RLG00000021392

proline-rich domain in spliceosome associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015845
Physical Location & Seq
Reverse (-)
74802155 .. 74811836
9682 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021392

Sequence Viewer

Length: 3753 bp
ATGGCAGTGTTCCCATCTCTGGTGTTGTTGGGCTTTTTGGTCTTTTTGGCTTCAACCCAGTCTCAGCCTGCTAAAGAAGTAGGTTATCGTAGTCTTTCGAGGCCTCTGATTGGTCATGATGGGAAGGTTTATGCTTGCTCTGAGAAAGATTTGCTTGCATTCGAAAGTAATGGGACTATTGCTTGGACCCTGCATTTGAATTATACATGCAGTTTTGATATGCCTCCAATTCATGGTGGTAAAGAAAAGATATATCTGGTTGCAGAAAATAGGGTGCTTAAGGTCAGCTTGCTATACAATGGGACTTCTAAACCTTCTGCAGAAGTTTTACTTGGTACAGAACCAACTAAAGAAGGAGATGGTGCGATTGTTGGGCTGGCAGTTAGCACGGTGAGTCCATCTGTATATGTAAACATTAGACGTAGGGGTCTCTTTGCATACACGGCGCGTGGACAATTGCTCTGGAGTGCTGGACCTGTGATCGATCAATATGGATATCAACAGGGTTGTAGGAAGAATATTGCAGATTGTTACTTTAACTCTGTCCCTGTGATTGATCAATGTGAGGCTAGTATATATATATCTAATAATGGAGGAGAATTATACTCTTTATCAACTCGTAGTCCTCGTTTCAAATGGATCCAGGATTTAAGTTCTTATGACAAAGTTTTCTCTATAACTCCTGGAAACAATGGTCGATTGTATGTTACTGTTCCAAACAAGGCTCTTTTGTTGGCACTAGATGTTTCTTCAGGAAATGTGCTGTGGGAGGGGAGTATTGGGCCATTGAGTACAGCAGATCACGCCCCAATTGTGGATTCTAATGGTTGGATATCTATTGGTTCATTAGATGGGTTCCTATACTCATTTTCGCCAACTGGGATTCTTAAGAAATTCTCAAAAGCTGCTGCAATGGACTCAGTGATCCAAGTCACTCCATTTCTTGACTGCACTGGCTTTGCAATTTATACTTCTCAGACCGAAATGGATGGGAAGATCAGCCACGCAGTAGGTGAATATACTTTTGTGTCAGCAATGAAACCCAAAAGTGTACTTTTTACCTTGTATGTTCCAGCAACCGGGTCCATATACTGGTCTGGAAGATATCCTGGTGATCAATTCTCATCCTTAATTTCTCATAGTGATCTCCACCATTTTATACTAGACGAGAGGATTGTTCTTGCCTTTGTTGCTGCTTCAAAGACCGGAAACCCACTGGCATGCCGTTCCACACGTCAAAGGTTTATGTCTAGCTGCTCCCAAGTAACGCCAAAGATTGTCAACATCTACACAGGTAGTCAAAGGGCAATACTCTTGTTCCTGTTTTTTGAATCTGTAATCTTGGTAGTACTAGCTGGACTTGTGCGGTTTTGCTGTATATTTTGGAGTAAAAAGAAGCTTCAGGGTCAAAACCTTGGAACTTTTCTTGAAAAGCGACGTTCTCTTAGACTCAAAAAGAAGGCATTCGATAGGACAATTACAGAACTTGAGCAAGAAGCAGCAAATGAAGCAGTGGCCAATGACGAGGCGCTTGAGAAATTAGGCAGTCTGTTACAAGAAAGGGAAGTCATCGAGAGGAAGCTATCAACGACCTATAGTTTGGGAAGAGATGGGATCAGCTCAAGGTCAAAATCTCTTCTTCCTTTGTATGATGGGAAAACAAGAAGCTATTATTTTCAAGGAGCTAAGAAAGAGAGTGTTACTGTCTTCCAAACATTGAATGATACATCATCTTCCAAAGAAAACAGCAGTGAGGGGGAGAGTAGCTGGACTTCTTATGAAGACAAGGGGAAAGAACCCTTGGAACAAGAGAGTTCAAGTGAGGGTGGGTTCTTTACTAGGGATTACCGGAGAAGTCCATCGGAGCCAGCTTCGAGCTCAACAGGATTTGTGGATCATGAGGGTGAAGTTAAGTCTCCACATGAGATGAAGATTGGTGCTGCGGCGGCGGCGGCGACTGAGCCCGCCTTGTTTCCTGCGCCGCGCTACCAGAATCCTAAATCGGAATTCAGCTGCGGCGGCGCATTTCAACAAAGCCCTAATCTCACTCAACCCGAGATTCCAAGTGTGGTTTCGGTGTGGCTCGTTATAGGAAGAAGTTGGTTCTTGATCGAAATGGAATCTGAGGATTTTATTGCTCTCCCGGATTCTGGTGACTCTATTGAGGAGGTGACCGACGAACTTAATAATGAGAGTGACTCTAAAGAGGTTGATGCTCAACCTACAAACTCTGAGGATAAAGAAGATAAACCAAACAGTGAGGATCTAGGGCGGGAGACCATTGCTCAATGTGAAGAAGGCGAATGCATACCAGAAGCAAGCCTTGCGAATGAATTGGTTGATAACGATTTAGATATGGAAATCGAGGATATAAACAACCTCCCTGCTTTGACTAGTTCTGGTCCCAAGGAGGGGGATGTTGAGAATGTAAATATTGGTTTGCACTCGACATTGTATGAGAATGGTCACCTTGCAGTTCAAGATACAAGTCCCGTTAGAATCCACAATACGGATGTCACTCCCAAAACAGGTGTTAAGAGGGCTCGAACAACAGTTGACGAGCAGCAAGCTTCAGTGCGTGTAACATTTAATTCTTTAACAAGAGCTAGTAAACACAAGCTTGAAGAACTGTTACAGCAGTGGTCAGAGTGGCATGCCAAACATGTTTCTTCATCTCAATCTTTTTGGATGGACAACCAAACAGGGACAGCACAGAACAGGGAATCGATTCCTTTGGATAGCAACTATGCGCCTCTATATGATCGTGGTTATACCTTAGGCTTGACTTTGGCTGGTAGTTCAACTAATCAGGAGGGGGGCTTGGAGATTATAGATGATGCTAGCCGTTGTTTCAATTGTGGTTCTTACAATCATGCGTTGAAGGAATGCCCCAAGCCTCGTGACCATATTGCTGTCAGTAATGCCCGCAAACAGCGCAAGATAAAAAAAAATCAAACTCCTAATTCTCGCAACTCAACTCGATATTATCAGAATTCCCCAGCTGGAAAATACGATGGTCTAAGGCCTGGTGCTCTCCATGCTGACACAAGGAAGCTTTTGGGTCTTGGGGAACTTGATCCACCTCCTTGGCTTAACAGAATGCGAGAAATTGGATACCCACCAGGATATTTAGATGTAGATGATGAGGACCAGCCATCTGGAATAATTATACACGGTGGTGAGGAAACTAAGGTTGAACAGGAGGATGGGGAAATCATCGAAACAGACCATCCTGAACCACCTGAACCACAGAGGAAAATGACAGTCGAATTTCCAGGAATAAATGCGCCAATCCCAGAAAATGCTGATGAGAGACTCTGGGCACCCGGCCTTTCATTTTCAGATCCCTCAAGGAACCGCTCGCACAACAGGTCAAACCATTATTCTGAACCTGTCAGCAGAGGCCATTATCGTGAGCAGAGGTGGTCTCGAGATTATAGAGATGATGGGCCTCCTGGCGTTGACCCAGGTTATGGGCCATCTAGTTATCCTCCGAGATATGGGAGTCATGATTACAGTTACAACTCCCGCAGTTTGAGTAATGCTTCAGCGCCTAGAAGCCCTACCTTTGGAAGGTCCCAAACAGACAGAGGCAGAAGAAGCAGTGAGTTTTCTTTTCCATACTCACCCACCAGGGATTCCAACCCCAAATATGACTATTTGAATGACGAAAGCAAGAATGACTGTGACCCTGATAGTTCAAGTCATAGTTGGGATAGGGTAGATAGGCATCGCCATCACCGTAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PSP

Protein Analysis

1251

Amino Acids

138.84

Weight (kDa)

5.55

Isoelectric Point (pI)

51.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSP PF04046 1008 - 1045 1e-10 PSP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16020
fragaria_vesca FvH4_6g45490
malus_domestica MD09G1082500.v1.1
prunus_persica Prupe.3G241200_v2.0.a1 Prupe.3G241200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0163431
rosa_laevigata RLG00000021392
rosa_multiflora Rmu_sc0028601.1_g000001
rosa_roxburghii Rroxscaffold_2G00087340
