RLG00000029274
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0024429
Physical Location & Seq
Forward (+)
35817044 .. 35818434
1391 bp
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UTR
Exon/CDS
Intron
RLM00000029274

Sequence Viewer

Length: 1314 bp
ATGGATGATAATAATCTGGGAAATGAGGATGGTGACTTGAATTTTCTCTCTTCTCTGGTTAATTGTACCAGTTTAGAACTTCTGGATATTAGTCAGAATAACTTTCAAGGAGTGCTACCTGAATCTATCAGCAATCTCTCAACAAAGCTCAAGGTAATGAGCTTAAGAAGGAATCAGCTAAGCGGAAGCATTCCCATTGGGATTGGAAATCTCCTCAACTTGGGAGTAGTAAGCTTCGAGGCAAACCTATTGACAGGCACCATACCAAATTCAGTATGTAAGATAAGTAGATTGTACAATCTGTTTTTAAGTTATAATCAGCTATCAGGTACTATCCCATCTTGTCTGGGAAACTTAACTTTGTTAAGTAGCTTAGTTCTCAAGTCAAACAACTTGCAAGGTGGCATACCACAAAGTCTTGGTCAGTGCAGGAATTTGTTAGCTTTGGTTCTTTCTCAAAATAATCTTAGTGGTCCTATTCCAATAGAAGTCATCCGTTTACCATCCCTGTCACAAGTTTTGGATTTATCCAGTAACCATTTTAGTGGTTATATTCCCATTGAAGTAGGTTTTTTGTCTAAACTTGCTTCCTTGAATCTTGCTGAAAATAGATTATCTGGTGATGTTCCAGGGATTCAAGATCTTGACCTCTCTCATAACTCCTTCTCTGGAACAATCCCTGATTATTTGCAGAGTTTCCCCTTCTTGCAGCATTTGAACCTCTCATTTAATGATTTTCGAGGTGAAGTACCCATACAAGGAGTTTTCGGGAATACAAGCGCAGTTTCTCTCCTGGGAAACAGACGACTTTGTGGAGGTGTATCTCAGTTAAGATTGTCTAAATGTAGGTACTTGCCTAAGACAGATAAAGCATTATTGTATAGGCTGGCTATTATCACAATTGCTTCTGGGGCCATTATTTGGTCGGTGTACTTTGTGCTCCACTATAGATCAAGAAATTCTGGAGTTAAGTCAGCTTCAGTATCATCATTGGATGTTTCCTTCTTAAAATTGTCATACGGCGATCTCCTGAAAGCAACTGAAGGCTTTTCGTCTCGAAATTTGATTGGTTCCGGTAGCTCTGGTTCTGTATACTGGGGAGTTCTTGATCAACATGAAGAAAGAATTGTTGCTGTGAAAGTACTCAATCTTCAGAGTTCAAGAGCTTCTAGAAGTTTCATATCTGAATGTGAAGTGTTGAAAAGCATAAGGCACCGGAATCTTGTCAGACTAGTGACTGCTTGCTCAAGTATTGATTTTCAAGGGAACGATTCAAAGCTTTGGTTTTTGAGTTCATGTTGGATGGAAATCTAG

Protein Analysis

438

Amino Acids

47.64

Weight (kDa)

8.53

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 352 - 417 5e-09 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 354 - 416 3.8e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024429)

