RLG00000024770

Glycerol-3-phosphate 2-O-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015009
Physical Location & Seq
Reverse (-)
38903395 .. 38906484
3090 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024770

Sequence Viewer

Length: 1488 bp
ATGGCACGGTGTCGATTTGAGCCAATTTCGAGATGTAGCACCGAGGGACGATCGAACCAGACAGTGGCTGCAGACCTCGACGGCACGCTCCTCGTGTCCCGAAGCGCTTTCCCTTATTTCATGCTCGTTGCTCTTGAAGCCGGCAGTCTATTAAGGGCACTTTTACTACTAGCATCCGTACCATTCGTATATTTCATCTACTTGTTCATATCAGAATCCGTAGCAATCAATACCTTCATCTTCATTTCTTTCGCCGGTCTGAAGCTCAAGGACATTGAGATGGTTGCGAGGTCCGTCTTGCCCAAATTTTACGCAGAAGACGTTCACCCGCAGACTTGGAAAGTGTTCAAGTCGTTCGGCAAGCGCTACATCATCACTGCCAATCCCAGAGTTCTTGTGGAGCCTTTTGTCAAGAGCTTTCTTGGTGCTGATAAGGTTCTTGGGACAGAGTTGGAAGTGACCAAGTCCGGGAGGGCTACTGGGTTTGTTCAAAAACCAGGTGTTCTAGTCGGAGAGCACAAAAGAGAAGCCATCGTCAAGGAATTCGGCGCTGACTTACCGGATTTGGGTCTCGGAGATAGAGAAACTGACCATGATTTTATGTCCATTTGCAAGGAAGGGTACATGGTCCCAAGGACAAAATGTGAGCCCTTGCCAAGAAACAAGCTTTTGAGCCCGGTTATATTTCATGAAGGCCGTTTGGTACAAAGACCTACTCCTTTGGTTGCCCTGTTGACCTTTTTATGGATGCCAATAGGTATAATCCTATCCATTTTAAGAGTCTACACCAACATCATACTGCCTGAGAGAATTGCTAGATACAACTATAAGCTTCTGGGGATTAATCTAATTGTTAAGGGCAACCCTCCACCACCACCTAAAAAGGGTCACCCTGGGGTTCTATTTATTTGCAACCATCGCACAGTTTTAGACCCTGTTGTAACAGCAGTGGCATTAGGAAGAAAAATTAGTTGTGTCACATACAGCATTAGCAAATTTTCCGAACTAATCTCTCCGATCAAGACTGTGGCATTGACAAGGGAAAGAGAGAAAGACGCGGCGAACATTAAGGCTTTGCTTGAGGAAGGTGACCTCGTGATCTGCCCTGAAGGAACTACTTGCAGAGAGCCATTTCTGTTGAGGTTCAGTGCGCTGTTTGCTGAGCTTACTGATAGGATTGTTCCGGTTGCAATTAACACCAAGCAAAGTGTGTATTACGGGACAACAGTCCGCGGACACAAATGGTTGGATCCTTATTTTGTGTTCATGAATCCAATGCCATCTTATGAGATCACGTTTTTAAATCAGCTACCAATGGAACTCACTTGCCAAGGTGGAAAATCGCCGTTTGAAGTGGCGAATTACATTCAGAGGGTATTGGCCGGGACTTTAGGGTTTGAGTGCACAAATTTGACCCGGAAGGATAAGTATTCTGCGATTGCTGGAACAGATGGTAGGGTTACATCATCCAACAAGGAAAAGGAATAG

Protein Analysis

496

Amino Acids

55.3

Weight (kDa)

