RLG00000024134

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015413
Physical Location & Seq
Reverse (-)
32308432 .. 32310420
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024134

Sequence Viewer

Length: 1587 bp
ATGCAGAGCTACAAGTTGGTGGGTCTAATCATAGGGGTATTACTATCATGGGTTGCGTGGGTATCAGCGACAAAACTTCGACGGCATCGTTCCAAGGAGCTAAGACAGCTTCCTCCAGGACCAAGATGGTGGCCACTGGTTGGGAACATTTTTCAACTAGGCTGTGCACCGCCCCATGAGTCATTTGCCATATTAGCCCGCAAGCACGGCCCTATCATGACCCTGTGGCTAGGGTCAATGAGCACTGTGGTGATTTCATCGAGTATTTTGGCTCGTGAAATGTTTAAGAATCATGATGTGGTGCTAGCTGGTCGAAAAATATACGAGGCCATGAAAGGTGATTATGGTAATGAGGGTTCGCTCATCACGGCCCAATATGGCCCACACTGGCGCATGCTGAGGCGGCTATGCAACACTGAGTTCTTTGTCACGAGCCGCCTAAACGCCATGCGTGGTGTTCGTGGCACGTGCATCGATCGTATGGTGCAGTTTATAGAGGATGCTAGTACTAGTGACAAATCCGATATTGGTGCCAATGTTAAGAGCATTGATGTGGGTAGGTTCTTTTTCTTGATGGCTTTTAATCTAATTGGGAACCTTATGTTCTCCAAGGATCTTTTGGACCCAAAGTCGGAGAGAGGGGCTGGGTTCTTTTACCATGCAGGTAAGGTTATGGAGATGGCTGGGAAGCCTAATATAGCAGATTTCTTTCCTGTTCTGCGCTGGCTTGACCCGCAAGGCATTAGGAAGAATACCCAATTCCATGTCGAAAAAGCGTTTGAAATTGCTGGAGAGTTCATCAAGGAAAGACTGGAAAGTATGGCCAGTGATGGATATGATGTTGACATAAAGAAGACTAAGGACTTCTTAGATGTGCTCTTGCAGTACGACTCGCAGGGTGATGGTGTTGATGAAGAGGCCTATAGGTTCTCATCTCCGAGAACCATCAACGTGATTGTCTTTGAAATGTTCACGGCAGGGACTGATACCACTACAAGCACCTTGGAATGGGCGATGGCGGAGCTACTTAACAATCCAAAAACCCTAAAGAAAGTTCAGACCGAGCTAAGGAGCACCATAAGTTCTGGCAAGAAGCTTGAAGAGAGAGACATTGAAAACCTCCCCTACCTCAAAGCAGTAATCAAAGAGACTTTGAGGCTTCACCCACCTCTTCCTTTCTTAGTCCCACACATGGCCATGGCCTCTTGCGAAATGCTAGGACACCACATTCCAAAAGACACCCAAATCCTAGTCAATGTTTGGGCAATTGGACGTGACCCCAAAACTTGGGAAGACCCTTTAATATTCAAGCCAGAGAGGTTCTTGGAGCCTAACATGGTTGATTATAAAGGACACAACTTCGAGTTCATACCATTTGGTTCTGGGCGTCGGATGTGTCCTGCTGTGCCCCTAGTCTCAAGGGTGCTTCCTCTAGCCCTAGGATCGCTCTTACATTCCTTTGATTGGGTTTTACCAGATGGCCTCAAGCCGGAGAATATGGATATGGCTGAAAGGATGGGGATAACACTTCGCAAATCTGTCCCCTTAGAGGTGATACCTATACCTTACAAAGGAGATGCTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

529

Amino Acids

59.84

Weight (kDa)

