RLG00000028320

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018960
Physical Location & Seq
Forward (+)
21381100 .. 21381882
783 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028320

Sequence Viewer

Length: 783 bp
ATGGGCAATGATGAAGAATCAGTTCATGGTGGTTGCGAAAGTGGATCCGATAGCGATGGCTGTGAAGGACTACCTGAGGCTGATGTTAATAAAGAGAGCAACCTAAGTGATGAGGCCGATCGTGTTTCGATTGATGACGCTTCTCAAAATATTGATGCAGAGGTCTTGAGGATCAAGGAAATTTATGAAAACAGTGATAAGGAAAAGGCTAATGACGCTTCGATGCTCGAGTCTCTGACGAGACTAAAGATGATGACTATGACTGTGGAGACTCTTGAGGCAACCGCAATTGGTAAAGTTGTTAGTGGTTTAAAAAAGCATGGATCCAAGCAGATTCGTGATCTTGCTCGAACACTTGTTGATGGGTGGAAAGTCGTGGTAAGTGAATATTTGGCTACCCAAAATTCTAAACAGTCCTCTTCAGTTGTTCTTAGCAAACAAAACAAGAATGAACAACTAGTGATCAAGAAACAAGAAGTTGTAGCTGTGAAGCCAAATAAGGCCTCAAACAACGATGAAGAAAAGATGCAGCGCTTGGATGATGAAGTTTCTGCGCAAGCGAAAATCGAAGCCACAAAAAGGAAACTTCATGAGCGTTATGAGCAAATTGAGAACACCAAGAAGCAAAGGATGGTTCAAGTCTTGGATGATCTCCCCAAGCAAGGGCCTAAAGTTGGCTCCCACACTACTAATCTACATGGAAAGAAACCTCGTTCAGCACATGGACGGATATCCGATAAGTTGCGCAGATTAAAAAAGGAAGCTGGTGTGGTCGGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

261

Amino Acids

28.81

Weight (kDa)