9.17

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_RAM2_N PF23270 21 - 210 7.9e-67 Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD PF12710 23 - 201 2.4e-22 haloacid dehalogenase-like hydrolase
Acyltransferase PF01553 298 - 398 7.6e-08 Acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38110
fragaria_vesca FvH4_6g11850
malus_domestica MD04G1148700.v1.1
prunus_persica Prupe.6G272900_v2.0.a1
pyrus_communis pycom04g13420 pycom12g15400
rosa_chinensis RchiOBHm_Chr3g0463591
rosa_laevigata RLG00000024770
rosa_multiflora Rmu_sc0003267.1_g000019
rosa_roxburghii Rroxscaffold_6G00416410
rosa_rugosa Rorug03G0066100
rosa_samantha Rh3AG123200 Rh3BG128100 Rh3CG129800 Rh3DG128700
rosa_wichuraiana Rw3G010420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 64
AccI GTMKAC 1 cut(s) 783
AccII CGCG 2 cut(s) 1058, 1233
AciI CCGC 4 cut(s) 329, 1058, 1231, 1233
AclWI GGATC 2 cut(s) 1244, 1257
AcoI YGGCCR 1 cut(s) 1380
AcsI RAATTY 4 cut(s) 305, 542, 995, 1408
AcuI CTGAAG 2 cut(s) 281, 1128
AfaI GTAC 3 cut(s) 180, 623, 705
AfeI AGCGCT 2 cut(s) 106, 365
AfiI CCNNNNNNNGG 5 cut(s) 64, 468, 566, 744, 884
AgsI TTSAA 4 cut(s) 137, 349, 491, 1352
AjnI CCWGG 2 cut(s) 496, 892
AluBI AGCT 6 cut(s) 265, 417, 667, 832, 1165, 1309
AluI AGCT 6 cut(s) 265, 417, 667, 832, 1165, 1309
Alw21I GWGCWC 2 cut(s) 519, 1406
Alw26I GTCTC 1 cut(s) 575
Alw44I GTGCAC 1 cut(s) 1402
AlwI GGATC 2 cut(s) 1244, 1257
AlwNI CAGNNNCTG 1 cut(s) 68
Aor51HI AGCGCT 2 cut(s) 106, 365
AoxI GGCC 2 cut(s) 694, 1380
ApaLI GTGCAC 1 cut(s) 1402
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 4 cut(s) 305, 542, 995, 1408
AseI ATTAAT 1 cut(s) 843
Asp700I GAANNNNTTC 2 cut(s) 321, 344
AspLEI GCGC 4 cut(s) 107, 366, 551, 1153
AspS9I GGNCC 2 cut(s) 291, 628
AsuC2I CCSGG 4 cut(s) 469, 677, 1384, 1417
AsuHPI GGTGA 3 cut(s) 317, 881, 1100
AvaII GGWCC 2 cut(s) 291, 628
BaeGI GKGCMC 2 cut(s) 160, 1406
BamHI GGATCC 1 cut(s) 1249
BanII GRGCYC 2 cut(s) 651, 677
BauI CACGAG 2 cut(s) 92, 1094
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 519, 1406
BbvI GCAGC 1 cut(s) 55
BccI CCATC 5 cut(s) 274, 539, 924, 1288, 1445
BceAI ACGGC 3 cut(s) 97, 681, 1330
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 2 cut(s) 498, 894
BcnI CCSGG 4 cut(s) 469, 677, 1384, 1417
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 3 cut(s) 170, 506, 816
BfmI CTRYAG 1 cut(s) 69
BfoI RGCGCY 3 cut(s) 108, 367, 552
BisI GCNGC 2 cut(s) 69, 1059
BlpI GCTNAGC 1 cut(s) 1161
BlsI GCNGC 2 cut(s) 70, 1060
Bme1390I CCNGG 6 cut(s) 469, 498, 677, 894, 1384, 1417
Bme18I GGWCC 2 cut(s) 291, 628
BmgT120I GGNCC 2 cut(s) 291, 628
BmiI GGNNCC 3 cut(s) 402, 630, 1251
BmrFI CCNGG 6 cut(s) 469, 498, 677, 894, 1384, 1417
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 2 cut(s) 182, 738
BmuI ACTGGG 1 cut(s) 489
BoxI GACNNNNGTC 1 cut(s) 1226
BpiI GAAGAC 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 2 cut(s) 251, 1100
BpuMI CCSGG 4 cut(s) 469, 677, 1384, 1417
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 6 cut(s) 42, 632, 892, 893, 1231, 1330
BsaWI WCCGGW 2 cut(s) 559, 1183