8.73

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 509 3.4e-102 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52970 AT3G52970
fragaria_vesca FvH4_6g17540 FvH4_6g17540
malus_domestica MD14G1093800.v1.1
prunus_persica Prupe.7G120200_v2.0.a1
pyrus_communis pycom14g09130 pycom14g09150
rosa_chinensis RchiOBHm_Chr3g0471791
rosa_laevigata RLG00000024134
rosa_multiflora Rmu_sc0019096.1_g000001 Rmu_sc0019097.1_g000001
rosa_roxburghii Rroxscaffold_6G00409550
rosa_rugosa Rorug03G0119900
rosa_samantha Rh3DG178100
rosa_wichuraiana Rw3G016920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1347
Acc36I ACCTGC 1 cut(s) 653
AccB1I GGYRCC 1 cut(s) 530
AccB7I CCANNNNNTGG 2 cut(s) 140, 1287
AciI CCGC 6 cut(s) 170, 199, 403, 436, 734, 1019
AclWI GGATC 2 cut(s) 621, 1450
AcoI YGGCCR 3 cut(s) 131, 822, 1194
AcvI CACGTG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 1387
AfaI GTAC 2 cut(s) 508, 887
AgsI TTSAA 6 cut(s) 155, 782, 965, 1100, 1115, 1309
AhdI GACNNNNNGTC 1 cut(s) 628
AhlI ACTAGT 1 cut(s) 509
AjiI CACGTC 1 cut(s) 1274
AjnI CCWGG 1 cut(s) 115
AleI CACNNNNGTG 1 cut(s) 248
AluBI AGCT 7 cut(s) 9, 100, 109, 308, 1024, 1066, 1096
AluI AGCT 7 cut(s) 9, 100, 109, 308, 1024, 1066, 1096
Alw21I GWGCWC 4 cut(s) 169, 245, 879, 1076
Alw26I GTCTC 3 cut(s) 1101, 1142, 1420
Alw44I GTGCAC 1 cut(s) 165
AlwI GGATC 2 cut(s) 621, 1450
AlwNI CAGNNNCTG 1 cut(s) 983
ApaLI GTGCAC 1 cut(s) 165
AspA2I CCTAGG 1 cut(s) 1438
AspLEI GCGC 2 cut(s) 393, 723
AspS9I GGNCC 5 cut(s) 119, 209, 370, 380, 622
AsuHPI GGTGA 5 cut(s) 262, 350, 911, 1154, 1564
AsuNHI GCTAGC 1 cut(s) 304
AvaII GGWCC 2 cut(s) 119, 622
AvrII CCTAGG 1 cut(s) 1438
BaeGI GKGCMC 2 cut(s) 169, 1410
BalI TGGCCA 3 cut(s) 133, 824, 1196
BanI GGYRCC 1 cut(s) 530
BauI CACGAG 2 cut(s) 273, 430
BbrPI CACGTG 1 cut(s) 468
BbsI GAAGAC 2 cut(s) 860, 1299
Bbv12I GWGCWC 4 cut(s) 169, 245, 879, 1076
BbvCI CCTCAGC 1 cut(s) 398
BccI CCATC 9 cut(s) 120, 568, 673, 824, 896, 953, 1009, 1472, 1510
BceAI ACGGC 4 cut(s) 98, 223, 384, 990
BcgI CGANNNNNNTGC 4 cut(s) 454, 488, 512, 546
BciT130I CCWGG 1 cut(s) 117
BcoDI GTCTC 3 cut(s) 1101, 1142, 1420
BcuI ACTAGT 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 922
BfuAI ACCTGC 1 cut(s) 653
BisI GCNGC 2 cut(s) 404, 436
BlnI CCTAGG 1 cut(s) 1438
BlsI GCNGC 2 cut(s) 405, 437
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 2 cut(s) 119, 622
BmeRI GACNNNNNGTC 1 cut(s) 628
BmgBI CACGTC 1 cut(s) 1274
BmgT120I GGNCC 5 cut(s) 119, 209, 370, 380, 622
BmiI GGNNCC 4 cut(s) 532, 596, 624, 1329
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 4 cut(s) 94, 480, 490, 1567
BmtI GCTAGC 1 cut(s) 308
BpiI GAAGAC 2 cut(s) 860, 1299
BpmI CTGGAG 2 cut(s) 99, 810
Bpu10I CCTNAGC 2 cut(s) 398, 1067
BpuEI CTTGAG 2 cut(s) 1402, 1469
Bsa29I ATCGAT 1 cut(s) 474