7.03

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 78 - 125 6.6e-14 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018960)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0352691
rosa_laevigata RLG00000028320
rosa_multiflora Rmu_sc0039102.1_g000001 Rmu_sc0039103.1_g000001
rosa_roxburghii Rroxscaffold_4G00302470
rosa_rugosa Rorug01G0231000
rosa_samantha Rh1AG244100 Rh1BG214800 Rh1CG228100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 555, 746
AciI CCGC 1 cut(s) 285
AclWI GGATC 5 cut(s) 39, 52, 179, 318, 331
AcsI RAATTY 2 cut(s) 180, 403
AcuI CTGAAG 1 cut(s) 405
AfeI AGCGCT 1 cut(s) 533
AfiI CCNNNNNNNGG 4 cut(s) 579, 662, 663, 674
AgsI TTSAA 1 cut(s) 638
AhlI ACTAGT 1 cut(s) 457
AluBI AGCT 2 cut(s) 485, 764
AluI AGCT 2 cut(s) 485, 764
Alw26I GTCTC 3 cut(s) 235, 237, 263
AlwI GGATC 5 cut(s) 39, 52, 179, 318, 331
Ama87I CYCGRG 1 cut(s) 227
Aor51HI AGCGCT 1 cut(s) 533
AoxI GGCC 3 cut(s) 114, 501, 665
ApeKI GCWGC 1 cut(s) 529
ApoI RAATTY 2 cut(s) 180, 403
Asp700I GAANNNNTTC 1 cut(s) 21
AspLEI GCGC 3 cut(s) 534, 556, 747
AspS9I GGNCC 1 cut(s) 665
AvaI CYCGRG 1 cut(s) 227
AxyI CCTNAGG 1 cut(s) 75
BamHI GGATCC 2 cut(s) 44, 323
BbvI GCAGC 1 cut(s) 541
BccI CCATC 3 cut(s) 50, 356, 625
BclI TGATCA 1 cut(s) 462
BcoDI GTCTC 3 cut(s) 235, 237, 263
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 1 cut(s) 458
BfoI RGCGCY 1 cut(s) 535
BisI GCNGC 1 cut(s) 530
BlsI GCNGC 1 cut(s) 531
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 3 cut(s) 46, 325, 679
BmsI GCATC 3 cut(s) 145, 213, 516
BpuEI CTTGAG 2 cut(s) 187, 296
Bsc4I CCNNNNNNNGG 4 cut(s) 579, 662, 663, 674
Bse21I CCTNAGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 13
BseGI GGATG 3 cut(s) 544, 636, 652
BseLI CCNNNNNNNGG 4 cut(s) 579, 662, 663, 674
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 541
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 3 cut(s) 116, 503, 667
BsiEI CGRYCG 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 227
BslI CCNNNNNNNGG 4 cut(s) 579, 662, 663, 674
BsmAI GTCTC 3 cut(s) 235, 237, 263
BsnI GGCC 3 cut(s) 116, 503, 667
BsoBI CYCGRG 1 cut(s) 227
Bsp143I GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
BspACI CCGC 1 cut(s) 285
BspANI GGCC 3 cut(s) 116, 503, 667
BspCNI CTCAG 1 cut(s) 67
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 3 cut(s) 46, 325, 679
BspPI GGATC 5 cut(s) 39, 52, 179, 318, 331
BsrDI GCAATG 1 cut(s) 13
BssMI GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
Bst4CI ACNGT 3 cut(s) 194, 265, 414
Bst6I CTCTTC 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 3 cut(s) 75, 104, 431
BstF5I GGATG 3 cut(s) 544, 636, 652
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 3 cut(s) 534, 556, 747
BstKTI GATC 7 cut(s) 47, 121, 174, 326, 343, 465, 652
BstMAI GTCTC 3 cut(s) 235, 237, 263
BstMBI GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 2 cut(s) 215, 601
BstV1I GCAGC 1 cut(s) 541
BstX2I RGATCY 2 cut(s) 44, 323
BstYI RGATCY 2 cut(s) 44, 323
Bsu36I CCTNAGG 1 cut(s) 75
BsuRI GGCC 3 cut(s) 116, 503, 667
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 3 cut(s) 544, 636, 652
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 558
CciI TCATGA 1 cut(s) 589
CfoI GCGC 3 cut(s) 534, 556, 747
Cfr13I GGNCC 1 cut(s) 665
CseI GACGC 2 cut(s) 146, 224
CviAII CATG 5 cut(s) 26, 320, 590, 698, 722
DdeI CTNAG 3 cut(s) 75, 104, 431
DpnI GATC 7 cut(s) 46, 120, 173, 325, 342, 464, 651
DpnII GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
DraI TTTAAA 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 424
Eco147I AGGCCT 1 cut(s) 503
Eco32I GATATC 1 cut(s) 732
Eco47III AGCGCT 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 405
