Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0015678

Physical Location & Seq

Genomic Coordinates Forward (+)

83001576 .. 83004454

Gene Structure 2879 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022383

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGGCTATTGCCCCTGTTTCGGTTTGAGGAAGGGCAAGAAGCTCAAGCGCGGCAAAGACGACGACGTTCACAAAACCACAATCAGCGACAACAAAAACCTTTCTTCCGATAATGCCACGCCTCAATTGTCTTCTGCTCAAGAAGATGGAACTAATGATCCTACTAAGGCACAGACATTCACCTTTCGTGAACTTGCAACTGCAACCAAAAATTTTAGGGCGGAATGCTTTATTGGGGAAGGTGGATTTGGGCCTGTATACAAAGGGAAATTGAGTTCTGATCGAGTTGTAGCTGTAAAGAAACTAGATCAGACAGGTCTTCAAGGAGACAAGGAGTTTCTGGTGGAAGTTCTAATGCTTTCTCTTCTGCGTCACCCAAACCTTGTAAGTTTAATTGGTTACTGTGCTGAAGGGGAGCAGCGCCTGCTTGTCTATGAATACATGCCCTTAGGGTCCTTGGCAGATCATCTCCATGATCTTAGACCTGATAAAGAACCACTTGATTGGAACACAAGGATGATGATAGCTGCTGGTGCAGCCAAAGGGTTGGAATATCTTCACCATGATGCTGAACCACCTGTCATATACAGGGATGTAAAATCAGCCAACATTTTGTTGGGGGAGGATTTTTTCCCTAAACTTTCTGATTTTGGGCTTGCAAAGTTTGGTCCTACTGGAGATAGATCGCATGTCTCCACCAGGGTCATGGGAACTCATGGTTACTGTGCTCCTGAGTATGCTAAAAGTGGAAAGTTGACAGCAAAGTCGGATATCTACAGTTTTGGGGTAGTCTTGCTGGAGTTGCTTACAGGACATAAGGCGGTTGATAGTACTCTTGGTCGTGAAAAACAGCTTGTTGAGTGGGCACGTCCTTTTATGAAAAATCCCAAGAATCTTACCATGCAATTTGCAGATCCATATCTTAGAGGGCAGTTTCCAGTACATATCTTGAGAAACGCATTAGAAGTGGCTTCCATGTGTCTAAGAGAGGATGCTTCTGCCCGACCGACCATGAGGGATGTGGTACTTGCCTTGAATTACTTGACAACCCATCCATATAATGAACCAAAGGTGTTGAGCATCAGCACTGAGCAGGTTGAAGGAATAAAGAGTTTCAATGCTTCAGGGAATAACTCTCCTAATGAAACGACATGCATGTTAAACAAGAACTTGGACAGAGAAAAAGCTGTTGCAGAGGCCAAGATGTGGGGAGAGACTTGGAGAGACAAACGAGGTGCACAAAATAGTCGTCATGATGCCTTTAGTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0031347 GO:0031349 GO:0036211 GO:0038023 GO:0040007 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1900457 GO:1900459 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

424

Amino Acids

47.1

Weight (kDa)

8.04

Isoelectric Point (pI)