rosa_rugosa Rorug02G0502600
rosa_samantha Rh2AG569600 Rh2BG581700 Rh2CG551500 Rh2DG591600
rosa_wichuraiana Rw2G047140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 3322
AccB7I CCANNNNNTGG 3 cut(s) 233, 1092, 3473
AccBSI CCGCTC 1 cut(s) 3360
AccII CGCG 2 cut(s) 448, 1984
AclWI GGATC 8 cut(s) 634, 647, 919, 1622, 1902, 2271, 3071, 3338
AcoI YGGCCR 1 cut(s) 1515
AcsI RAATTY 4 cut(s) 893, 2006, 2992, 3269
AcuI CTGAAG 4 cut(s) 735, 1385, 2556, 3531
AfaI GTAC 4 cut(s) 337, 793, 1053, 1350
AflII CTTAAG 2 cut(s) 278, 887
AflIII ACRYGT 2 cut(s) 1232, 2662
AhlI ACTAGT 1 cut(s) 2393
AjiI CACGTC 1 cut(s) 1235
AjnI CCWGG 9 cut(s) 642, 682, 1108, 3025, 3121, 3274, 3454, 3466, 3632
AjuI GAANNNNNNNTTGG 8 cut(s) 315, 347, 802, 834, 1784, 1816, 2056, 2088
AleI CACNNNNGTG 1 cut(s) 3177
Alw21I GWGCWC 2 cut(s) 1880, 3034
Alw26I GTCTC 6 cut(s) 66, 434, 1920, 2270, 3307, 3432
AlwI GGATC 8 cut(s) 634, 647, 919, 1622, 1902, 2271, 3071, 3338
Ama87I CYCGRG 2 cut(s) 2054, 3429
AoxI GGCC 8 cut(s) 101, 782, 1515, 3023, 3328, 3403, 3449, 3476
ApeKI GCWGC 8 cut(s) 905, 908, 1193, 1254, 1499, 1940, 2013, 2563
ApoI RAATTY 4 cut(s) 893, 2006, 2992, 3269
ArsI GACNNNNNNTTYG 4 cut(s) 178, 210, 1582, 1614
Asp700I GAANNNNTTC 2 cut(s) 1463, 2727
AspLEI GCGC 9 cut(s) 448, 1531, 1981, 1986, 2024, 2752, 2937, 3289, 3553
AspS9I GGNCC 9 cut(s) 186, 473, 782, 1083, 2402, 3148, 3449, 3476, 3576
AsuC2I CCSGG 3 cut(s) 1081, 2144, 3327
AsuII TTCGAA 1 cut(s) 162
AsuNHI GCTAGC 1 cut(s) 2840
AvaI CYCGRG 2 cut(s) 2054, 3429
AvaII GGWCC 6 cut(s) 186, 473, 1083, 2402, 3148, 3576
AxyI CCTNAGG 1 cut(s) 2776
BaeGI GKGCMC 1 cut(s) 3325
BalI TGGCCA 1 cut(s) 1517
BamHI GGATCC 1 cut(s) 639
BanI GGYRCC 1 cut(s) 3322
BanII GRGCYC 3 cut(s) 1880, 1965, 2545
BarI GAAGNNNNNNTAC 4 cut(s) 1756, 1788, 2616, 2648
BauI CACGAG 1 cut(s) 2898
BbsI GAAGAC 2 cut(s) 1699, 1788
Bbv12I GWGCWC 2 cut(s) 1880, 3034
BbvI GCAGC 8 cut(s) 892, 895, 1180, 1241, 1511, 1927, 2000, 2575
BceAI ACGGC 3 cut(s) 459, 1209, 2829
BciT130I CCWGG 9 cut(s) 644, 684, 1110, 3027, 3123, 3276, 3456, 3468, 3634
BciVI GTATCC 1 cut(s) 3107
BclI TGATCA 2 cut(s) 556, 1114
BcnI CCSGG 3 cut(s) 1081, 2144, 3327
BcoDI GTCTC 6 cut(s) 66, 434, 1920, 2270, 3307, 3432
BcuI ACTAGT 1 cut(s) 2393
BfmI CTRYAG 2 cut(s) 318, 1594
BfoI RGCGCY 2 cut(s) 1532, 3554