Eco81I CCTNAGG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 665
EcoRV GATATC 1 cut(s) 732
FaeI CATG 5 cut(s) 29, 323, 593, 701, 725
FaiI YATR 8 cut(s) 27, 186, 260, 321, 591, 600, 699, 723
FatI CATG 5 cut(s) 25, 319, 589, 697, 721
FbaI TGATCA 1 cut(s) 462
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 3 cut(s) 551, 643, 659
Fsp4HI GCNGC 1 cut(s) 530
FspBI CTAG 1 cut(s) 458
FspI TGCGCA 2 cut(s) 555, 746
GlaI GCGC 3 cut(s) 533, 555, 746
GluI GCNGC 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 535
HaeIII GGCC 3 cut(s) 116, 503, 667
HgaI GACGC 2 cut(s) 146, 224
HhaI GCGC 3 cut(s) 534, 556, 747
Hin1II CATG 5 cut(s) 29, 323, 593, 701, 725
Hin6I GCGC 3 cut(s) 532, 554, 745
HinP1I GCGC 3 cut(s) 532, 554, 745
HinfI GANTC 4 cut(s) 17, 230, 271, 334
Hpy188I TCNGA 3 cut(s) 49, 237, 736
Hpy188III TCNNGA 5 cut(s) 166, 275, 338, 466, 590
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 3 cut(s) 194, 265, 414
HpyCH4V TGCA 2 cut(s) 158, 529
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 601
HpyF3I CTNAG 3 cut(s) 75, 104, 431
Hsp92II CATG 5 cut(s) 29, 323, 593, 701, 725
HspAI GCGC 3 cut(s) 532, 554, 745
Ksp22I TGATCA 1 cut(s) 462
Kzo9I GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
LmnI GCTCC 1 cut(s) 683
LpnPI CCDG 2 cut(s) 87, 750
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 3 cut(s) 145, 213, 516
MaeI CTAG 1 cut(s) 458
MalI GATC 7 cut(s) 46, 120, 173, 325, 342, 464, 651
MboI GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
MboII GAAGA 3 cut(s) 26, 411, 530
MfeI CAATTG 1 cut(s) 288
MflI RGATCY 2 cut(s) 44, 323
MluCI AATT 4 cut(s) 180, 288, 403, 606
MlyI GAGTC 2 cut(s) 239, 265
MnlI CCTC 8 cut(s) 70, 106, 154, 162, 271, 427, 514, 720
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 3 cut(s) 87, 311, 752
MunI CAATTG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 215, 601
NdeII GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
NlaIII CATG 5 cut(s) 29, 323, 593, 701, 725
NlaIV GGNNCC 3 cut(s) 46, 325, 679
NsbI TGCGCA 2 cut(s) 555, 746
PaeR7I CTCGAG 1 cut(s) 227
PagI TCATGA 1 cut(s) 589
PceI AGGCCT 1 cut(s) 503
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 2 cut(s) 17, 334
PkrI GCNGC 1 cut(s) 531
Ple19I CGATCG 1 cut(s) 121
PleI GAGTC 2 cut(s) 238, 265
PpsI GAGTC 2 cut(s) 238, 265
PspN4I GGNNCC 3 cut(s) 46, 325, 679
PspPI GGNCC 1 cut(s) 665
PspXI VCTCGAGB 1 cut(s) 227
PsuI RGATCY 2 cut(s) 44, 323
PvuI CGATCG 1 cut(s) 121
SaqAI TTAA 3 cut(s) 87, 311, 752
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 7 cut(s) 44, 118, 171, 323, 340, 462, 649
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 2 cut(s) 239, 265
SetI ASST 6 cut(s) 76, 105, 165, 487, 712, 766
SfaNI GCATC 3 cut(s) 145, 213, 516
Sfr274I CTCGAG 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 227
SmlI CTYRAG 3 cut(s) 166, 227, 275
SmoI CTYRAG 3 cut(s) 166, 227, 275
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 4 cut(s) 180, 288, 403, 606
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 1 cut(s) 285
SspI AATATT 2 cut(s) 151, 389
SspMI CTAG 1 cut(s) 458
StuI AGGCCT 1 cut(s) 503
TaaI ACNGT 3 cut(s) 194, 265, 414
TaqI TCGA 5 cut(s) 128, 221, 228, 349, 567
TasI AATT 4 cut(s) 180, 288, 403, 606
TfiI GAWTC 2 cut(s) 17, 334
Tru1I TTAA 3 cut(s) 87, 311, 752
Tru9I TTAA 3 cut(s) 87, 311, 752
TscAI CASTG 1 cut(s) 199
TseI GCWGC 1 cut(s) 529
TspDTI ATGAA 7 cut(s) 14, 27, 201, 465, 531, 558, 578
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 1 cut(s) 199
XapI RAATTY 2 cut(s) 180, 403
XhoI CTCGAG 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 21
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.