BsaAI YACGTR 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 1387
BsaJI CCNNGG 5 cut(s) 93, 609, 1002, 1197, 1438
Bse1I ACTGG 4 cut(s) 141, 392, 816, 825
BseBI CCWGG 1 cut(s) 117
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 5 cut(s) 93, 609, 1002, 1197, 1438
BseGI GGATG 3 cut(s) 505, 1398, 1521
BseMII CTCAG 2 cut(s) 389, 408
BseNI ACTGG 4 cut(s) 141, 392, 816, 825
BseSI GKGCMC 2 cut(s) 169, 1410
BseYI CCCAGC 2 cut(s) 644, 683
BsgI GTGCAG 1 cut(s) 506
Bsh1285I CGRYCG 1 cut(s) 478
BshNI GGYRCC 1 cut(s) 530
BshVI ATCGAT 1 cut(s) 474
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 4 cut(s) 169, 245, 879, 1076
BsiSI CCGG 1 cut(s) 1490
BslFI GGGAC 3 cut(s) 994, 1169, 1526
BsmAI GTCTC 3 cut(s) 1101, 1142, 1420
BsmFI GGGAC 3 cut(s) 994, 1169, 1526
Bsp1286I GDGCHC 5 cut(s) 169, 245, 879, 1076, 1410
Bsp143I GATC 3 cut(s) 475, 613, 1442
Bsp19I CCATGG 1 cut(s) 1197
BspACI CCGC 6 cut(s) 170, 199, 403, 436, 734, 1019
BspCNI CTCAG 2 cut(s) 390, 409
BspDI ATCGAT 1 cut(s) 474
BspHI TCATGA 2 cut(s) 216, 292
BspLI GGNNCC 4 cut(s) 532, 596, 624, 1329
BspMI ACCTGC 1 cut(s) 653
BspOI GCTAGC 1 cut(s) 308
BspPI GGATC 2 cut(s) 621, 1450
BspT107I GGYRCC 1 cut(s) 530
BsrI ACTGG 4 cut(s) 141, 392, 816, 825
BssECI CCNNGG 5 cut(s) 93, 609, 1002, 1197, 1438
BssMI GATC 3 cut(s) 475, 613, 1442
BssNI GRCGYC 1 cut(s) 1387
BssSI CACGAG 2 cut(s) 273, 430
BssT1I CCWWGG 5 cut(s) 93, 609, 1002, 1197, 1438
Bst2BI CACGAG 2 cut(s) 273, 430
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 3 cut(s) 909, 1095, 1176
BstACI GRCGYC 1 cut(s) 1387
BstBAI YACGTR 1 cut(s) 468
BstC8I GCNNGC 5 cut(s) 199, 203, 306, 395, 725
BstDEI CTNAG 9 cut(s) 101, 398, 417, 858, 868, 1067, 1180, 1546, 1581
BstDSI CCRYGG 1 cut(s) 1197
BstENI CCTNNNNNAGG 1 cut(s) 1569
BstF5I GGATG 3 cut(s) 505, 1398, 1521
BstHHI GCGC 2 cut(s) 393, 723
BstKTI GATC 3 cut(s) 478, 616, 1445
BstMAI GTCTC 3 cut(s) 1101, 1142, 1420
BstMBI GATC 3 cut(s) 475, 613, 1442
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 5 cut(s) 106, 194, 207, 403, 733
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 922
BstSLI GKGCMC 2 cut(s) 169, 1410
BstV2I GAAGAC 2 cut(s) 860, 1299
BstX2I RGATCY 1 cut(s) 613
BstXI CCANNNNNNTGG 1 cut(s) 129
BstYI RGATCY 1 cut(s) 613
Bsu15I ATCGAT 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 1197
BtgZI GCGATG 1 cut(s) 1028
BtrI CACGTC 1 cut(s) 1274
BtsCI GGATG 3 cut(s) 505, 1398, 1521
BtsIMutI CAGTG 5 cut(s) 134, 243, 385, 414, 832
BveI ACCTGC 1 cut(s) 653
Cac8I GCNNGC 5 cut(s) 199, 203, 306, 395, 725
CaiI CAGNNNCTG 1 cut(s) 983
CciI TCATGA 2 cut(s) 216, 292
CfoI GCGC 2 cut(s) 393, 723
Cfr13I GGNCC 5 cut(s) 119, 209, 370, 380, 622
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 1 cut(s) 1376
Csp6I GTAC 2 cut(s) 507, 886
CviQI GTAC 2 cut(s) 507, 886
DdeI CTNAG 9 cut(s) 101, 398, 417, 858, 868, 1067, 1180, 1546, 1581