BfrI CTTAAG 2 cut(s) 278, 887
BfuI GTATCC 1 cut(s) 3107
BmcAI AGTACT 1 cut(s) 1350
Bme18I GGWCC 6 cut(s) 186, 473, 1083, 2402, 3148, 3576
BmeT110I CYCGRG 2 cut(s) 2054, 3429
BmgBI CACGTC 1 cut(s) 1235
BmgT120I GGNCC 9 cut(s) 186, 473, 782, 1083, 2402, 3148, 3449, 3476, 3576
BmiI GGNNCC 9 cut(s) 188, 641, 857, 1084, 1866, 2404, 3324, 3356, 3578
BmrI ACTGGG 2 cut(s) 52, 888
BmsI GCATC 3 cut(s) 2203, 2827, 3739
BmtI GCTAGC 1 cut(s) 2844
BmuI ACTGGG 2 cut(s) 52, 888
BpiI GAAGAC 2 cut(s) 1699, 1788
BplI GAGNNNNNCTC 4 cut(s) 2183, 2215, 3412, 3444
BpmI CTGGAG 1 cut(s) 484
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 4 cut(s) 1508, 1553, 1606, 3334
BpuMI CCSGG 3 cut(s) 1081, 2144, 3327
Bsa29I ATCGAT 2 cut(s) 483, 2726
BsaBI GATNNNNATC 1 cut(s) 3729
BsaI GGTCTC 3 cut(s) 434, 2270, 3432
BsaJI CCNNGG 6 cut(s) 1414, 1800, 2406, 3086, 3466, 3633
BsaWI WCCGGW 2 cut(s) 1205, 1848
Bse1I ACTGG 5 cut(s) 58, 883, 958, 1097, 1221
Bse21I CCTNAGG 1 cut(s) 2776
Bse3DI GCAATG 3 cut(s) 918, 1041, 2280
Bse8I GATNNNNATC 1 cut(s) 3729
BseBI CCWGG 9 cut(s) 644, 684, 1110, 3027, 3123, 3276, 3456, 3468, 3634
BseCI ATCGAT 2 cut(s) 483, 2726
BseDI CCNNGG 6 cut(s) 1414, 1800, 2406, 3086, 3466, 3633
BseGI GGATG 7 cut(s) 994, 1124, 2422, 2518, 2694, 3211, 3229
BseJI GATNNNNATC 1 cut(s) 3729
BseMI GCAATG 3 cut(s) 918, 1041, 2280
BseMII CTCAG 7 cut(s) 77, 132, 933, 989, 1950, 2115, 2223
BseNI ACTGG 5 cut(s) 58, 883, 958, 1097, 1221
BseRI GAGGAG 2 cut(s) 609, 2180
BseSI GKGCMC 1 cut(s) 3325
BseXI GCAGC 8 cut(s) 892, 895, 1180, 1241, 1511, 1927, 2000, 2575
BseYI CCCAGC 1 cut(s) 2998
BsgI GTGCAG 1 cut(s) 934
Bsh1236I CGCG 2 cut(s) 448, 1984
BshFI GGCC 8 cut(s) 103, 784, 1517, 3025, 3330, 3405, 3451, 3478
BshNI GGYRCC 1 cut(s) 3322
BshVI ATCGAT 2 cut(s) 483, 2726
BsiHKAI GWGCWC 2 cut(s) 1880, 3034
BsiHKCI CYCGRG 2 cut(s) 2054, 3429
BsiSI CCGG 5 cut(s) 1080, 1206, 1849, 2144, 3327
BslFI GGGAC 7 cut(s) 187, 316, 530, 2388, 2475, 2719, 3562
BsmAI GTCTC 6 cut(s) 66, 434, 1920, 2270, 3307, 3432
BsmFI GGGAC 7 cut(s) 187, 316, 530, 2388, 2475, 2719, 3562
BsmI GAATGC 5 cut(s) 158, 1463, 2309, 2891, 3105
BsnI GGCC 8 cut(s) 103, 784, 1517, 3025, 3330, 3405, 3451, 3478
Bso31I GGTCTC 3 cut(s) 434, 2270, 3432
BsoBI CYCGRG 2 cut(s) 2054, 3429
