Rroxscaffold_7G00190000

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0020647
Physical Location & Seq
Forward (+)
29955483 .. 29956604
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190000.1

Sequence Viewer

Length: 1122 bp
ATGGCATCTGTCCAAGGAAATCTAGATTGGGACGACTTGATGGTATCCTATCCGGCCGATTACCCAAGTGATGACGACTTGAAAAGGTTAGACGCCAACTTCACCGCCGGTGCCAATGAAGTCATGAGGATGAGTAGCGGTCTTGATCGTTTCAAGCAATGTATATCGTATCTGAGGAATTCCGACCAGAAGACTGTGGAGACAATCATGTCCTGCAAAACACAAATACTTGCTGCAGAGGGCGATGTGGCGACTGAGATTGAGAATTTTTTAGATAAACTCCTCATCAAGCAGTACTTCGAAAATAGTAACCAGACGCTTGCACTCTGCGAGGTGGAATTGCCAGACTTCATCGAAAAAGCTGATAAGGTCGCTTCATGCATTAAAGATGTGGTGCGTGAAAGGAGGATGTTGAAGAAGCTCAATACAGCTAAGATTAAGTATCAAGATTCCGCCAAAGAGATTGTTAAAAAGGTTGTATCTCTGCAGGACCAACATCTAACCTTGCTTGACAAACTGAAAGCAGAATTCAGGAAGCTTGACAAGAGGCTGAAGCGTATTCGTACTGTTAGGAAGGTCGTACATGTGATATTCATAGGAATTCTTTCTGCTGTGTTTGCTTGCAGCGTCATGGCAGCAGCTATGACTATTCCCGGTGTTGCAGCGGCCATGAGCTCTCATCCAGGTGTTGCAGCATTTATTGCTGCTAGTGCTGTTGCTCAAGCTTCTTCCTCGGCAATAAAAGTGGGACAACATTGGCTTCTCACTATGATTCGAGAATCTGAATCAACTTCGGAGGCACGTCAGAATTTGATTGCGTCCATGGAATCCGGAACAAAGGATGCAATTAAAGTGTTGGACGACATTATGCGTCATGTTGATAAGGTTATTGCAAGCGGGAATGATCCACATTTGTCCAGTTCAAGCTCTGCAAATGAGGGACTAGTGGATTTGGAGGCAAAGCTGAAGAAGTTTCAGAAAGAAATTGAGGAATTGGACAAGACAAGGAAGGCATGCATCCCTGTTATACAAAAAGCAAGGGAACAGGTTGACGTTGAATATAAGAAGTTGAAGGAGGTGTTATGGGCCCGTCGCGAAAGCAAGCTCCCGTCAAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

373

Amino Acids

41.64

Weight (kDa)

8.8

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 39 - 350 6.7e-21 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020647)