DpnI GATC 3 cut(s) 477, 615, 1444
DpnII GATC 3 cut(s) 475, 613, 1442
DriI GACNNNNNGTC 1 cut(s) 628
EaeI YGGCCR 3 cut(s) 131, 822, 1194
Eam1104I CTCTTC 3 cut(s) 909, 1095, 1176
Eam1105I GACNNNNNGTC 1 cut(s) 628
EarI CTCTTC 3 cut(s) 909, 1095, 1176
EciI GGCGGA 1 cut(s) 1034
Eco130I CCWWGG 5 cut(s) 93, 609, 1002, 1197, 1438
Eco147I AGGCCT 1 cut(s) 920
Eco47I GGWCC 2 cut(s) 119, 622
Eco72I CACGTG 1 cut(s) 468
EcoNI CCTNNNNNAGG 1 cut(s) 1569
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 5 cut(s) 93, 609, 1002, 1197, 1438
ErhI CCWWGG 5 cut(s) 93, 609, 1002, 1197, 1438
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 3 cut(s) 994, 1169, 1526
FauI CCCGC 2 cut(s) 206, 741
Fnu4HI GCNGC 2 cut(s) 404, 436
FokI GGATG 3 cut(s) 512, 1405, 1528
Fsp4HI GCNGC 2 cut(s) 404, 436
GlaI GCGC 2 cut(s) 392, 722
GluI GCNGC 2 cut(s) 404, 436
GsaI CCCAGC 2 cut(s) 648, 687
GsuI CTGGAG 2 cut(s) 99, 810
HapII CCGG 1 cut(s) 1490
HgaI GACGC 1 cut(s) 1376
HhaI GCGC 2 cut(s) 393, 723
Hin1I GRCGYC 1 cut(s) 1387
Hin6I GCGC 2 cut(s) 391, 721
HinP1I GCGC 2 cut(s) 391, 721
HincII GTYRAC 1 cut(s) 844
HindII GTYRAC 1 cut(s) 844
HindIII AAGCTT 1 cut(s) 1094
HinfI GANTC 3 cut(s) 179, 289, 890
HpaII CCGG 1 cut(s) 1490
HphI GGTGA 5 cut(s) 262, 350, 911, 1154, 1564
Hpy166II GTNNAC 3 cut(s) 167, 844, 972
Hpy188I TCNGA 5 cut(s) 523, 634, 939, 1059, 1392
Hpy188III TCNNGA 5 cut(s) 217, 275, 293, 430, 571
Hpy8I GTNNAC 3 cut(s) 167, 844, 972
Hpy99I CGWCG 2 cut(s) 84, 1392
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 3 cut(s) 467, 951, 1273
HpyCH4V TGCA 7 cut(s) 4, 167, 411, 471, 487, 662, 883
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 194, 207, 403, 733
HpyF3I CTNAG 9 cut(s) 101, 398, 417, 858, 868, 1067, 1180, 1546, 1581
HpySE526I ACGT 3 cut(s) 467, 951, 1273
Hsp92I GRCGYC 1 cut(s) 1387
HspAI GCGC 2 cut(s) 391, 721
Kzo9I GATC 3 cut(s) 475, 613, 1442
LmnI GCTCC 4 cut(s) 97, 1021, 1071, 1327
LweI GCATC 4 cut(s) 94, 480, 490, 1567
MaeII ACGT 3 cut(s) 467, 951, 1273
MaeIII GTNAC 3 cut(s) 427, 512, 1274
MalI GATC 3 cut(s) 477, 615, 1444
MboI GATC 3 cut(s) 475, 613, 1442
MboII GAAGA 6 cut(s) 760, 865, 926, 1112, 1163, 1304
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 5 cut(s) 169, 245, 879, 1076, 1410
MlsI TGGCCA 3 cut(s) 133, 824, 1196
MluCI AATT 4 cut(s) 588, 758, 783, 1266
MluNI TGGCCA 3 cut(s) 133, 824, 1196
MlyI GAGTC 2 cut(s) 188, 884
MmeI TCCRAC 2 cut(s) 612, 1370
Mox20I TGGCCA 3 cut(s) 133, 824, 1196
MscI TGGCCA 3 cut(s) 133, 824, 1196
MseI TTAA 5 cut(s) 285, 540, 582, 1029, 1301
MslI CAYNNNNRTG 4 cut(s) 248, 551, 950, 1196
Msp20I TGGCCA 3 cut(s) 133, 824, 1196
MspI CCGG 1 cut(s) 1490
MspR9I CCNGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 1266
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 5 cut(s) 106, 194, 207, 403, 733