Species Orthologous Gene IDs
malus_domestica MD00G1043900.v1.1
rosa_laevigata RLG00000029274
rosa_multiflora Rmu_co8430541.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 315
AccB1I GGYRCC 2 cut(s) 257, 1212
AccB7I CCANNNNNTGG 1 cut(s) 921
AccI GTMKAC 1 cut(s) 1092
AciI CCGC 1 cut(s) 183
AcsI RAATTY 5 cut(s) 40, 268, 433, 958, 1060
AcuI CTGAAG 3 cut(s) 963, 1062, 1136
AfaI GTAC 7 cut(s) 67, 296, 331, 750, 851, 932, 1143
AfiI CCNNNNNNNGG 3 cut(s) 220, 758, 921
AflII CTTAAG 1 cut(s) 163
AhlI ACTAGT 1 cut(s) 1231
AjnI CCWGG 2 cut(s) 628, 792
Alw21I GWGCWC 1 cut(s) 942
Alw26I GTCTC 1 cut(s) 1059
AoxI GGCC 1 cut(s) 912
ApeKI GCWGC 1 cut(s) 709
ApoI RAATTY 5 cut(s) 40, 268, 433, 958, 1060
ArsI GACNNNNNNTTYG 2 cut(s) 406, 438
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 2 cut(s) 473, 912
AsuHPI GGTGA 3 cut(s) 44, 632, 755
AvaII GGWCC 1 cut(s) 473
BanI GGYRCC 2 cut(s) 257, 1212
BarI GAAGNNNNNNTAC 4 cut(s) 738, 770, 1134, 1166
Bbv12I GWGCWC 1 cut(s) 942
BbvI GCAGC 1 cut(s) 721
BccI CCATC 4 cut(s) 23, 346, 511, 1297
BceAI ACGGC 1 cut(s) 1036
BciT130I CCWGG 2 cut(s) 630, 794
BclI TGATCA 1 cut(s) 1108
BcoDI GTCTC 1 cut(s) 1059
BcuI ACTAGT 1 cut(s) 1231
BfaI CTAG 3 cut(s) 1170, 1232, 1312
BfmI CTRYAG 1 cut(s) 946
BfrI CTTAAG 1 cut(s) 163
BglII AGATCT 1 cut(s) 640
BisI GCNGC 1 cut(s) 710
BlpI GCTNAGC 1 cut(s) 179
BlsI GCNGC 1 cut(s) 711
BmcAI AGTACT 1 cut(s) 1143
Bme1390I CCNGG 2 cut(s) 630, 794
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 2 cut(s) 473, 912
BmiI GGNNCC 4 cut(s) 259, 913, 1072, 1214
BmrFI CCNGG 2 cut(s) 630, 794
BmrI ACTGGG 1 cut(s) 1105
BmuI ACTGGG 1 cut(s) 1105
BpmI CTGGAG 1 cut(s) 984
Bpu1102I GCTNAGC 1 cut(s) 179
BpuEI CTTGAG 3 cut(s) 134, 365, 1231
BsaBI GATNNNNATC 2 cut(s) 12, 1307
BsaJI CCNNGG 2 cut(s) 629, 793
BsaWI WCCGGW 2 cut(s) 1073, 1215
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 758, 921
Bse1I ACTGG 3 cut(s) 69, 531, 1100
Bse8I GATNNNNATC 2 cut(s) 12, 1307
BseBI CCWGG 2 cut(s) 630, 794
BseDI CCNNGG 2 cut(s) 629, 793
BseGI GGATG 6 cut(s) 10, 34, 492, 503, 1000, 1308
BseJI GATNNNNATC 2 cut(s) 12, 1307
BseLI CCNNNNNNNGG 3 cut(s) 220, 758, 921
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 3 cut(s) 69, 531, 1100
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 721
BsgI GTGCAG 1 cut(s) 448
BshFI GGCC 1 cut(s) 914
BshNI GGYRCC 2 cut(s) 257, 1212
BsiHKAI GWGCWC 1 cut(s) 942
BsiSI CCGG 2 cut(s) 1074, 1216
BslI CCNNNNNNNGG 3 cut(s) 220, 758, 921
BsmAI GTCTC 1 cut(s) 1059
BsmBI CGTCTC 1 cut(s) 1059
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 1 cut(s) 914
Bsp1286I GDGCHC 1 cut(s) 942
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 4 cut(s) 640, 950, 1024, 1108
Bsp1720I GCTNAGC 1 cut(s) 179
BspACI CCGC 1 cut(s) 183
BspANI GGCC 1 cut(s) 914
BspCNI CTCAG 1 cut(s) 838