Species Orthologous Gene IDs
rosa_laevigata RLG00000013221
rosa_multiflora Rmu_sc0000258.1_g000037
rosa_roxburghii Rroxscaffold_7G00190000 Rroxscaffold_7G00190030
rosa_samantha Rh6BG231100
rosa_wichuraiana Rw6G019840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 110
AccII CGCG 1 cut(s) 1095
AccIII TCCGGA 1 cut(s) 830
AciI CCGC 5 cut(s) 105, 138, 453, 665, 897
AclWI GGATC 1 cut(s) 899
AcoI YGGCCR 2 cut(s) 54, 666
AcsI RAATTY 5 cut(s) 178, 265, 527, 600, 808
AcuI CTGAAG 2 cut(s) 572, 986
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 3 cut(s) 296, 565, 582
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 6 cut(s) 82, 154, 415, 924, 1058, 1072
AhlI ACTAGT 1 cut(s) 943
AjiI CACGTC 1 cut(s) 803
AjnI CCWGG 1 cut(s) 682
AjuI GAANNNNNNNTTGG 2 cut(s) 10, 42
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 1 cut(s) 899
Aor13HI TCCGGA 1 cut(s) 830
AoxI GGCC 3 cut(s) 54, 666, 1086
ApaI GGGCCC 1 cut(s) 1090
ApeKI GCWGC 7 cut(s) 233, 624, 635, 638, 662, 692, 704
ApoI RAATTY 5 cut(s) 178, 265, 527, 600, 808
Asp700I GAANNNNTTC 1 cut(s) 604
AspS9I GGNCC 3 cut(s) 490, 1086, 1087
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 300
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 1090
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 2 cut(s) 677, 1090
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
BccI CCATC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 55
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 1 cut(s) 194
BcuI ACTAGT 1 cut(s) 943
BfaI CTAG 3 cut(s) 23, 708, 944
BfmI CTRYAG 2 cut(s) 234, 485
BfuI GTATCC 1 cut(s) 55
BisI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
BlsI GCNGC 8 cut(s) 235, 626, 637, 640, 664, 667, 694, 706
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 2 cut(s) 654, 684
Bme18I GGWCC 1 cut(s) 490
BmgBI CACGTC 1 cut(s) 803
BmgT120I GGNCC 3 cut(s) 490, 1086, 1087
BmiI GGNNCC 2 cut(s) 112, 1088
BmrFI CCNGG 2 cut(s) 654, 684
BmsI GCATC 3 cut(s) 14, 832, 1026
BpiI GAAGAC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 654
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 3 cut(s) 13, 732, 822
BsaWI WCCGGW 1 cut(s) 830
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 918
Bse3DI GCAATG 1 cut(s) 164
BseAI TCCGGA 1 cut(s) 830
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 13, 732, 822
BseGI GGATG 5 cut(s) 135, 414, 679, 847, 1017
BseMI GCAATG 1 cut(s) 164
BseMII CTCAG 2 cut(s) 164, 246
BseNI ACTGG 1 cut(s) 918
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 1 cut(s) 1090
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
Bsh1236I CGCG 1 cut(s) 1095
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 3 cut(s) 56, 668, 1088
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 677
BsiSI CCGG 4 cut(s) 53, 108, 654, 831
BslFI GGGAC 3 cut(s) 44, 762, 954
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 3 cut(s) 44, 762, 954
BsnI GGCC 3 cut(s) 56, 668, 1088
Bsp119I TTCGAA 1 cut(s) 300
Bsp120I GGGCCC 1 cut(s) 1086
Bsp1286I GDGCHC 2 cut(s) 677, 1090
Bsp13I TCCGGA 1 cut(s) 830
Bsp143I GATC 2 cut(s) 145, 904
Bsp19I CCATGG 1 cut(s) 822
Bsp68I TCGCGA 1 cut(s) 1095
BspACI CCGC 5 cut(s) 105, 138, 453, 665, 897
BspANI GGCC 3 cut(s) 56, 668, 1088
BspCNI CTCAG 2 cut(s) 165, 247
BspEI TCCGGA 1 cut(s) 830
BspFNI CGCG 1 cut(s) 1095
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 112, 1088
BspMAI CTGCAG 2 cut(s) 238, 489
BspPI GGATC 1 cut(s) 899
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 1 cut(s) 918
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 3 cut(s) 13, 732, 822