30.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	77 - 342	8.5e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	77 - 343	1.7e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015678)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g53960 FvH4_6g53960 FvH4_6g53960 FvH4_6g53960
malus_domestica	MD09G1006900.v1.1
prunus_persica	Prupe.3G310200_v2.0.a1 Prupe.3G310200_v2.0.a1 Prupe.3G310200_v2.0.a1 Prupe.3G310200_v2.0.a1
pyrus_communis	pycom111g00510
rosa_chinensis	RchiOBHm_Chr2g0176121
rosa_laevigata	RLG00000022383
rosa_roxburghii	Rroxscaffold_2G00076750
rosa_rugosa	Rorug02G0590800
rosa_samantha	Rh2AG669500
rosa_wichuraiana	Rw2G054920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	763
Acc36I	ACCTGC	1 cut(s)	1084
AccB7I	CCANNNNNTGG	1 cut(s)	1206
AccI	GTMKAC	1 cut(s)	258
AccII	CGCG	1 cut(s)	51
AciI	CCGC	3 cut(s)	51, 221, 821
AclWI	GGATC	2 cut(s)	152, 908
AcsI	RAATTY	1 cut(s)	211
AcuI	CTGAAG	2 cut(s)	429, 1107
AfaI	GTAC	3 cut(s)	832, 942, 1026
AfiI	CCNNNNNNNGG	2 cut(s)	20, 1206
AgsI	TTSAA	4 cut(s)	323, 1036, 1100, 1117
AjiI	CACGTC	1 cut(s)	869
AjnI	CCWGG	1 cut(s)	698
AjuI	GAANNNNNNNTTGG	4 cut(s)	216, 231, 248, 263
AloI	GAACNNNNNNTCC	4 cut(s)	142, 174, 259, 291
AluBI	AGCT	5 cut(s)	43, 293, 527, 853, 1187
AluI	AGCT	5 cut(s)	43, 293, 527, 853, 1187
Alw21I	GWGCWC	2 cut(s)	730, 1240
Alw26I	GTCTC	4 cut(s)	321, 697, 1208, 1218
Alw44I	GTGCAC	1 cut(s)	1236
AlwI	GGATC	2 cut(s)	152, 908
AlwNI	CAGNNNCTG	1 cut(s)	424
AoxI	GGCC	2 cut(s)	251, 1197
ApaLI	GTGCAC	1 cut(s)	1236
ApeKI	GCWGC	3 cut(s)	418, 527, 536
ApoI	RAATTY	1 cut(s)	211
ArsI	GACNNNNNNTTYG	2 cut(s)	1234, 1266
Asp700I	GAANNNNTTC	1 cut(s)	555
AspLEI	GCGC	2 cut(s)	51, 423
AspS9I	GGNCC	3 cut(s)	251, 453, 668
AsuHPI	GGTGA	3 cut(s)	172, 365, 551
AvaII	GGWCC	2 cut(s)	453, 668
AxyI	CCTNAGG	1 cut(s)	448
BaeGI	GKGCMC	2 cut(s)	868, 1240
BbsI	GAAGAC	2 cut(s)	123, 311
Bbv12I	GWGCWC	2 cut(s)	730, 1240
BbvI	GCAGC	3 cut(s)	430, 514, 548
BccI	CCATC	2 cut(s)	140, 1059
BciT130I	CCWGG	1 cut(s)	700
BcoDI	GTCTC	4 cut(s)	321, 697, 1208, 1218
BfaI	CTAG	1 cut(s)	305
BfmI	CTRYAG	1 cut(s)	775
BfoI	RGCGCY	1 cut(s)	424
BfuAI	ACCTGC	1 cut(s)	1084
BisI	GCNGC	4 cut(s)	52, 419, 528, 537
BlsI	GCNGC	4 cut(s)	53, 420, 529, 538
BmcAI	AGTACT	1 cut(s)	832
Bme1390I	CCNGG	1 cut(s)	700
Bme18I	GGWCC	2 cut(s)	453, 668
BmgBI	CACGTC	1 cut(s)	869
BmgT120I	GGNCC	3 cut(s)	251, 453, 668
BmiI	GGNNCC	1 cut(s)	454
BmrFI	CCNGG	1 cut(s)	700
BmsI	GCATC	4 cut(s)	556, 982, 1089, 1246
BpiI	GAAGAC	2 cut(s)	123, 311
BpmI	CTGGAG	2 cut(s)	696, 818
BpuEI	CTTGAG	3 cut(s)	29, 123, 970