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 468, 566, 744, 884
Bse118I RCCGGY 2 cut(s) 140, 254
Bse1I ACTGG 1 cut(s) 484
BseBI CCWGG 2 cut(s) 498, 894
BseDI CCNNGG 6 cut(s) 42, 632, 892, 893, 1231, 1330
BseGI GGATG 3 cut(s) 173, 753, 1466
BseLI CCNNNNNNNGG 5 cut(s) 64, 468, 566, 744, 884
BseMII CTCAG 2 cut(s) 795, 1152
BseNI ACTGG 1 cut(s) 484
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 2 cut(s) 160, 1406
BseXI GCAGC 1 cut(s) 55
Bsh1236I CGCG 2 cut(s) 1058, 1233
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 696, 1382
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 2 cut(s) 519, 1406
BsiSI CCGG 8 cut(s) 141, 255, 468, 560, 677, 1184, 1383, 1417
BslFI GGGAC 6 cut(s) 60, 82, 457, 614, 1234, 1399
BslI CCNNNNNNNGG 5 cut(s) 64, 468, 566, 744, 884
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 6 cut(s) 60, 82, 457, 614, 1234, 1399
BsnI GGCC 2 cut(s) 696, 1382
Bso31I GGTCTC 1 cut(s) 575
Bsp1286I GDGCHC 5 cut(s) 160, 519, 651, 677, 1406
Bsp143I GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
Bsp1720I GCTNAGC 1 cut(s) 1161
BspACI CCGC 4 cut(s) 329, 1058, 1231, 1233
BspANI GGCC 2 cut(s) 696, 1382
BspCNI CTCAG 2 cut(s) 796, 1153
BspFNI CGCG 2 cut(s) 1058, 1233
BspHI TCATGA 2 cut(s) 688, 1266
BspLI GGNNCC 3 cut(s) 402, 630, 1251
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 2 cut(s) 1244, 1257
BspTNI GGTCTC 1 cut(s) 575
BsrFI RCCGGY 2 cut(s) 140, 254
BsrI ACTGG 1 cut(s) 484
BssAI RCCGGY 2 cut(s) 140, 254
BssECI CCNNGG 6 cut(s) 42, 632, 892, 893, 1231, 1330
BssMI GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
BssSI CACGAG 2 cut(s) 92, 1094
BssT1I CCWWGG 2 cut(s) 632, 1330
Bst2BI CACGAG 2 cut(s) 92, 1094
Bst2UI CCWGG 2 cut(s) 498, 894
Bst4CI ACNGT 5 cut(s) 9, 64, 925, 1027, 1228
BstC8I GCNNGC 3 cut(s) 86, 142, 362
BstDEI CTNAG 2 cut(s) 804, 1161
BstDSI CCRYGG 1 cut(s) 1231
BstEII GGTNACC 2 cut(s) 887, 1088
BstF5I GGATG 3 cut(s) 173, 753, 1466
BstFNI CGCG 2 cut(s) 1058, 1233
BstH2I RGCGCY 3 cut(s) 108, 367, 552
BstHHI GCGC 4 cut(s) 107, 366, 551, 1153
BstKTI GATC 5 cut(s) 53, 1020, 1101, 1252, 1293
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 3 cut(s) 137, 918, 1157
BstNI CCWGG 2 cut(s) 498, 894
BstPAI GACNNNNGTC 1 cut(s) 1226
BstPI GGTNACC 2 cut(s) 887, 1088
BstSCI CCNGG 6 cut(s) 467, 496, 675, 892, 1382, 1415
BstSFI CTRYAG 1 cut(s) 69
BstSLI GKGCMC 2 cut(s) 160, 1406
BstUI CGCG 2 cut(s) 1058, 1233
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 1249
BstYI RGATCY 1 cut(s) 1249
BsuRI GGCC 2 cut(s) 696, 1382
BtgI CCRYGG 1 cut(s) 1231
BtgZI GCGATG 1 cut(s) 902
BtsCI GGATG 3 cut(s) 173, 753, 1466
BtsI GCAGTG 2 cut(s) 375, 954
BtsIMutI CAGTG 4 cut(s) 69, 375, 954, 1153
Cac8I GCNNGC 3 cut(s) 86, 142, 362
CaiI CAGNNNCTG 1 cut(s) 68
CciI TCATGA 2 cut(s) 688, 1266
CfoI GCGC 4 cut(s) 107, 366, 551, 1153
Cfr10I RCCGGY 2 cut(s) 140, 254
Cfr13I GGNCC 2 cut(s) 291, 628
Cfr42I CCGCGG 1 cut(s) 1234
CseI GACGC 1 cut(s) 1064
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 3 cut(s) 179, 622, 704
CviAII CATG 5 cut(s) 121, 593, 625, 689, 1267