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 5 cut(s) 1880, 1965, 2545, 3034, 3325
BspANI GGCC 8 cut(s) 103, 784, 1517, 3025, 3330, 3405, 3451, 3478
BspCNI CTCAG 7 cut(s) 76, 133, 932, 988, 1951, 2116, 2224
BspDI ATCGAT 2 cut(s) 483, 2726
BspFNI CGCG 2 cut(s) 448, 1984
BspHI TCATGA 3 cut(s) 115, 1897, 3508
BspLI GGNNCC 9 cut(s) 188, 641, 857, 1084, 1866, 2404, 3324, 3356, 3578
BspMAI CTGCAG 1 cut(s) 322
BspOI GCTAGC 1 cut(s) 2844
BspPI GGATC 8 cut(s) 634, 647, 919, 1622, 1902, 2271, 3071, 3338
BspT104I TTCGAA 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 3322
BspTI CTTAAG 2 cut(s) 278, 887
BspTNI GGTCTC 3 cut(s) 434, 2270, 3432
BsrBI CCGCTC 1 cut(s) 3360
BsrDI GCAATG 3 cut(s) 918, 1041, 2280
BsrI ACTGG 5 cut(s) 58, 883, 958, 1097, 1221
BssECI CCNNGG 6 cut(s) 1414, 1800, 2406, 3086, 3466, 3633
BssSI CACGAG 1 cut(s) 2898
BssT1I CCWWGG 4 cut(s) 1414, 1800, 2406, 3086
Bst2BI CACGAG 1 cut(s) 2898
Bst2UI CCWGG 9 cut(s) 644, 684, 1110, 3027, 3123, 3276, 3456, 3468, 3634
Bst6I CTCTTC 2 cut(s) 1600, 1641
BstAFI CTTAAG 2 cut(s) 278, 887
BstAPI GCANNNNNTGC 1 cut(s) 2324
BstBI TTCGAA 1 cut(s) 162
BstEII GGTNACC 2 cut(s) 2170, 2465
BstENI CCTNNNNNAGG 1 cut(s) 3571
BstF5I GGATG 7 cut(s) 994, 1124, 2422, 2518, 2694, 3211, 3229
BstFNI CGCG 2 cut(s) 448, 1984
BstH2I RGCGCY 2 cut(s) 1532, 3554
BstHHI GCGC 9 cut(s) 448, 1531, 1981, 1986, 2024, 2752, 2937, 3289, 3553
BstMAI GTCTC 6 cut(s) 66, 434, 1920, 2270, 3307, 3432
BstNI CCWGG 9 cut(s) 644, 684, 1110, 3027, 3123, 3276, 3456, 3468, 3634
BstNSI RCATGY 4 cut(s) 210, 1224, 2657, 2666
BstPI GGTNACC 2 cut(s) 2170, 2465
BstSFI CTRYAG 2 cut(s) 318, 1594
BstSLI GKGCMC 1 cut(s) 3325
BstUI CGCG 2 cut(s) 448, 1984
BstV1I GCAGC 8 cut(s) 892, 895, 1180, 1241, 1511, 1927, 2000, 2575
BstV2I GAAGAC 2 cut(s) 1699, 1788
BstX2I RGATCY 3 cut(s) 639, 2263, 3343
BstXI CCANNNNNNTGG 2 cut(s) 3087, 3158
BstYI RGATCY 3 cut(s) 639, 2263, 3343
Bsu15I ATCGAT 2 cut(s) 483, 2726
Bsu36I CCTNAGG 1 cut(s) 2776
BsuI GTATCC 1 cut(s) 3107
BsuRI GGCC 8 cut(s) 103, 784, 1517, 3025, 3330, 3405, 3451, 3478
BsuTUI ATCGAT 2 cut(s) 483, 2726
BtgZI GCGATG 1 cut(s) 3716
BtrI CACGTC 1 cut(s) 1235
BtsCI GGATG 7 cut(s) 994, 1124, 2422, 2518, 2694, 3211, 3229
BtsI GCAGTG 5 cut(s) 12, 1518, 1756, 2645, 3610
CciI TCATGA 3 cut(s) 115, 1897, 3508
CfoI GCGC 9 cut(s) 448, 1531, 1981, 1986, 2024, 2752, 2937, 3289, 3553