BsaBI	GATNNNNATC	1 cut(s)	918
BsaJI	CCNNGG	2 cut(s)	456, 699
Bsc4I	CCNNNNNNNGG	2 cut(s)	20, 1206
Bse1I	ACTGG	2 cut(s)	679, 938
Bse21I	CCTNAGG	1 cut(s)	448
Bse8I	GATNNNNATC	1 cut(s)	918
BseBI	CCWGG	1 cut(s)	700
BseDI	CCNNGG	2 cut(s)	456, 699
BseGI	GGATG	5 cut(s)	522, 598, 997, 1024, 1051
BseJI	GATNNNNATC	1 cut(s)	918
BseLI	CCNNNNNNNGG	2 cut(s)	20, 1206
BseMII	CTCAG	2 cut(s)	723, 1080
BseNI	ACTGG	2 cut(s)	679, 938
BseSI	GKGCMC	2 cut(s)	868, 1240
BseXI	GCAGC	3 cut(s)	430, 514, 548
BsgI	GTGCAG	1 cut(s)	555
Bsh1236I	CGCG	1 cut(s)	51
Bsh1285I	CGRYCG	1 cut(s)	1007
BshFI	GGCC	2 cut(s)	253, 1199
BsiEI	CGRYCG	1 cut(s)	1007
BsiHKAI	GWGCWC	2 cut(s)	730, 1240
BslI	CCNNNNNNNGG	2 cut(s)	20, 1206
BsmAI	GTCTC	4 cut(s)	321, 697, 1208, 1218
BsmI	GAATGC	1 cut(s)	230
BsnI	GGCC	2 cut(s)	253, 1199
Bsp1286I	GDGCHC	3 cut(s)	730, 868, 1240
Bsp143I	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
BspACI	CCGC	3 cut(s)	51, 221, 821
BspANI	GGCC	2 cut(s)	253, 1199
BspCNI	CTCAG	2 cut(s)	724, 1081
BspFNI	CGCG	1 cut(s)	51
BspHI	TCATGA	1 cut(s)	1252
BspLI	GGNNCC	1 cut(s)	454
BspMI	ACCTGC	1 cut(s)	1084
BspPI	GGATC	2 cut(s)	152, 908
BsrI	ACTGG	2 cut(s)	679, 938
BssECI	CCNNGG	2 cut(s)	456, 699
BssMI	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
BssNAI	GTATAC	1 cut(s)	259
BssT1I	CCWWGG	1 cut(s)	456
Bst1107I	GTATAC	1 cut(s)	259
Bst2UI	CCWGG	1 cut(s)	700
Bst4CI	ACNGT	3 cut(s)	404, 725, 779
Bst6I	CTCTTC	1 cut(s)	369
BstAPI	GCANNNNNTGC	1 cut(s)	424
BstC8I	GCNNGC	2 cut(s)	425, 657
BstDEI	CTNAG	7 cut(s)	165, 448, 479, 732, 923, 983, 1089
BstF5I	GGATG	5 cut(s)	522, 598, 997, 1024, 1051
BstFNI	CGCG	1 cut(s)	51
BstH2I	RGCGCY	1 cut(s)	424
BstHHI	GCGC	2 cut(s)	51, 423
BstKTI	GATC	7 cut(s)	160, 283, 310, 466, 478, 686, 916
BstMAI	GTCTC	4 cut(s)	321, 697, 1208, 1218
BstMBI	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
BstMCI	CGRYCG	1 cut(s)	1007
BstMWI	GCNNNNNNNGC	4 cut(s)	424, 533, 536, 802
BstNI	CCWGG	1 cut(s)	700
BstNSI	RCATGY	4 cut(s)	445, 692, 1155, 1159
BstSCI	CCNGG	1 cut(s)	698
BstSFI	CTRYAG	1 cut(s)	775
BstSLI	GKGCMC	2 cut(s)	868, 1240
BstUI	CGCG	1 cut(s)	51
BstV1I	GCAGC	3 cut(s)	430, 514, 548
BstV2I	GAAGAC	2 cut(s)	123, 311
BstX2I	RGATCY	1 cut(s)	913
BstXI	CCANNNNNNTGG	3 cut(s)	504, 547, 706
BstYI	RGATCY	1 cut(s)	913
BstZ17I	GTATAC	1 cut(s)	259
Bsu36I	CCTNAGG	1 cut(s)	448
BsuRI	GGCC	2 cut(s)	253, 1199
BtrI	CACGTC	1 cut(s)	869
BtsCI	GGATG	5 cut(s)	522, 598, 997, 1024, 1051
BtsIMutI	CAGTG	1 cut(s)	1086
BveI	ACCTGC	1 cut(s)	1084
Cac8I	GCNNGC	2 cut(s)	425, 657
CaiI	CAGNNNCTG	1 cut(s)	424
CciI	TCATGA	1 cut(s)	1252