CviQI GTAC 3 cut(s) 179, 622, 704
DdeI CTNAG 2 cut(s) 804, 1161
DpnI GATC 5 cut(s) 52, 1019, 1100, 1251, 1292
DpnII GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
DraI TTTAAA 1 cut(s) 1302
EaeI YGGCCR 1 cut(s) 1380
Eco130I CCWWGG 2 cut(s) 632, 1330
Eco24I GRGCYC 2 cut(s) 651, 677
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 2 cut(s) 291, 628
Eco47III AGCGCT 2 cut(s) 106, 365
Eco57I CTGAAG 2 cut(s) 281, 1128
Eco91I GGTNACC 2 cut(s) 887, 1088
EcoO65I GGTNACC 2 cut(s) 887, 1088
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 496, 892
EcoT14I CCWWGG 2 cut(s) 632, 1330
EcoT38I GRGCYC 2 cut(s) 651, 677
ErhI CCWWGG 2 cut(s) 632, 1330
FaeI CATG 5 cut(s) 124, 596, 628, 692, 1270
FaqI GGGAC 6 cut(s) 60, 82, 457, 614, 1234, 1399
FatI CATG 5 cut(s) 120, 592, 624, 688, 1266
FauI CCCGC 1 cut(s) 336
FblI GTMKAC 1 cut(s) 783
Fnu4HI GCNGC 2 cut(s) 69, 1059
FokI GGATG 3 cut(s) 160, 760, 1453
FriOI GRGCYC 2 cut(s) 651, 677
Fsp4HI GCNGC 2 cut(s) 69, 1059
FspBI CTAG 3 cut(s) 170, 506, 816
GlaI GCGC 4 cut(s) 106, 365, 550, 1152
GluI GCNGC 2 cut(s) 69, 1059
HaeII RGCGCY 3 cut(s) 108, 367, 552
HaeIII GGCC 2 cut(s) 696, 1382
HapII CCGG 8 cut(s) 141, 255, 468, 560, 677, 1184, 1383, 1417
HgaI GACGC 1 cut(s) 1064
HhaI GCGC 4 cut(s) 107, 366, 551, 1153
Hin1II CATG 5 cut(s) 124, 596, 628, 692, 1270
Hin6I GCGC 4 cut(s) 105, 364, 549, 1151
HinP1I GCGC 4 cut(s) 105, 364, 549, 1151
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HindIII AAGCTT 2 cut(s) 665, 830
HinfI GANTC 3 cut(s) 215, 780, 1270
HpaII CCGG 8 cut(s) 141, 255, 468, 560, 677, 1184, 1383, 1417
HphI GGTGA 3 cut(s) 317, 881, 1100
Hpy166II GTNNAC 4 cut(s) 325, 735, 784, 1404
Hpy188I TCNGA 7 cut(s) 214, 261, 512, 575, 1003, 1017, 1371
Hpy188III TCNNGA 8 cut(s) 30, 99, 134, 412, 689, 1021, 1096, 1267
Hpy8I GTNNAC 4 cut(s) 325, 735, 784, 1404
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 6 cut(s) 244, 611, 686, 1079, 1103, 1414
HpyCH4III ACNGT 5 cut(s) 9, 64, 925, 1027, 1228
HpyCH4IV ACGT 2 cut(s) 321, 1295
HpyCH4V TGCA 6 cut(s) 71, 612, 912, 1122, 1190, 1404
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 918, 1157
HpyF3I CTNAG 2 cut(s) 804, 1161
HpySE526I ACGT 2 cut(s) 321, 1295
Hsp92II CATG 5 cut(s) 124, 596, 628, 692, 1270
HspAI GCGC 4 cut(s) 105, 364, 549, 1151
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
KspI CCGCGG 1 cut(s) 1234
Kzo9I GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
LmnI GCTCC 2 cut(s) 93, 400
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 2 cut(s) 182, 738
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 3 cut(s) 170, 506, 816
MaeII ACGT 2 cut(s) 321, 1295
MaeIII GTNAC 6 cut(s) 457, 887, 940, 976, 1088, 1459
MalI GATC 5 cut(s) 52, 1019, 1100, 1251, 1292
MboI GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
MboII GAAGA 3 cut(s) 232, 329, 972
MflI RGATCY 1 cut(s) 1249
MhlI GDGCHC 5 cut(s) 160, 519, 651, 677, 1406
MlyI GAGTC 1 cut(s) 789
MmeI TCCRAC 3 cut(s) 432, 490, 1227
MroNI GCCGGC 1 cut(s) 140
MroXI GAANNNNTTC 2 cut(s) 321, 344
MseI TTAA 7 cut(s) 152, 776, 843, 855, 1068, 1194, 1301