BssMI GATC 2 cut(s) 145, 904
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 13, 822
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 2 cut(s) 196, 568
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 5 cut(s) 321, 622, 895, 1015, 1103
BstDEI CTNAG 3 cut(s) 173, 255, 432
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 5 cut(s) 135, 414, 679, 847, 1017
BstFNI CGCG 1 cut(s) 1095
BstKTI GATC 2 cut(s) 148, 907
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 2 cut(s) 145, 904
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 617, 701, 710
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 2 cut(s) 587, 1017
BstSCI CCNGG 2 cut(s) 652, 682
BstSFI CTRYAG 2 cut(s) 234, 485
BstSLI GKGCMC 1 cut(s) 1090
BstUI CGCG 1 cut(s) 1095
BstV1I GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
BstV2I GAAGAC 1 cut(s) 197
BstZI CGGCCG 1 cut(s) 54
BsuI GTATCC 1 cut(s) 55
BsuRI GGCC 3 cut(s) 56, 668, 1088
BtgI CCRYGG 1 cut(s) 822
BtgZI GCGATG 1 cut(s) 258
BtrI CACGTC 1 cut(s) 803
BtsCI GGATG 5 cut(s) 135, 414, 679, 847, 1017
BtuMI TCGCGA 1 cut(s) 1095
Cac8I GCNNGC 5 cut(s) 321, 622, 895, 1015, 1103
CciI TCATGA 1 cut(s) 123
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 3 cut(s) 490, 1086, 1087
CseI GACGC 5 cut(s) 101, 325, 616, 807, 860
Csp6I GTAC 3 cut(s) 295, 564, 581
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 9 cut(s) 124, 208, 378, 584, 631, 670, 823, 875, 1014
CviQI GTAC 3 cut(s) 295, 564, 581
DdeI CTNAG 3 cut(s) 173, 255, 432
DpnI GATC 2 cut(s) 147, 906
DpnII GATC 2 cut(s) 145, 904
DrdI GACNNNNNNGTC 1 cut(s) 208
DseDI GACNNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 54, 666
EagI CGGCCG 1 cut(s) 54
EciI GGCGGA 1 cut(s) 442
Ecl136II GAGCTC 1 cut(s) 675
EclXI CGGCCG 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 13, 822
Eco24I GRGCYC 2 cut(s) 677, 1090
Eco47I GGWCC 1 cut(s) 490
Eco52I CGGCCG 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 2 cut(s) 572, 986
EcoICRI GAGCTC 1 cut(s) 675
EcoRI GAATTC 3 cut(s) 178, 527, 600
EcoRII CCWGG 1 cut(s) 682
EcoT14I CCWWGG 2 cut(s) 13, 822
EcoT22I ATGCAT 2 cut(s) 383, 1019
EcoT38I GRGCYC 2 cut(s) 677, 1090
ErhI CCWWGG 2 cut(s) 13, 822
FaeI CATG 9 cut(s) 127, 211, 381, 587, 634, 673, 826, 878, 1017
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 3 cut(s) 44, 762, 954
FatI CATG 9 cut(s) 123, 207, 377, 583, 630, 669, 822, 874, 1013
FauI CCCGC 1 cut(s) 890
Fnu4HI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
FokI GGATG 5 cut(s) 142, 421, 666, 854, 1004
FriOI GRGCYC 2 cut(s) 677, 1090
Fsp4HI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
FspBI CTAG 3 cut(s) 23, 708, 944
GluI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
HaeIII GGCC 3 cut(s) 56, 668, 1088
HapII CCGG 4 cut(s) 53, 108, 654, 831
HgaI GACGC 5 cut(s) 101, 325, 616, 807, 860
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 9 cut(s) 127, 211, 381, 587, 634, 673, 826, 878, 1017
HincII GTYRAC 1 cut(s) 1051
HindII GTYRAC 1 cut(s) 1051
HindIII AAGCTT 2 cut(s) 536, 723
HinfI GANTC 5 cut(s) 449, 772, 779, 785, 827
HpaII CCGG 4 cut(s) 53, 108, 654, 831
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 1051
Hpy188I TCNGA 6 cut(s) 174, 184, 784, 796, 807, 978
Hpy188III TCNNGA 8 cut(s) 23, 124, 143, 446, 532, 776, 831, 1094
Hpy8I GTNNAC 1 cut(s) 1051
Hpy99I CGWCG 1 cut(s) 1095
HpyAV CCTTC 3 cut(s) 568, 1003, 1066
HpyCH4III ACNGT 2 cut(s) 196, 568
HpyCH4IV ACGT 2 cut(s) 802, 1053
HpyF10VI GCNNNNNNNGC 3 cut(s) 617, 701, 710
HpyF3I CTNAG 3 cut(s) 173, 255, 432