BspLI GGNNCC 4 cut(s) 259, 913, 1072, 1214
BspT107I GGYRCC 2 cut(s) 257, 1212
BspTI CTTAAG 1 cut(s) 163
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 3 cut(s) 69, 531, 1100
BssECI CCNNGG 2 cut(s) 629, 793
BssMI GATC 4 cut(s) 640, 950, 1024, 1108
BssNAI GTATAC 1 cut(s) 1093
Bst1107I GTATAC 1 cut(s) 1093
Bst2UI CCWGG 2 cut(s) 630, 794
Bst6I CTCTTC 1 cut(s) 55
BstAFI CTTAAG 1 cut(s) 163
BstAUI TGTACA 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 888, 1243
BstDEI CTNAG 5 cut(s) 179, 373, 467, 825, 858
BstF5I GGATG 6 cut(s) 10, 34, 492, 503, 1000, 1308
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 4 cut(s) 643, 953, 1027, 1111
BstMAI GTCTC 1 cut(s) 1059
BstMBI GATC 4 cut(s) 640, 950, 1024, 1108
BstMWI GCNNNNNNNGC 1 cut(s) 911
BstNI CCWGG 2 cut(s) 630, 794
BstSCI CCNGG 2 cut(s) 628, 792
BstSFI CTRYAG 1 cut(s) 946
BstV1I GCAGC 1 cut(s) 721
BstX2I RGATCY 1 cut(s) 640
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 1 cut(s) 640
BstZ17I GTATAC 1 cut(s) 1093
BsuRI GGCC 1 cut(s) 914
BtsCI GGATG 6 cut(s) 10, 34, 492, 503, 1000, 1308
BtsIMutI CAGTG 1 cut(s) 431
Cac8I GCNNGC 2 cut(s) 888, 1243
CfoI GCGC 1 cut(s) 782
Cfr13I GGNCC 2 cut(s) 473, 912
Csp6I GTAC 7 cut(s) 66, 295, 330, 749, 850, 931, 1142
CviAII CATG 2 cut(s) 1115, 1296
CviQI GTAC 7 cut(s) 66, 295, 330, 749, 850, 931, 1142
DdeI CTNAG 5 cut(s) 179, 373, 467, 825, 858
DpnI GATC 4 cut(s) 642, 952, 1026, 1110
DpnII GATC 4 cut(s) 640, 950, 1024, 1108
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 473
Eco57I CTGAAG 3 cut(s) 963, 1062, 1136
EcoRII CCWGG 2 cut(s) 628, 792
Esp3I CGTCTC 1 cut(s) 1059
FaeI CATG 2 cut(s) 1118, 1299
FatI CATG 2 cut(s) 1114, 1295
FbaI TGATCA 1 cut(s) 1108
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 1 cut(s) 710
FokI GGATG 5 cut(s) 17, 41, 479, 490, 1007
Fsp4HI GCNGC 1 cut(s) 710
FspBI CTAG 3 cut(s) 1170, 1232, 1312
GlaI GCGC 1 cut(s) 781
GluI GCNGC 1 cut(s) 710
GsuI CTGGAG 1 cut(s) 984
HaeIII GGCC 1 cut(s) 914
HapII CCGG 2 cut(s) 1074, 1216
HhaI GCGC 1 cut(s) 782
Hin1II CATG 2 cut(s) 1118, 1299
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HindIII AAGCTT 2 cut(s) 232, 1277
HinfI GANTC 6 cut(s) 122, 172, 595, 634, 1219, 1271
HpaII CCGG 2 cut(s) 1074, 1216
HphI GGTGA 3 cut(s) 44, 632, 755
Hpy166II GTNNAC 3 cut(s) 500, 931, 1093
Hpy188I TCNGA 4 cut(s) 96, 1155, 1186, 1229
Hpy8I GTNNAC 3 cut(s) 500, 931, 1093
HpyAV CCTTC 5 cut(s) 162, 673, 712, 1012, 1037
HpyCH4V TGCA 4 cut(s) 397, 429, 691, 709
HpyF10VI GCNNNNNNNGC 1 cut(s) 911
HpyF3I CTNAG 5 cut(s) 179, 373, 467, 825, 858
Hsp92II CATG 2 cut(s) 1118, 1299
HspAI GCGC 1 cut(s) 780
Ksp22I TGATCA 1 cut(s) 1108
Kzo9I GATC 4 cut(s) 640, 950, 1024, 1108
LmnI GCTCC 1 cut(s) 945
Lsp1109I GCAGC 1 cut(s) 721
MaeI CTAG 3 cut(s) 1170, 1232, 1312