Cfr13I GGNCC 9 cut(s) 186, 473, 782, 1083, 2402, 3148, 3449, 3476, 3576
ClaI ATCGAT 2 cut(s) 483, 2726
Csp6I GTAC 4 cut(s) 336, 792, 1052, 1349
CviQI GTAC 4 cut(s) 336, 792, 1052, 1349
DrdI GACNNNNNNGTC 1 cut(s) 426
DseDI GACNNNNNNGTC 1 cut(s) 426
EaeI YGGCCR 1 cut(s) 1515
Eam1104I CTCTTC 2 cut(s) 1600, 1641
EarI CTCTTC 2 cut(s) 1600, 1641
Ecl136II GAGCTC 1 cut(s) 1878
Eco130I CCWWGG 4 cut(s) 1414, 1800, 2406, 3086
Eco147I AGGCCT 2 cut(s) 103, 3025
Eco24I GRGCYC 3 cut(s) 1880, 1965, 2545
Eco31I GGTCTC 3 cut(s) 434, 2270, 3432
Eco32I GATATC 3 cut(s) 497, 834, 1106
Eco47I GGWCC 6 cut(s) 186, 473, 1083, 2402, 3148, 3576
Eco53kI GAGCTC 1 cut(s) 1878
Eco57I CTGAAG 4 cut(s) 735, 1385, 2556, 3531
Eco81I CCTNAGG 1 cut(s) 2776
Eco88I CYCGRG 2 cut(s) 2054, 3429
Eco91I GGTNACC 2 cut(s) 2170, 2465
EcoICRI GAGCTC 1 cut(s) 1878
EcoNI CCTNNNNNAGG 1 cut(s) 3571
EcoO109I RGGNCCY 1 cut(s) 3576
EcoO65I GGTNACC 2 cut(s) 2170, 2465
EcoRI GAATTC 2 cut(s) 2006, 2992
EcoRII CCWGG 9 cut(s) 642, 682, 1108, 3025, 3121, 3274, 3454, 3466, 3632
EcoRV GATATC 3 cut(s) 497, 834, 1106
EcoT14I CCWWGG 4 cut(s) 1414, 1800, 2406, 3086
EcoT22I ATGCAT 1 cut(s) 2309
EcoT38I GRGCYC 3 cut(s) 1880, 1965, 2545
ErhI CCWWGG 4 cut(s) 1414, 1800, 2406, 3086
FalI AAGNNNNNCTT 8 cut(s) 138, 170, 272, 304, 315, 347, 2307, 2339
FaqI GGGAC 7 cut(s) 187, 316, 530, 2388, 2475, 2719, 3562
FauI CCCGC 4 cut(s) 1972, 2265, 2933, 3536
FbaI TGATCA 2 cut(s) 556, 1114
FokI GGATG 7 cut(s) 1001, 1111, 2429, 2525, 2701, 3216, 3218
FriOI GRGCYC 3 cut(s) 1880, 1965, 2545
GlaI GCGC 9 cut(s) 447, 1530, 1980, 1985, 2023, 2751, 2936, 3288, 3552
GsaI CCCAGC 1 cut(s) 3002
GsuI CTGGAG 1 cut(s) 484
HaeII RGCGCY 2 cut(s) 1532, 3554
HaeIII GGCC 8 cut(s) 103, 784, 1517, 3025, 3330, 3405, 3451, 3478
HapII CCGG 5 cut(s) 1080, 1206, 1849, 2144, 3327
HhaI GCGC 9 cut(s) 448, 1531, 1981, 1986, 2024, 2752, 2937, 3289, 3553
Hin6I GCGC 9 cut(s) 446, 1529, 1979, 1984, 2022, 2750, 2935, 3287, 3551
HinP1I GCGC 9 cut(s) 446, 1529, 1979, 1984, 2022, 2750, 2935, 3287, 3551
HincII GTYRAC 3 cut(s) 1282, 2557, 3463
HindII GTYRAC 3 cut(s) 1282, 2557, 3463
HindIII AAGCTT 4 cut(s) 1397, 2568, 2618, 3053
HpaII CCGG 5 cut(s) 1080, 1206, 1849, 2144, 3327
Hpy166II GTNNAC 7 cut(s) 412, 452, 1052, 1282, 2557, 2612, 3463