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 1233
MspI CCGG 8 cut(s) 141, 255, 468, 560, 677, 1184, 1383, 1417
MspR9I CCNGG 6 cut(s) 469, 498, 677, 894, 1384, 1417
MvaI CCWGG 2 cut(s) 498, 894
MvnI CGCG 2 cut(s) 1058, 1233
MwoI GCNNNNNNNGC 3 cut(s) 137, 918, 1157
NaeI GCCGGC 1 cut(s) 142
NciI CCSGG 4 cut(s) 469, 677, 1384, 1417
NdeII GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 5 cut(s) 124, 596, 628, 692, 1270
NlaIV GGNNCC 3 cut(s) 402, 630, 1251
NmuCI GTSAC 4 cut(s) 457, 887, 976, 1088
PagI TCATGA 2 cut(s) 688, 1266
PasI CCCWGGG 1 cut(s) 893
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 142
PdmI GAANNNNTTC 2 cut(s) 321, 344
PfeI GAWTC 2 cut(s) 215, 1270
PflFI GACNNNGTC 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 64
PfoI TCCNGGA 1 cut(s) 467
PkrI GCNGC 2 cut(s) 70, 1060
Ple19I CGATCG 1 cut(s) 53
PleI GAGTC 1 cut(s) 788
PpsI GAGTC 1 cut(s) 788
PshAI GACNNNNGTC 1 cut(s) 1226
PshBI ATTAAT 1 cut(s) 843
Psp6I CCWGG 2 cut(s) 496, 892
PspEI GGTNACC 2 cut(s) 887, 1088
PspGI CCWGG 2 cut(s) 496, 892
PspN4I GGNNCC 3 cut(s) 402, 630, 1251
PspPI GGNCC 2 cut(s) 291, 628
PstI CTGCAG 1 cut(s) 73
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 1 cut(s) 1249
PsyI GACNNNGTC 1 cut(s) 463
PvuI CGATCG 1 cut(s) 53
RsaI GTAC 3 cut(s) 180, 623, 705
RsaNI GTAC 3 cut(s) 179, 622, 704
RseI CAYNNNNRTG 1 cut(s) 278
SacII CCGCGG 1 cut(s) 1234
SaqAI TTAA 7 cut(s) 152, 776, 843, 855, 1068, 1194, 1301
SatI GCNGC 2 cut(s) 69, 1059
Sau3AI GATC 5 cut(s) 50, 1017, 1098, 1249, 1290
Sau96I GGNCC 2 cut(s) 291, 628
SchI GAGTC 1 cut(s) 789
ScrFI CCNGG 6 cut(s) 469, 498, 677, 894, 1384, 1417
SduI GDGCHC 5 cut(s) 160, 519, 651, 677, 1406
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 2 cut(s) 182, 738
SfcI CTRYAG 1 cut(s) 69
Sfr303I CCGCGG 1 cut(s) 1234
SgrBI CCGCGG 1 cut(s) 1234
SinI GGWCC 2 cut(s) 291, 628
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 2 cut(s) 266, 1079
SmoI CTYRAG 2 cut(s) 266, 1079
SsiI CCGC 4 cut(s) 329, 1058, 1231, 1233
SspMI CTAG 3 cut(s) 170, 506, 816
StyD4I CCNGG 6 cut(s) 467, 496, 675, 892, 1382, 1415
StyI CCWWGG 2 cut(s) 632, 1330
TaaI ACNGT 5 cut(s) 9, 64, 925, 1027, 1228
TaiI ACGT 2 cut(s) 324, 1298
TaqI TCGA 4 cut(s) 13, 29, 53, 78
TauI GCSGC 1 cut(s) 1061
TfiI GAWTC 2 cut(s) 215, 1270
Tru1I TTAA 7 cut(s) 152, 776, 843, 855, 1068, 1194, 1301
Tru9I TTAA 7 cut(s) 152, 776, 843, 855, 1068, 1194, 1301
TscAI CASTG 4 cut(s) 69, 382, 954, 1153
TseFI GTSAC 4 cut(s) 457, 887, 976, 1088
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 4 cut(s) 457, 887, 976, 1088
TspDTI ATGAA 9 cut(s) 109, 184, 196, 226, 232, 677, 705, 1255, 1283
TspGWI ACGGA 3 cut(s) 166, 208, 283
TspRI CASTG 4 cut(s) 69, 382, 954, 1153
Tth111I GACNNNGTC 1 cut(s) 463
Van91I CCANNNNNTGG 1 cut(s) 64
VneI GTGCAC 1 cut(s) 1402
VpaK11BI GGWCC 2 cut(s) 291, 628
VspI ATTAAT 1 cut(s) 843
XapI RAATTY 4 cut(s) 305, 542, 995, 1408
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 783
XmnI GAANNNNTTC 2 cut(s) 321, 344
XspI CTAG 3 cut(s) 170, 506, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.