MaeIII GTNAC 4 cut(s) 32, 510, 533, 1234
MalI GATC 4 cut(s) 642, 952, 1026, 1110
MboI GATC 4 cut(s) 640, 950, 1024, 1108
MboII GAAGA 3 cut(s) 42, 1130, 1142
MfeI CAATTG 1 cut(s) 900
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 942
MluCI AATT 9 cut(s) 40, 61, 268, 433, 900, 958, 1010, 1060, 1125
MmeI TCCRAC 1 cut(s) 1280
MnlI CCTC 7 cut(s) 19, 224, 232, 659, 731, 734, 809
MseI TTAA 9 cut(s) 60, 164, 308, 356, 365, 729, 830, 969, 1007
MslI CAYNNNNRTG 1 cut(s) 543
MspCI CTTAAG 1 cut(s) 163
MspI CCGG 2 cut(s) 1074, 1216
MspR9I CCNGG 2 cut(s) 630, 794
MunI CAATTG 1 cut(s) 900
Mva1269I GAATGC 1 cut(s) 189
MvaI CCWGG 2 cut(s) 630, 794
MwoI GCNNNNNNNGC 1 cut(s) 911
NdeII GATC 4 cut(s) 640, 950, 1024, 1108
NlaIII CATG 2 cut(s) 1118, 1299
NlaIV GGNNCC 4 cut(s) 259, 913, 1072, 1214
NmuCI GTSAC 3 cut(s) 32, 510, 1234
PctI GAATGC 1 cut(s) 189
PfeI GAWTC 6 cut(s) 122, 172, 595, 634, 1219, 1271
PflMI CCANNNNNTGG 1 cut(s) 921
PkrI GCNGC 1 cut(s) 711
PsiI TTATAA 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 628, 792
PspGI CCWGG 2 cut(s) 628, 792
PspN4I GGNNCC 4 cut(s) 259, 913, 1072, 1214
PspPI GGNCC 2 cut(s) 473, 912
PsrI GAACNNNNNNTAC 2 cut(s) 1069, 1101
PsuI RGATCY 1 cut(s) 640
RsaI GTAC 7 cut(s) 67, 296, 331, 750, 851, 932, 1143
RsaNI GTAC 7 cut(s) 66, 295, 330, 749, 850, 931, 1142
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 9 cut(s) 60, 164, 308, 356, 365, 729, 830, 969, 1007
SatI GCNGC 1 cut(s) 710
Sau3AI GATC 4 cut(s) 640, 950, 1024, 1108
Sau96I GGNCC 2 cut(s) 473, 912
ScaI AGTACT 1 cut(s) 1143
ScrFI CCNGG 2 cut(s) 630, 794
SduI GDGCHC 1 cut(s) 942
SfcI CTRYAG 1 cut(s) 946
SinI GGWCC 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 4 cut(s) 149, 163, 380, 1246
SmoI CTYRAG 4 cut(s) 149, 163, 380, 1246
SpeI ACTAGT 1 cut(s) 1231
Sse9I AATT 9 cut(s) 40, 61, 268, 433, 900, 958, 1010, 1060, 1125
SsiI CCGC 1 cut(s) 183
SspMI CTAG 3 cut(s) 1170, 1232, 1312
StyD4I CCNGG 2 cut(s) 628, 792
TaqI TCGA 3 cut(s) 237, 739, 1057
TasI AATT 9 cut(s) 40, 61, 268, 433, 900, 958, 1010, 1060, 1125
TatI WGTACW 3 cut(s) 294, 930, 1141
TfiI GAWTC 6 cut(s) 122, 172, 595, 634, 1219, 1271
Tru1I TTAA 9 cut(s) 60, 164, 308, 356, 365, 729, 830, 969, 1007
Tru9I TTAA 9 cut(s) 60, 164, 308, 356, 365, 729, 830, 969, 1007
TscAI CASTG 1 cut(s) 431
TseFI GTSAC 3 cut(s) 32, 510, 1234
TseI GCWGC 1 cut(s) 709
Tsp45I GTSAC 3 cut(s) 32, 510, 1234
TspDTI ATGAA 3 cut(s) 1131, 1168, 1284
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 1 cut(s) 431
Van91I CCANNNNNTGG 1 cut(s) 921
Vha464I CTTAAG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 473
XapI RAATTY 5 cut(s) 40, 268, 433, 958, 1060
XbaI TCTAGA 1 cut(s) 1169
XmiI GTMKAC 1 cut(s) 1092
XspI CTAG 3 cut(s) 1170, 1232, 1312
ZrmI AGTACT 1 cut(s) 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.