Hpy8I GTNNAC 7 cut(s) 412, 452, 1052, 1282, 2557, 2612, 3463
Hpy99I CGWCG 2 cut(s) 1440, 2180
HpyAV CCTTC 7 cut(s) 118, 324, 347, 1453, 2291, 2875, 3567
HpyCH4IV ACGT 3 cut(s) 421, 1234, 1438
HpySE526I ACGT 3 cut(s) 421, 1234, 1438
HspAI GCGC 9 cut(s) 446, 1529, 1979, 1984, 2022, 2750, 2935, 3287, 3551
Ksp22I TGATCA 2 cut(s) 556, 1114
LmnI GCTCC 3 cut(s) 1262, 1682, 1864
Lsp1109I GCAGC 8 cut(s) 892, 895, 1180, 1241, 1511, 1927, 2000, 2575
LweI GCATC 3 cut(s) 2203, 2827, 3739
MaeII ACGT 3 cut(s) 421, 1234, 1438
MbiI CCGCTC 1 cut(s) 3360
MfeI CAATTG 3 cut(s) 455, 810, 2854
MflI RGATCY 3 cut(s) 639, 2263, 3343
MhlI GDGCHC 5 cut(s) 1880, 1965, 2545, 3034, 3325
MlsI TGGCCA 1 cut(s) 1517
MluNI TGGCCA 1 cut(s) 1517
MlyI GAGTC 7 cut(s) 403, 911, 1443, 2150, 2192, 3309, 3514
MmeI TCCRAC 2 cut(s) 809, 3666
Mox20I TGGCCA 1 cut(s) 1517
Mph1103I ATGCAT 1 cut(s) 2309
MroXI GAANNNNTTC 2 cut(s) 1463, 2727
MscI TGGCCA 1 cut(s) 1517
MslI CAYNNNNRTG 4 cut(s) 1219, 1902, 3177, 3411
Msp20I TGGCCA 1 cut(s) 1517
MspA1I CMGCKG 2 cut(s) 2013, 3002
MspCI CTTAAG 2 cut(s) 278, 887
MspI CCGG 5 cut(s) 1080, 1206, 1849, 2144, 3327
MteI GCGCNGCGC 1 cut(s) 1982
MunI CAATTG 3 cut(s) 455, 810, 2854
Mva1269I GAATGC 5 cut(s) 158, 1463, 2309, 2891, 3105
MvaI CCWGG 9 cut(s) 644, 684, 1110, 3027, 3123, 3276, 3456, 3468, 3634
MvnI CGCG 2 cut(s) 448, 1984
NciI CCSGG 3 cut(s) 1081, 2144, 3327
NheI GCTAGC 1 cut(s) 2840
NlaIV GGNNCC 9 cut(s) 188, 641, 857, 1084, 1866, 2404, 3324, 3356, 3578
NmuCI GTSAC 8 cut(s) 931, 2153, 2170, 2195, 2465, 2515, 2900, 3686
NsiI ATGCAT 1 cut(s) 2309
NspI RCATGY 4 cut(s) 210, 1224, 2657, 2666
NspV TTCGAA 1 cut(s) 162
OliI CACNNNNGTG 1 cut(s) 3177
PaeI GCATGC 2 cut(s) 1224, 2657
PaeR7I CTCGAG 1 cut(s) 3429
PagI TCATGA 3 cut(s) 115, 1897, 3508
PceI AGGCCT 2 cut(s) 103, 3025
PciI ACATGT 1 cut(s) 2662
PcsI WCGNNNNNNNCGW 2 cut(s) 625, 3739
PctI GAATGC 5 cut(s) 158, 1463, 2309, 2891, 3105
PdmI GAANNNNTTC 2 cut(s) 1463, 2727
PflMI CCANNNNNTGG 3 cut(s) 233, 1092, 3473
PfoI TCCNGGA 4 cut(s) 642, 682, 2142, 3274
PleI GAGTC 7 cut(s) 402, 911, 1443, 2150, 2192, 3309, 3513
PpsI GAGTC 7 cut(s) 402, 911, 1443, 2150, 2192, 3309, 3513
PpuMI RGGWCCY 1 cut(s) 3576
PscI ACATGT 1 cut(s) 2662
Psp124BI GAGCTC 1 cut(s) 1880
Psp5II RGGWCCY 1 cut(s) 3576
Psp6I CCWGG 9 cut(s) 642, 682, 1108, 3025, 3121, 3274, 3454, 3466, 3632