CfoI	GCGC	2 cut(s)	51, 423
Cfr13I	GGNCC	3 cut(s)	251, 453, 668
CseI	GACGC	1 cut(s)	359
Csp6I	GTAC	3 cut(s)	831, 941, 1025
CviQI	GTAC	3 cut(s)	831, 941, 1025
DdeI	CTNAG	7 cut(s)	165, 448, 479, 732, 923, 983, 1089
DpnI	GATC	7 cut(s)	159, 282, 309, 465, 477, 685, 915
DpnII	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
DrdI	GACNNNNNNGTC	1 cut(s)	763
DseDI	GACNNNNNNGTC	1 cut(s)	763
Eam1104I	CTCTTC	1 cut(s)	369
EarI	CTCTTC	1 cut(s)	369
EciI	GGCGGA	1 cut(s)	236
Eco130I	CCWWGG	1 cut(s)	456
Eco32I	GATATC	1 cut(s)	772
Eco47I	GGWCC	2 cut(s)	453, 668
Eco57I	CTGAAG	2 cut(s)	429, 1107
Eco81I	CCTNAGG	1 cut(s)	448
EcoO109I	RGGNCCY	1 cut(s)	453
EcoRII	CCWGG	1 cut(s)	698
EcoRV	GATATC	1 cut(s)	772
EcoT14I	CCWWGG	1 cut(s)	456
EcoT22I	ATGCAT	1 cut(s)	1157
ErhI	CCWWGG	1 cut(s)	456
FalI	AAGNNNNNCTT	2 cut(s)	483, 515
FblI	GTMKAC	1 cut(s)	258
Fnu4HI	GCNGC	4 cut(s)	52, 419, 528, 537
FokI	GGATG	5 cut(s)	529, 605, 1004, 1031, 1038
Fsp4HI	GCNGC	4 cut(s)	52, 419, 528, 537
FspBI	CTAG	1 cut(s)	305
GlaI	GCGC	2 cut(s)	50, 422
GluI	GCNGC	4 cut(s)	52, 419, 528, 537
GsuI	CTGGAG	2 cut(s)	696, 818
HaeII	RGCGCY	1 cut(s)	424
HaeIII	GGCC	2 cut(s)	253, 1199
HgaI	GACGC	1 cut(s)	359
HhaI	GCGC	2 cut(s)	51, 423
Hin6I	GCGC	2 cut(s)	49, 421
HinP1I	GCGC	2 cut(s)	49, 421
HincII	GTYRAC	1 cut(s)	756
HindII	GTYRAC	1 cut(s)	756
HinfI	GANTC	1 cut(s)	892
HphI	GGTGA	3 cut(s)	172, 365, 551
Hpy166II	GTNNAC	5 cut(s)	70, 191, 259, 756, 1238
Hpy188I	TCNGA	5 cut(s)	109, 280, 312, 646, 769
Hpy188III	TCNNGA	6 cut(s)	140, 188, 731, 842, 949, 1253
Hpy8I	GTNNAC	5 cut(s)	70, 191, 259, 756, 1238
Hpy99I	CGWCG	2 cut(s)	65, 68
HpyAV	CCTTC	4 cut(s)	25, 233, 404, 1094
HpyCH4III	ACNGT	3 cut(s)	404, 725, 779
HpyCH4IV	ACGT	2 cut(s)	66, 868
HpyCH4V	TGCA	9 cut(s)	197, 203, 536, 659, 904, 911, 1155, 1193, 1238
HpyF10VI	GCNNNNNNNGC	4 cut(s)	424, 533, 536, 802
HpyF3I	CTNAG	7 cut(s)	165, 448, 479, 732, 923, 983, 1089
HpySE526I	ACGT	2 cut(s)	66, 868
HspAI	GCGC	2 cut(s)	49, 421
Kzo9I	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
LmnI	GCTCC	2 cut(s)	415, 733
Lsp1109I	GCAGC	3 cut(s)	430, 514, 548
LweI	GCATC	4 cut(s)	556, 982, 1089, 1246
MaeI	CTAG	1 cut(s)	305
MaeII	ACGT	2 cut(s)	66, 868
MaeIII	GTNAC	3 cut(s)	371, 398, 719
MalI	GATC	7 cut(s)	159, 282, 309, 465, 477, 685, 915
MboI	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
MboII	GAAGA	6 cut(s)	96, 123, 155, 311, 356, 548
MfeI	CAATTG	1 cut(s)	125
MflI	RGATCY	1 cut(s)	913
MhlI	GDGCHC	3 cut(s)	730, 868, 1240
MluCI	AATT	6 cut(s)	125, 211, 269, 393, 905, 1036
MmeI	TCCRAC	2 cut(s)	528, 747