HpySE526I ACGT 2 cut(s) 802, 1053
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 9 cut(s) 127, 211, 381, 587, 634, 673, 826, 878, 1017
Kpn2I TCCGGA 1 cut(s) 830
Kzo9I GATC 2 cut(s) 145, 904
LmnI GCTCC 1 cut(s) 1110
Lsp1109I GCAGC 7 cut(s) 220, 636, 647, 650, 674, 691, 704
LweI GCATC 3 cut(s) 14, 832, 1026
MaeI CTAG 3 cut(s) 23, 708, 944
MaeII ACGT 2 cut(s) 802, 1053
MaeIII GTNAC 1 cut(s) 308
MalI GATC 2 cut(s) 147, 906
MboI GATC 2 cut(s) 145, 904
MboII GAAGA 4 cut(s) 202, 427, 720, 979
MhlI GDGCHC 2 cut(s) 677, 1090
MluCI AATT 9 cut(s) 178, 265, 338, 527, 600, 808, 846, 984, 992
MmeI TCCRAC 2 cut(s) 207, 837
Mph1103I ATGCAT 2 cut(s) 383, 1019
MroI TCCGGA 1 cut(s) 830
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 4 cut(s) 384, 438, 468, 849
MslI CAYNNNNRTG 2 cut(s) 128, 684
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 4 cut(s) 53, 108, 654, 831
MspR9I CCNGG 2 cut(s) 654, 684
MvaI CCWGG 1 cut(s) 684
MvnI CGCG 1 cut(s) 1095
MwoI GCNNNNNNNGC 3 cut(s) 617, 701, 710
NciI CCSGG 1 cut(s) 654
NcoI CCATGG 1 cut(s) 822
NdeII GATC 2 cut(s) 145, 904
NlaIII CATG 9 cut(s) 127, 211, 381, 587, 634, 673, 826, 878, 1017
NlaIV GGNNCC 2 cut(s) 112, 1088
NmeAIII GCCGAG 1 cut(s) 713
NruI TCGCGA 1 cut(s) 1095
NsiI ATGCAT 2 cut(s) 383, 1019
NspI RCATGY 2 cut(s) 587, 1017
NspV TTCGAA 1 cut(s) 300
PaeI GCATGC 1 cut(s) 1017
PagI TCATGA 1 cut(s) 123
PciI ACATGT 1 cut(s) 583
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 5 cut(s) 449, 772, 779, 785, 827
PkrI GCNGC 8 cut(s) 235, 626, 637, 640, 664, 667, 694, 706
PscI ACATGT 1 cut(s) 583
Psp124BI GAGCTC 1 cut(s) 677
Psp6I CCWGG 1 cut(s) 682
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 2 cut(s) 112, 1088
PspOMI GGGCCC 1 cut(s) 1086
PspPI GGNCC 3 cut(s) 490, 1086, 1087
PstI CTGCAG 2 cut(s) 238, 489
RruI TCGCGA 1 cut(s) 1095
RsaI GTAC 3 cut(s) 296, 565, 582
RsaNI GTAC 3 cut(s) 295, 564, 581
RseI CAYNNNNRTG 2 cut(s) 128, 684
SacI GAGCTC 1 cut(s) 677
SaqAI TTAA 4 cut(s) 384, 438, 468, 849
SatI GCNGC 8 cut(s) 234, 625, 636, 639, 663, 666, 693, 705
Sau3AI GATC 2 cut(s) 145, 904
Sau96I GGNCC 3 cut(s) 490, 1086, 1087
ScaI AGTACT 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 654, 684
SduI GDGCHC 2 cut(s) 677, 1090
SfaNI GCATC 3 cut(s) 14, 832, 1026
SfcI CTRYAG 2 cut(s) 234, 485
SfuI TTCGAA 1 cut(s) 300
SgrAI CRCCGGYG 1 cut(s) 107
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 2 cut(s) 128, 684
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
SpeI ACTAGT 1 cut(s) 943
SphI GCATGC 1 cut(s) 1017
Sse9I AATT 9 cut(s) 178, 265, 338, 527, 600, 808, 846, 984, 992
SsiI CCGC 5 cut(s) 105, 138, 453, 665, 897
SspMI CTAG 3 cut(s) 23, 708, 944
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 2 cut(s) 652, 682
StyI CCWWGG 2 cut(s) 13, 822
TaaI ACNGT 2 cut(s) 196, 568
TaiI ACGT 2 cut(s) 805, 1056
TaqI TCGA 3 cut(s) 300, 354, 775
TasI AATT 9 cut(s) 178, 265, 338, 527, 600, 808, 846, 984, 992
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 5 cut(s) 449, 772, 779, 785, 827
Tru1I TTAA 4 cut(s) 384, 438, 468, 849
Tru9I TTAA 4 cut(s) 384, 438, 468, 849
TseI GCWGC 7 cut(s) 233, 624, 635, 638, 662, 692, 704
TspDTI ATGAA 4 cut(s) 132, 340, 366, 583
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 5 cut(s) 178, 265, 527, 600, 808
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 2 cut(s) 587, 1017
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 3 cut(s) 23, 708, 944
ZrmI AGTACT 1 cut(s) 296
Zsp2I ATGCAT 2 cut(s) 383, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.