PspEI GGTNACC 2 cut(s) 2170, 2465
PspFI CCCAGC 1 cut(s) 2998
PspGI CCWGG 9 cut(s) 642, 682, 1108, 3025, 3121, 3274, 3454, 3466, 3632
PspN4I GGNNCC 9 cut(s) 188, 641, 857, 1084, 1866, 2404, 3324, 3356, 3578
PspPI GGNCC 9 cut(s) 186, 473, 782, 1083, 2402, 3148, 3449, 3476, 3576
PspPPI RGGWCCY 1 cut(s) 3576
PstI CTGCAG 1 cut(s) 322
PsuI RGATCY 3 cut(s) 639, 2263, 3343
PvuII CAGCTG 2 cut(s) 2013, 3002
RsaI GTAC 4 cut(s) 337, 793, 1053, 1350
RsaNI GTAC 4 cut(s) 336, 792, 1052, 1349
RseI CAYNNNNRTG 4 cut(s) 1219, 1902, 3177, 3411
SacI GAGCTC 1 cut(s) 1880
Sau96I GGNCC 9 cut(s) 186, 473, 782, 1083, 2402, 3148, 3449, 3476, 3576
ScaI AGTACT 1 cut(s) 1350
SchI GAGTC 7 cut(s) 403, 911, 1443, 2150, 2192, 3309, 3514
SduI GDGCHC 5 cut(s) 1880, 1965, 2545, 3034, 3325
SfaNI GCATC 3 cut(s) 2203, 2827, 3739
SfcI CTRYAG 2 cut(s) 318, 1594
Sfr274I CTCGAG 1 cut(s) 3429
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 6 cut(s) 186, 473, 1083, 2402, 3148, 3576
SlaI CTCGAG 1 cut(s) 3429
SmiMI CAYNNNNRTG 4 cut(s) 1219, 1902, 3177, 3411
SmlI CTYRAG 7 cut(s) 278, 887, 1487, 1532, 1621, 3349, 3429
SmoI CTYRAG 7 cut(s) 278, 887, 1487, 1532, 1621, 3349, 3429
SpeI ACTAGT 1 cut(s) 2393
SphI GCATGC 2 cut(s) 1224, 2657
SseBI AGGCCT 2 cut(s) 103, 3025
SspI AATATT 2 cut(s) 520, 2434
SstI GAGCTC 1 cut(s) 1880
StuI AGGCCT 2 cut(s) 103, 3025
StyI CCWWGG 4 cut(s) 1414, 1800, 2406, 3086
TaiI ACGT 3 cut(s) 424, 1237, 1441
TaqII GACCGA 2 cut(s) 995, 2189
TatI WGTACW 3 cut(s) 791, 1051, 1348
TauI GCSGC 7 cut(s) 1946, 1949, 1952, 1955, 1984, 2019, 2022
TseFI GTSAC 8 cut(s) 931, 2153, 2170, 2195, 2465, 2515, 2900, 3686
TseI GCWGC 8 cut(s) 905, 908, 1193, 1254, 1499, 1940, 2013, 2563
Tsp45I GTSAC 8 cut(s) 931, 2153, 2170, 2195, 2465, 2515, 2900, 3686
TspDTI ATGAA 9 cut(s) 221, 834, 1052, 1521, 1794, 1943, 2346, 2661, 3324
TspGWI ACGGA 1 cut(s) 2525
Van91I CCANNNNNTGG 3 cut(s) 233, 1092, 3473
Vha464I CTTAAG 2 cut(s) 278, 887
VpaK11BI GGWCC 6 cut(s) 186, 473, 1083, 2402, 3148, 3576
XagI CCTNNNNNAGG 1 cut(s) 3571
XapI RAATTY 4 cut(s) 893, 2006, 2992, 3269
XceI RCATGY 4 cut(s) 210, 1224, 2657, 2666
XhoI CTCGAG 1 cut(s) 3429
XmnI GAANNNNTTC 2 cut(s) 1463, 2727
ZrmI AGTACT 1 cut(s) 1350
Zsp2I ATGCAT 1 cut(s) 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.