MnlI	CCTC	8 cut(s)	21, 132, 616, 920, 982, 1008, 1189, 1226
Mph1103I	ATGCAT	1 cut(s)	1157
MroXI	GAANNNNTTC	1 cut(s)	555
MseI	TTAA	2 cut(s)	392, 1160
MslI	CAYNNNNRTG	3 cut(s)	471, 515, 564
MspR9I	CCNGG	1 cut(s)	700
MunI	CAATTG	1 cut(s)	125
Mva1269I	GAATGC	1 cut(s)	230
MvaI	CCWGG	1 cut(s)	700
MvnI	CGCG	1 cut(s)	51
MwoI	GCNNNNNNNGC	4 cut(s)	424, 533, 536, 802
NdeII	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
NlaIV	GGNNCC	1 cut(s)	454
NmuCI	GTSAC	1 cut(s)	371
NsiI	ATGCAT	1 cut(s)	1157
NspI	RCATGY	4 cut(s)	445, 692, 1155, 1159
PagI	TCATGA	1 cut(s)	1252
PctI	GAATGC	1 cut(s)	230
PdmI	GAANNNNTTC	1 cut(s)	555
PfeI	GAWTC	1 cut(s)	892
PflMI	CCANNNNNTGG	1 cut(s)	1206
PkrI	GCNGC	4 cut(s)	53, 420, 529, 538
PpuMI	RGGWCCY	1 cut(s)	453
Psp5II	RGGWCCY	1 cut(s)	453
Psp6I	CCWGG	1 cut(s)	698
PspGI	CCWGG	1 cut(s)	698
PspN4I	GGNNCC	1 cut(s)	454
PspPI	GGNCC	3 cut(s)	251, 453, 668
PspPPI	RGGWCCY	1 cut(s)	453
PstNI	CAGNNNCTG	1 cut(s)	424
PsuI	RGATCY	1 cut(s)	913
RsaI	GTAC	3 cut(s)	832, 942, 1026
RsaNI	GTAC	3 cut(s)	831, 941, 1025
RseI	CAYNNNNRTG	3 cut(s)	471, 515, 564
SaqAI	TTAA	2 cut(s)	392, 1160
SatI	GCNGC	4 cut(s)	52, 419, 528, 537
Sau3AI	GATC	7 cut(s)	157, 280, 307, 463, 475, 683, 913
Sau96I	GGNCC	3 cut(s)	251, 453, 668
ScaI	AGTACT	1 cut(s)	832
ScrFI	CCNGG	1 cut(s)	700
SduI	GDGCHC	3 cut(s)	730, 868, 1240
SfaNI	GCATC	4 cut(s)	556, 982, 1089, 1246
SfcI	CTRYAG	1 cut(s)	775
SinI	GGWCC	2 cut(s)	453, 668
SmiMI	CAYNNNNRTG	3 cut(s)	471, 515, 564
SmlI	CTYRAG	3 cut(s)	44, 138, 949
SmoI	CTYRAG	3 cut(s)	44, 138, 949
Sse9I	AATT	6 cut(s)	125, 211, 269, 393, 905, 1036
SsiI	CCGC	3 cut(s)	51, 221, 821
SspMI	CTAG	1 cut(s)	305
StyD4I	CCNGG	1 cut(s)	698
StyI	CCWWGG	1 cut(s)	456
TaaI	ACNGT	3 cut(s)	404, 725, 779
TaiI	ACGT	2 cut(s)	69, 871
TaqI	TCGA	1 cut(s)	283
TaqII	GACCGA	1 cut(s)	1021
TasI	AATT	6 cut(s)	125, 211, 269, 393, 905, 1036
TatI	WGTACW	2 cut(s)	830, 940
TauI	GCSGC	1 cut(s)	54
TfiI	GAWTC	1 cut(s)	892
Tru1I	TTAA	2 cut(s)	392, 1160
Tru9I	TTAA	2 cut(s)	392, 1160
TscAI	CASTG	1 cut(s)	1093
TseFI	GTSAC	1 cut(s)	371
TseI	GCWGC	3 cut(s)	418, 527, 536
Tsp45I	GTSAC	1 cut(s)	371
TspDTI	ATGAA	4 cut(s)	450, 893, 1077, 1158
TspRI	CASTG	1 cut(s)	1093
Van91I	CCANNNNNTGG	1 cut(s)	1206
VneI	GTGCAC	1 cut(s)	1236
VpaK11BI	GGWCC	2 cut(s)	453, 668
XapI	RAATTY	1 cut(s)	211
XceI	RCATGY	4 cut(s)	445, 692, 1155, 1159
XcmI	CCANNNNNNNNNTGG	3 cut(s)	613, 703, 1018
XmiI	GTMKAC	1 cut(s)	258
XmnI	GAANNNNTTC	1 cut(s)	555
XspI	CTAG	1 cut(s)	305
ZrmI	AGTACT	1 cut(s)	832
Zsp2I	ATGCAT	1 cut(s)	1157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.