NcoI CCATGG 1 cut(s) 1197
NdeII GATC 3 cut(s) 475, 613, 1442
NheI GCTAGC 1 cut(s) 304
NlaIV GGNNCC 4 cut(s) 532, 596, 624, 1329
NmuCI GTSAC 3 cut(s) 427, 512, 1274
NspI RCATGY 1 cut(s) 397
OliI CACNNNNGTG 1 cut(s) 248
PaeI GCATGC 1 cut(s) 397
PagI TCATGA 2 cut(s) 216, 292
PceI AGGCCT 1 cut(s) 920
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PfeI GAWTC 1 cut(s) 289
PflMI CCANNNNNTGG 2 cut(s) 140, 1287
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 2 cut(s) 405, 437
Ple19I CGATCG 1 cut(s) 478
PleI GAGTC 2 cut(s) 187, 884
PmaCI CACGTG 1 cut(s) 468
PmlI CACGTG 1 cut(s) 468
PpsI GAGTC 2 cut(s) 187, 884
Ppu21I YACGTR 1 cut(s) 468
PsiI TTATAA 1 cut(s) 1347
Psp6I CCWGG 1 cut(s) 115
PspCI CACGTG 1 cut(s) 468
PspFI CCCAGC 2 cut(s) 644, 683
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 4 cut(s) 532, 596, 624, 1329
PspPI GGNCC 5 cut(s) 119, 209, 370, 380, 622
PsrI GAACNNNNNNTAC 2 cut(s) 340, 372
PstNI CAGNNNCTG 1 cut(s) 983
PsuI RGATCY 1 cut(s) 613
PvuI CGATCG 1 cut(s) 478
RsaI GTAC 2 cut(s) 508, 887
RsaNI GTAC 2 cut(s) 507, 886
RseI CAYNNNNRTG 4 cut(s) 248, 551, 950, 1196
SaqAI TTAA 5 cut(s) 285, 540, 582, 1029, 1301
SatI GCNGC 2 cut(s) 404, 436
Sau3AI GATC 3 cut(s) 475, 613, 1442
Sau96I GGNCC 5 cut(s) 119, 209, 370, 380, 622
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 2 cut(s) 188, 884
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 5 cut(s) 169, 245, 879, 1076, 1410
SfaNI GCATC 4 cut(s) 94, 480, 490, 1567
SfcI CTRYAG 1 cut(s) 922
SinI GGWCC 2 cut(s) 119, 622
SmiMI CAYNNNNRTG 4 cut(s) 248, 551, 950, 1196
SmlI CTYRAG 2 cut(s) 1417, 1484
SmoI CTYRAG 2 cut(s) 1417, 1484
SpeI ACTAGT 1 cut(s) 509
SphI GCATGC 1 cut(s) 397
Sse9I AATT 4 cut(s) 588, 758, 783, 1266
SseBI AGGCCT 1 cut(s) 920
SsiI CCGC 6 cut(s) 170, 199, 403, 436, 734, 1019
SspI AATATT 1 cut(s) 1305
StuI AGGCCT 1 cut(s) 920
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 5 cut(s) 93, 609, 1002, 1197, 1438
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 3 cut(s) 470, 954, 1276
TaqI TCGA 6 cut(s) 79, 260, 313, 474, 768, 1362
TaqII GACCGA 1 cut(s) 1076
TasI AATT 4 cut(s) 588, 758, 783, 1266
TatI WGTACW 1 cut(s) 506
TauI GCSGC 2 cut(s) 406, 438
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 5 cut(s) 285, 540, 582, 1029, 1301
Tru9I TTAA 5 cut(s) 285, 540, 582, 1029, 1301
TscAI CASTG 5 cut(s) 141, 250, 392, 421, 832
TseFI GTSAC 3 cut(s) 427, 512, 1274
Tsp45I GTSAC 3 cut(s) 427, 512, 1274
TspDTI ATGAA 5 cut(s) 246, 347, 787, 927, 1357
TspRI CASTG 5 cut(s) 141, 250, 392, 421, 832
Van91I CCANNNNNTGG 2 cut(s) 140, 1287
VneI GTGCAC 1 cut(s) 165
VpaK11BI GGWCC 2 cut(s) 119, 622
XagI CCTNNNNNAGG 1 cut(s) 1569
XceI RCATGY 1 cut(s) 397
XcmI CCANNNNNNNNNTGG 2 cut(s) 123, 616
XmaJI CCTAGG 1 cut(s) 1438
ZrmI AGTACT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.