Hpy188I TCNGA 5 cut(s) 14, 174, 184, 784, 807
Hpy188III TCNNGA 8 cut(s) 23, 124, 143, 446, 532, 776, 831, 946
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 2 cut(s) 196, 568
HpyCH4IV ACGT 1 cut(s) 910
HpyF10VI GCNNNNNNNGC 3 cut(s) 617, 701, 710
HpyF3I CTNAG 4 cut(s) 173, 255, 432, 804
HpySE526I ACGT 1 cut(s) 910
Hsp92I GRCGYC 2 cut(s) 5, 93
Hsp92II CATG 8 cut(s) 127, 211, 381, 587, 634, 673, 826, 878
Kpn2I TCCGGA 1 cut(s) 830
Kzo9I GATC 2 cut(s) 145, 904
Lsp1109I GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
LweI GCATC 1 cut(s) 832
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 910
MaeIII GTNAC 1 cut(s) 308
MalI GATC 2 cut(s) 147, 906
MboI GATC 2 cut(s) 145, 904
MboII GAAGA 3 cut(s) 202, 427, 720
MhlI GDGCHC 2 cut(s) 677, 802
MluCI AATT 5 cut(s) 178, 338, 527, 600, 808
MmeI TCCRAC 2 cut(s) 207, 837
Mph1103I ATGCAT 1 cut(s) 383
MroI TCCGGA 1 cut(s) 830
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 5 cut(s) 384, 438, 468, 510, 849
MslI CAYNNNNRTG 2 cut(s) 128, 851
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 4 cut(s) 53, 108, 654, 831
MspR9I CCNGG 2 cut(s) 654, 684
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 3 cut(s) 617, 701, 710
NciI CCSGG 1 cut(s) 654
NcoI CCATGG 1 cut(s) 822
NdeII GATC 2 cut(s) 145, 904
NlaIII CATG 8 cut(s) 127, 211, 381, 587, 634, 673, 826, 878
NlaIV GGNNCC 3 cut(s) 112, 801, 941
NmeAIII GCCGAG 1 cut(s) 713
NsiI ATGCAT 1 cut(s) 383
NspI RCATGY 1 cut(s) 587
NspV TTCGAA 1 cut(s) 300
PagI TCATGA 1 cut(s) 123
PciI ACATGT 1 cut(s) 583
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 5 cut(s) 449, 772, 779, 785, 827
PkrI GCNGC 8 cut(s) 235, 626, 637, 640, 664, 667, 694, 706
PpuMI RGGWCCY 1 cut(s) 940
PscI ACATGT 1 cut(s) 583
Psp124BI GAGCTC 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 940
Psp6I CCWGG 1 cut(s) 682
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 3 cut(s) 112, 801, 941
PspPI GGNCC 2 cut(s) 490, 940
PspPPI RGGWCCY 1 cut(s) 940
PstI CTGCAG 2 cut(s) 238, 489
RsaI GTAC 3 cut(s) 296, 565, 582
RsaNI GTAC 3 cut(s) 295, 564, 581
RseI CAYNNNNRTG 2 cut(s) 128, 851
SacI GAGCTC 1 cut(s) 677
SaqAI TTAA 5 cut(s) 384, 438, 468, 510, 849
SatI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
Sau3AI GATC 2 cut(s) 145, 904
Sau96I GGNCC 2 cut(s) 490, 940
ScaI AGTACT 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 654, 684
SduI GDGCHC 2 cut(s) 677, 802
SfaNI GCATC 1 cut(s) 832
SfcI CTRYAG 2 cut(s) 234, 485
SfuI TTCGAA 1 cut(s) 300
SgrAI CRCCGGYG 1 cut(s) 107
SinI GGWCC 2 cut(s) 490, 940
SmiMI CAYNNNNRTG 2 cut(s) 128, 851
Sse9I AATT 5 cut(s) 178, 338, 527, 600, 808
SsiI CCGC 4 cut(s) 105, 138, 453, 665
SspI AATATT 1 cut(s) 268
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 2 cut(s) 652, 682
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 2 cut(s) 196, 568
TaiI ACGT 1 cut(s) 913
TaqI TCGA 3 cut(s) 300, 354, 775
TasI AATT 5 cut(s) 178, 338, 527, 600, 808
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 5 cut(s) 449, 772, 779, 785, 827
Tru1I TTAA 5 cut(s) 384, 438, 468, 510, 849
Tru9I TTAA 5 cut(s) 384, 438, 468, 510, 849
TseI GCWGC 7 cut(s) 233, 624, 635, 638, 662, 692, 704
TspDTI ATGAA 4 cut(s) 132, 340, 366, 583
VpaK11BI GGWCC 2 cut(s) 490, 940
XapI RAATTY 4 cut(s) 178, 527, 600, 808
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 1 cut(s) 23
ZrmI AGTACT 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.